EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
All TALEN records: 439 TALEN records match.
(Click on ID to see details)

Note: not including untested EENs targeting endogenous zebrafish genes constructed by an NIH-supported project, which are released in Addgene (list of these
TALENs or ZFNs).


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TNA001 tATCAAGATTCTCn(16)GAGAATCTTGATa
aTAGTTCTAAGAGn(16)CTCTTAGAACTAt
    12+[16]+12
(artificial) (std.) +235 WT (N/A) 20660643 R
TNA002 tCCCAGAAGTAAAn(16)TTTACTTCTGGGa
aGGGTCTTCATTTn(16)AAATGAAGACCCt
    12+[16]+12
(artificial) (std.) +235 WT (N/A) 20660643 R No
TNA003 tCCGGATGCTCCTn(18)AGGAGCATCCGGa
aGGCCTACGAGGAn(18)TCCTCGTAGGCCt
    12+[18]+12
(artificial) (std.) +235 WT (N/A) 20660643 R Low
TNA004 tAGACAAACCACAn(18)TGTGGTTTGTCTa
aTCTGTTTGGTGTn(18)ACACCAAACAGAt
    12+[18]+12
(artificial) (std.) +235 WT (N/A) 20660643 R No
TNA005 tACCCCTCTCCGCTn(18)AGCGGAGAGGGGTa
aTGGGGAGAGGCGAn(18)TCGCCTCTCCCCAt
    13+[18]+13
(artificial) (std.) +235 WT (N/A) 20660643 R
TNA006 (N/A)
    18+[15-30]+18
(artificial) +235 WT (N/A) 20660643 R
TNA007 (N/A)
    24+[13-30]+24
(artificial) +235 WT (N/A) 20660643 R
TNA008
(T-M)
tATAAACCCCCTCCAACCAGGTGCTAA
aTATTTGGGGGAGGTTGGTCCACGATT
    26
(artificial) (N*; NS-N; others) +297 WT (N/A) 20699274 R
TNA007B
(T-M)
tGCATCTCCCCCTACTGTACACCAC
aCGTAGAGGGGGATGACATGTGGTG
    24
(artificial) (NS-N; others) +297 WT (N/A) 20699274 R
TNA008B
(T-M-N)
tATAAACCCCCTCCAACCAGGTGCTAA
aTATTTGGGGGAGGTTGGTCCACGATT
    26
(artificial) (N*; NS-N; others) 4+288(N) WT (N/A) 20699274 R
TNA008C
(T-D-N)
TTAGCACCTGGTTGGAGGGGGTTTATa...tATAAACCCCCTCCAACCAGGTGCTAA
AATCGTGGACCAACCTCCCCCAAATAt...aTATTTGGGGGAGGTTGGTCCACGATT
    26+[x+2]+26
(artificial) (N*; NS-N; others) 4+288(N) WT (N/A) 20699274 R
TNA008D tATAAACCCCCTCCAACCAGGTGCTAAn(x)TTAGCACCTGGTTGGAGGGGGTTTATa
aTATTTGGGGGAGGTTGGTCCACGATTn(x)AATCGTGGACCAACCTCCCCCAAATAt
    26+[x]+26
(artificial) (N*; NS-N; others) +297 WT (N/A) 20699274 R
TNA008E/TNA007C tATAAACCCCCTCCAACCAGGTGCTAAn(x)GTGGTGTACAGTAGGGGGAGATGCa
aTATTTGGGGGAGGTTGGTCCACGATTn(x)CACCACATGTCATCCCCCTCTACGt
    26+[x]+24
(artificial) (N*; NS-N; others) +297, +297 WT (N/A) 20699274 R
TNA009 tATAAATACCTTCTn(2-24)AGAAGGTATTTATa
aTATTTATGGAAGAn(2-24)TCTTCCATAAATAt
    13+[2-24]+13
(artificial) (others) +28, +39, +50, +63, +79, +95 WT (N/A) 21179091 R
TN0001 cGGAGCCATCTGGCCGGGTtggctggttataACCGCGCAGATTCTGTTCa
gCCTCGGTAGACCGGCCCAaccgaccaatatTGGCGCGTCTAAGACAAGt
    18+[12]+18
human, NTF3, 5'-Up G C (NK) +63, +28 WT (N/A) NHEJ, NHEJ-ODC 21179091 R
TN0002 tCTTCATTACACCTg...cATACAGTCAGTatcaattctggaagaa
aGAAGTAATGTGGAc...gTATGTCAGTCAtagttaagaccttctt
    13+[16]+11
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0002B tctTCATTACACCTGCAGCTc...tACAGTCAGTATCAattctggaagaa
agaAGTAATGTGGACGTCGAg...aTGTCAGTCATAGTtaagaccttctt
    17+[12]+13
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0002C tcttcattACACCTGCAGCTCTc...cAGTATCAATTCTGGAAGaa
agaagtaaTGTGGACGTCGAGAg...gTCATAGTTAAGACCTTCtt
    14+[16]+17
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0002D tcttcattACACCTGCAGCTCTCATt...tATCAATTCTGGAAGAa
agaagtaaTGTGGACGTCGAGAGTAa...aTAGTTAAGACCTTCTt
    17+[16]+15
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0003 tGCTGGTCATCCTCATcctgataaactgcAAAAGGCTGAAGAGCatgactgaca
aCGACCAGTAGGAGTAggactatttgacgTTTTCCGACTTCTCGtactgactgt
    15+[13]+15
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TN0003B tgctGGTCATCCTCATCCTgataaactgcaaAAGGCTGAAGAGCatgactgaca
acgaCCAGTAGGAGTAGGActatttgacgttTTCCGACTTCTCGtactgactgt
    15+[12]+13
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TN0003C tgctggtCATCCTCATCCTGATaaactgcaaaAGGCTGAAGAGCATGactgaca
acgaccaGTAGGAGTAGGACTAtttgacgtttTCCGACTTCTCGTACtgactgt
    15+[10]+15
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TN0003D tgctggtcatCCTCATCCTGATAAACTgcaaaaggctgaAGAGCATGACTGACa
acgaccagtaGGAGTAGGACTATTTGAcgttttccgactTCTCGTACTGACTGt
    17+[12]+14
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TNA010 tCCCTTTATCTCTcgaatcccgggtacgaAGAGATAAAGGGa
aGGGAAATAGAGAgcttagggcccatgctTCTCTATTTCCCt
    12+[16]+12
(artificial) (NS-N) +286 WT (unnamed) 21262818 R
TNA008F tGCATCTCCCCCTACTGTACACCACc...cTTAGCACCTGGTTGGAGGGGGTTTATa
aCGTAGAGGGGGATGACATGTGGTGg...gAATCGTGGACCAACCTCCCCCAAATAt
    24+[19]+26
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA011 tATAAGGAACGTGCTGCTactcatcctagtcctgtTGCTGCCAAGCTATTTAATa
aTATTCCTTGCACGACGAtgagtaggatcaggacaACGACGGTTCGATAAATTAt
    17+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA012 tATTAAATAGCTTGGCAGCAa...tTGCTGCCAAGCTATTTAATa
aTAATTTATCGAACCGTCGTt...aACGACGGTTCGATAAATTAt
    19+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA013 tATGACACCCGGTGTGGGactcatcctagtcctgtTGCTGCCAAGCTATTTAATa
aTACTGTGGGCCACACCCtgagtaggatcaggacaACGACGGTTCGATAAATTAt
    17+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA014 tACTGTTGACCCAATGCGactcatcctagtcctgtTGCTGCCAAGCTATTTAATa
aTGACAACTGGGTTACGCtgagtaggatcaggacaACGACGGTTCGATAAATTAt
    17+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA015 tGCATATCCCCCAGCCAGACAAACa...tTGCTGCCAAGCTATTTAATa
aCGTATAGGGGGTCGGTCTGTTTGt...aACGACGGTTCGATAAATTAt
    23+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA016 tAATAACAATCAACCCACGTGGTTa...tTGCTGCCAAGCTATTTAATa
aTTATTGTTAGTTGGGTGCACCAAt...aACGACGGTTCGATAAATTAt
    23+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA017 tTCCAACCCAACCCTATCTTTCAa...tTGCTGCCAAGCTATTTAATa
aAGGTTGGGTTGGGATAGAAAGTt...aACGACGGTTCGATAAATTAt
    22+[17]+19
(artificial) (NS-N; others) +297 WT GG(Y) 21459844 R
TNA018 tGTCTACCAAGAACACCCTTAGGAa...tTGCTGCCAAGCTATTTAATa
aCAGATGGTTCTTGTGGGAATCCTt...aACGACGGTTCGATAAATTAt
    23+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA019 tTCCGCAAGCAAGCGTGGAATTAAAa...tTGCTGCCAAGCTATTTAATa
aAGGCGTTCGTTCGCACCTTAATTTt...aACGACGGTTCGATAAATTAt
    24+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA020 tACCTGGCAAGTGAGCAGCCCCACa...tTGCTGCCAAGCTATTTAATa
aTGGACCGTTCACTCGTCGGGGTGt...aACGACGGTTCGATAAATTAt
    23+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TN0004 tATAAGGAACGTGCTGCTactcatcctagtcctgtTGCTGCCAAGCTATTTAATa
aTATTCCTTGCACGACGAtgagtaggatcaggacaACGACGGTTCGATAAATTAt
    17+[17]+19
yeast, URA3 G (N*; others) +297 WT GG(Y) NHEJ, HR 21459844 R
TN0005 tATGACACCCGGTGTGGGtttagatgacaagggagaCGCATTGGGTCAACAGTa
aTACTGTGGGCCACACCCaaatctactgttccctctGCGTAACCCAGTTGTCAt
    17+[18]+17
yeast, URA3 G (others) +297 WT GG(Y) NHEJ, HR 21459844 R
TN0006 tGCATATCCCCCAGCCAGACAAACc...aAACCACGTGGGTTGATTGTTATTa
aCGTATAGGGGGTCGGTCTGTTTGg...tTTGGTGCACCCAACTAACAATAAt
    23+[20]+23
yeast, LYS2 G (N*; others) +297 WT GG(Y) NHEJ 21459844 R
TN0007 tTCCAACCCAACCCTATCTTTCACa...tTCCTAAGGGTGTTCTTGGTAGACa
aAGGTTGGGTTGGGATAGAAAGTGt...aAGGATTCCCACAAGAACCATCTGt
    22+[18]+23
yeast, LYS2 G (others) +297 WT GG(Y) NHEJ 21459844 R
TN0008 tCCGCAAGCAAGCGTGGAATTAAAa...gGTGGGGCTGCTCACTTGCCAGGTa
aGGCGTTCGTTCGCACCTTAATTTt...cCACCCCGACGAGTGAACGGTCCAt
    24+[19]+23
yeast, ADE2 G (others) +297 WT GG(Y) NHEJ 21459844 R
TN0009 tGCATACCTAATCATTATgctgaggatttggaaAGGGTGTTTATTCCTCATGGa
aCGTATGGATTAGTAATAcgactcctaaaccttTCCCACAAATAAGGAGTACCt
    17+[15]+20
human, HPRT1 G C (std.) +231 WT GG(V&B) 21493687 R
TN0010 tCCATTCCTATGACTGTAGATtttatcagactgaagAGCTATTGTGTGAGTATa
aGGTAAGGATACTGACATCTAaaatagtctgacttcTCGATAACACACTCATAt
    20+[15]+17
human, HPRT1 G C (std.) +231 WT GG(V&B) NHEJ, HR 21493687 R
TN0011 tGGATTATGCCTGGCACCATTAAAGAAAATa...tATAGATACAGAAGCGTCATCAAAGCATGCCa
aCCTAATACGGACCGTGGTAATTTCTTTTAt...aTATCTATGTCTTCGCAGTAGTTTCGTACGGt
    29+[28]+30
human, CFTR G C (others) +231 WT GG(V&B) 21493687 R
TN0012 tGGCACCATTAAAGAAAATATCATc...tAGATACAGAAGCGTCATCAAAGCATGCCa
aCCGTGGTAATTTCTTTTATAGTAg...aTCTATGTCTTCGCAGTAGTTTCGTACGGt
    23+[25]+28
human, CFTR G C (std.) +231 WT GG(V&B) (Weak) 21493687 R Low
TN0013 tATTTCGACGCTCATTCTctggccatggacttcttgAGCATCGGCTTCCGGGa
aTAAAGCTGCGAGTAAGAgaccggtacctgaagaacTCGTAGCCGAAGGCCCt
    17+[18]+16
zebrafish, hey2 G C (std.) +231 WT GG(V&B) 21493687 R
TN0014 tCCTGACCATTCATCAGCCGTCTt...cAAGATCCTTCTGATGGCCGAGGGCa
aGGACTGGTAAGTAGTCGGCAGAa...gTTCTAGGAAGACTACCGGCTCCCGt
    22+[24]+24
fruitfly, w G (others) +231 WT GG(V&B) 21493687 R
TN0015 tCCATGGCCAACACTTGTCACTACTTTc...aAGATACCCAGATCATATGAAGCGGCa
aGGTACCGGTTGTGAACAGTGATGAAAg...tTCTATGGGTCTAGTATACTTCGCCGt
    26+[25]+25
GFP G (std.) +231 WT GG(V&B) 21493687 R
TN0016 tACAACTACAACAGCCACAACGTCTa...aAGAACGGCATCAAGGTGAACTTCAAGa
aTGTTGATGTTGTCGGTGTTGCAGAt...tTCTTGCCGTAGTTCCACTTGAAGTTCt
    24+[21]+27
eGFP G (std.) +231 WT GG(V&B) 21493687 R
TN0017 tCGCAAAGAATTTCTTTAATt...cATCTTCGTTAAGTTTCCCGTGTa
aGCGTTTCTTAAAGAAATTAa...gTAGAAGCAATTCAAAGGGCACAt
    19+[15]+22
Plasmodium, PMV G (std.) +231 WT GG(V&B) 21493687 R
TN0018 tATTATTGGCCTTAACCATACTCTt...tATGTCAGAAAAATGTTTTACATGGa
aTAATAACCGGAATTGGTATGAGAa...aTACAGTCTTTTTACAAAATGTACCt
    23+[15]+24
Plasmodium, PMV G (std.) +231 WT GG(V&B) 21493687 R
TN0019 tCCATCTCCTCAAGGATGTTc...cAAGAACATTGTGAAGAGTCTAGa
aGGTAGAGGAGTTCCTACAAg...gTTCTTGTAACACTTCTCAGATCt
    19+[15]+22
thale cress, TT4 G (std.) +231 WT GG(V&B) 21493687 R
TN0020 tGTCGTCTTCTGCACTacctccggcgtcgacATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAtggaggccgcagctgTACGGACCACGACTGAt
    15+[15]+16
thale cress, TT4 G (std.) +231 WT GG(V&B) 21493687 R
TN0021 tCCGGATGCTCCTCTTgacaaggtctgtattgtcAGTTGTGGTTTGTCTa
aGGCCTACGAGGAGAActgttccagacataacagTCAACACCAAACAGAt
    15+[18]+15
thale cress, ADH1 G (std.) +231 WT GG(V&B) NHEJ, HR 21493687 R
TN0022 tACTGAATAATCAACACTtgggaatggtggttcAATGGGAGGATCGGTTCTATa
aTGACTTATTAGTTGTGAacccttaccaccaagTTACCCTCCTAGCCAAGATAt
    17+[15]+20
tobacco, SuRB G (std.) +231 WT GG(V&B) 21493687 R
TN0023 tACCTCATCAGGAACATGTTctacctatgattcccAGTGGCGGGGCTTTCAAAGa
aTGGAGTAGTCCTTGTACAAgatggatactaagggTCACCGCCCCGAAAGTTTCt
    19+[15]+19
tobacco, SuRB G (std.) +231 WT GG(V&B) 21493687 R
TNA002B tCCCAGAAGTAAAn(x)TTTACTTCTGGGa
aGGGTCTTCATTTn(x)AAATGAAGACCCt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA004B tAGACAAACCACAn(x)TGTGGTTTGTCTa
aTCTGTTTGGTGTn(x)ACACCAAACAGAt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA021 tGGCACTGCTAACCCTn(x)AGGGTTAGCAGTGCCa
aCCGTGACGATTGGGAn(x)TCCCAATCGTCACGGt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA022 tCGGTTTGCTATCGTTn(x)AACGATAGCAAACCGa
aGCCAAACGATAGCAAn(x)TTGCTATCGTTTGGCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA023 tCCCAGAAGTAAACATn(x)ATGTTTACTTCTGGGa
aGGGTCTTCATTTGTAn(x)TACAAATGAAGACCCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA003B tCCGGATGCTCCTn(x)AGGAGCATCCGGa
aGGCCTACGAGGAn(x)TCCTCGTAGGCCt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA024 tACCCCTCTCCn(x)GGAGAGGGGTa
aTGGGGAGAGGn(x)CCTCTCCCCAt
    10+[x]+10
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA025 tCTTGTCCGCATGATTn(x)AATCATGCGGACAAGa
aGAACAGGCGTACTAAn(x)TTAGTACGCCTGTTCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA026 tATTCCCCACGAGCTCTn(x)AGAGCTCGTGGGGAATa
aTAAGGGGTGCTCGAGAn(x)TCTCGAGCACCCCTTAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA027 tAGACAGGAAGTGGAGCn(x)GCTCCACTTCCTGTCTa
aTCTGTCCTTCACCTCGn(x)CGAGGTGAAGGACAGAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA001B tATCAAGATTCTCn(x)GAGAATCTTGATa
aTAGTTCTAAGAGn(x)CTCTTAGAACTAt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA028 tAGACAAACCACAACTn(x)AGTTGTGGTTTGTCTa
aTCTGTTTGGTGTTGAn(x)TCAACACCAAACAGAt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA005B tACCCCTCTCCGCTn(x)AGCGGAGAGGGGTa
aTGGGGAGAGGCGAn(x)TCGCCTCTCCCCAt
    13+[x]+13
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA029 tCCGGATGCTCCTCTTn(x)AAGAGGAGCATCCGGa
aGGCCTACGAGGAGAAn(x)TTCTCCTCGTAGGCCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA030 tACCCCTCTCCGCTTCTn(x)AGAAGCGGAGAGGGGTa
aTGGGGAGAGGCGAAGAn(x)TCTTCGCCTCTCCCCAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TN0024 tgggatCCAGCCTGATGGAACt...gATTTGCAGTTGGAAAGAatcaa
accctaGGTCGGACTACCTTGa...cTAAACGTCAACCTTTCTtagtt
    15+[17]+17
worm, ben-1 G (std.) +63 WT (N/A) NHEJ 21700836 R
TN0024B tgggatCCAGCCTGATGGAACt...tGCAGTTGGAAAGAATCAa
accctaGGTCGGACTACCTTGa...aCGTCAACCTTTCTTAGTt
    15+[21]+17
worm, ben-1 G (std.) +63 WT (N/A) NHEJ 21700836 R No
TN0024C tGGGATCCAGCCTGATg...gATTTGCAGTTGGAAAGAatcaa
aCCCTAGGTCGGACTAc...cTAAACGTCAACCTTTCTtagtt
    15+[22]+17
worm, ben-1 G (std.) +63 WT (N/A) NHEJ 21700836 R No
TN0024D tgggatCCAGCCTGATGGAACTTa...tGCAGTTGGAAAGAATCAa
accctaGGTCGGACTACCTTGAAt...aCGTCAACCTTTCTTAGTt
    17+[19]+17
worm, ben-1 G (std.) +63 WT (N/A) (Inactive) 21700836 R No
TN0025 tCCCCTCCACCCCACAGTggggccactagggacAGGATTGGTGACAGAAAa
aGGGGAGGTGGGGTGTCAccccggtgatccctgTCCTAACCACTGTCTTTt
    17+[15]+17
human, PPP1R12C, intron G C (std.) +63 WT; ELD+KKR (N/A) NHEJ, HR 21738127 R
TN0025-T002 tTCCACCCAGCCCTCAGTgctctgctgaggcAGGATGGGTGGCTGGAAa
aAGGTGGGTCGGGAGTCAcgagacgactccgTCCTACCCACCGACCTTt
    17+[13]+17
human, [intergenic] C +63 ELD+KKR (N/A) NHEJ 21738127 R
TN0025-T003 cCCCCTCCACCCCAAAAAt...aAATATGAGTTATAGAAAa
gGGGGAGGTGGGGTTTTTa...tTTATACTCAATATCTTTt
    17+[24]+17
human, [intergenic] C +63 ELD+KKR (N/A) NHEJ 21738127 R
TN0026 tCAGACGCTGGCACTccgcacccccagctcccagagcACGGCTGCAAGGGCCa
aGTCTGCGACCGTGAggcgtgggggtcgagggtctcgTGCCGACGTTCCCGGt
    14+[22]+15
human, PITX3 G C (std.) +63 WT (N/A) NHEJ, HR 21738127 R
TN0027 tACGCCGTGGAAAGGCCcgtatgagcggccccgcccGTAGATCATCCCCGa
aTGCGGCACCTTTCCGGgcatactcgccggggcgggCATCTAGTAGGGGCt
    16+[19]+14
human, PITX3, 3'-UTR G C (std.) +63 WT (N/A) NHEJ, HR 21738127 R
TN0028 tCACCTGCAGCTGCCCAGacctggcacccaggagAGGAGCAGGCAGGGTCa
aGTGGACGTCGACGGGTCtggaccgtgggtcctcTCCTCGTCCGTCCCAGt
    17+[16]+16
human, POU5F1, intron G C (std.) +63 WT (N/A) NHEJ, HR 21738127 R
TN0029 tCTGGGCTCTCCCATgcattcaaactgaggtgcctgcCCTTCTAGGAATGGGGGa
aGACCCGAGAGGGTAcgtaagtttgactccacggacgGGAAGATCCTTACCCCCt
    14+[22]+17
human, POU5F1, 3'-UTR G C (std.) +63 WT (N/A) NHEJ, HR 21738127 R
TNA031 tATAATTAATAATCCACTTn(x)AAGTGGATTATTAATTATa
aTATTAATTATTAGGTGAAn(x)TTCACCTAATAATTAATAt
    18+[x]+18
(artificial) (NS-N) AvrBs4-BB, AvrBs4-NH, AvrBs4-EH, AvrBs4-EC, AvrBs4-NC KV+EA (N/A) 21813459 R
TNA031B tATAATTAATAATCCACTTaggctcgagttacAGAGGGTTAGGTTTATAGa
aTATTAATTATTAGGTGAAtccgagctcaatgTCTCCCAATCCAAATATCt
    18+[13]+18
(artificial) (NS-N) +17 KV+EA (N/A) 21813459 R
TN0030 tACACGTTTCGTGTTCGGAgccgctttaacccacTCTGTGGAAGTGCTCAGCa
aTGTGCAAAGCACAAGCCTcggcgaaattgggtgAGACACCTTCACGAGTCGt
    18+[15]+18
human, IL2RG G C (std.) +47, +17 KV+EA (N/A) NHEJ 21813459 R
TN0031 tGTGGGCAACATGCTGGTCatcctcatcctgataAACTGCAAAAGGCTGAAGa
aCACCCGTTGTACGACCAGtaggagtaggactatTTGACGTTTTCCGACTTCt
    18+[15]+18
human, CCR5 G C (std.) +47, +17 KV+EA (N/A) NHEJ 21813459 R
TN0031-T002 tGTGGGCAACATGCTGGTCgtcctcatcttaataAACTGCAAAAAGCTGAAGt
aCACCCGTTGTACGACCAGcaggagtagaattatTTGACGTTTTTCGACTTCa
    18+[15]+18
human, CCR2 G C +47, +17 KV+EA (N/A) NHEJ 21813459 R Low
TN0032 tTCCTGCCCAGCTCCATttccttctcctggaactACCAGAACAACACTGAa
aAGGACGGGTCGAGGTAaaggaagaggaccttgaTGGTCTTGTTGTGACTt
    16+[17]+16
rat, IGG2B G (std.) +63 ELD+KKR (N/A) NHEJ 21822240 R
TN0032-T002 tTCCTGACCAGCTCCAAgcataaaagcatAACAGTAGAAAATTGAt
aAGGACTGGTCGAGGTTcgtattttcgtaTTGTCATCTTTTAACTa
    16+[12]+16
rat, [intergenic] +63 ELD+KKR (N/A) NHEJ 21822240 R
TN0033 tCTTCCGTTTCCACATCCaccacatcccaacagagcAGCGGGAGCAGCAGTAAa
aGAAGGCAAAGGTGTAGGtggtgtagggttgtctcgTCGCCCTCGTCGTCATTt
    17+[18]+17
zebrafish, hey2 G C (others) +63 WT REAL NHEJ 21822241 R
TN0034 tCGTCCAATAGCTTCTcagtcacgcacgcctgtGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAgtcagtgcgtgcggacaCTCAAAGACGAGAAAt
    15+[17]+15
zebrafish, gria3a G C (std.) +63 WT REAL NHEJ 21822241 R
TN0034B tCGTCCAATAGCTTCTcagtcacgcacgcctgTGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAgtcagtgcgtgcggacACTCAAAGACGAGAAAt
    15+[16]+16
zebrafish, gria3a G C (std.) +63 WT REAL NHEJ 21822241 R
TN0034C tCGTCCAATAGCTTCTCagtcacgcacgcctgtGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAGtcagtgcgtgcggacaCTCAAAGACGAGAAAt
    16+[16]+15
zebrafish, gria3a G C (std.) +63 WT REAL NHEJ 21822241 R
TN0035 tGTTATTTTCTCCCCTaaggatcctgcgggcATTTTTGAGCTGGTGGa
aCAATAAAAGAGGGGAttcctaggacgcccgTAAAAACTCGACCACCt
    15+[15]+16
zebrafish, tnikb G C (std.) +63 Sharkey-AS+R UA NHEJ 21822242 R
TN0035B tGTTATTTTCTCCCCTaaggatcctgcgggcATTTTTGAGCTGGTGGa
aCAATAAAAGAGGGGAttcctaggacgcccgTAAAAACTCGACCACCt
    15+[15]+16
zebrafish, tnikb G C (NK) +63 Sharkey-AS+R UA NHEJ 21822242 R
TN0036 tACACACACTCCAGTCTgatggagacagctgATGGTGAGTCACACAAACa
aTGTGTGTGAGGTCAGActacctctgtcgacTACCACTCAGTGTGTTTGt
    16+[14]+18
zebrafish, dip2a G C (std.) +63 Sharkey-AS+R UA NHEJ 21822242 R
TN0037 tGCATCAACCCCATCATCtatgcctttgtcGGGGAGAAGTTCAGAAACTa
aCGTAGTTGGGGTAGTAGatacggaaacagCCCCTCTTCAAGTCTTTGAt
    17+[12]+19
human, CCR5 G C (N/A) (N/A) (N/A) NHEJ 21983922 R
TN0038 tCCCACTTCTCAAgcgcttaagacaagaacaAGGAGGAGGAGTa
aGGGTGAAGAGTTcgcgaattctgttcttgtTCCTCCTCCTCAt
    12+[18]+12
thale cress, PDS3 G (others) Hax3 (N/A) idTALE 22271303 R
TN0039 tCCTTTTACCAGAt...aTAAATTAGTTGGa
aGGAAAATGGTCTa...tATTTAATCAACCt
    12+[36]+12
thale cress, PDS3 G (others) Hax3 (N/A) idTALE 22271303 R
TNA032 tCCCACTTCTCAAn(x)TTGAGAAGTGGGa
aGGGTGAAGAGTTn(x)AACTCTTCACCCt
    12+[x]+12
(artificial) (NS-N; others) Hax3 (N/A) idTALE 22271303 R
TNA033 tATATAAGCACGTATCTn(x)AGATACGTGCTTATATa
aTATATTCGTGCATAGAn(x)TCTATGCACGAATATAt
    16+[x]+16
(artificial) (N*; NS-N; others) N0/1/2/3/4-C0, N3-C1/2/3/4/5 WT (N/A) 22301904 R
TNA033B nATATAAGCACGTATCTn(16)AGATACGTGCTTATATn
nTATATTCGTGCATAGAn(16)TCTATGCACGAATATAn
    16+[16]+16
(artificial) (N*; NS-N; others) N2/3/4-C0, N3-C1/2 WT (N/A) 22301904 R
TN0040 tAGCAACCTCAAACAGACACCATg...cTGTGGAGAAGTCTGCCGTTa
aTCGTTGGAGTTTGTCTGTGGTAc...gACACCTCTTCAGACGGCAAt
    22+[15]+19
human, HBB G C (N*; others) +31, +63, +31, +31 WT (N/A) NHEJ 22301904 R
TN0041A tCGCCACCATggtgagcaagggcgagGAGCTGTTCa
aGCGGTGGTAccactcgttcccgctcCTCGACAAGt
    9+[16]+9
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041B tGGTGCCCATcctggtcgagctggacGGCGACGTAa
aCCACGGGTAggaccagctcgacctgCCGCTGCATt
    9+[16]+9
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041C tCTGCACCACcggcaagctgcccgtgCCCTGGCCCa
aGACGTGGTGgccgttcgacgggcacGGGACCGGGt
    9+[16]+9
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041D tGGAGTACAActacaacagccacaacGTCTATATCa
aCCTCATGTTgatgttgtcggtgttgCAGATATAGt
    9+[16]+9
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041E tTCAGCGTGTCcggcgagggcgagggcGATGCCACCTa
aAGTCGCACAGgccgctcccgctcccgCTACGGTGGAt
    10+[16]+10
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041F tGCCACCTACGgcaagctgaccctgaaGTTCATCTGCa
aCGGTGGATGCcgttcgactgggacttCAAGTAGACGt
    10+[16]+10
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041G tGGCCCACCCTcgtgaccaccctgaccTACGGCGTGCa
aCCGGGTGGGAgcactggtgggactggATGCCGCACGt
    10+[16]+10
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041H tTCAAGATCCGccacaacatcgaggacGGCAGCGTGCa
aAGTTCTAGGCggtgttgtagctcctgCCGTCGCACGt
    10+[16]+10
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041I tAGAGGATCCACcggtcgccaccatggtGAGCAAGGGCGa
aTCTCCTAGGTGgccagcggtggtaccaCTCGTTCCCGCt
    11+[16]+11
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041J tCCGGCGAGGGCgagggcgatgccacctACGGCAAGCTGa
aGGCCGCTCCCGctcccgctacggtggaTGCCGTTCGACt
    11+[16]+11
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041K tGACCTACGGCGtgcagtgcttcagccgCTACCCCGACCa
aCTGGATGCCGCacgtcacgaagtcggcGATGGGGCTGGt
    11+[16]+11
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041L tCCGCCACAACAtcgaggacggcagcgtGCAGCTCGCCGa
aGGCGGTGTTGTagctcctgccgtcgcaCGTCGAGCGGCt
    11+[16]+11
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041M tCCTGGTCGAGCTggacggcgacgtaaacGGCCACAAGTTCa
aGGACCAGCTCGAcctgccgctgcatttgCCGGTGTTCAAGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041N tCAGCCGCTACCCcgaccacatgaagcagCACGACTTCTTCa
aGTCGGCGATGGGgctggtgtacttcgtcGTGCTGAAGAAGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041O tCTTCAAGTCCGCcatgcccgaaggctacGTCCAGGAGCGCa
aGAAGTTCAGGCGgtacgggcttccgatgCAGGTCCTCGCGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041P tCAAGGAGGACGGcaacatcctggggcacAAGCTGGAGTACa
aGTTCCTCCTGCCgttgtaggaccccgtgTTCGACCTCATGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041Q tCAAGGTGAACTTcaagatccgccacaacATCGAGGACGGCa
aGTTCCACTTGAAgttctaggcggtgttgTAGCTCCTGCCGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041R tCCACCGGTCGCCAccatggtgagcaagggCGAGGAGCTGTTCa
aGGTGGCCAGCGGTggtaccactcgttcccGCTCCTCGACAAGt
    13+[16]+13
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041S tTCAGCGTGTCCGGcgagggcgagggcgatGCCACCTACGGCAa
aAGTCGCACAGGCCgctcccgctcccgctaCGGTGGATGCCGTt
    13+[16]+13
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041T tTCAGCCGCTACCCcgaccacatgaagcagCACGACTTCTTCAa
aAGTCGGCGATGGGgctggtgtacttcgtcGTGCTGAAGAAGTt
    13+[16]+13
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041U tACAACAGCCACAAcgtctatatcatggccGACAAGCAGAAGAa
aTGTTGTCGGTGTTgcagatatagtaccggCTGTTCGTCTTCTt
    13+[16]+13
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041V tGGTGCCCATCCTGGtcgagctggacggcgaCGTAAACGGCCACAa
aCCACGGGTAGGACCagctcgacctgccgctGCATTTGCCGGTGTt
    14+[16]+14
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041W tGCCCGAAGGCTACGtccaggagcgcaccatCTTCTTCAAGGACGa
aCGGGCTTCCGATGCaggtcctcgcgtggtaGAAGAAGTTCCTGCt
    14+[16]+14
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041X tGAACCGCATCGAGCtgaagggcatcgacttCAAGGAGGACGGCAa
aCTTGGCGTAGCTCGacttcccgtagctgaaGTTCCTCCTGCCGTt
    14+[16]+14
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041Y tCAAGATCCGCCACAacatcgaggacggcagCGTGCAGCTCGCCGa
aGTTCTAGGCGGTGTtgtagctcctgccgtcGCACGTCGAGCGGCt
    14+[16]+14
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041Z tGTTCACCGGGGTGGTgcccatcctggtcgagCTGGACGGCGACGTAa
aCAAGTGGCCCCACCAcgggtaggaccagctcGACCTGCCGCTGCATt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AA tGCCCATCCTGGTCGAgctggacggcgacgtaAACGGCCACAAGTTCa
aCGGGTAGGACCAGCTcgacctgccgctgcatTTGCCGGTGTTCAAGt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AB tGTCCGGCGAGGGCGAgggcgatgccacctacGGCAAGCTGACCCTGa
aCAGGCCGCTCCCGCTcccgctacggtggatgCCGTTCGACTGGGACt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AC tCATCTGCACCACCGGcaagctgcccgtgcccTGGCCCACCCTCGTGa
aGTAGACGTGGTGGCCgttcgacgggcacgggACCGGGTGGGAGCACt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AD tCTATATCATGGCCGAcaagcagaagaacggcATCAAGGTGAACTTCa
aGATATAGTACCGGCTgttcgtcttcttgccgTAGTTCCACTTGAAGt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AE tACGGCGTGCAGTGCTTcagccgctaccccgacCACATGAAGCAGCACGa
aTGCCGCACGTCACGAAgtcggcgatggggctgGTGTACTTCGTCGTGCt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AF tACCCCGACCACATGAAgcagcacgacttcttcAAGTCCGCCATGCCCGa
aTGGGGCTGGTGTACTTcgtcgtgctgaagaagTTCAGGCGGTACGGGCt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AG tCCGCCATGCCCGAAGGctacgtccaggagcgcACCATCTTCTTCAAGGa
aGGCGGTACGGGCTTCCgatgcaggtcctcgcgTGGTAGAAGAAGTTCCt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AH tTCAAGGAGGACGGCAAcatcctggggcacaagCTGGAGTACAACTACAa
aAGTTCCTCCTGCCGTTgtaggaccccgtgttcGACCTCATGTTGATGTt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AI tACAACTACAACAGCCAcaacgtctatatcatgGCCGACAAGCAGAAGAa
aTGTTGATGTTGTCGGTgttgcagatatagtacCGGCTGTTCGTCTTCTt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AJ tCACCGGGGTGGTGCCCAtcctggtcgagctggaCGGCGACGTAAACGGCCa
aGTGGCCCCACCACGGGTaggaccagctcgacctGCCGCTGCATTTGCCGGt
    17+[16]+17
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AK tAAACGGCCACAAGTTCAgcgtgtccggcgagggCGAGGGCGATGCCACCTa
aTTTGCCGGTGTTCAAGTcgcacaggccgctcccGCTCCCGCTACGGTGGAt
    17+[16]+17
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AL tGCAGTGCTTCAGCCGCTaccccgaccacatgaaGCAGCACGACTTCTTCAa
aCGTCACGAAGTCGGCGAtggggctggtgtacttCGTCGTGCTGAAGAAGTt
    17+[16]+17
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AM tCAGCGTGTCCGGCGAGGGcgagggcgatgccaccTACGGCAAGCTGACCCTGa
aGTCGCACAGGCCGCTCCCgctcccgctacggtggATGCCGTTCGACTGGGACt
    18+[16]+18
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AN tGAAGTTCATCTGCACCACcggcaagctgcccgtgCCCTGGCCCACCCTCGTGa
aCTTCAAGTAGACGTGGTGgccgttcgacgggcacGGGACCGGGTGGGAGCACt
    18+[16]+18
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AO tCGTGACCACCCTGACCTAcggcgtgcagtgcttcAGCCGCTACCCCGACCACa
aGCACTGGTGGGACTGGATgccgcacgtcacgaagTCGGCGATGGGGCTGGTGt
    18+[16]+18
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AP tCATGGCCGACAAGCAGAAgaacggcatcaaggtgAACTTCAAGATCCGCCACa
aGTACCGGCTGTTCGTCTTcttgccgtagttccacTTGAAGTTCTAGGCGGTGt
    18+[16]+18
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AQ tTCACCGGGGTGGTGCCCATc...cGGCGACGTAAACGGCCACAa
aAGTGGCCCCACCACGGGTAg...gCCGCTGCATTTGCCGGTGTt
    19+[16]+19
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AR tGCACCACCGGCAAGCTGCCc...cCTCGTGACCACCCTGACCTa
aCGTGGTGGCCGTTCGACGGg...gGAGCACTGGTGGGACTGGAt
    19+[16]+19
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AS tTCAAGTCCGCCATGCCCGAa...gCGCACCATCTTCTTCAAGGa
aAGTTCAGGCGGTACGGGCTt...cGCGTGGTAGAAGAAGTTCCt
    19+[16]+19
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AT tATATCATGGCCGACAAGCAg...gGTGAACTTCAAGATCCGCCa
aTATAGTACCGGCTGTTCGTc...cCACTTGAAGTTCTAGGCGGt
    19+[16]+19
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AU tTCATCTGCACCACCGGCAAGc...cCCACCCTCGTGACCACCCTGa
aAGTAGACGTGGTGGCCGTTCg...gGGTGGGAGCACTGGTGGGACt
    20+[16]+20
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AV tGAAGTTCGAGGGCGACACCCt...aGCTGAAGGGCATCGACTTCAa
aCTTCAAGCTCCCGCTGTGGGa...tCGACTTCCCGTAGCTGAAGTt
    20+[16]+20
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0042 tACCTATTATTACTTTATggggcagcagcctggaaAAGTACTTGGGGACCAa
aTGGATAATAATGAAATAccccgtcgtcggaccttTTCATGAACCCCTGGTt
    17+[17]+16
human, ABL1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0043 tGTGTCTTGGGATGAGTGggtcagtgttctggtgCTCACAGGATGGCTGGCa
aCACAGAACCCTACTCACccagtcacaagaccacGAGTGTCCTACCGACCGt
    17+[16]+17
human, AKT2 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0044 tCCTGTGGCTCCTGCCGCtgctgctttccacggcAGCTGTGGGCTCCGGGa
aGGACACCGAGGACGGCGacgacgaaaggtgccgTCGACACCCGAGGCCCt
    17+[16]+16
human, ALK G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0045 tATGTACGCCTCCCTGGGctcgggtccggtcgccCCTTTGCCCGCTTCTGTa
aTACATGCGGAGGGACCCgagcccaggccagcggGGAAACGGGCGAAGACAt
    17+[16]+17
human, APC, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0046 tGAATTGGGATGCTGTTTttaggtattctattcaaaTTTATTTTACTGTCTTTa
aCTTAACCCTACGACAAAaatccataagataagtttAAATAAAATGACAGAAAt
    17+[18]+17
human, ATM G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0047 tCCCTCACCATGAGTAGCgctatgttggtgacttGCCTCCCGGACCCCAGCa
aGGGAGTGGTACTCATCGcgatacaaccactgaaCGGAGGGCCTGGGGTCGt
    17+[16]+17
human, AXIN2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0048 tGTGCGATCTCCAAGCACtgaggggcagaaactcCCGGATCGGGCGCTGCCa
aCACGCTAGAGGTTCGTGactccccgtctttgagGGCCTAGCCCGCGACGGt
    17+[16]+17
human, BAX G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0049 tTTTCAAGTGAAGACAAAatggcctcgccggctgACAGCTGTATCCAGTTCa
aAAAGTTCACTTCTGTTTtaccggagcggccgacTGTCGACATAGGTCAAGt
    17+[16]+17
human, BCL6, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0050 tACAATTGAACAATGCCTcagctatacatttacatCAGATTATTGGGAGCCTa
aTGTTAACTTGTTACGGAgtcgatatgtaaatgtaGTCTAATAACCCTCGGAt
    17+[17]+17
human, BMPR1A, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0051 tCCGAAGCTGACAGATGGgtattctttgacggggGGTAGGGGCGGAACCTGa
aGGCTTCGACTGTCTACCcataagaaactgccccCCATCCCCGCCTTGGACt
    17+[16]+17
human, BRCA1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0052 tTAGACTTAGGTAAGTAAtgcaatatggtagactGGGGAGAACTACAAACTa
aATCTGAATCCATTCATTacgttataccatctgaCCCCTCTTGATGTTTGAt
    17+[16]+17
human, BRCA2 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0053 tCTGCAATAAAAAATGGCctccaacaaaactacaTTGGTAAGTTAATGAAAa
aGACGTTATTTTTTACCGgaggttgttttgatgtAACCATTCAATTACTTTt
    17+[16]+17
human, CBX3, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0054 tGGAGCTTTCAGCGGTGGgggagcgggtgttcgcgGCCGAAGCCCTCCTGAa
aCCTCGAAAGTCGCCACCccctcgcccacaagcgcCGGCTTCGGGAGGACTt
    17+[17]+16
human, CBX8, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0055 tGGAACACCAGCTCCTGTgctgcgaagtggaaaccatCCGCCGCGCGTACCCCGa
aCCTTGTGGTCGAGGACAcgacgcttcacctttggtaGGCGGCGCGCATGGGGCt
    17+[19]+17
human, CCND1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0056 tGCTTAGCGTCCTGCGACagtacaacatccagaaGAAGGAGATTGTGGTGAa
aCGAATCGCAGGACGCTGtcatgttgtaggtcttCTTCCTCTAACACCACTt
    17+[16]+17
human, CDC73 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0057 tGCTGCAGGTACCCCGGAtcccctgacttgcgagGGACGCATTCGGGCCGCa
aCGACGTCCATGGGGCCTaggggactgaacgctcCCTGCGTAAGCCCGGCGt
    17+[16]+17
human, CDH1 G C (std.) +63 WT FLASH 22484455 R No
TN0058 tCCCTTGATCTGAGAAtggctacctctcgataTGAGCCAGTGGCTGAAa
aGGGAACTAGACTCTTaccgatggagagctatACTCGGTCACCGACTTt
    15+[16]+16
human, CDK4, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0059 tGGCGTCGGGCCTGGGCtccccgtccccctgctcGGCGGGCAGTGAGGAGGa
aCCGCAGCCCGGACCCGaggggcagggggacgagCCGCCCGTCACTCCTCCt
    16+[17]+17
human, CHD4, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0060 tGTGTTGGAAGAAGATGGcagatccaggaatgatGAGTCTTTTTGGCGAGGa
aCACAACCTTCTTCTACCgtctaggtccttactaCTCAGAAAAACCGCTCCt
    17+[16]+17
human, CHD7, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0061 tCCAGCGTGGACAATGGctactcaaggtttgtgTCATTAAATCTTTAGTTa
aGGTCGCACCTGTTACCgatgagttccaaacacAGTAATTTAGAAATCAAt
    16+[16]+17
human, CTNNB1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0062 tAATATCACAATGAGTTCaggcttatggagccaagaAAAAGTCACTTCACCCTa
aTTATAGTGTTACTCAAGtccgaatacctcggttctTTTTCAGTGAAGTGGGAt
    17+[18]+17
human, CYLD, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0063 tCACACGGAGGACGCGatggctcccaagaaacGCCCAGAAACCCAGAAGa
aGTGTGCCTCCTGCGCtaccgagggttctttgCGGGTCTTTGGGTCTTCt
    15+[16]+17
human, DDB2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0064 tCCGGCCGGCGCCATGAagtgagaagggggctgGGGGTCGCGCTCGCTa
aGGCCGGCCGCGGTACTtcactcttcccccgacCCCCAGCGCGAGCGAt
    16+[16]+15
human, ERCC2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0065 tCCGGGATCGCCATGGGAactcaatagaaaatcctcaTCTTCTCACTTTGTTTCa
aGGCCCTAGCGGTACCCTtgagttatcttttaggagtAGAAGAGTGAAACAAAGt
    17+[19]+17
human, ERCC5 G C (std.) +63 WT FLASH 22484455 R No
TN0066 tGGCGTCCACGGGTGAGTatggtggaactgcggtcGCGCCGGCGGTAGCCGGa
aCCGCAGGTGCCCACTCAtaccaccttgacgccagCGCGGCCGCCATCGGCCt
    17+[17]+17
human, EWSR1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0067 tGACCCAGGCAGGACACAtgcaggccaaaaaacgcTATTTCATCCTGCTCTCa
aCTGGGTCCGTCCTGTGTacgtccggttttttgcgATAAAGTAGGACGAGAGt
    17+[17]+17
human, EXT1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0068 tTCCTCCCAGGGGGATGTcctgcgcctcagggtcCGGTGGTGGCCTGCGGCa
aAGGAGGGTCCCCCTACAggacgcggagtcccagGCCACCACCGGACGCCGt
    17+[16]+17
human, EXT2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0069 tGCTTTTAGAATAATCATgggccagactgggaagAAATCTGAGAAGGGACCa
aCGAAAATCTTATTAGTAcccggtctgacccttcTTTAGACTCTTCCCTGGt
    17+[16]+17
human, EZH2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0070 tAGGCGCCAAGGCCATGTccgactcgtgggtcccGAACTCCGCCTCGGGCCa
aTCCGCGGTTCCGGTACAggctgagcacccagggCTTGAGGCGGAGCCCGGt
    17+[16]+17
human, FANCA, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0071 tGAAGGGACATCACCTTTtcgctttttccaagatgGCTCAAGATTCAGTAGa
aCTTCCCTGTAGTGGAAAagcgaaaaaggttctacCGAGTTCTAAGTCATCt
    17+[17]+16
human, FANCC, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0072 tGCCCCGGCATGGCGACAccggacgcggggctcccTGGGGCTGAGGGCGTGGa
aCGGGGCCGTACCGCTGTggcctgcgccccgagggACCCCGACTCCCGCACCt
    17+[17]+17
human, FANCE, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0073 tTCGCGCACCTCATGGaatcccttctgcagcaCCTGGATCGCTTTTCCGa
aAGCGCGTGGAGTACCttagggaagacgtcgtGGACCTAGCGAAAAGGCt
    15+[16]+17
human, FANCF, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0074 tCGGCCACCATGTCCCgccagaccacctctgtGGGCTCCAGCTGCCTGGa
aGCCGGTGGTACAGGGcggtctggtggagacaCCCGAGGTCGACGGACCt
    15+[16]+17
human, FANCG, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0075 tCCCCAGAACAGCACTATgggcttctcttccgagctGTGCAGCCCCCAGGGCCa
aGGGGTCTTGTCGTGATAcccgaagagaaggctcgaCACGTCGGGGGTCCCGGt
    17+[18]+17
human, FES, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0076 tCTGCTCCCCACCGAGGAcctctgcatgcaggcaTGAATCCCAGGAGCCTa
aGACGAGGGGTGGCTCCTggagacgtacgtccgtACTTAGGGTCCTCGGAt
    17+[16]+16
human, FGFR1 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0077 tGTACCGAGCACTTCGGCtcctcgcgcgctcgcgtCCCCTCGTGCGGGCTCCa
aCATGGCTCGTGAAGCCGaggagcgcgcgagcgcaGGGGAGCACGCCCGAGGt
    17+[17]+17
human, FH, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0078 tCTCCAAGGCACCATGAAtgccatcgtggctctctgCCACTTCTGCGAGCTCCa
aGAGGTTCCGTGGTACTTacggtagcaccgagagacGGTGAAGACGCTCGAGGt
    17+[18]+17
human, FLCN, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0079 tCCGGAGGCCATGCCGGCg...aGCTGCCGCTGCTCGGTa
aGGCCTCCGGTACGGCCGc...tCGACGGCGACGAGCCAt
    17+[21]+16
human, FLT3, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0080 tGCAGCGGGGCGCCGCGCtgtgcctgcgactgtggctCTGCCTGGGACTCCTGGa
aCGTCGCCCCGCGGCGCGacacggacgctgacaccgaGACGGACCCTGAGGACCt
    17+[19]+17
human, FLT4, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0081 tCACCATGGCCGAGGCGcctcaggtggtggagaTCGACCCGGACTTCGa
aGTGGTACCGGCTCCGCggagtccaccacctctAGCTGGGCCTGAAGCt
    16+[16]+15
human, FOXO1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0082 tCTCCGCTCGAAGTGGAGctggacccggagttcgagCCCCAGAGCCGTCCGCGa
aGAGGCGAGCTTCACCTCgacctgggcctcaagctcGGGGTCTCGGCAGGCGCt
    17+[18]+17
human, FOXO3 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0083 tCCTCTGAGACGCCATGTtcaactcgatgaccccACCACCAATCAGTAGCTa
aGGAGACTCTGCGGTACAagttgagctactggggTGGTGGTTAGTCATCGAt
    17+[16]+17
human, GLI1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0084 tGGCGCAGACGCAGGGCacccggaggaaagtctgTTACTACTACGACGGTGa
aCCGCGTCTGCGTCCCGtgggcctcctttcagacAATGATGATGCTGCCACt
    16+[17]+17
human, HDAC1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0085 tGCGCTCACCTCCCTGCGgcctcctgaggtggtttgGTGGCCCCCTCCTCGCGa
aCGCGAGTGGAGGGACGCcggaggactccaccaaacCACCGGGGGAGGAGCGCt
    17+[18]+17
human, HDAC2 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0086 tCCTCAACTATGACCTCAaccggccaggattccaCCACAACCAGGCAGCGAa
aGGAGTTGATACTGGAGTtggccggtcctaaggtGGTGTTGGTCCGTCGCTt
    17+[16]+17
human, HDAC6, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0087 tGAGCGCACGCGGTGAGGgcgcggggcagccgtcCACTTCAGCCCAGGGACa
aCTCGCGTGCGCCACTCCcgcgccccgtcggcagGTGAAGTCGGGTCCCTGt
    17+[16]+17
human, HMGA2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0088 tCCGTGCTCCTCCACCCCcgctggatcgagcccacCGTCATGTTTCTCTACGa
aGGCACGAGGAGGTGGGGgcgacctagctcgggtgGCAGTACAAAGAGATGCt
    17+[17]+17
human, HOXA13 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0089 tGGGCACGGTGATGGCcaccactggggccctgGGCAACTACTACGTGGa
aCCCGTGCCACTACCGgtggtgaccccgggacCCGTTGATGATGCACCt
    15+[16]+16
human, HOXA9, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0090 tCCAGCAGATCATGTCATgacgacttcgctgctcctGCATCCACGCTGGCCGGa
aGGTCGTCTAGTACAGTActgctgaagcgacgaggaCGTAGGTGCGACCGGCCt
    17+[18]+17
human, HOXC13, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0091 tTGACGAGTGCGGCCAGagcgcagccagcatgtaCCTGCCGGGCTGCGCCTa
aACTGCTCACGCCGGTCtcgcgtcggtcgtacatGGACGGCCCGACGCGGAt
    16+[17]+17
human, HOXD11 G C (std.) +63 WT FLASH 22484455 R No
TN0092 tGCGGGCAGACGGCGGGGgcgccggtggcgccccgGCCTCTTCCTCCTCCTCa
aCGCCCGTCTGCCGCCCCcgcggccaccgcggggcCGGAGAAGGAGGAGGAGt
    17+[17]+17
human, HOXD13 G C (std.) +63 WT FLASH 22484455 R No
TN0093 tCTGAAAAAGACTCTGCAtgggaatggcctgcctTACGATGACAGAAATGGa
aGACTTTTTCTGAGACGTacccttaccggacggaATGCTACTGTCTTTACCt
    17+[16]+17
human, JAK2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0094 tACCGCGATGAGAGGCGCtcgcggcgcctgggattttCTCTGCGTTCTGCTCCTa
aTGGCGCTACTCTCCGCGagcgccgcggaccctaaaaGAGACGCAAGACGAGGAt
    17+[19]+17
human, KIT, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0095 tGAAAATGACTGAATATAaacttgtggtagttggaGCTGGTGGCGTAGGCAa
aCTTTTACTGACTTATATttgaacaccatcaacctCGACCACCGCATCCGTt
    17+[17]+16
human, KRAS, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0096 tAGGGTCCCCGGCGCCAGgccacccggccgtcagCAGCATGCAGGGTAAGGa
aTCCCAGGGGCCGCGGTCcggtgggccggcagtcGTCGTACGTCCCATTCCt
    17+[16]+17
human, MAP2K4 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0097 tCCAAGCGCGAAAACCCCggatggtgaggagcaggTACTGGCCCGGCAGCGa
aGGTTCGCGCTTTTGGGGcctaccactcctcgtccATGACCGGGCCGTCGCt
    17+[17]+16
human, MDM2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0098 tTATTATTACATGGCTTTgccttactgaggcttcATCTTGTCCTCTGGTCCa
aATAATAATGTACCGAAAcggaatgactccgaagTAGAACAGGAGACCAGGt
    17+[16]+17
human, MET G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0099 tCTGGCGCCAAAATGTCGttcgtggcaggggttaTTCGGCGGCTGGACGAGa
aGACCGCGGTTTTACAGCaagcaccgtccccaatAAGCCGCCGACCTGCTCt
    17+[16]+17
human, MLH1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0100 tGAGGAGGTTTCGACATGgcggtgcagccgaaggAGACGCTGCAGTTGGAGa
aCTCCTCCAAAGCTGTACcgccacgtcggcttccTCTGCGACGTCAACCTCt
    17+[16]+17
human, MSH2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0101 tCACTGTCGGCGGCCATGacaccgctcgtctcccgcCTGAGTCGTCTGTGGGTa
aGTGACAGCCGCCGGTACtgtggcgagcagagggcgGACTCAGCAGACACCCAt
    17+[18]+17
human, MUTYH, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0102 tGCTTAGACGCTGGATTTttttcgggtagtggaaAACCAGGTAAGCACCGAa
aCGAATCTGCGACCTAAAaaaagcccatcaccttTTGGTCCATTCGTGGCTt
    17+[16]+17
human, MYC G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0103 tCCCGCAGGGAGCGGACAtggactacgactcgtaCCAGCACTATTTCTACGa
aGGGCGTCCCTCGCCTGTacctgatgctgagcatGGTCGTGATAAAGATGCt
    17+[16]+17
human, MYCL1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0104 tGCCGAGCTGCTCCACgtccaccatgccgggcATGATCTGCAAGAACCCa
aCGGCTCGACGAGGTGcaggtggtacggcccgTACTAGACGTTCTTGGGt
    15+[16]+17
human, MYCN, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0105 tGAGGAGCCGGACCGAtgtggaaactgctgccCGCCGCGGGCCCGGCa
aCTCCTCGGCCTGGCTacacctttgacgacggGCGGCGCCCGGGCCGt
    15+[16]+15
human, NBN, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0106 tCTTTACTGATAATGTCAagttcaggttatcctcCCAACCAAGGAGCATTCa
aGAAATGACTATTACAGTtcaagtccaataggagGGTTGGTTCCTCGTAAGt
    17+[16]+17
human, NCOR1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0107 tGGAGGGCCACTGAGCcccgctacccgccccaCAGCCTTTCCTACCCa
aCCTCCCGGTGACTCGgggcgatgggcggggtGTCGGAAAGGATGGGt
    15+[16]+15
human, NCOR2 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0108 tCGGCGCATGAAGGAGGTactcctcattttcgttCTCTCTCTCTGTGCCCCa
aGCCGCGTACTTCCTCCAtgaggagtaaaagcaaGAGAGAGAGACACGGGGt
    17+[16]+17
human, NTRK1, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0109 tTGCGCTCGGGGCGGCCAtgtcggccggcgaggtCGAGCGCCTAGTGTCGGa
aACGCGAGCCCCGCCGGTacagccggccgctccaGCTCGCGGATCACAGCCt
    17+[16]+17
human, PDGFRA, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0110 tCTGCAGGACACCATGCGgcttccgggtgcgatgCCAGCTCTGGCCCTCAAa
aGACGTCCTGTGGTACGCcgaaggcccacgctacGGTCGAGACCGGGAGTTt
    17+[16]+17
human, PDGFRB, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0111 tGAGTACTCCGCCTCTACcccggctgaagcccgcCCCCGCCGCCACCTATTa
aCTCATGAGGCGGAGATGgggccgacttcgggcgGGGGCGGCGGTGGATAAt
    17+[16]+17
human, PHF8 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0112 tCGGGTGTTGCATCCATGgagcgagctgagagctcgAGGTGAGCGGGGCTCGCa
aGCCCACAACGTAGGTACctcgctcgactctcgagcTCCACTCGCCCCGAGCGt
    17+[18]+17
human, PMS2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0113 tGGAACTGCTTAATAGaaacaggcttgtaattGTGAGTCCGCGCTGCa
aCCTTGACGAATTATCtttgtccgaacattaaCACTCAGGCGCGACGt
    15+[16]+15
human, PTCH1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0114 tCCCAGACATGACAGCCatcatcaaagagatcgTTAGCAGAAACAAAAGGa
aGGGTCTGTACTGTCGGtagtagtttctctagcAATCGTCTTTGTTTTCCt
    16+[16]+17
human, PTEN, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0115 tGGCATGGCCAGCAACAGcagctcctgcccgacacCTGGGGGCGGGCACCTCa
aCCGTACCGGTCGTTGTCgtcgaggacgggctgtgGACCCCCGCCCGTGGAGt
    17+[17]+17
human, RARA, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0116 tGCTGGGTGAGAAGGGCtgtggctgcgttttagaGAAGCGTTGGGTACTGGa
aCGACCCACTCTTCCCGacaccgacgcaaaatctCTTCGCAACCCATGACCt
    16+[17]+17
human, RBBP5 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0117 tGCGGGACGTGCGGGAGCggctgcaggcgtgggaGCGCGCGTTCCGACGGCa
aCGCCCTGCACGCCCTCGccgacgtccgcaccctCGCGCGCAAGGCTGCCGt
    17+[16]+17
human, RECQL4 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0118 tCAGAATACAGTTATGGCcacccaggtaatggggCAGTCTTCTGGAGGAGGa
aGTCTTATGTCAATACCGgtgggtccattaccccGTCAGAAGACCTCCTCCt
    17+[16]+17
human, REST, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0119 tGAGTTCTGCCGGCCGCCggctcccgcaggggccaGGGCGAAGTTGGCGCCGa
aCTCAAGACGGCCGGCGGccgagggcgtccccggtCCCGCTTCAACCGCGGCt
    17+[17]+17
human, RET G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0120 tTCTTTATTTCCAGCAATgtctcaggctgtgcagACAAACGGAACTCAACCa
aAGAAATAAAGGTCGTTAcagagtccgacacgtcTGTTTGCCTTGAGTTGGt
    17+[16]+17
human, RNF2, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0121 tTCAGGAGGAAGCGATGGcttcagacagcatattTGAGTCATTTCCTTCGTa
aAGTCCTCCTTCGCTACCgaagtctgtcgtataaACTCAGTAAAGGAAGCAt
    17+[16]+17
human, RUNX1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0122 tCTCCTTGAGGCGCCGGTtgccggccacaaccctTGGCGGAGCCTGCCTGCa
aGAGGAACTCCGCGGCCAacggccggtgttgggaACCGCCTCGGACGGACGt
    17+[16]+17
human, SDHB G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0123 tGTTGCTGAGGTGACTTCagtgggactgggagttggtGCCTGCGGCCCTCCGGa
aCAACGACTCCACTGAAGtcaccctgaccctcaaccaCGGACGCCGGGAGGCCt
    17+[19]+16
human, SDHC G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0124 tCAGGAACGAGATGGCGGttctctggaggctgagtGCCGTTTGCGGTGCCCTa
aGTCCTTGCTCTACCGCCaagagacctccgactcaCGGCAAACGCCACGGGAt
    17+[17]+17
human, SDHD, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0125 tGCAGAGGACAAAAGCATgtcttcccttcctgggTGCATTGGTTTGGATGCa
aCGTCTCCTGTTTTCGTAcagaagggaaggacccACGTAACCAAACCTACGt
    17+[16]+17
human, SETDB1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0126 tTACGCGGCGGGGCTGTCgccgtacgcggacaagggCAAGTGCGGCCTCCCGGa
aATGCGCCGCCCCGACAGcggcatgcgcctgttcccGTTCACGCCGGAGGGCCt
    17+[18]+17
human, SIRT6 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0127 tTTGGTAAGAACATGTCGtccatcttgccattcacGCCGCCAGTTGTGAAGa
aAACCATTCTTGTACAGCaggtagaacggtaagtgCGGCGGTCAACACTTCt
    17+[17]+16
human, SMAD2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0128 tGGTGACGGCGGCAACATgtctgtggctttcgcggCCCCGAGGCAGCGAGGCa
aCCACTGCCGCCGTTGTAcagacaccgaaagcgccGGGGCTCCGTCGCTCCGt
    17+[17]+17
human, SS18, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0129 tGGCGCCTCAGAAGCAcggcggtgggggagggGGCGGCTCGGGGCCCa
aCCGCGGAGTCTTCGTgccgccaccccctcccCCGCCGAGCCCCGGGt
    15+[16]+15
human, SUZ12, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0130 tCATGTCTCATGCGGCCGaaccagctcgggatggCGTAGAGGCCAGCGCGGa
aGTACAGAGTACGCCGGCttggtcgagccctaccGCATCTCCGGTCGCGCCt
    17+[16]+17
human, TFE3, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0131 tCGGGGGCTGCTCAGGGGcctgtggccgctgcacaTCGTCCTGTGGACGCGTa
aGCCCCCGACGAGTCCCCggacaccggcgacgtgtAGCAGGACACCTGCGCAt
    17+[17]+17
human, TGFBR2 G C (std.) +63 WT FLASH 22484455 R No
TN0132 tTCCGCCCGCCCAGGATGgaggcgcccgccagcgcGCAGACCCCGCACCCGCa
aAGGCGGGCGGGTCCTACctccgcgggcggtcgcgCGTCTGGGGCGTGGGCGt
    17+[17]+17
human, TLX3, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0133 tTGCCGTCCCAAGCAATGgatgatttgatgctgtcCCCGGACGATATTGAACa
aACGGCAGGGTTCGTTACctactaaactacgacagGGGCCTGCTATAACTTGt
    17+[17]+17
human, TP53 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0134 tCCTGGTCCACCATGGCcaaaccaacaagcaaagATTCAGGCTTGAAGGAGa
aGGACCAGGTGGTACCGgtttggttgttcgtttcTAAGTCCGAACTTCCTCt
    16+[17]+17
human, TSC2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0135 tCTGGATCGCGGAGGGAAtgccccggagggcggaGAACTGGGACGAGGCCGa
aGACCTAGCGCCTCCCTTacggggcctcccgcctCTTGACCCTGCTCCGGCt
    17+[16]+17
human, VHL, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0136 tGGGCCAGAGATGGCGGCggccgacggggctttgCCGGAGGCGGCGGCTTTa
aCCCGGTCTCTACCGCCGccggctgccccgaaacGGCCTCCGCCGCCGAAAt
    17+[16]+17
human, XPA, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0137 tGCCCAGACAAGCAACATggctcggaaacgcgcggccGGCGGGGAGCCGCGGGGa
aCGGGTCTGTTCGTTGTAccgagcctttgcgcgccggCCGCCCCTCGGCGCCCCt
    17+[19]+17
human, XPC, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0138 tCTTCCTTCCTAGCACTATATAAAc...cTAAGCTCATCAAGCCTTCAAGCa
aGAAGGAAGGATCGTGATATATTTg...gATTCGAGTAGTTCGGAAGTTCGt
    23+[18]+22
rice, Os11N3, promoter G (std.) +297 WT GG-PCR NHEJ 22565958 R
TN0139 tATAAACCCCCTCCAACCAGGTGCTAAg...cAAAGCAAACTCAAGTAGTAGCTGa
aTATTTGGGGGAGGTTGGTCCACGATTc...gTTTCGTTTGAGTTCATCATCGACt
    26+[19]+23
rice, Os11N3, promoter G (N*; NS-N; others) +297 WT GG-PCR NHEJ 22565958 R
TN0140 tACCACCACTAATCCGTgcccctatgcggtaaATGTCTTTGACTTGACCa
aTGGTGGTGATTAGGCAcggggatacgccattTACAGAAACTGAACTGGt
    16+[15]+17
fruitfly, y G (std.) +63 Sharkey-AS+R UA NHEJ 22624882 R
TN0141 (N/A)
    
fruitfly, CG9797 G +63 Sharkey-AS+R UA NHEJ 22624882 R
TN0146 tGCTCAAACCCGACGAGCatcaccacatcgaggacgAGCACGTGAGGGCGCCCa
aCGAGTTTGGGCTGCTCGtagtggtgtagctcctgcTCGTGCACTCCCGCGGGt
    17+[18]+17
zebrafish, myod1 G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0147 tATTTCTGCGCAGAAGTGgccccaccaagcctgctGGAGGACATACAGAATTa
aTAAAGACGCGTCTTCACcggggtggttcggacgaCCTCCTGTATGTCTTAAt
    17+[17]+17
zebrafish, jak3 G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0145 tCAGCCTTGGTCACATCTcattcagacagtgaaaACATCGGAGGCTTTTCTa
aGTCGGAACCAGTGTAGAgtaagtctgtcactttTGTAGCCTCCGAAAAGAt
    17+[16]+17
zebrafish, phf6 G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0144 tGGAGTGTATGAAAACTGgtcatgtcaggacagaGAAAAGGAGCTTCAGCa
aCCTCACATACTTTTGACcagtacagtcctgtctCTTTTCCTCGAAGTCGt
    17+[16]+16
zebrafish, phf6, ATG G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0142 tTCTTTTCTATCCCATGAcgatgcatcgcacaacAAGGATCAAGATTACAGa
aAGAAAAGATAGGGTACTgctacgtagcgtgttgTTCCTAGTTCTAATGTCt
    17+[16]+17
zebrafish, bmi1a, ATG G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0143 tACCTGCACAGGATGCAgactgaggaggcacagGAAATGTCCCAGATAACa
aTGGACGTGTCCTACGTctgactcctccgtgtcCTTTACAGGGTCTATTGt
    16+[16]+17
zebrafish, ikzf1, ATG G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0150 tACAATACTCCCACTGAAatgacagatgcagacagACTCATGGACAGTTGGTa
aTGTTATGAGGGTGACTTtactgtctacgtctgtcTGAGTACCTGTCAACCAt
    17+[17]+17
zebrafish, epas1b G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0149 tCAGAGCGGCGTAAGGAGaaatctcgtgatgccgCGCGCTGCAGACGGAGTa
aGTCTCGCCGCATTCCTCtttagagcactacggcGCGCGACGTCTGCCTCAt
    17+[16]+17
zebrafish, epas1b G C (std.) +63 WT; ELD+KKR FLASH (Inactive) 22684503 R No
TN0148 tCCGGCAGACATCGTGAAggtggccatcgagtggCCTGGGGCCTTCCCCAAa
aGGCCGTCTGTAGCACTTccaccggtagctcaccGGACCCCGGAAGGGGTTt
    17+[16]+17
zebrafish, elmo1 G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0150-T002 tACGCTACTCCCACTGAAatgacagatgcagacagACTCATGGACAGTTGGTa
aTGCGATGAGGGTGACTTtactgtctacgtctgtcTGAGTACCTGTCAACCAt
    17+[17]+17
zebrafish, epas1b [mutated] G C +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0151 tTTGATGGATCTCTGAGCggcccgcagatctgacAAACTCGCGCTGAAGCGa
aAACTACCTAGAGACTCGccgggcgtctagactgTTTGAGCGCGACTTCGCt
    17+[16]+17
zebrafish, fh G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0152 tACTGGAGTTGTCACTGAaaagaaaaggtagggaTTCCCGTTTTTGCTTGGa
aTGACCTCAACAGTGACTtttcttttccatccctAAGGGCAAAAACGAACCt
    17+[16]+17
zebrafish, hif1ab, exon-intron G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0153 tCGGAGCGCAGGAAGGAGaagtccagggatgcagCGCGATCTCGCAGGGGAa
aGCCTCGCGTCCTTCCTCttcaggtccctacgtcGCGCTAGAGCGTCCCCTt
    17+[16]+17
zebrafish, hif1ab G C (std.) +63 WT; ELD+KKR FLASH (Inactive) 22684503 R No
TN0154 TTCGTTTATACGAGGAACcatgtcgagtgttttAGCGAGAATTTTATTCTA
AAGCAAATATGCTCCTTGgtacagctcacaaaaTCGCTCTTAAAATAAGAT
    18+[15]+18
zebrafish, ptpmt1 G C (std.) +63 WT FLASH (Inactive) 22684503 R No
TN0155 tGGTCCAAAATGAAAAAGtaagaggagtaattacCATGAATGAAGAATATGa
aCCAGGTTTTACTTTTTCattctcctcattaatgGTACTTACTTCTTATACt
    17+[16]+17
zebrafish, ptpmt1 G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0156 TTGAGCAGATCAGATTGGacactgtggacctgacAGGTGTTCCCAGTCTTGA
AACTCGTCTAGTCTAACCtgtgacacctggactgTCCACAAGGGTCAGAACT
    18+[16]+18
zebrafish, ptpmt1 G C (std.) +63 WT FLASH NHEJ 22684503 R
TN0157 tAGAAGAGCGGCATCCCGgcgatcaccatgaagaAGAGATACGGCACCAGGa
aTCTTCTCGCCGTAGGGCcgctagtggtacttctTCTCTATGCCGTGGTCCt
    17+[16]+17
zebrafish, slc6a3 G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0158A ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    19+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158B ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATcgatggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAgctacccctag
    19+[17]+11
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158C ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCgatggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGctacccctag
    19+[17]+12
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158D ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGatggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCtacccctag
    19+[17]+13
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158E ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGAtggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTacccctag
    19+[17]+14
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158F ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTAcccctag
    19+[17]+15
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158G ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCcctag
    19+[17]+17
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158H ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGgatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCctag
    19+[17]+18
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158I ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGAtc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCTag
    19+[17]+20
eGFP [mutated] G (std.) WT ICA HR 22740649 R
TN0158J ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGATc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCTAg
    19+[17]+21
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158K ntccagtagcAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtcatcgTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    11+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158L ntccagtagCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtcatcGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    12+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158M ntccagtaGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtcatCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    13+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158N ntccagtAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtcaTCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    14+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158O ntccaGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    16+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158P ntccAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    17+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158Q ntcCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
nagGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    18+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158R nTCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
nAGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    20+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0159 tACCAGTTCCAGCTTGCTTTa...gAAGTGACTGGACAACa
aTGGTCAAGGTCGAACGAAAt...cTTCACTGACCTGTTGt
    19+[24]+15
rat, Bmpr2 G (std.) +231 (N/A) GG(V&B) NHEJ 22749015 R
TN0160 tGTCGGACTGGATGCACg...cCGGCCGCCTCGCCGTCa
aCAGCCTGACCTACGTGc...gGCCGGCGGAGCGGCAGt
    16+[23]+16
cricket, Gb'lac2 G (std.) (N/A) (N/A) (N/A) NHEJ 22910363 R
TN0161 tACATGCTGCTGTCCGTctccatcgtgtgtgATGAATATTTTCTGCCa
aTGTACGACGACAGGCAgaggtagcacacacTACTTATAAAAGACGGt
    16+[14]+16
zebrafish, slc24a5 G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0162 tGGACAATTCATCCGGCATccaagcagaggtcagAGGGTGAGAAGGTGCGCa
aCCTGTTAAGTAGGCCGTAggttcgtctccagtcTCCCACTCTTCCACGCGt
    18+[15]+17
zebrafish, ryr3 G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0163 tATTTCAGACGGATGTTtcccagctgcaaagtaACAGTGACAGGACTAAATCCa
aTAAAGTCTGCCTACAAagggtcgacgtttcatTGTCACTGTCCTGATTTAGGt
    16+[16]+20
zebrafish, tbx6l G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0164 tGGACCATTCACCCTGCATccaagcagaggtcagAGGGTGAGAAGGTCAGa
aCCTGGTAAGTGGGACGTAggttcgtctccagtcTCCCACTCTTCCAGTCt
    18+[15]+16
zebrafish, ryr1a G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0165 tGTGAGCACCCAGCGGACCTCCTCTg...cATCTCTGACTGTTGTTCTGATa
aCACTCGTGGGTCGCCTGGAGGAGAc...gTAGAGACTGACAACAAGACTAt
    24+[18]+21
zebrafish, ponzr1 G C (std.) +63-Goldy, +231 WT GG(V&B) NHEJ, HR 23000899 R
TN0166 tTGCAACACTGAGCTCTGTAAACCTg...aACCTCAGGGTCCAGTCGGCAGCAGa
aACGTTGTGACTCGAGACATTTGGAc...tTGGAGTCCCAGGTCAGCCGTCGTCt
    24+[15]+24
zebrafish, crhr1, upstream G C (std.) +63-Goldy, +231 WT GG(V&B) NHEJ 23000899 R
TN0167 tACCCAGAAGACGTTTctgaggaactgattcAGGAGGCCACTCAAAGa
aTGGGTCTTCTGCAAAgactccttgactaagTCCTCCGGTGAGTTTCt
    15+[15]+16
zebrafish, msna G C (std.) +63-Goldy WT GG(V&B) NHEJ 23000899 R
TN0168 tCCACCAGAGAGTAACTacctatttctgggagACTATGTTGACAGAGGCa
aGGTGGTCTCTCATTGAtggataaagaccctcTGATACAACTGTCTCCGt
    16+[15]+17
zebrafish, ppp1cab G C (std.) +63-Goldy WT GG(V&B) NHEJ 23000899 R
TN0169 tCTCCTCAACATACATACTggagagttagttgacaAAGATGAATCATTTGTa
aGAGGAGTTGTATGTATGAcctctcaatcaactgtTTCTACTTAGTAAACAt
    18+[16]+16
zebrafish, cdh5 G C (std.) +63-Goldy WT GG(V&B) NHEJ 23000899 R
TN0170 tGTCAAATCTGCAGCTCCACGCTTc...cCAGAGTCAGAGGCAGAGGa
aCAGTTTAGACGTCGAGGTGCGAAg...gGTCTCAGTCTCCGTCTCCt
    23+[15]+18
zebrafish, crhr2, intron G C (std.) +63-Goldy WT GG(V&B) HR 23000899 R
TN0171 tATACATTTTACACACATATatatgaaactgacagtATTAATGGCCTGAACCTa
aTATGTAAAATGTGTGTATAtatactttgactgtcaTAATTACCGGACTTGGAt
    19+[16]+17
pig, SRY, 3'-downstream 1.4kb G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0172 tATATGAAACTGACAGTATtaatggcctgaacctAGCCAGAACTCAGATTGGGa
aTATACTTTGACTGTCATAattaccggacttggaTCGGTCTTGAGTCTAACCCt
    18+[15]+19
pig, SRY, 3'-downstream 1.5kb G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0173 tGCCCCCCCACACAGCTgagcccatgctgatggAGTACCCTGAGGCTATa
aCGGGGGGGTGTGTCGActcgggtacgactaccTCATGGGACTCCGATAt
    16+[16]+16
pig, RELA G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0174 tCCCACTCCCCTGGGGGCCTCTg...cCTCTCGGGGGACGAAGa
aGGGTGAGGGGACCCCCGGAGAc...gGAGAGCCCCCTGCTTCt
    21+[18]+16
pig, RELA G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R Low
TN0175 tCACCAACGGTCTCCTCTCGGGggacgaagacttctccTCCATTGCGGACATGGa
aGTGGTTGCCAGAGGAGAGCCCcctgcttctgaagaggAGGTAACGCCTGTACCt
    21+[16]+16
pig, RELA G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R Low
TN0176 tGGAACATGCATTCAACATcgccaagtatcagttAGGCATAGAGAAACTACTGGa
aCCTTGTACGTAAGTTGTAgcggttcatagtcaaTCCGTATCTCTTTGATGACCt
    18+[15]+20
pig, DMD G (std.) +63-Goldy, +231 WT GG(V&B) NHEJ, NHEJ-DisDel, NHEJ-Inv 23027955 R
TN0177 tCTATACCTAGGTCAAAAATg...cATGGCTGGATTGCAACAAACTa
aGATATGGATCCAGTTTTTAc...gTACCGACCTAACGTTGTTTGAt
    19+[15]+21
pig, DMD, intron-exon G (std.) +63-Goldy WT GG(V&B) NHEJ, NHEJ-DisDel, NHEJ-Inv 23027955 R
TN0178 tCGCCAAGTATCAGTTAGGCATa...aAGGTTAGTGCATTTTGGGa
aGCGGTTCATAGTCAATCCGTAt...tTCCAATCACGTAAAACCCt
    21+[21]+18
pig, DMD, exon-intron G (std.) +63-Goldy, +231 WT GG(V&B) 23027955 R No
TN0179 tCCTTCCAAGGCTTCTACATc...cACTTTTGATAAGACATTCTTGGTa
aGGAAGGTTCCGAAGATGTAg...gTGAAAACTATTCTGTAAGAACCAt
    19+[16]+23
pig, AMELY, Intron G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0180 tCTACATCAATCACTTGGCTttcacttttgataagACATTCTTGGTATGGATa
aGATGTAGTTAGTGAACCGAaagtgaaaactattcTGTAAGAACCATACCTAt
    19+[15]+17
pig, AMELY, Intron G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R No
TN0181 tCTGCGCTCCTTCAAAGTgtttaagatcaagcctgAGAAGAGGTGGCAGGACa
aGACGCGAGGAAGTTTCAcaaattctagttcggacTCTTCTCCACCGTCCTGt
    17+[17]+17
cow, GGTA1 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0182 tCTGCAAATACATACTTCATg...cATCTTTTACATCATGGTGGa
aGACGTTTATGTATGAAGTAc...gTAGAAAATGTAGTACCACCt
    19+[16]+19
pig, GGTA1 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0183 tGCGTTCCTTTAAAGTGTttgagatcaagtccgAGAAGAGGTGGCAAGACa
aCGCAAGGAAATTTCACAaactctagttcaggcTCTTCTCCACCGTTCTGt
    17+[15]+17
pig, GGTA1 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0184 tCCTTTCCTCCAGGGATCCCTc...cACTGGAGAAGCAACAGAGGAGCa
aGGAAAGGAGGTCCCTAGGGAg...gTGACCTCTTCGTTGTCTCCTCGt
    20+[15]+22
cow, ACAN G (std.) +63-Goldy, +231 WT GG(V&B) NHEJ 23027955 R
TN0185 tGCAATCCCAGGCTTCACCGTTg...gACGGAATGGGAACTTGCCTACa
aCGTTAGGGTCCGAAGTGGCAAc...cTGCCTTACCCTTGAACGGATGt
    21+[15]+21
cow, ACAN, intron-exon G (std.) +63-Goldy, +231 WT GG(V&B) 23027955 R Low
TN0186 tCCAGTGCAACAGCTCCACCTg...gGGCCTGTGATGGTGATCCTGa
aGGTCACGTTGTCGAGGTGGAc...cCCGGACACTACCACTAGGACt
    20+[16]+20
pig, LDLR G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0187 tCTCCTACAAGTGGATTTgtgatgggaacaccgAGTGCAAGGACGGGTCCGa
aGAGGATGTTCACCTAAAcactacccttgtggcTCACGTTCCTGCCCAGGCt
    17+[15]+18
pig, LDLR G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0188 tCCACTCCAGCTGGCGCTgcgacggagacactgACTGCAAGGACAAGTCTGACGa
aGGTGAGGTCGACCGCGAcgctgcctctgtgacTGACGTTCCTGTTCAGACTGCt
    17+[15]+21
pig, LDLR G (std.) +63-Goldy, +231 WT GG(V&B) 23027955 R No
TN0189 tGCAACAGCTCCACCTGCATCCCTg...gATGGTGATCCTGACTGCGAGGa
aCGTTGTCGAGGTGGACGTAGGGAc...cTACCACTAGGACTGACGCTCCt
    23+[16]+21
pig, LDLR G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0190 tACCTTCCTCCTCTCCGCTacccagccacttgcacATTCAAAAGCAGCTTAGa
aTGGAAGGAGGAGAGGCGAtgggtcggtgaacgtgTAAGTTTTCGTCGAATCt
    18+[16]+17
pig, RAG2 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0191 tGGTAATAACATGGCCTTAATt...cATTGATGAATTTTGATGGGCa
aCCATTATTGTACCGGAATTAa...gTAACTACTTAAAACTACCCGt
    20+[15]+20
pig, RAG2 G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0192 tCCCAAAGGTTCAGTGTTTtgtgttcaatgttgAGTACATGAATTGCACTTGGa
aGGGTTTCCAAGTCACAAAacacaagttacaacTCATGTACTTAACGTGAACCt
    18+[14]+20
pig, IL2RG G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0193 tCTACCAAACATTTGTTGTccagctccaggacccACGGGAACCCAGGAGGCAGGa
aGATGGTTTGTAAACAACAggtcgaggtcctgggTGCCCTTGGGTCCTCCGTCCt
    18+[15]+20
pig, IL2RG G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R No
TN0194 tCCCCTGCCCGGCTTTCTTCTctcacttcagctctgAGCCAGGTGAGGGGGa
aGGGGACGGGCCGAAAGAAGAgagtgaagtcgagacTCGGTCCACTCCCCCt
    20+[15]+15
pig, GHRHR, intron-exon G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0195 tCCCATCTCGCCGTCCAGGCTg...gATGGGCTGTTGTGCTGGCCGa
aGGGTAGAGCGGCAGGTCCGAc...cTACCCGACAACACGACCGGCt
    20+[15]+20
pig, GHRHR, exon-intron G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0196 tCCCTTTTTAGAAGTCAAGGTa...aAAGATCCAGGAGAGATTTTGGa
aGGGAAAAATCTTCAGTTCCAt...tTTCTAGGTCCTCTCTAAAACCt
    20+[15]+21
pig, MSTN G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0197 tGGATTATTGCACCCAAAAGATa...gCTCTGGAGAGTGTGAATTTGTa
aCCTAATAACGTGGGTTTTCTAt...cGAGACCTCTCACACTTAAACAt
    21+[16]+21
pig, MSTN G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0198 tCTAACTGTGGATTTTGAAGCTtttggatgggactggATTATTGCACCCAAAAGa
aGATTGACACCTAAAACTTCGAaaacctaccctgaccTAATAACGTGGGTTTTCt
    21+[15]+17
pig, MSTN G (std.) +63-Goldy, +231 WT GG(V&B) NHEJ 23027955 R Low
TN0199A tGTGATGAACACTCCACAGAATCTc...cCTCTAACTGTGGATTTTGAa
aCACTACTTGTGAGGTGTCTTAGAg...gGAGATTGACACCTAAAACTt
    23+[16]+19
cow, MSTN G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0199B tGTGATGAACACTCCACAGAATCTc...aACTGTGGATTTTGAAGCTTTTGGa
aCACTACTTGTGAGGTGTCTTAGAg...tTGACACCTAAAACTTCGAAAACCt
    23+[21]+23
cow, MSTN G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0200 tGCAAGAAGCGACCAAAACCTggaggaggatggaacACTGGGGGGAGCCGATa
aCGTTCTTCGCTGGTTTTGGAcctcctcctaccttgTGACCCCCCTCGGCTAt
    20+[15]+16
cow, PRNP G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R Low
TN0201 tCAATGGAACAAACCCAGTAAg...gAAGCATGTGGCAGGAGCTGCTGCa
aGTTACCTTGTTTGGGTCATTc...cTTCGTACACCGTCCTCGACGACGt
    20+[16]+23
cow, PRNP G (std.) +63-Goldy, +231 WT GG(V&B) 23027955 R No
TN0202 tGTTGAGTGACAATTTCTCCCTCCTGCAGGTt...gATGAGGGTTCTGTGGGTTGCTTTGGTGGTa
aCAACTCACTGTTAAAGAGGGAGGACGTCCAa...cTACTCCCAAGACACCCAACGAAACCACCAt
    30+[21]+29
pig, APOE, intron-exon G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0203 tGTTCCCCAGGCCTGGTTCATACTCTt...tACCAGGAGGTGGGAGCCTTGGGTACCa
aCAAGGGGTCCGGACCAAGTATGAGAa...aTGGTCCTCCACCCTCGGAACCCATGGt
    25+[18]+26
pig, HR G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0204 tGGATGAAACCGAAATTagaagtttctttgctagATATGGTTCAGTAAAAGa
aCCTACTTTGGCTTTAAtcttcaaagaaacgatcTATACCAAGTCATTTTCt
    16+[17]+17
pig, DAZL G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0205 tCCAAGCACTGTTTAAGTctggatgttggtcaaaACCCAATTTGATGATGTa
aGGTTCGTGACAAATTCAgacctacaaccagtttTGGGTTAAACTACTACAt
    17+[16]+17
cow, OFD1Y G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0206 tGGATCCACATGATCCTGTcatcagtcggttagccACACAGCTCTTTAAAAAAa
aCCTAGGTGTACTAGGACAgtagtcagccaatcggTGTGTCGAGAAATTTTTTt
    18+[16]+18
silk worm, BmBLOS2 G (std.) +63 EL+KK GG-PCR NHEJ, NHEJ-DisDel 23028749 R
TN0207 tCACCAAGTACGCAGATCTaaggagcctagccgcgAACCTGAACAAGACCCTTa
aGTGGTTCATGCGTCTAGAttcctcggatcggcgcTTGGACTTGTTCTGGGAAt
    18+[16]+18
silk worm, BmBLOS2 G (std.) +63 EL+KK GG-PCR NHEJ, NHEJ-DisDel 23028749 R
TNA034 tGTCGTCTTCTGCACTn(x)AGTGCAGAAGACGACa
aCAGCAGAAGACGTGAn(x)TCACGTCTTCTGCTGt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA034B tGTCGTCTTCTGCACTn(x)AGTGCAGAAGACGACa
aCAGCAGAAGACGTGAn(x)TCACGTCTTCTGCTGt
    15+[x]+15
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA034C tGTCGTCTTCTGCACTn(x)AGTGCAGAAGACGACa
aCAGCAGAAGACGTGAn(x)TCACGTCTTCTGCTGt
    15+[x]+15
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA034D tGTCGTCTTCTGCACTn(x)AGTGCAGAAGACGACa
aCAGCAGAAGACGTGAn(x)TCACGTCTTCTGCTGt
    15+[x]+15
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA035 tAGTCAGCACCAGGCATn(x)ATGCCTGGTGCTGACTa
aTCAGTCGTGGTCCGTAn(x)TACGGACCACGACTGAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA035B tAGTCAGCACCAGGCATn(x)ATGCCTGGTGCTGACTa
aTCAGTCGTGGTCCGTAn(x)TACGGACCACGACTGAt
    16+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA035C tAGTCAGCACCAGGCATn(x)ATGCCTGGTGCTGACTa
aTCAGTCGTGGTCCGTAn(x)TACGGACCACGACTGAt
    16+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R Low
TNA035D tAGTCAGCACCAGGCATn(x)ATGCCTGGTGCTGACTa
aTCAGTCGTGGTCCGTAn(x)TACGGACCACGACTGAt
    16+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA036 tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA036-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R No
TNA036B tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R Low
TNA036B-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R
TNA036C tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA036C-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R
TNA036D tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA036D-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R
TNA036E tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA036E-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R
TNA036F tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA036F-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R Low
TNA037 tCCATTCCTATGACTGTn(x)AGCTATTGTGTGAGTATa
aGGTAAGGATACTGACAn(x)TCGATAACACACTCATAt
    16+[x]+17
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA037B tCCATTCCTATGACTGTn(x)AGCTATTGTGTGAGTATa
aGGTAAGGATACTGACAn(x)TCGATAACACACTCATAt
    16+[x]+17
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA038 tCCCTCGCTTTCTGATn(x)ATCGGGCGCTGTCGGGa
aGGGAGCGAAAGACTAn(x)TAGCCCGCGACAGCCCt
    15+[x]+16
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA038B tCCCTCGCTTTCTGATn(x)ATCGGGCGCTGTCGGGa
aGGGAGCGAAAGACTAn(x)TAGCCCGCGACAGCCCt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA039 tCCTTTTAAGAAGTTGGCATn(x)ATAATACAATTTAAAACCTGGGTCTCTa
aGGAAAATTCTTCAACCGTAn(x)TATTATGTTAAATTTTGGACCCAGAGAt
    19+[x]+27
(artificial) (std.) +231 WT GG(V&B) 23028976 R Low
TNA039B tCCTTTTAAGAAGTTGGCATn(x)ATAATACAATTTAAAACCTGGGTCTCTa
aGGAAAATTCTTCAACCGTAn(x)TATTATGTTAAATTTTGGACCCAGAGAt
    19+[x]+27
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA040 tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA040-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R No
TNA040B tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA040B-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R No
TNA040C tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA040C-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R
TNA040D tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA040D-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R No
TNA040E tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA040E-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R No
TNA041 tGCTGACGACTTTGTCCCTn(x)ACTCGGTGGGCTTGTTGGCGGa
aCGACTGCTGAAACAGGGAn(x)TGAGCCACCCGAACAACCGCCt
    18+[x]+21
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA041B tGCTGACGACTTTGTCCCTn(x)ACTCGGTGGGCTTGTTGGCGGa
aCGACTGCTGAAACAGGGAn(x)TGAGCCACCCGAACAACCGCCt
    18+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA042 tCTTTACAAGGAGCACGTn(x)ATTAAAGGAGTTTAGCGTGACa
aGAAATGTTCCTCGTGCAn(x)TAATTTCCTCAAATCGCACTGt
    17+[x]+21
(artificial) (std.) +231 WT GG(V&B) 23028976 R Low
TNA042B tCTTTACAAGGAGCACGTn(x)ATTAAAGGAGTTTAGCGTGACa
aGAAATGTTCCTCGTGCAn(x)TAATTTCCTCAAATCGCACTGt
    17+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA043 tCTACCTTCATTGATGTCCCTn(x)ATGAAAGGCAAAGCTCCTCTa
aGATGGAAGTAACTACAGGGAn(x)TACTTTCCGTTTCGAGGAGAt
    20+[x]+20
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA043B tCTACCTTCATTGATGTCCCTn(x)ATGAAAGGCAAAGCTCCTCTa
aGATGGAAGTAACTACAGGGAn(x)TACTTTCCGTTTCGAGGAGAt
    20+[x]+20
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA044 tAGCTCAGTCATCAAAGGCAAAGCTn(x)ACTGGCTCTGGAGGTTACa
aTCGAGTCAGTAGTTTCCGTTTCGAn(x)TGACCGAGACCTCCAATGt
    24+[x]+18
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA044B tAGCTCAGTCATCAAAGGCAAAGCTn(x)ACTGGCTCTGGAGGTTACa
aTCGAGTCAGTAGTTTCCGTTTCGAn(x)TGACCGAGACCTCCAATGt
    24+[x]+18
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA045 tATCCAAAAGAGCGGTCTGATn(x)ATCCTCAGGATTGTTGCa
aTAGGTTTTCTCGCCAGACTAn(x)TAGGAGTCCTAACAACGt
    20+[x]+17
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA045B tATCCAAAAGAGCGGTCTGATn(x)ATCCTCAGGATTGTTGCa
aTAGGTTTTCTCGCCAGACTAn(x)TAGGAGTCCTAACAACGt
    20+[x]+17
(artificial) (NK) +231 WT GG(V&B) 23028976 R Low
TNA046 tGGCGAAAGAGTCCACCACCATn(x)AAGTTCAAGCCATGTCGTa
aCCGCTTTCTCAGGTGGTGGTAn(x)TTCAAGTTCGGTACAGCAt
    21+[x]+18
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA046B tGGCGAAAGAGTCCACCACCATn(x)AAGTTCAAGCCATGTCGTa
aCCGCTTTCTCAGGTGGTGGTAn(x)TTCAAGTTCGGTACAGCAt
    21+[x]+18
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA047 tGCTTGCTTACCTGACCTATn(x)ACATATTTTTGTTTTCAGa
aCGAACGAATGGACTGGATAn(x)TGTATAAAAACAAAAGTCt
    19+[x]+18
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA047B tGCTTGCTTACCTGACCTATn(x)ACATATTTTTGTTTTCAGa
aCGAACGAATGGACTGGATAn(x)TGTATAAAAACAAAAGTCt
    19+[x]+18
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA048 tCGGCCTGCCGGCCCTGGCCGGTn(x)AGGTGGCGGCCGGGCCCTGAGGCGCGGGa
aGCCGGACGGCCGGGACCGGCCAn(x)TCCACCGCCGGCCCGGGACTCCGCGCCCt
    22+[x]+28
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA048B tCGGCCTGCCGGCCCTGGCCGGTn(x)AGGTGGCGGCCGGGCCCTGAGGCGCGGGa
aGCCGGACGGCCGGGACCGGCCAn(x)TCCACCGCCGGCCCGGGACTCCGCGCCCt
    22+[x]+28
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TN0208 tGTATCTGGCCACTGATgacccttctttgttaAAGGAGGCAAAGACAAa
aCATAGACCGGTGACTActgggaagaaacaatTTCCTCCGTTTCTGTTt
    16+[15]+16
hamster, Fut8 G (std.) +63 WT (N/A) NHEJ-ODC 23042119 R
TN5001 tCCCAAAGATCTTCTTTggcactcggactcataagcAGATCACACAGATTGCTa
aGGGTTTCTAGAAGAAAccgtgagcctgagtattcgTCTAGTGTGTCTAACGAt
    16+[19]+17
zebrafish, brip1 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5002 tCCCAAAGATCTTCTTTggcactcggactcataAGCAGATCACACAGa
aGGGTTTCTAGAAGAAAccgtgagcctgagtatTCGTCTAGTGTGTCt
    16+[16]+14
zebrafish, brip1 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5003 tCTCCCACCATCTGCATggcgtacatcatgaaaacccAGCGGTTGCGTTTGGa
aGAGGGTGGTAGACGTAccgcatgtagtacttttgggTCGCCAACGCAAACCt
    16+[20]+15
zebrafish, dusp5 G C (std.) +63 Sharkey-AS+R UA NHEJ Zhang Group Low
TN5004 tCGTCTCTCCGCTCCAGTccagcgcctggacccccAGCGCCGGGCAATa
aGCAGAGAGGCGAGGTCAggtcgcggacctgggggTCGCGGCCCGTTAt
    17+[17]+13
zebrafish, sema3fb G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5005 tCCTCTGCCTGTCTacaagcgcagactgATGATTGAGCTGGCGa
aGGAGACGGACAGAtgttcgcgtctgacTACTAACTCGACCGCt
    13+[14]+15
zebrafish, pax4 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5006 tGCGATCTCTTAATGACTcaggcagctaaagcaAGTCTGGGAGGCCAGAGa
aCGCTAGAGAATTACTGAgtccgtcgatttcgtTCAGACCCTCCGGTCTCt
    17+[15]+17
zebrafish, fev G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5007 tACATTAAATATCACCTctagggttttactattATTTAAAACTGTATGTa
aTGTAATTTATAGTGGAgatcccaaaatgataaTAAATTTTGACATACAt
    16+[16]+16
zebrafish, dre-mir-126, flanking G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5008 tGGCTCGCTCAATTCATggatggagcccgcAATGAGATAGTATGCa
aCCGAGCGAGTTAAGTAcctacctcgggcgTTACTCTATCATACGt
    16+[13]+15
zebrafish, smo G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5009 tGCCCTTCCTGGTTCAgagaacggttgcgcgGCAGATCAGCCTGGTGGa
aCGGGAAGGACCAAGTctcttgccaacgcgcCGTCTAGTCGGACCACCt
    15+[15]+17
zebrafish, acvr1l G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5010 tAGCCGTGAAGATCTTTtcctctagagatgagAAGTCATGGTTTCGAGa
aTCGGCACTTCTAGAAAaggagatctctactcTTCAGTACCAAAGCTCt
    16+[15]+16
zebrafish, acvr1l G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5011 tCCAGCGAGTCGTCTCTggaggcgtacgcccgctgtcTGCGCATGGGCTGCAGa
aGGTCGCTCAGCAGAGAcctccgcatgcgggcgacagACGCGTACCCGACGTCt
    16+[20]+16
zebrafish, plcg1 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5012 tATCACGGTCACACCCTcaccaccaagatcaagttcAGTGATGTGCTCAACa
aTAGTGCCAGTGTGGGAgtggtggttctagttcaagTCACTACACGAGTTGt
    16+[19]+15
zebrafish, plcg1 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5013 tGCAGGCAGTACATCTTcattaacggaacacAGACGGGCGTGAGGGa
aCGTCCGTCATGTAGAAgtaattgccttgtgTCTGCCCGCACTCCCt
    16+[14]+15
zebrafish, slc22a17 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5014 tCGCCACACATCCTGAGTtcagcaagaagatccAGAGCTTAAAAGCAGCCa
aGCGGTGTGTAGGACTCAagtcgttcttctaggTCTCGAATTTTCGTCGGt
    17+[15]+17
zebrafish, G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5015 tAACGCTAATGCTCCTaaagcatctgatgccaagaccAGCGAGGCTGCCAAAGCa
aTTGCGATTACGAGGAtttcgtagactacggttctggTCGCTCCGACGGTTTCGt
    15+[21]+17
zebrafish, basp1 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5016 tCAATGGCAAGCAGTTccctgtgaccagaggccaAGATGTTGGCAAATa
aGTTACCGTTCGTCAAgggacactggtctccggtTCTACAACCGTTTAt
    15+[18]+14
zebrafish, ssr4 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5017 tTCCGAGTCTACAAATcagtcctgcaggcAGTGCAGAAAACTGa
aAGGCTCAGATGTTTAgtcaggacgtccgTCACGTCTTTTGACt
    15+[13]+14
zebrafish, rtn1a G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5018 tCCTCCAACTGCGGCCCCTgcctcatcccactggAGGAGGATCAAATGGGa
aGGAGGTTGACGCCGGGGAcggagtagggtgaccTCCTCCTAGTTTACCCt
    18+[15]+16
zebrafish, npdc1 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5019 tCTGGCAGCAAACCTgcggctgacgtccgATGGTTCAAAAATGa
aGACCGTCGTTTGGAcgccgactgcaggcTACCAAGTTTTTACt
    14+[14]+14
zebrafish, cadm2a G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5020 tAACCGGATTGAACTggtcagagccacgtcgcAGGAGTTGACGATCAGCa
aTTGGCCTAACTTGAccagtctcggtgcagcgTCCTCAACTGCTAGTCGt
    14+[17]+17
zebrafish, cadm2a G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5021 tCCATGCCCATTACCCTgagctttgcactgAAACCAGGTCTGTGGa
aGGTACGGGTAATGGGActcgaaacgtgacTTTGGTCCAGACACCt
    16+[13]+15
zebrafish, dusp5 G C (std.) +63 Sharkey-AS+R UA NHEJ Zhang Group Low
TN5022 tATAGGGATGCGCTACTggcagccggagcggcttcACACATTGGTGGAAGTGa
aTATCCCTACGCGATGAccgtcggcctcgccgaagTGTGTAACCACCTTCACt
    16+[18]+17
zebrafish, adprhl1 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5023 tGCTAAAAGAGAAGCTCTggagcaagcgctaagAAATAAGCTGATGGCGGa
aCGATTTTCTCTTCGAGAcctcgttcgcgattcTTTATTCGACTACCGCCt
    17+[15]+17
zebrafish, amotl2b G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5024 tGCCAAAAAAGTCTATCTtctcgtttattaatgacgACTTCATTGAAAGGAGa
aCGGTTTTTTCAGATAGAagagcaaataattactgcTGAAGTAACTTTCCTCt
    17+[18]+16
zebrafish, snx11 G C (std.) +63 Sharkey-AS+R UA NHEJ Zhang Group Low
TNT01 tGGTAATTTCATCCGTCTgatcgagccatggaacAGGACGCACTTATATa
aCCATTAAAGTAGGCAGActagctcggtaccttgTCCTGCGTGAATATAt
    17+[16]+15
zebrafish, sema3fb, exon 4 G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT02 tGTCCTTTTAACACATaaagatgatatcatACACAGTATTTATTGTa
aCAGGAAAATTGTGTAtttctactatagtaTGTGTCATAAATAACAt
    15+[14]+16
zebrafish, sema3fb, exon 18 G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT03 tATAGTTGAAACCTGATtaacttagtaaaatAACTGCATGGTGGTGCa
aTATCAACTTTGGACTAattgaatcattttaTTGACGTACCACCACGt
    16+[14]+16
zebrafish, dre-mir-126a, 5'-upstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT04 tGGACCCACTGGCATCTgacttgtttaattttAGAGGTTAAAGGAAATa
aCCTGGGTGACCGTAGActgaacaaattaaaaTCTCCAATTTCCTTTAt
    16+[15]+16
zebrafish, dre-mir-126a, 3'-downstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT05 tGGACCCACTGGCATCTgacttgtttaattttAGAGGTTAAAGGaaata
aCCTGGGTGACCGTAGActgaacaaattaaaaTCTCCAATTTCCtttat
    16+[15]+12
zebrafish, dre-mir-126a, 3'-downstream G C (std.) +63 Sharkey-AS+R UA NHEJ T1
TNT06 tATCTGCGTGGACAAACTcaagacaggtttggatgcAGATCAGTGTTATCGa
aTAGACGCACCTGTTTGAgttctgtccaaacctacgTCTAGTCACAATAGCt
    17+[18]+15
zebrafish, dre-mir-126a, 3'-downstream G C (std.) +63 Sharkey-AS+R UA NHEJ T1
TNT07 tACAGAATCTGCATTGTataagcgcaatcgaattgATTGTGGGTGTGTTATa
aTGTCTTAGACGTAACAtattcgcgttagcttaacTAACACCCACACAATAt
    16+[18]+16
zebrafish, dre-mir-126b, 5'-upstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT08 tGACTGGTGAACACAACTataacctacagactgAGCACGTCCGACTGCATa
aCTGACCACTTGTGTTGAtattggatgtctgacTCGTGCAGGCTGACGTAt
    17+[15]+17
zebrafish, dre-mir-126b, 3'-downstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT09 tATCATGAGCTCGTCGCTcgttgacgtaaatgcATTGTTGATTTCATa
aTAGTACTCGAGCAGCGAgcaactgcatttacgTAACAACTAAAGTAt
    17+[15]+14
zebrafish, dre-mir-17a-1 [SNP], 5'-upstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT10 tGCACTTGTCCCGGCCTgtaaaggattgtggagATTGTACCCCACAAATa
aCGTGAACAGGGCCGGAcatttcctaacacctcTAACATGGGGTGTTTAt
    16+[16]+16
zebrafish, dre-mir-92a-1, 3'-downstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT11 tAGTCCTCTCCACCTccatagttggggagaATGTGGGAGATTTGa
aTCAGGAGAGGTGGAggtatcaacccctctTACACCCTCTAAACt
    14+[15]+14
zebrafish, dre-mir-17a-1, 5'-upstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT12 tATCCACTTAATTACTGTgtattagatctgatATCAACTGAACAGGGTa
aTAGGTGAATTAATGACAcataatctagactaTAGTTGACTTGTCCCAt
    17+[14]+16
zebrafish, dre-mir-92a-1, 3'-downstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT13 tGTCCCACGTCATGTcactgtagtgtcaaAGTGCTTACAGTGCa
aCAGGGTGCAGTACAgtgacatcacagttTCACGAATGTCACGt
    14+[14]+14
zebrafish, dre-mir-17a-2, 5'-upstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT14 tCTAACATCCTTACAGGTgtgttgaggcaagttagAGCTAAAGTCTGCTGGa
aGATTGTAGGAATGTCCAcacaactccgttcaatcTCGATTTCAGACGACCt
    17+[17]+16
zebrafish, dre-mir-92a-2, 3'-downstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT15 tGAGCCCTACTCTGTCATtgaggtggtgccaagtgATTTGATGTGCACTGCTGa
aCTCGGGATGAGACAGTAactccaccacggttcacTAAACTACACGTGACGACt
    17+[17]+18
zebrafish, fgd5 G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT16 tACATCTCCAACTATGATcgcagcatgtcagtgctggACGAGAGCTGTAGAAAGa
aTGTAGAGGTTGATACTAgcgtcgtacagtcacgaccTGCTCTCGACATCTTTCt
    17+[19]+17
zebrafish, G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT17 tGCAGCTACACCTTCATcctgcccgagatggagAGCTGTCAGGAGCAGa
aCGTCGATGTGGAAGTAggacgggctctacctcTCGACAGTCCTCGTCt
    16+[16]+15
zebrafish, [unnamed] C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT18 tCTCAGGATGCTCTGATTtgttttgtacactgggaAATAGTGAAAAGTGGCTa
aGAGTCCTACGAGACTAAacaaaacatgtgaccctTTATCACTTTTCACCGAt
    17+[17]+17
zebrafish, psmf1 G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT19 tTTACATTCATCCAGACtcgcctgcttccggtgaAACGTGGATGCGCCAa
aAATGTAAGTAGGTCTGagcggacgaaggccactTTGCACCTACGCGGTt
    16+[17]+15
zebrafish, tbx18 G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT20 tCTTCTGAAATTCGGTtccggtaaagacccggggaCATCAAACGAGAGCa
aGAAGACTTTAAGCCAaggccatttctgggcccctGTAGTTTGCTCTCGt
    15+[19]+14
zebrafish, tcf21 G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT21 tCACACCGGAGCGCCAtacggctcgctggcccaGCACTCCTTCATCAa
aGTGTGGCCTCGCGGTatgccgagcgaccgggtCGTGAGGAAGTAGTt
    15+[17]+14
zebrafish, wt1a G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT22 tGGCCTTACACTATCCATgacctcaggaaccttggAGCCAAGAAGTTTTATGGa
aCCGGAATGTGATAGGTActggagtccttggaaccTCGGTTCTTCAAAATACCt
    17+[17]+18
zebrafish, mical2b G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT23 tGTCCCGCAGTGCACTgacggagattgttgaccAGGAGGCAGCTTATCTGGa
aCAGGGCGTCACGTGActgcctctaacaactggTCCTCCGTCGAATAGACCt
    15+[17]+18
zebrafish, gtf3c3 G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT24 tCCACCTGCACCTCAGGTcctgcagagatcaaactccATTCAGCAGATGGAGCa
aGGTGGACGTGGAGTCCAggacgtctctagtttgaggTAAGTCGTCTACCTCGt
    17+[19]+16
zebrafish, [unnamed] C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT25 tCTAAGCTCTCCCTGTccattctagacgcctgAGTTCAGGAAAACTTCa
aGATTCGAGAGGGACAggtaagatctgcggacTCAAGTCCTTTTGAAGt
    15+[16]+16
zebrafish, midn G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT26 tCGCATGCATTAGTgaaaataggcctactacacATATAAAAGGCGCGCCa
aGCGTACGTAATCActtttatccggatgatgtgTATATTTTCCGCGCGGt
    13+[19]+16
(Tol2), [5'-arm] (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT27 tACAACTACAACAGCCACAACGTCTatatcatggccgacaAGCAGAAGAACGGCa
aTGTTGATGTTGTCGGTGTTGCAGAtatagtaccggctgtTCGTCTTCTTGCCGt
    24+[15]+14
eGFP G (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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