EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
TALEN targeting sequences from pig (Sus scrofa): 28 TALEN records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0171 tATACATTTTACACACATATatatgaaactgacagtATTAATGGCCTGAACCTa
aTATGTAAAATGTGTGTATAtatactttgactgtcaTAATTACCGGACTTGGAt
    19+[16]+17
pig, SRY, 3'-downstream 1.4kb G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0172 tATATGAAACTGACAGTATtaatggcctgaacctAGCCAGAACTCAGATTGGGa
aTATACTTTGACTGTCATAattaccggacttggaTCGGTCTTGAGTCTAACCCt
    18+[15]+19
pig, SRY, 3'-downstream 1.5kb G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0173 tGCCCCCCCACACAGCTgagcccatgctgatggAGTACCCTGAGGCTATa
aCGGGGGGGTGTGTCGActcgggtacgactaccTCATGGGACTCCGATAt
    16+[16]+16
pig, RELA G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0174 tCCCACTCCCCTGGGGGCCTCTg...cCTCTCGGGGGACGAAGa
aGGGTGAGGGGACCCCCGGAGAc...gGAGAGCCCCCTGCTTCt
    21+[18]+16
pig, RELA G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R Low
TN0175 tCACCAACGGTCTCCTCTCGGGggacgaagacttctccTCCATTGCGGACATGGa
aGTGGTTGCCAGAGGAGAGCCCcctgcttctgaagaggAGGTAACGCCTGTACCt
    21+[16]+16
pig, RELA G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R Low
TN0176 tGGAACATGCATTCAACATcgccaagtatcagttAGGCATAGAGAAACTACTGGa
aCCTTGTACGTAAGTTGTAgcggttcatagtcaaTCCGTATCTCTTTGATGACCt
    18+[15]+20
pig, DMD G (std.) +63-Goldy, +231 WT GG(V&B) NHEJ, NHEJ-DisDel, NHEJ-Inv 23027955 R
TN0177 tCTATACCTAGGTCAAAAATg...cATGGCTGGATTGCAACAAACTa
aGATATGGATCCAGTTTTTAc...gTACCGACCTAACGTTGTTTGAt
    19+[15]+21
pig, DMD, intron-exon G (std.) +63-Goldy WT GG(V&B) NHEJ, NHEJ-DisDel, NHEJ-Inv 23027955 R
TN0178 tCGCCAAGTATCAGTTAGGCATa...aAGGTTAGTGCATTTTGGGa
aGCGGTTCATAGTCAATCCGTAt...tTCCAATCACGTAAAACCCt
    21+[21]+18
pig, DMD, exon-intron G (std.) +63-Goldy, +231 WT GG(V&B) 23027955 R No
TN0179 tCCTTCCAAGGCTTCTACATc...cACTTTTGATAAGACATTCTTGGTa
aGGAAGGTTCCGAAGATGTAg...gTGAAAACTATTCTGTAAGAACCAt
    19+[16]+23
pig, AMELY, Intron G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0180 tCTACATCAATCACTTGGCTttcacttttgataagACATTCTTGGTATGGATa
aGATGTAGTTAGTGAACCGAaagtgaaaactattcTGTAAGAACCATACCTAt
    19+[15]+17
pig, AMELY, Intron G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R No
TN0182 tCTGCAAATACATACTTCATg...cATCTTTTACATCATGGTGGa
aGACGTTTATGTATGAAGTAc...gTAGAAAATGTAGTACCACCt
    19+[16]+19
pig, GGTA1 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0183 tGCGTTCCTTTAAAGTGTttgagatcaagtccgAGAAGAGGTGGCAAGACa
aCGCAAGGAAATTTCACAaactctagttcaggcTCTTCTCCACCGTTCTGt
    17+[15]+17
pig, GGTA1 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0186 tCCAGTGCAACAGCTCCACCTg...gGGCCTGTGATGGTGATCCTGa
aGGTCACGTTGTCGAGGTGGAc...cCCGGACACTACCACTAGGACt
    20+[16]+20
pig, LDLR G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0187 tCTCCTACAAGTGGATTTgtgatgggaacaccgAGTGCAAGGACGGGTCCGa
aGAGGATGTTCACCTAAAcactacccttgtggcTCACGTTCCTGCCCAGGCt
    17+[15]+18
pig, LDLR G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0188 tCCACTCCAGCTGGCGCTgcgacggagacactgACTGCAAGGACAAGTCTGACGa
aGGTGAGGTCGACCGCGAcgctgcctctgtgacTGACGTTCCTGTTCAGACTGCt
    17+[15]+21
pig, LDLR G (std.) +63-Goldy, +231 WT GG(V&B) 23027955 R No
TN0189 tGCAACAGCTCCACCTGCATCCCTg...gATGGTGATCCTGACTGCGAGGa
aCGTTGTCGAGGTGGACGTAGGGAc...cTACCACTAGGACTGACGCTCCt
    23+[16]+21
pig, LDLR G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0190 tACCTTCCTCCTCTCCGCTacccagccacttgcacATTCAAAAGCAGCTTAGa
aTGGAAGGAGGAGAGGCGAtgggtcggtgaacgtgTAAGTTTTCGTCGAATCt
    18+[16]+17
pig, RAG2 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0191 tGGTAATAACATGGCCTTAATt...cATTGATGAATTTTGATGGGCa
aCCATTATTGTACCGGAATTAa...gTAACTACTTAAAACTACCCGt
    20+[15]+20
pig, RAG2 G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0192 tCCCAAAGGTTCAGTGTTTtgtgttcaatgttgAGTACATGAATTGCACTTGGa
aGGGTTTCCAAGTCACAAAacacaagttacaacTCATGTACTTAACGTGAACCt
    18+[14]+20
pig, IL2RG G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0193 tCTACCAAACATTTGTTGTccagctccaggacccACGGGAACCCAGGAGGCAGGa
aGATGGTTTGTAAACAACAggtcgaggtcctgggTGCCCTTGGGTCCTCCGTCCt
    18+[15]+20
pig, IL2RG G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R No
TN0194 tCCCCTGCCCGGCTTTCTTCTctcacttcagctctgAGCCAGGTGAGGGGGa
aGGGGACGGGCCGAAAGAAGAgagtgaagtcgagacTCGGTCCACTCCCCCt
    20+[15]+15
pig, GHRHR, intron-exon G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0195 tCCCATCTCGCCGTCCAGGCTg...gATGGGCTGTTGTGCTGGCCGa
aGGGTAGAGCGGCAGGTCCGAc...cTACCCGACAACACGACCGGCt
    20+[15]+20
pig, GHRHR, exon-intron G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0196 tCCCTTTTTAGAAGTCAAGGTa...aAAGATCCAGGAGAGATTTTGGa
aGGGAAAAATCTTCAGTTCCAt...tTTCTAGGTCCTCTCTAAAACCt
    20+[15]+21
pig, MSTN G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0197 tGGATTATTGCACCCAAAAGATa...gCTCTGGAGAGTGTGAATTTGTa
aCCTAATAACGTGGGTTTTCTAt...cGAGACCTCTCACACTTAAACAt
    21+[16]+21
pig, MSTN G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0198 tCTAACTGTGGATTTTGAAGCTtttggatgggactggATTATTGCACCCAAAAGa
aGATTGACACCTAAAACTTCGAaaacctaccctgaccTAATAACGTGGGTTTTCt
    21+[15]+17
pig, MSTN G (std.) +63-Goldy, +231 WT GG(V&B) NHEJ 23027955 R Low
TN0202 tGTTGAGTGACAATTTCTCCCTCCTGCAGGTt...gATGAGGGTTCTGTGGGTTGCTTTGGTGGTa
aCAACTCACTGTTAAAGAGGGAGGACGTCCAa...cTACTCCCAAGACACCCAACGAAACCACCAt
    30+[21]+29
pig, APOE, intron-exon G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0203 tGTTCCCCAGGCCTGGTTCATACTCTt...tACCAGGAGGTGGGAGCCTTGGGTACCa
aCAAGGGGTCCGGACCAAGTATGAGAa...aTGGTCCTCCACCCTCGGAACCCATGGt
    25+[18]+26
pig, HR G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0204 tGGATGAAACCGAAATTagaagtttctttgctagATATGGTTCAGTAAAAGa
aCCTACTTTGGCTTTAAtcttcaaagaaacgatcTATACCAAGTCATTTTCt
    16+[17]+17
pig, DAZL G (std.) +63-Goldy WT GG(V&B) 23027955 R No




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
GSearch EENdb with this gene symbol
RSearch EENdb with this reference

©2012-2013  PKU Zebrafish Functional Genomics Group
School of Life Sciences, Peking University, Beijing, China
Reference of EENdb    Contact us