EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
ZFN targeting sequences from tobacco (Nicotiana tabacum): 6 ZFN records match.
(Click on ID to see details)


ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0028 gGAATCCTTCtaatgaGGCGGAGATc
cCTTAGGAAGattactCCGCCTCTAg
    3F+[6]+3F
tobacco, SuRB/SuRA G ZP10335 ZP10337 LRGS WT OPEN NHEJ 18657511 R
ZN0031 gCTCCTGGTCGGAaatattTCGGACGAGGCCc
cGAGGACCAGCCTttataaAGCCTGCTCCGGg
    4F+[6]+4F
tobacco, CHN50 G ZP10343 ZP10344 LRGS WT Sangamo HR 19112554 R
ZN0037 cTACCACGTCacgagcAGGGTGGTGt
gATGGTGCAGtgctcgTCCCACCACa
    3F+[6]+3F
tobacco, SuRB G ZP10392 ZP10393 LRGS WT, D+R MA NHEJ; HR 19404258 R
ZN0037-T002 cTGCCACGTCacgagcAGGGCGGTGt
gACGGTGCAGtgctcgTCCCGCCACa
    3F+[6]+3F
tobacco, SuRA G ZP10392-T002 ZP10393-T002 LRGS WT, D+R MA NHEJ; HR 19404258 R
ZN0038 gGGCAGCACCagatgTGGGCTGCTc
cCCGTCGTGGtctacACCCGACGAg
    3F+[5]+3F
tobacco, SuRB G ZP10400 ZP10406 LRGS EL+KK OPEN NHEJ; HR 19404258 R
ZN0038-T002 gGGCAACACCagatgTGGGCTGCTc
cCCGTTGTGGtctacACCCGACGAg
    3F+[5]+3F
tobacco, SuRA G ZP10400-T002 ZP10406 LRGS EL+KK OPEN NHEJ; HR 19404258 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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