EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
ZFN targeting sequences from human (Homo sapiens): 96 ZFN records match.
(Click on ID to see details)


ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0003 tCGGAGCCGCTTTaacccACTCTGTGGAAGt
aGCCTCGGCGAAAttgggTGAGACACCTTCa
    4F+[5]+4F
human, IL2RG G C ZP10005 ZP10006 (N/A) WT Sangamo HR 15806097 R
ZN0006 tGTCCCCTTCtgggctcactatGCTGCCGCCc
aCAGGGGAAGacccgagtgataCGACGGCGGg
    3F+[12]+3F
human, CCR5 G C ZP10013 ZP10014 TG(G4S)3 WT MA 16084494 R
ZN0007 gGCCCGCCCCctagcgGCCGGGGAGg
cCGGGCGGGGgatcgcCGGCCCCTCc
    3F+[6]+3F
human, DMPK, 3'-UTR G C ZP10012 ZP10011 TG WT MA 16084494 R
ZN0009 cGGCGCCCACcatcgaGTCGCAGCCc
gCCGCGGGTGgtagctCAGCGTCGGg
    3F+[6]+3F
human, CD8A G C ZP10020 ZP10021 (3aa) WT MA HR 16169774 R
ZN0018 cCTACTCaTTCAACcaataGCCCGGGCCGTAc
gGATGAGtAAGTTGgttatCGGGCCCGGCATg
    (2F+1+2F)+[5]+4F
human, (mtDNA) G C ZP10051, ZP10052; ZP10053 - ZP10057 ZP10061, ZP10062; ZP10063 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018-T002 cCTACTCaTTCAACcaataGCCCTGGCCGTAc
gGATGAGtAAGTTGgttatCGGGACCGGCATg
    (2F+1+2F)+[5]+4F
human, (mtDNA) G C ZP10053 - ZP10057 ZP10061-T002 - ZP10063-T002 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018B cAGCCTACTCaTTCAACcaataGCCCGGGCCGTAc
gTCGGATGAGtAAGTTGgttatCGGGCCCGGCATg
    (2F+1+3F)+[5]+4F
human, (mtDNA) G C ZP10058 ZP10061 - ZP10063 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018B-T002 cAGCCTACTCaTTCAACcaataGCCCTGGCCGTAc
gTCGGATGAGtAAGTTGgttatCGGGACCGGCATg
    (2F+1+3F)+[5]+4F
human, (mtDNA) G C ZP10058 ZP10061-T002 - ZP10063-T002 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018C
(Z-S1)
GCCCGGGCCGTAc
CGGGCCCGGCATg
    4F
human, (mtDNA) [mutated] G C ZP10063 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZNU017
(Z-S1)
(N/A)
    
human, (mtDNA) G C S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0019 gGTCATCCTCATCctgatAAACTGCAAAAGg
cCAGTAGGAGTAGgactaTTTGACGTTTTCc
    4F+[5]+4F
human, CCR5 G C ZP10064 ZP10065 LRGS WT, EL+KK Sangamo NHEJ 18587387 R
ZN0019-T002 gGTCGTCCTCATCttaatAAACTGCAAAAAg
cCAGCAGGAGTAGaattaTTTGACGTTTTTc
    4F+[5]+4F
human, CCR2 G C ZP10064-T002 ZP10065-T002 LRGS EL+KK Sangamo NHEJ 18587387 R
ZN0019-T003 aGTCATCCTCATCtcacgGATGAGGATGCCa
tCAGTAGGAGTAGagtgcCTACTCCTACGGt
    4F+[5]+4F
human, ABLIM2, intron G C ZP10064 ZP10064-T003 LRGS EL+KK Sangamo NHEJ 18587387 R Low
ZN0022 tCACTTCTAAtggtgaTTATGGGAGa
aGTGAAGATTaccactAATACCCTCt
    3F+[6]+3F
human, CFTR, intron G C ZP10153 ZP10165 LRGS WT OPEN NHEJ 18657511 R
ZN0023 cAGCGTCTTCgagagtGAGGACGTGt
gTCGCAGAAGctctcaCTCCTGCACa
    3F+[6]+3F
human, VEGFA, 5'-up G C ZP10176, ZP10181 ZP10186, ZP10190 LRGS WT, EL+KK OPEN NHEJ; HR 18657511 R
ZN0024 gAGCAGCGTCttcgaGAGTGAGGAc
cTCGTCGCAGaagctCTCACTCCTg
    3F+[5]+3F
human, VEGFA, 5'-up G C ZP10213, ZP10216 ZP10224, ZP10227 LRGS WT, EL+KK OPEN NHEJ; HR 18657511 R
ZN0025 cCCCAGCCGCcccactGAGTTTGCCt
gGGGTCGGCGgggtgaCTCAAACGGa
    3F+[6]+3F
human, HOXB13 G C ZP10280, ZP10282 ZP10284, ZP10285 LRGS WT OPEN NHEJ 18657511 R
ZN0026 aTGCCACCCAtgcccTGGGGTGCCc
tACGGTGGGTacgggACCCCACGGg
    3F+[5]+3F
human, HOXB13 G C ZP10297, ZP10299 ZP10302, ZP10306 LRGS WT OPEN NHEJ 18657511 R
ZN0027 gAGACTCCCAcggccGGGGAAGAGt
cTCTGAGGGTgccggCCCCTTCTCa
    3F+[5]+3F
human, HOXB13 G C ZP10314, ZP10319 ZP10325, ZP10326 LRGS WT OPEN NHEJ 18657511 R
ZN0043 aAGTCCAATCTATgacatcAATTATTATACAt
tTCAGGTTAGATActgtagTTAATAATATGTa
    4F+[6]+4F
human, CCR5 G C ZP10438 ZP10439 TGEK WT MA NHEJ 19470664 R
ZN0044 tCTGGGCTCACTAtgctgcCGCCCAGTGGGAc
aGACCCGAGTGATacgacgGCGGGTCACCCTg
    4F+[6]+4F
human, CCR5 G C ZP10440 ZP10441 TGEK WT MA NHEJ 19470664 R
ZN0045 cTTCTTCATCATCctcctGACAATCGAt
gAAGAAGTAGTAGgaggaCTGTTAGCTa
    4F+[5]+3F
human, CCR5/CCR2 G C ZP10442 ZP10443 TGEK WT MA NHEJ 19470664 R
ZN0046 cTGTGTTTGCTTTaaaagcCAGGACGGTCACc
gACACAAACGAAAttttcgGTCCTGCCAGTGg
    4F+[6]+4F
human, CCR5/CCR2 G C ZP10444 ZP10445 TGEK WT MA NHEJ 19470664 R
ZN0047 cAGGACGGTCacctttGGGGTGGTGa
gTCCTGCCAGtggaaaCCCCACCACt
    3F+[6]+3F
human, CCR5 G C ZP10446 ZP10447 TGEK WT MA NHEJ 19470664 R
ZN0048 aCGGTCACCTttggggTGGTGACAAGTGt
tGCCAGTGGAaaccccACCACTGTTCACa
    3F+[6]+4F
human, CCR5/CCR2 G C ZP10448 ZP10449 TGEK WT MA NHEJ 19470664 R
ZN0049 aCCAAGCTATgcaggtGACAGAGACt
tGGTTCGATAcgtccaCTGTCTCTGa
    3F+[6]+3F
human, CCR5 G C ZP10450 ZP10451 TGEK WT MA NHEJ 19470664 R
ZN0050 cCCCATCATCTATgcctttGTCGGGGAGAAGt
gGGGTAGTAGATAcggaaaCAGCCCCTCTTCa
    4F+[6]+4F
human, CCR5 G C ZP10452 ZP10453 TGEK WT, D+R, EL+KK MA NHEJ 19470664 R
ZN0050-T002 cCCCATCATCTATgccttCGTTGGGGAGAAGt
gGGGTAGTAGATAcggaaGCAACCCCTCTTCa
    4F+[5]+4F
human, CCR2 G C ZP10452 ZP10453-T002 TGEK WT, D+R, EL+KK MA NHEJ 19470664 R Low
ZN0051 aGGCTACATCtttgcTTTGTTGGCa
tCCGATGTAGaaacgAAACAACCGt
    3F+[5]+3F
human, PIGA G C ZP10454; ZP10455 ZP10457; ZP10456 LRGS WT OPEN NHEJ; HR 19540188 R
ZN0055 tACTGTCCCCTTCtgggctcactatGCTGCCGCCCAGt
aTGACAGGGGAAGacccgagtgataCGACGGCGGGTCa
    4F+[12]+4F
human, CCR5 G C ZP10465 ZP10464 QNKKGLD(G4S)3 WT, D+R, EL+KK MA NHEJ 19635463 R
ZN0056 aGCTCTCATTTTCcatacaGTCAGTATCAATt
tCGAGAGTAAAAGgtatgtCAGTCATAGTTAa
    4F+[6]+4F
human, CCR5 G C ZP10467 ZP10466 QNKK WT, D+R, EL+KK MA NHEJ 19635463 R
ZN0057 cACCCCACAGTGGggccacTAGGGACAGGATt
gTGGGGTGTCACCccggtgATCCCTGTCCTAa
    4F+[6]+4F
human, PPP1R12C, intron G C ZP10468 ZP10469 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0058 cCAGACCtGGCACCCAGgagagGAGCAGGCAGGGTCAGCTg
gGTCTGGaCCGTGGGTCctctcCTCGTCCGTCCCAGTCGAc
    (3F+1+2F)+[5]+6F
human, POU5F1, intron G C ZP10470 ZP10471 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0059 cTGCAGCTGCCCAGACCTGgcaccCAGGAGaGGAGCAGGCa
gACGTCGACGGGTCTGGACcgtggGTCCTCtCCTCGTCCGt
    6F+[5]+(2F+1+3F)
human, POU5F1, intron G C ZP10472 ZP10473 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0060 aGTAGTCCTTTGTTACatgcatGAGTCAGTGAACAGGg
tCATCAGGAAACAATGtacgtaCTCAGTCACTTGTCCc
    5F+[6]+5F
human, POU5F1, intron G C ZP10474 ZP10475 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0061 cCCCAGCTCCCAGAGCacggctGCAAGGGCCaGGAGCAc
gGGGTCGAGGGTCTCGtgccgaCGTTCCCGGtCCTCGTg
    5F+[6]+(3F+1+2F)
human, PITX3 G C ZP10477 ZP10476 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0061-T002 tAGCTCACaGTCCCTTGT AAAAGGGCCCaGGGAGCAg
aTCGAGTGtCAGGGAACA TTTTCCCGGGtCCCTCGTc
    5F+5F
human, PKP1, intron G C ZP10477-T002 ZP10477-T003 LRGS EL+KK Sangamo NHEJ 19680244 R Low
ZN0065 aGGCTCCTCCTCCaacattGGGGATTATGATt
tCCGAGGAGGAGGttgtaaCCCCTAATACTAa
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10484 ZP10485 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0066 cTCATCCATTTGCactcctGGGTATGGAGACc
gAGTAGGTAAACGtgaggaCCCATACCTCTGg
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10486 ZP10487 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0067 cATACCCACCTCAcgtgtgGTCAATGGAGTGt
gTATGGGTGGAGTgcacacCAGTTACCTCACa
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10488 ZP10489 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0068 gGTCCCATCACCAgcgatgGATATAGTCTGGa
cCAGGGTAGTGGTcgctacCTATATCAGACCt
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10490 ZP10491 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0069 gGTCTGCAGCCACagctggGTGGATGCAGCTg
cCAGACGTCGGTGtcgaccCACCTACGTCGAc
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10492 ZP10493 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0070 tGACCTCACCACCtctcagTGGGAAGAGGAGg
aCTGGAGTGGTGGagagtcACCCTTCTCCTCc
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10494 ZP10495 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0071 tTTCTATGGCAGCctcaggGGAGAATGGGACt
aAAGATACCGTCGgagtccCCTCTTACCCTGa
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10496 ZP10497 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0138B gGGAGTGgTGCATGGTGtggcaaCAGGTAaGCTCCAtTGTGCTt
cCCTCACcACGTACCACaccgttGTCCATtCGAGGTaACACGAa
    (3F+1+2F)+[6]+(2F+1+2F+1+2F)
human, SNCA G C ZP10755, ZP10756 ZP10759 WT, ELD+KKR Sangamo NHEJ; HR 20120421 R
ZNU001 (N/A)
    
human, KDR G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU002 (N/A)
    
human, TP73 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU003 (N/A)
    
human, MAP3K14 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU004 (N/A)
    
human, EP300 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU005 (N/A)
    
human, BTK G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU006 (N/A)
    
human, CARM1 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU007 (N/A)
    
human, GNAI2 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU008 (N/A)
    
human, TSC2 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU009 (N/A)
    
human, RIPK1 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU010 (N/A)
    
human, NR3C1 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZN0084 aGGCCGCTGCccagcaGCAGGAGTCa
tCCGGCGACGggtcgtCGTCCTCAGt
    3F+[6]+3F
human, RHO G C ZP10543 ZP10542 (G3S)4 WT MA NHEJ; HR 20671268 R
ZN0085 tGCTGCTGCTgctgctgctGCTGCTGCTg
aCGACGACGAcgacgacgaCGACGACGAc
    3F+[3n]+3F
human, (microsatellite) G C ZP10545 ZP10544 (N/A) WT MA HR 20676085 R
ZN0112 gATGGGCAGCGCCcgagtGGCGGAGCTGCTg
cTACCCGTCGCGGgctcaCCGCCTCGACGAc
    4F+[5]+4F
human, CDKN2A G C ZP10695 ZP10696 TGEK WT MA(2F) NHEJ 21191366 R
ZN0113 cTCACCCGACCCGtgcacGACGCTGCCCGGg
gAGTGGGCTGGGCacgtgCTGCGACGGGCCc
    4F+[5]+4F
human, CDKN2A G C ZP10697 ZP10698 TGEK WT MA(2F) NHEJ 21191366 R
ZN0116 cCCTCAAgCAGGCCCCGCTGgtgcactGAAGAGcCACCCTGTGg
gGGAGTTcGTCCGGGGCGACcacgtgaCTTCTCgGTGGGACACc
    (4F+1+2F)+[7]+(2F+1+3F)
human, CLTA G C ZP10703 ZP10704 LRGS ELD+KKR Sangamo HR 21297641 R
ZN0117 gGGGGGCCTCaCGCACCcgcggcGCAGGAGCTTCAGTGg
cCCCCCGGAGtGCGTGGgcgccgCGTCCTCGAAGTCACc
    (2F+1+3F)+[6]+5F
human, DNM2 G C ZP10705 ZP10706 LRGS ELD+KKR Sangamo HR 21297641 R
ZN0120 tGACCGCTTCTACcccaatGACTTGTGGGTGg
aCTGGCGAAGATGgggttaCTGAACACCCACc
    4F+[6]+4F
human, CXCR4 G C ZP10715 ZP10716 LRGS EL+KK Sangamo NHEJ 21533216 R
ZN0120-T002 cCACCCACAGGTTttaaggGTAGAAGCAGCAa
gGTGGGTGTCCAAaattccCATCTTCGTCGTt
    4F+[6]+4F
human, ADAMTS17, intron G C ZP10715-T002 ZP10716-T002 LRGS EL+KK Sangamo NHEJ 21533216 R Low
ZN0120-T003 cCACCTACTTGTCttgtgtGTTGAAGCAGACa
gGTGGATGAACAGaacacaCAACTTCGTCTGt
    4F+[6]+4F
human, (intergenic) G C ZP10715-T003 ZP10716-T003 LRGS EL+KK Sangamo NHEJ 21533216 R Low
ZN0122 gGACTCCTTCaccaaggGGAGGGGGAg
cCTGAGGAAGtggttccCCTCCCCCTc
    3F+[7]+3F
human, IDUA, intron-exon G C ZP10718 ZP10719 TGPNRGVTK ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0123 gGCCCCCCCCcgccccGCAGATGAGg
cCGGGGGGGGgcggggCGTCTACTCc
    3F+[6]+3F
human, IDUA, intron-exon G C ZP10720 ZP10721 TGGS / LGGS / LRGS ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0124 aCCCTGCGCCccgcgctGCGGCTGCCg
tGGGACGCGGggcgcgaCGCCGACGGc
    3F+[7]+3F
human, IDUA G C ZP10722 ZP10723 TGPNRGVTK ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0125 gCCCCGCCCCttacccGCCGGCGGCc
cGGGGCGGGGaatgggCGGCCGCCGg
    3F+[6]+3F
human, IDUA G C ZP10724 ZP10725 TGGS / LGGS / LRGS ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0126 gCTCAACCTCgcctatGTGGGCGCCg
cGAGTTGGAGcggataCACCCGCGGc
    3F+[6]+3F
human, IDUA G C ZP10726 ZP10727 TGGS / LGGS / LRGS ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0127 cAACCTCGCCtatgtgGGCGCCGTCc
gTTGGAGCGGatacacCCGCGGCAGg
    3F+[6]+3F
human, IDUA G C ZP10728 ZP10729 TGGS / LGGS / LRGS ELD+KKR CoDA HR 21663559 R Low
ZN0130 tCCCCTGCTTGGCtgggcgCAGTGGCTCATGc
aGGGGACGAACCGacccgcGTCACCGAGTACg
    4F+[6]+4F
human, TP53, intron G C ZP10734 ZP10735 QNKK WT, EL+KK MMA NHEJ; HR 21695267 R
ZN0131 cCACCATCCACTAcaactaCATGTGTAACAGt
gGTGGTAGGTGATgttgatGTACACATTGTCa
    4F+[6]+4F
human, TP53 G C ZP10736 ZP10737 QNKK WT, EL+KK MMA NHEJ; HR 21695267 R
ZN0136 gTGCCAGGTACTGTGTcagggtACTAGGGGTATGg
cACGGTCCATGACACAgtcccaTGATCCCCATACc
    5F+[6]+4F
human, F9, intron G C ZP10751 ZP10752 LRGS WT Sangamo NHEJ; HR 21706032 R
ZN0138 gGGAGTGgTGCATGGTGtggcaaCAGGTAaGCTCCATTGTGCtt
cCCTCACcACGTACCACaccgttGTCCATtCGAGGTAACACGaa
    (3F+1+2F)+[6]+(2F+1+4F)
human, SNCA G C ZP10755, ZP10756 ZP10757, ZP10758; ZP10759 LRGS WT, ELD+KKR Sangamo NHEJ; HR 21757228 R
ZN0139 tGTATACATAAAGCTAcaaacatGGAGTTTGCAGTGAAGGTAa
aCATATGTATTTCGATgtttgtaCCTCAAACGTCACTTCCATt
    5F+[7]+(1+6F)
human, RPS6KA3 G C ZPU0026 ZPU0027 (N/A) (N/A) Sangamo HR; HR-DisDel 21765410 R
ZN0140 tGGCTCCTACTCTGTTtgcaagCGATGCATACATGCAAc
aCCGAGGATGAGACAAacgttcGCTACGTATGTACGTTg
    5F+[6]+(1+5F)
human, RPS6KA6 G C ZPU0028 ZPU0029 (N/A) (N/A) Sangamo HR; HR-DisDel 21765410 R
ZNU011 nNNN[x]n(y)gaGATGCAAGATTGCTCAGGGt
nNNN[x]n(y)ctCTACGTTCTAACGAGTCCCa
    xF+[y+2]+(1+6F)
human, IRAK4 G C (N/A) ZPU0030 (N/A) (N/A) Sangamo HR-DisDel 21765410 R
ZN0143 aTACGCCtCGCCCGAGCtgtgcgGTAGGCATTGAGGCAGCCa
tATGCGGaGCGGGCTCGacacgcCATCCGTAACTCCGTCGGt
    (3F+1+2F)+[6]+6F
human, MALAT1, promoter G C ZP10764 ZP10765 LRGS EL+KK Sangamo NHEJ; HR 21844124 R
ZN0144 aGTCTGCCGTTACtgccctGTGGGGCAAGGTGAACGTGg
tCAGACGGCAATGacgggaCACCCCGTTCCACTTGCACc
    4F+[6]+(1+6F)
human, HBB G C ZPU0031 ZPU0032 (N/A) (N/A) Sangamo HR 21881051 R
ZN0145 cCGTTACTGCcctgtGGGGCAAGGt
gGCAATGACGggacaCCCCGTTCCa
    3F+[5]+3F
human, HBB G C ZP10766; ZP10767 (N/A) EL+KK, ELD+KKR OPEN / CoDA NHEJ; HR 21898685 R
ZN0146 aCCTGACTCCtgtggaGAAGTCTGCc
tGGACTGAGGacacctCTTCAGACGg
    3F+[6]+3F
human, HBB G C ZP10070, ZP10771 (N/A) EL+KK, ELD+KKR OPEN / CoDA NHEJ; HR 21898685 R
ZN0148 cCTGTGTGCCCTGAGCCCGctgggGCAGCTGCTGCAGGAc
gGACACACGGGACTCGGGCgacccCGTCGACGACGTCCTg
    6F+[5]+5F
human, SLC16A3 G C ZPU0039 ZPU0040 (N/A) (N/A) Sangamo NHEJ 21930917 R
ZN0149 cCACGGAGCCTCCGGGgctggtGAGGAGCGGGTAGGGGg
gGTGCCTCGGAGGCCCcgaccaCTCCTCGCCCATCCCCc
    5F+[6]+(1+5F)
human, /basigin) G C ZPU0041 ZPU0042 (N/A) (N/A) Sangamo NHEJ 21930917 R
ZN0178 cCTAGCTCCTGGACTGACCactattGGAGCCTGTAAGTATACTGg
gGATCGAGGACCTGACTGGtgataaCCTCGGACATTCATATGACc
    6F+[6]+(1+6F)
human, DMD G C ZPU0043 ZPU0044 (N/A) (N/A) Sangamo NHEJ 21954090 R
ZN0179 cCGCGTCCGCGCCatggcCATCTACAAGCAg
gGCGCAGGCGCGGtaccgGTAGATGTTCGTc
    4F+[5]+4F
human, TP53 G C ZP10845 ZP10846 TGEK Sharkey-AS+R MA(2F) NHEJ 21983922 R
ZN0180 cCCCAACCAGGTAGTagaaggctGTTGTTCAGATATGGCTGTTa
gGGGTTGGTCCATCAtcttccgaCAACAAGTCTATACCGACAAt
    (2+4F)+[8]+(2+6F)
human, C1GALT1C1 G C ZPU0045 ZPU0046 (N/A) (N/A) Sangamo NHEJ 21983924 R
ZN0181 gTGCTGACCATCGacaagaAAGGGActGAAGCTGCTg
cACGACTGGTAGCtgttctTTCCCTgaCTTCGACGAc
    4F+[6]+(2F+2+3F)
human, A1AT G C ZP10847 ZP10848 LRGS WT Sangamo NHEJ; HR 21993621 R
ZN0184 aGACCCACTGagttggGCAAAGGTGGGGc
tCTGGGTGACtcaaccCGTTTCCACCCCg
    3F+[6]+4F
human, F8, intron G C ZPU0047 ZPU0048 (N/A) (N/A) MA NHEJ-Indel/Inversion 22183967 R
ZN0187 tCAAGTCCAGTTCTACgggctcTCGGAGAATGACGAGTGGa
aGTTCAGGTCAAGATGcccgagAGCCTCTTACTGCTCACCt
    5F+[6]+6F
human, TRBC2/TRBC1 G C ZP10856 ZP10857 LRGS WT Sangamo NHEJ 22466705 R
ZN0188 gTGCTGTGGCCTGgAGCAACaaatctGACTTTGCATGTGCAa
cACGACACCGGACcTCGTTGtttagaCTGAAACGTACACGTt
    (2F+1+4F)+[6]+5F
human, TRAC G C ZP10858 ZP10859 LRGS WT Sangamo NHEJ 22466705 R
ZN0201 gGTCGGCCCTACTCAGGACcgtggtCAGGTGAGGCCAGGAGATg
cCAGCCGGGATGAGTCCTGgcaccaGTCCACTCCGGTCCTCTAc
    6F+[6]+6F
human, GALNT2, exon-intron G C ZPU0051 ZPU0052 (N/A) (N/A) Sangamo NHEJ 22566642 R
ZNU016 (N/A)
    
human, TAP2 G C (N/A) (N/A) (N/A) (N/A) Sangamo HR 22875043 R
ZN0209 tTTCTCCCCAGGTGGCTGCgagccTACGAGAGTGCTGTCt
aAAGAGGGGTCCACCGACGctcggATGCTCTCACGACAGa
    6F+[5]+5F
human, PORCN, intron-exon G C ZPU0063 ZPU0064 (N/A) (N/A) Sangamo NHEJ 22888000 R
ZN0213 gCGACGGGGCCCACGCgctgagCCGGCGGTCGGTGCTg
cGCTGCCCCGGGTGCGcgactcGGCCGCCAGCCACGAc
    5F+[6]+5F
human, METTL21D G C ZPU0071 ZPU0072 (N/A) (N/A) Sangamo NHEJ 22948820 R
ZN0219 cACCTCCTCCccggccGGCGGCGGAc
gTGGAGGAGGggccggCCGCCGCCTg
    3F+[6]+3F
human, VEGFA G C ZP10917 - ZP10920 ZP10920 - ZP10923 LRGS D+R OPEN HR 23028823 R
ZN0220 aGTCACCTTCcctgagTGGGCTGGTa
tCAGTGGAAGggactcACCCGACCAt
    3F+[6]+3F
human, GRN, intron G C ZP10924 - ZP10925 ZP10928 - ZP10934 LRGS D+R OPEN HR 23028823 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
GSearch EENdb with this gene symbol
RSearch EENdb with this reference

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School of Life Sciences, Peking University, Beijing, China
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