EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting sequences from human (Homo sapiens): 96 ZFN, 121 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0001 cGGAGCCATCTGGCCGGGTtggctggttataACCGCGCAGATTCTGTTCa
gCCTCGGTAGACCGGCCCAaccgaccaatatTGGCGCGTCTAAGACAAGt
    18+[12]+18
human, NTF3, 5'-Up G C (NK) +63, +28 WT (N/A) NHEJ, NHEJ-ODC 21179091 R
TN0002 tCTTCATTACACCTg...cATACAGTCAGTatcaattctggaagaa
aGAAGTAATGTGGAc...gTATGTCAGTCAtagttaagaccttctt
    13+[16]+11
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0002B tctTCATTACACCTGCAGCTc...tACAGTCAGTATCAattctggaagaa
agaAGTAATGTGGACGTCGAg...aTGTCAGTCATAGTtaagaccttctt
    17+[12]+13
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0002C tcttcattACACCTGCAGCTCTc...cAGTATCAATTCTGGAAGaa
agaagtaaTGTGGACGTCGAGAg...gTCATAGTTAAGACCTTCtt
    14+[16]+17
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0002D tcttcattACACCTGCAGCTCTCATt...tATCAATTCTGGAAGAa
agaagtaaTGTGGACGTCGAGAGTAa...aTAGTTAAGACCTTCTt
    17+[16]+15
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0003 tGCTGGTCATCCTCATcctgataaactgcAAAAGGCTGAAGAGCatgactgaca
aCGACCAGTAGGAGTAggactatttgacgTTTTCCGACTTCTCGtactgactgt
    15+[13]+15
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TN0003B tgctGGTCATCCTCATCCTgataaactgcaaAAGGCTGAAGAGCatgactgaca
acgaCCAGTAGGAGTAGGActatttgacgttTTCCGACTTCTCGtactgactgt
    15+[12]+13
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TN0003C tgctggtCATCCTCATCCTGATaaactgcaaaAGGCTGAAGAGCATGactgaca
acgaccaGTAGGAGTAGGACTAtttgacgtttTCCGACTTCTCGTACtgactgt
    15+[10]+15
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TN0003D tgctggtcatCCTCATCCTGATAAACTgcaaaaggctgaAGAGCATGACTGACa
acgaccagtaGGAGTAGGACTATTTGAcgttttccgactTCTCGTACTGACTGt
    17+[12]+14
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TN0009 tGCATACCTAATCATTATgctgaggatttggaaAGGGTGTTTATTCCTCATGGa
aCGTATGGATTAGTAATAcgactcctaaaccttTCCCACAAATAAGGAGTACCt
    17+[15]+20
human, HPRT1 G C (std.) +231 WT GG(V&B) 21493687 R
TN0010 tCCATTCCTATGACTGTAGATtttatcagactgaagAGCTATTGTGTGAGTATa
aGGTAAGGATACTGACATCTAaaatagtctgacttcTCGATAACACACTCATAt
    20+[15]+17
human, HPRT1 G C (std.) +231 WT GG(V&B) NHEJ, HR 21493687 R
TN0011 tGGATTATGCCTGGCACCATTAAAGAAAATa...tATAGATACAGAAGCGTCATCAAAGCATGCCa
aCCTAATACGGACCGTGGTAATTTCTTTTAt...aTATCTATGTCTTCGCAGTAGTTTCGTACGGt
    29+[28]+30
human, CFTR G C (others) +231 WT GG(V&B) 21493687 R
TN0012 tGGCACCATTAAAGAAAATATCATc...tAGATACAGAAGCGTCATCAAAGCATGCCa
aCCGTGGTAATTTCTTTTATAGTAg...aTCTATGTCTTCGCAGTAGTTTCGTACGGt
    23+[25]+28
human, CFTR G C (std.) +231 WT GG(V&B) (Weak) 21493687 R Low
TN0025 tCCCCTCCACCCCACAGTggggccactagggacAGGATTGGTGACAGAAAa
aGGGGAGGTGGGGTGTCAccccggtgatccctgTCCTAACCACTGTCTTTt
    17+[15]+17
human, PPP1R12C, intron G C (std.) +63 WT; ELD+KKR (N/A) NHEJ, HR 21738127 R
TN0025-T002 tTCCACCCAGCCCTCAGTgctctgctgaggcAGGATGGGTGGCTGGAAa
aAGGTGGGTCGGGAGTCAcgagacgactccgTCCTACCCACCGACCTTt
    17+[13]+17
human, [intergenic] C +63 ELD+KKR (N/A) NHEJ 21738127 R
TN0025-T003 cCCCCTCCACCCCAAAAAt...aAATATGAGTTATAGAAAa
gGGGGAGGTGGGGTTTTTa...tTTATACTCAATATCTTTt
    17+[24]+17
human, [intergenic] C +63 ELD+KKR (N/A) NHEJ 21738127 R
TN0026 tCAGACGCTGGCACTccgcacccccagctcccagagcACGGCTGCAAGGGCCa
aGTCTGCGACCGTGAggcgtgggggtcgagggtctcgTGCCGACGTTCCCGGt
    14+[22]+15
human, PITX3 G C (std.) +63 WT (N/A) NHEJ, HR 21738127 R
TN0027 tACGCCGTGGAAAGGCCcgtatgagcggccccgcccGTAGATCATCCCCGa
aTGCGGCACCTTTCCGGgcatactcgccggggcgggCATCTAGTAGGGGCt
    16+[19]+14
human, PITX3, 3'-UTR G C (std.) +63 WT (N/A) NHEJ, HR 21738127 R
TN0028 tCACCTGCAGCTGCCCAGacctggcacccaggagAGGAGCAGGCAGGGTCa
aGTGGACGTCGACGGGTCtggaccgtgggtcctcTCCTCGTCCGTCCCAGt
    17+[16]+16
human, POU5F1, intron G C (std.) +63 WT (N/A) NHEJ, HR 21738127 R
TN0029 tCTGGGCTCTCCCATgcattcaaactgaggtgcctgcCCTTCTAGGAATGGGGGa
aGACCCGAGAGGGTAcgtaagtttgactccacggacgGGAAGATCCTTACCCCCt
    14+[22]+17
human, POU5F1, 3'-UTR G C (std.) +63 WT (N/A) NHEJ, HR 21738127 R
TN0030 tACACGTTTCGTGTTCGGAgccgctttaacccacTCTGTGGAAGTGCTCAGCa
aTGTGCAAAGCACAAGCCTcggcgaaattgggtgAGACACCTTCACGAGTCGt
    18+[15]+18
human, IL2RG G C (std.) +47, +17 KV+EA (N/A) NHEJ 21813459 R
TN0031 tGTGGGCAACATGCTGGTCatcctcatcctgataAACTGCAAAAGGCTGAAGa
aCACCCGTTGTACGACCAGtaggagtaggactatTTGACGTTTTCCGACTTCt
    18+[15]+18
human, CCR5 G C (std.) +47, +17 KV+EA (N/A) NHEJ 21813459 R
TN0031-T002 tGTGGGCAACATGCTGGTCgtcctcatcttaataAACTGCAAAAAGCTGAAGt
aCACCCGTTGTACGACCAGcaggagtagaattatTTGACGTTTTTCGACTTCa
    18+[15]+18
human, CCR2 G C +47, +17 KV+EA (N/A) NHEJ 21813459 R Low
TN0037 tGCATCAACCCCATCATCtatgcctttgtcGGGGAGAAGTTCAGAAACTa
aCGTAGTTGGGGTAGTAGatacggaaacagCCCCTCTTCAAGTCTTTGAt
    17+[12]+19
human, CCR5 G C (N/A) (N/A) (N/A) NHEJ 21983922 R
TN0040 tAGCAACCTCAAACAGACACCATg...cTGTGGAGAAGTCTGCCGTTa
aTCGTTGGAGTTTGTCTGTGGTAc...gACACCTCTTCAGACGGCAAt
    22+[15]+19
human, HBB G C (N*; others) +31, +63, +31, +31 WT (N/A) NHEJ 22301904 R
TN0042 tACCTATTATTACTTTATggggcagcagcctggaaAAGTACTTGGGGACCAa
aTGGATAATAATGAAATAccccgtcgtcggaccttTTCATGAACCCCTGGTt
    17+[17]+16
human, ABL1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0043 tGTGTCTTGGGATGAGTGggtcagtgttctggtgCTCACAGGATGGCTGGCa
aCACAGAACCCTACTCACccagtcacaagaccacGAGTGTCCTACCGACCGt
    17+[16]+17
human, AKT2 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0044 tCCTGTGGCTCCTGCCGCtgctgctttccacggcAGCTGTGGGCTCCGGGa
aGGACACCGAGGACGGCGacgacgaaaggtgccgTCGACACCCGAGGCCCt
    17+[16]+16
human, ALK G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0045 tATGTACGCCTCCCTGGGctcgggtccggtcgccCCTTTGCCCGCTTCTGTa
aTACATGCGGAGGGACCCgagcccaggccagcggGGAAACGGGCGAAGACAt
    17+[16]+17
human, APC, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0046 tGAATTGGGATGCTGTTTttaggtattctattcaaaTTTATTTTACTGTCTTTa
aCTTAACCCTACGACAAAaatccataagataagtttAAATAAAATGACAGAAAt
    17+[18]+17
human, ATM G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0047 tCCCTCACCATGAGTAGCgctatgttggtgacttGCCTCCCGGACCCCAGCa
aGGGAGTGGTACTCATCGcgatacaaccactgaaCGGAGGGCCTGGGGTCGt
    17+[16]+17
human, AXIN2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0048 tGTGCGATCTCCAAGCACtgaggggcagaaactcCCGGATCGGGCGCTGCCa
aCACGCTAGAGGTTCGTGactccccgtctttgagGGCCTAGCCCGCGACGGt
    17+[16]+17
human, BAX G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0049 tTTTCAAGTGAAGACAAAatggcctcgccggctgACAGCTGTATCCAGTTCa
aAAAGTTCACTTCTGTTTtaccggagcggccgacTGTCGACATAGGTCAAGt
    17+[16]+17
human, BCL6, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0050 tACAATTGAACAATGCCTcagctatacatttacatCAGATTATTGGGAGCCTa
aTGTTAACTTGTTACGGAgtcgatatgtaaatgtaGTCTAATAACCCTCGGAt
    17+[17]+17
human, BMPR1A, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0051 tCCGAAGCTGACAGATGGgtattctttgacggggGGTAGGGGCGGAACCTGa
aGGCTTCGACTGTCTACCcataagaaactgccccCCATCCCCGCCTTGGACt
    17+[16]+17
human, BRCA1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0052 tTAGACTTAGGTAAGTAAtgcaatatggtagactGGGGAGAACTACAAACTa
aATCTGAATCCATTCATTacgttataccatctgaCCCCTCTTGATGTTTGAt
    17+[16]+17
human, BRCA2 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0053 tCTGCAATAAAAAATGGCctccaacaaaactacaTTGGTAAGTTAATGAAAa
aGACGTTATTTTTTACCGgaggttgttttgatgtAACCATTCAATTACTTTt
    17+[16]+17
human, CBX3, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0054 tGGAGCTTTCAGCGGTGGgggagcgggtgttcgcgGCCGAAGCCCTCCTGAa
aCCTCGAAAGTCGCCACCccctcgcccacaagcgcCGGCTTCGGGAGGACTt
    17+[17]+16
human, CBX8, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0055 tGGAACACCAGCTCCTGTgctgcgaagtggaaaccatCCGCCGCGCGTACCCCGa
aCCTTGTGGTCGAGGACAcgacgcttcacctttggtaGGCGGCGCGCATGGGGCt
    17+[19]+17
human, CCND1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0056 tGCTTAGCGTCCTGCGACagtacaacatccagaaGAAGGAGATTGTGGTGAa
aCGAATCGCAGGACGCTGtcatgttgtaggtcttCTTCCTCTAACACCACTt
    17+[16]+17
human, CDC73 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0057 tGCTGCAGGTACCCCGGAtcccctgacttgcgagGGACGCATTCGGGCCGCa
aCGACGTCCATGGGGCCTaggggactgaacgctcCCTGCGTAAGCCCGGCGt
    17+[16]+17
human, CDH1 G C (std.) +63 WT FLASH 22484455 R No
TN0058 tCCCTTGATCTGAGAAtggctacctctcgataTGAGCCAGTGGCTGAAa
aGGGAACTAGACTCTTaccgatggagagctatACTCGGTCACCGACTTt
    15+[16]+16
human, CDK4, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0059 tGGCGTCGGGCCTGGGCtccccgtccccctgctcGGCGGGCAGTGAGGAGGa
aCCGCAGCCCGGACCCGaggggcagggggacgagCCGCCCGTCACTCCTCCt
    16+[17]+17
human, CHD4, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0060 tGTGTTGGAAGAAGATGGcagatccaggaatgatGAGTCTTTTTGGCGAGGa
aCACAACCTTCTTCTACCgtctaggtccttactaCTCAGAAAAACCGCTCCt
    17+[16]+17
human, CHD7, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0061 tCCAGCGTGGACAATGGctactcaaggtttgtgTCATTAAATCTTTAGTTa
aGGTCGCACCTGTTACCgatgagttccaaacacAGTAATTTAGAAATCAAt
    16+[16]+17
human, CTNNB1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0062 tAATATCACAATGAGTTCaggcttatggagccaagaAAAAGTCACTTCACCCTa
aTTATAGTGTTACTCAAGtccgaatacctcggttctTTTTCAGTGAAGTGGGAt
    17+[18]+17
human, CYLD, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0063 tCACACGGAGGACGCGatggctcccaagaaacGCCCAGAAACCCAGAAGa
aGTGTGCCTCCTGCGCtaccgagggttctttgCGGGTCTTTGGGTCTTCt
    15+[16]+17
human, DDB2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0064 tCCGGCCGGCGCCATGAagtgagaagggggctgGGGGTCGCGCTCGCTa
aGGCCGGCCGCGGTACTtcactcttcccccgacCCCCAGCGCGAGCGAt
    16+[16]+15
human, ERCC2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0065 tCCGGGATCGCCATGGGAactcaatagaaaatcctcaTCTTCTCACTTTGTTTCa
aGGCCCTAGCGGTACCCTtgagttatcttttaggagtAGAAGAGTGAAACAAAGt
    17+[19]+17
human, ERCC5 G C (std.) +63 WT FLASH 22484455 R No
TN0066 tGGCGTCCACGGGTGAGTatggtggaactgcggtcGCGCCGGCGGTAGCCGGa
aCCGCAGGTGCCCACTCAtaccaccttgacgccagCGCGGCCGCCATCGGCCt
    17+[17]+17
human, EWSR1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0067 tGACCCAGGCAGGACACAtgcaggccaaaaaacgcTATTTCATCCTGCTCTCa
aCTGGGTCCGTCCTGTGTacgtccggttttttgcgATAAAGTAGGACGAGAGt
    17+[17]+17
human, EXT1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0068 tTCCTCCCAGGGGGATGTcctgcgcctcagggtcCGGTGGTGGCCTGCGGCa
aAGGAGGGTCCCCCTACAggacgcggagtcccagGCCACCACCGGACGCCGt
    17+[16]+17
human, EXT2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0069 tGCTTTTAGAATAATCATgggccagactgggaagAAATCTGAGAAGGGACCa
aCGAAAATCTTATTAGTAcccggtctgacccttcTTTAGACTCTTCCCTGGt
    17+[16]+17
human, EZH2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0070 tAGGCGCCAAGGCCATGTccgactcgtgggtcccGAACTCCGCCTCGGGCCa
aTCCGCGGTTCCGGTACAggctgagcacccagggCTTGAGGCGGAGCCCGGt
    17+[16]+17
human, FANCA, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0071 tGAAGGGACATCACCTTTtcgctttttccaagatgGCTCAAGATTCAGTAGa
aCTTCCCTGTAGTGGAAAagcgaaaaaggttctacCGAGTTCTAAGTCATCt
    17+[17]+16
human, FANCC, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0072 tGCCCCGGCATGGCGACAccggacgcggggctcccTGGGGCTGAGGGCGTGGa
aCGGGGCCGTACCGCTGTggcctgcgccccgagggACCCCGACTCCCGCACCt
    17+[17]+17
human, FANCE, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0073 tTCGCGCACCTCATGGaatcccttctgcagcaCCTGGATCGCTTTTCCGa
aAGCGCGTGGAGTACCttagggaagacgtcgtGGACCTAGCGAAAAGGCt
    15+[16]+17
human, FANCF, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0074 tCGGCCACCATGTCCCgccagaccacctctgtGGGCTCCAGCTGCCTGGa
aGCCGGTGGTACAGGGcggtctggtggagacaCCCGAGGTCGACGGACCt
    15+[16]+17
human, FANCG, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0075 tCCCCAGAACAGCACTATgggcttctcttccgagctGTGCAGCCCCCAGGGCCa
aGGGGTCTTGTCGTGATAcccgaagagaaggctcgaCACGTCGGGGGTCCCGGt
    17+[18]+17
human, FES, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0076 tCTGCTCCCCACCGAGGAcctctgcatgcaggcaTGAATCCCAGGAGCCTa
aGACGAGGGGTGGCTCCTggagacgtacgtccgtACTTAGGGTCCTCGGAt
    17+[16]+16
human, FGFR1 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0077 tGTACCGAGCACTTCGGCtcctcgcgcgctcgcgtCCCCTCGTGCGGGCTCCa
aCATGGCTCGTGAAGCCGaggagcgcgcgagcgcaGGGGAGCACGCCCGAGGt
    17+[17]+17
human, FH, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0078 tCTCCAAGGCACCATGAAtgccatcgtggctctctgCCACTTCTGCGAGCTCCa
aGAGGTTCCGTGGTACTTacggtagcaccgagagacGGTGAAGACGCTCGAGGt
    17+[18]+17
human, FLCN, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0079 tCCGGAGGCCATGCCGGCg...aGCTGCCGCTGCTCGGTa
aGGCCTCCGGTACGGCCGc...tCGACGGCGACGAGCCAt
    17+[21]+16
human, FLT3, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0080 tGCAGCGGGGCGCCGCGCtgtgcctgcgactgtggctCTGCCTGGGACTCCTGGa
aCGTCGCCCCGCGGCGCGacacggacgctgacaccgaGACGGACCCTGAGGACCt
    17+[19]+17
human, FLT4, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0081 tCACCATGGCCGAGGCGcctcaggtggtggagaTCGACCCGGACTTCGa
aGTGGTACCGGCTCCGCggagtccaccacctctAGCTGGGCCTGAAGCt
    16+[16]+15
human, FOXO1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0082 tCTCCGCTCGAAGTGGAGctggacccggagttcgagCCCCAGAGCCGTCCGCGa
aGAGGCGAGCTTCACCTCgacctgggcctcaagctcGGGGTCTCGGCAGGCGCt
    17+[18]+17
human, FOXO3 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0083 tCCTCTGAGACGCCATGTtcaactcgatgaccccACCACCAATCAGTAGCTa
aGGAGACTCTGCGGTACAagttgagctactggggTGGTGGTTAGTCATCGAt
    17+[16]+17
human, GLI1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0084 tGGCGCAGACGCAGGGCacccggaggaaagtctgTTACTACTACGACGGTGa
aCCGCGTCTGCGTCCCGtgggcctcctttcagacAATGATGATGCTGCCACt
    16+[17]+17
human, HDAC1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0085 tGCGCTCACCTCCCTGCGgcctcctgaggtggtttgGTGGCCCCCTCCTCGCGa
aCGCGAGTGGAGGGACGCcggaggactccaccaaacCACCGGGGGAGGAGCGCt
    17+[18]+17
human, HDAC2 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0086 tCCTCAACTATGACCTCAaccggccaggattccaCCACAACCAGGCAGCGAa
aGGAGTTGATACTGGAGTtggccggtcctaaggtGGTGTTGGTCCGTCGCTt
    17+[16]+17
human, HDAC6, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0087 tGAGCGCACGCGGTGAGGgcgcggggcagccgtcCACTTCAGCCCAGGGACa
aCTCGCGTGCGCCACTCCcgcgccccgtcggcagGTGAAGTCGGGTCCCTGt
    17+[16]+17
human, HMGA2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0088 tCCGTGCTCCTCCACCCCcgctggatcgagcccacCGTCATGTTTCTCTACGa
aGGCACGAGGAGGTGGGGgcgacctagctcgggtgGCAGTACAAAGAGATGCt
    17+[17]+17
human, HOXA13 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0089 tGGGCACGGTGATGGCcaccactggggccctgGGCAACTACTACGTGGa
aCCCGTGCCACTACCGgtggtgaccccgggacCCGTTGATGATGCACCt
    15+[16]+16
human, HOXA9, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0090 tCCAGCAGATCATGTCATgacgacttcgctgctcctGCATCCACGCTGGCCGGa
aGGTCGTCTAGTACAGTActgctgaagcgacgaggaCGTAGGTGCGACCGGCCt
    17+[18]+17
human, HOXC13, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0091 tTGACGAGTGCGGCCAGagcgcagccagcatgtaCCTGCCGGGCTGCGCCTa
aACTGCTCACGCCGGTCtcgcgtcggtcgtacatGGACGGCCCGACGCGGAt
    16+[17]+17
human, HOXD11 G C (std.) +63 WT FLASH 22484455 R No
TN0092 tGCGGGCAGACGGCGGGGgcgccggtggcgccccgGCCTCTTCCTCCTCCTCa
aCGCCCGTCTGCCGCCCCcgcggccaccgcggggcCGGAGAAGGAGGAGGAGt
    17+[17]+17
human, HOXD13 G C (std.) +63 WT FLASH 22484455 R No
TN0093 tCTGAAAAAGACTCTGCAtgggaatggcctgcctTACGATGACAGAAATGGa
aGACTTTTTCTGAGACGTacccttaccggacggaATGCTACTGTCTTTACCt
    17+[16]+17
human, JAK2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0094 tACCGCGATGAGAGGCGCtcgcggcgcctgggattttCTCTGCGTTCTGCTCCTa
aTGGCGCTACTCTCCGCGagcgccgcggaccctaaaaGAGACGCAAGACGAGGAt
    17+[19]+17
human, KIT, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0095 tGAAAATGACTGAATATAaacttgtggtagttggaGCTGGTGGCGTAGGCAa
aCTTTTACTGACTTATATttgaacaccatcaacctCGACCACCGCATCCGTt
    17+[17]+16
human, KRAS, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0096 tAGGGTCCCCGGCGCCAGgccacccggccgtcagCAGCATGCAGGGTAAGGa
aTCCCAGGGGCCGCGGTCcggtgggccggcagtcGTCGTACGTCCCATTCCt
    17+[16]+17
human, MAP2K4 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0097 tCCAAGCGCGAAAACCCCggatggtgaggagcaggTACTGGCCCGGCAGCGa
aGGTTCGCGCTTTTGGGGcctaccactcctcgtccATGACCGGGCCGTCGCt
    17+[17]+16
human, MDM2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0098 tTATTATTACATGGCTTTgccttactgaggcttcATCTTGTCCTCTGGTCCa
aATAATAATGTACCGAAAcggaatgactccgaagTAGAACAGGAGACCAGGt
    17+[16]+17
human, MET G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0099 tCTGGCGCCAAAATGTCGttcgtggcaggggttaTTCGGCGGCTGGACGAGa
aGACCGCGGTTTTACAGCaagcaccgtccccaatAAGCCGCCGACCTGCTCt
    17+[16]+17
human, MLH1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0100 tGAGGAGGTTTCGACATGgcggtgcagccgaaggAGACGCTGCAGTTGGAGa
aCTCCTCCAAAGCTGTACcgccacgtcggcttccTCTGCGACGTCAACCTCt
    17+[16]+17
human, MSH2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0101 tCACTGTCGGCGGCCATGacaccgctcgtctcccgcCTGAGTCGTCTGTGGGTa
aGTGACAGCCGCCGGTACtgtggcgagcagagggcgGACTCAGCAGACACCCAt
    17+[18]+17
human, MUTYH, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0102 tGCTTAGACGCTGGATTTttttcgggtagtggaaAACCAGGTAAGCACCGAa
aCGAATCTGCGACCTAAAaaaagcccatcaccttTTGGTCCATTCGTGGCTt
    17+[16]+17
human, MYC G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0103 tCCCGCAGGGAGCGGACAtggactacgactcgtaCCAGCACTATTTCTACGa
aGGGCGTCCCTCGCCTGTacctgatgctgagcatGGTCGTGATAAAGATGCt
    17+[16]+17
human, MYCL1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0104 tGCCGAGCTGCTCCACgtccaccatgccgggcATGATCTGCAAGAACCCa
aCGGCTCGACGAGGTGcaggtggtacggcccgTACTAGACGTTCTTGGGt
    15+[16]+17
human, MYCN, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0105 tGAGGAGCCGGACCGAtgtggaaactgctgccCGCCGCGGGCCCGGCa
aCTCCTCGGCCTGGCTacacctttgacgacggGCGGCGCCCGGGCCGt
    15+[16]+15
human, NBN, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0106 tCTTTACTGATAATGTCAagttcaggttatcctcCCAACCAAGGAGCATTCa
aGAAATGACTATTACAGTtcaagtccaataggagGGTTGGTTCCTCGTAAGt
    17+[16]+17
human, NCOR1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0107 tGGAGGGCCACTGAGCcccgctacccgccccaCAGCCTTTCCTACCCa
aCCTCCCGGTGACTCGgggcgatgggcggggtGTCGGAAAGGATGGGt
    15+[16]+15
human, NCOR2 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0108 tCGGCGCATGAAGGAGGTactcctcattttcgttCTCTCTCTCTGTGCCCCa
aGCCGCGTACTTCCTCCAtgaggagtaaaagcaaGAGAGAGAGACACGGGGt
    17+[16]+17
human, NTRK1, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0109 tTGCGCTCGGGGCGGCCAtgtcggccggcgaggtCGAGCGCCTAGTGTCGGa
aACGCGAGCCCCGCCGGTacagccggccgctccaGCTCGCGGATCACAGCCt
    17+[16]+17
human, PDGFRA, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0110 tCTGCAGGACACCATGCGgcttccgggtgcgatgCCAGCTCTGGCCCTCAAa
aGACGTCCTGTGGTACGCcgaaggcccacgctacGGTCGAGACCGGGAGTTt
    17+[16]+17
human, PDGFRB, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0111 tGAGTACTCCGCCTCTACcccggctgaagcccgcCCCCGCCGCCACCTATTa
aCTCATGAGGCGGAGATGgggccgacttcgggcgGGGGCGGCGGTGGATAAt
    17+[16]+17
human, PHF8 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0112 tCGGGTGTTGCATCCATGgagcgagctgagagctcgAGGTGAGCGGGGCTCGCa
aGCCCACAACGTAGGTACctcgctcgactctcgagcTCCACTCGCCCCGAGCGt
    17+[18]+17
human, PMS2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0113 tGGAACTGCTTAATAGaaacaggcttgtaattGTGAGTCCGCGCTGCa
aCCTTGACGAATTATCtttgtccgaacattaaCACTCAGGCGCGACGt
    15+[16]+15
human, PTCH1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0114 tCCCAGACATGACAGCCatcatcaaagagatcgTTAGCAGAAACAAAAGGa
aGGGTCTGTACTGTCGGtagtagtttctctagcAATCGTCTTTGTTTTCCt
    16+[16]+17
human, PTEN, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0115 tGGCATGGCCAGCAACAGcagctcctgcccgacacCTGGGGGCGGGCACCTCa
aCCGTACCGGTCGTTGTCgtcgaggacgggctgtgGACCCCCGCCCGTGGAGt
    17+[17]+17
human, RARA, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0116 tGCTGGGTGAGAAGGGCtgtggctgcgttttagaGAAGCGTTGGGTACTGGa
aCGACCCACTCTTCCCGacaccgacgcaaaatctCTTCGCAACCCATGACCt
    16+[17]+17
human, RBBP5 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0117 tGCGGGACGTGCGGGAGCggctgcaggcgtgggaGCGCGCGTTCCGACGGCa
aCGCCCTGCACGCCCTCGccgacgtccgcaccctCGCGCGCAAGGCTGCCGt
    17+[16]+17
human, RECQL4 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0118 tCAGAATACAGTTATGGCcacccaggtaatggggCAGTCTTCTGGAGGAGGa
aGTCTTATGTCAATACCGgtgggtccattaccccGTCAGAAGACCTCCTCCt
    17+[16]+17
human, REST, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0119 tGAGTTCTGCCGGCCGCCggctcccgcaggggccaGGGCGAAGTTGGCGCCGa
aCTCAAGACGGCCGGCGGccgagggcgtccccggtCCCGCTTCAACCGCGGCt
    17+[17]+17
human, RET G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0120 tTCTTTATTTCCAGCAATgtctcaggctgtgcagACAAACGGAACTCAACCa
aAGAAATAAAGGTCGTTAcagagtccgacacgtcTGTTTGCCTTGAGTTGGt
    17+[16]+17
human, RNF2, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0121 tTCAGGAGGAAGCGATGGcttcagacagcatattTGAGTCATTTCCTTCGTa
aAGTCCTCCTTCGCTACCgaagtctgtcgtataaACTCAGTAAAGGAAGCAt
    17+[16]+17
human, RUNX1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0122 tCTCCTTGAGGCGCCGGTtgccggccacaaccctTGGCGGAGCCTGCCTGCa
aGAGGAACTCCGCGGCCAacggccggtgttgggaACCGCCTCGGACGGACGt
    17+[16]+17
human, SDHB G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0123 tGTTGCTGAGGTGACTTCagtgggactgggagttggtGCCTGCGGCCCTCCGGa
aCAACGACTCCACTGAAGtcaccctgaccctcaaccaCGGACGCCGGGAGGCCt
    17+[19]+16
human, SDHC G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0124 tCAGGAACGAGATGGCGGttctctggaggctgagtGCCGTTTGCGGTGCCCTa
aGTCCTTGCTCTACCGCCaagagacctccgactcaCGGCAAACGCCACGGGAt
    17+[17]+17
human, SDHD, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0125 tGCAGAGGACAAAAGCATgtcttcccttcctgggTGCATTGGTTTGGATGCa
aCGTCTCCTGTTTTCGTAcagaagggaaggacccACGTAACCAAACCTACGt
    17+[16]+17
human, SETDB1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0126 tTACGCGGCGGGGCTGTCgccgtacgcggacaagggCAAGTGCGGCCTCCCGGa
aATGCGCCGCCCCGACAGcggcatgcgcctgttcccGTTCACGCCGGAGGGCCt
    17+[18]+17
human, SIRT6 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0127 tTTGGTAAGAACATGTCGtccatcttgccattcacGCCGCCAGTTGTGAAGa
aAACCATTCTTGTACAGCaggtagaacggtaagtgCGGCGGTCAACACTTCt
    17+[17]+16
human, SMAD2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0128 tGGTGACGGCGGCAACATgtctgtggctttcgcggCCCCGAGGCAGCGAGGCa
aCCACTGCCGCCGTTGTAcagacaccgaaagcgccGGGGCTCCGTCGCTCCGt
    17+[17]+17
human, SS18, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0129 tGGCGCCTCAGAAGCAcggcggtgggggagggGGCGGCTCGGGGCCCa
aCCGCGGAGTCTTCGTgccgccaccccctcccCCGCCGAGCCCCGGGt
    15+[16]+15
human, SUZ12, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0130 tCATGTCTCATGCGGCCGaaccagctcgggatggCGTAGAGGCCAGCGCGGa
aGTACAGAGTACGCCGGCttggtcgagccctaccGCATCTCCGGTCGCGCCt
    17+[16]+17
human, TFE3, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0131 tCGGGGGCTGCTCAGGGGcctgtggccgctgcacaTCGTCCTGTGGACGCGTa
aGCCCCCGACGAGTCCCCggacaccggcgacgtgtAGCAGGACACCTGCGCAt
    17+[17]+17
human, TGFBR2 G C (std.) +63 WT FLASH 22484455 R No
TN0132 tTCCGCCCGCCCAGGATGgaggcgcccgccagcgcGCAGACCCCGCACCCGCa
aAGGCGGGCGGGTCCTACctccgcgggcggtcgcgCGTCTGGGGCGTGGGCGt
    17+[17]+17
human, TLX3, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0133 tTGCCGTCCCAAGCAATGgatgatttgatgctgtcCCCGGACGATATTGAACa
aACGGCAGGGTTCGTTACctactaaactacgacagGGGCCTGCTATAACTTGt
    17+[17]+17
human, TP53 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0134 tCCTGGTCCACCATGGCcaaaccaacaagcaaagATTCAGGCTTGAAGGAGa
aGGACCAGGTGGTACCGgtttggttgttcgtttcTAAGTCCGAACTTCCTCt
    16+[17]+17
human, TSC2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0135 tCTGGATCGCGGAGGGAAtgccccggagggcggaGAACTGGGACGAGGCCGa
aGACCTAGCGCCTCCCTTacggggcctcccgcctCTTGACCCTGCTCCGGCt
    17+[16]+17
human, VHL, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0136 tGGGCCAGAGATGGCGGCggccgacggggctttgCCGGAGGCGGCGGCTTTa
aCCCGGTCTCTACCGCCGccggctgccccgaaacGGCCTCCGCCGCCGAAAt
    17+[16]+17
human, XPA, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0137 tGCCCAGACAAGCAACATggctcggaaacgcgcggccGGCGGGGAGCCGCGGGGa
aCGGGTCTGTTCGTTGTAccgagcctttgcgcgccggCCGCCCCTCGGCGCCCCt
    17+[19]+17
human, XPC, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0003 tCGGAGCCGCTTTaacccACTCTGTGGAAGt
aGCCTCGGCGAAAttgggTGAGACACCTTCa
    4F+[5]+4F
human, IL2RG G C ZP10005 ZP10006 (N/A) WT Sangamo HR 15806097 R
ZN0006 tGTCCCCTTCtgggctcactatGCTGCCGCCc
aCAGGGGAAGacccgagtgataCGACGGCGGg
    3F+[12]+3F
human, CCR5 G C ZP10013 ZP10014 TG(G4S)3 WT MA 16084494 R
ZN0007 gGCCCGCCCCctagcgGCCGGGGAGg
cCGGGCGGGGgatcgcCGGCCCCTCc
    3F+[6]+3F
human, DMPK, 3'-UTR G C ZP10012 ZP10011 TG WT MA 16084494 R
ZN0009 cGGCGCCCACcatcgaGTCGCAGCCc
gCCGCGGGTGgtagctCAGCGTCGGg
    3F+[6]+3F
human, CD8A G C ZP10020 ZP10021 (3aa) WT MA HR 16169774 R
ZN0018 cCTACTCaTTCAACcaataGCCCGGGCCGTAc
gGATGAGtAAGTTGgttatCGGGCCCGGCATg
    (2F+1+2F)+[5]+4F
human, (mtDNA) G C ZP10051, ZP10052; ZP10053 - ZP10057 ZP10061, ZP10062; ZP10063 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018-T002 cCTACTCaTTCAACcaataGCCCTGGCCGTAc
gGATGAGtAAGTTGgttatCGGGACCGGCATg
    (2F+1+2F)+[5]+4F
human, (mtDNA) G C ZP10053 - ZP10057 ZP10061-T002 - ZP10063-T002 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018B cAGCCTACTCaTTCAACcaataGCCCGGGCCGTAc
gTCGGATGAGtAAGTTGgttatCGGGCCCGGCATg
    (2F+1+3F)+[5]+4F
human, (mtDNA) G C ZP10058 ZP10061 - ZP10063 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018B-T002 cAGCCTACTCaTTCAACcaataGCCCTGGCCGTAc
gTCGGATGAGtAAGTTGgttatCGGGACCGGCATg
    (2F+1+3F)+[5]+4F
human, (mtDNA) G C ZP10058 ZP10061-T002 - ZP10063-T002 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018C
(Z-S1)
GCCCGGGCCGTAc
CGGGCCCGGCATg
    4F
human, (mtDNA) [mutated] G C ZP10063 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZNU017
(Z-S1)
(N/A)
    
human, (mtDNA) G C S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0019 gGTCATCCTCATCctgatAAACTGCAAAAGg
cCAGTAGGAGTAGgactaTTTGACGTTTTCc
    4F+[5]+4F
human, CCR5 G C ZP10064 ZP10065 LRGS WT, EL+KK Sangamo NHEJ 18587387 R
ZN0019-T002 gGTCGTCCTCATCttaatAAACTGCAAAAAg
cCAGCAGGAGTAGaattaTTTGACGTTTTTc
    4F+[5]+4F
human, CCR2 G C ZP10064-T002 ZP10065-T002 LRGS EL+KK Sangamo NHEJ 18587387 R
ZN0019-T003 aGTCATCCTCATCtcacgGATGAGGATGCCa
tCAGTAGGAGTAGagtgcCTACTCCTACGGt
    4F+[5]+4F
human, ABLIM2, intron G C ZP10064 ZP10064-T003 LRGS EL+KK Sangamo NHEJ 18587387 R Low
ZN0022 tCACTTCTAAtggtgaTTATGGGAGa
aGTGAAGATTaccactAATACCCTCt
    3F+[6]+3F
human, CFTR, intron G C ZP10153 ZP10165 LRGS WT OPEN NHEJ 18657511 R
ZN0023 cAGCGTCTTCgagagtGAGGACGTGt
gTCGCAGAAGctctcaCTCCTGCACa
    3F+[6]+3F
human, VEGFA, 5'-up G C ZP10176, ZP10181 ZP10186, ZP10190 LRGS WT, EL+KK OPEN NHEJ; HR 18657511 R
ZN0024 gAGCAGCGTCttcgaGAGTGAGGAc
cTCGTCGCAGaagctCTCACTCCTg
    3F+[5]+3F
human, VEGFA, 5'-up G C ZP10213, ZP10216 ZP10224, ZP10227 LRGS WT, EL+KK OPEN NHEJ; HR 18657511 R
ZN0025 cCCCAGCCGCcccactGAGTTTGCCt
gGGGTCGGCGgggtgaCTCAAACGGa
    3F+[6]+3F
human, HOXB13 G C ZP10280, ZP10282 ZP10284, ZP10285 LRGS WT OPEN NHEJ 18657511 R
ZN0026 aTGCCACCCAtgcccTGGGGTGCCc
tACGGTGGGTacgggACCCCACGGg
    3F+[5]+3F
human, HOXB13 G C ZP10297, ZP10299 ZP10302, ZP10306 LRGS WT OPEN NHEJ 18657511 R
ZN0027 gAGACTCCCAcggccGGGGAAGAGt
cTCTGAGGGTgccggCCCCTTCTCa
    3F+[5]+3F
human, HOXB13 G C ZP10314, ZP10319 ZP10325, ZP10326 LRGS WT OPEN NHEJ 18657511 R
ZN0043 aAGTCCAATCTATgacatcAATTATTATACAt
tTCAGGTTAGATActgtagTTAATAATATGTa
    4F+[6]+4F
human, CCR5 G C ZP10438 ZP10439 TGEK WT MA NHEJ 19470664 R
ZN0044 tCTGGGCTCACTAtgctgcCGCCCAGTGGGAc
aGACCCGAGTGATacgacgGCGGGTCACCCTg
    4F+[6]+4F
human, CCR5 G C ZP10440 ZP10441 TGEK WT MA NHEJ 19470664 R
ZN0045 cTTCTTCATCATCctcctGACAATCGAt
gAAGAAGTAGTAGgaggaCTGTTAGCTa
    4F+[5]+3F
human, CCR5/CCR2 G C ZP10442 ZP10443 TGEK WT MA NHEJ 19470664 R
ZN0046 cTGTGTTTGCTTTaaaagcCAGGACGGTCACc
gACACAAACGAAAttttcgGTCCTGCCAGTGg
    4F+[6]+4F
human, CCR5/CCR2 G C ZP10444 ZP10445 TGEK WT MA NHEJ 19470664 R
ZN0047 cAGGACGGTCacctttGGGGTGGTGa
gTCCTGCCAGtggaaaCCCCACCACt
    3F+[6]+3F
human, CCR5 G C ZP10446 ZP10447 TGEK WT MA NHEJ 19470664 R
ZN0048 aCGGTCACCTttggggTGGTGACAAGTGt
tGCCAGTGGAaaccccACCACTGTTCACa
    3F+[6]+4F
human, CCR5/CCR2 G C ZP10448 ZP10449 TGEK WT MA NHEJ 19470664 R
ZN0049 aCCAAGCTATgcaggtGACAGAGACt
tGGTTCGATAcgtccaCTGTCTCTGa
    3F+[6]+3F
human, CCR5 G C ZP10450 ZP10451 TGEK WT MA NHEJ 19470664 R
ZN0050 cCCCATCATCTATgcctttGTCGGGGAGAAGt
gGGGTAGTAGATAcggaaaCAGCCCCTCTTCa
    4F+[6]+4F
human, CCR5 G C ZP10452 ZP10453 TGEK WT, D+R, EL+KK MA NHEJ 19470664 R
ZN0050-T002 cCCCATCATCTATgccttCGTTGGGGAGAAGt
gGGGTAGTAGATAcggaaGCAACCCCTCTTCa
    4F+[5]+4F
human, CCR2 G C ZP10452 ZP10453-T002 TGEK WT, D+R, EL+KK MA NHEJ 19470664 R Low
ZN0051 aGGCTACATCtttgcTTTGTTGGCa
tCCGATGTAGaaacgAAACAACCGt
    3F+[5]+3F
human, PIGA G C ZP10454; ZP10455 ZP10457; ZP10456 LRGS WT OPEN NHEJ; HR 19540188 R
ZN0055 tACTGTCCCCTTCtgggctcactatGCTGCCGCCCAGt
aTGACAGGGGAAGacccgagtgataCGACGGCGGGTCa
    4F+[12]+4F
human, CCR5 G C ZP10465 ZP10464 QNKKGLD(G4S)3 WT, D+R, EL+KK MA NHEJ 19635463 R
ZN0056 aGCTCTCATTTTCcatacaGTCAGTATCAATt
tCGAGAGTAAAAGgtatgtCAGTCATAGTTAa
    4F+[6]+4F
human, CCR5 G C ZP10467 ZP10466 QNKK WT, D+R, EL+KK MA NHEJ 19635463 R
ZN0057 cACCCCACAGTGGggccacTAGGGACAGGATt
gTGGGGTGTCACCccggtgATCCCTGTCCTAa
    4F+[6]+4F
human, PPP1R12C, intron G C ZP10468 ZP10469 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0058 cCAGACCtGGCACCCAGgagagGAGCAGGCAGGGTCAGCTg
gGTCTGGaCCGTGGGTCctctcCTCGTCCGTCCCAGTCGAc
    (3F+1+2F)+[5]+6F
human, POU5F1, intron G C ZP10470 ZP10471 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0059 cTGCAGCTGCCCAGACCTGgcaccCAGGAGaGGAGCAGGCa
gACGTCGACGGGTCTGGACcgtggGTCCTCtCCTCGTCCGt
    6F+[5]+(2F+1+3F)
human, POU5F1, intron G C ZP10472 ZP10473 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0060 aGTAGTCCTTTGTTACatgcatGAGTCAGTGAACAGGg
tCATCAGGAAACAATGtacgtaCTCAGTCACTTGTCCc
    5F+[6]+5F
human, POU5F1, intron G C ZP10474 ZP10475 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0061 cCCCAGCTCCCAGAGCacggctGCAAGGGCCaGGAGCAc
gGGGTCGAGGGTCTCGtgccgaCGTTCCCGGtCCTCGTg
    5F+[6]+(3F+1+2F)
human, PITX3 G C ZP10477 ZP10476 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0061-T002 tAGCTCACaGTCCCTTGT AAAAGGGCCCaGGGAGCAg
aTCGAGTGtCAGGGAACA TTTTCCCGGGtCCCTCGTc
    5F+5F
human, PKP1, intron G C ZP10477-T002 ZP10477-T003 LRGS EL+KK Sangamo NHEJ 19680244 R Low
ZN0065 aGGCTCCTCCTCCaacattGGGGATTATGATt
tCCGAGGAGGAGGttgtaaCCCCTAATACTAa
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10484 ZP10485 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0066 cTCATCCATTTGCactcctGGGTATGGAGACc
gAGTAGGTAAACGtgaggaCCCATACCTCTGg
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10486 ZP10487 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0067 cATACCCACCTCAcgtgtgGTCAATGGAGTGt
gTATGGGTGGAGTgcacacCAGTTACCTCACa
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10488 ZP10489 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0068 gGTCCCATCACCAgcgatgGATATAGTCTGGa
cCAGGGTAGTGGTcgctacCTATATCAGACCt
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10490 ZP10491 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0069 gGTCTGCAGCCACagctggGTGGATGCAGCTg
cCAGACGTCGGTGtcgaccCACCTACGTCGAc
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10492 ZP10493 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0070 tGACCTCACCACCtctcagTGGGAAGAGGAGg
aCTGGAGTGGTGGagagtcACCCTTCTCCTCc
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10494 ZP10495 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0071 tTTCTATGGCAGCctcaggGGAGAATGGGACt
aAAGATACCGTCGgagtccCCTCTTACCCTGa
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10496 ZP10497 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0138B gGGAGTGgTGCATGGTGtggcaaCAGGTAaGCTCCAtTGTGCTt
cCCTCACcACGTACCACaccgttGTCCATtCGAGGTaACACGAa
    (3F+1+2F)+[6]+(2F+1+2F+1+2F)
human, SNCA G C ZP10755, ZP10756 ZP10759 WT, ELD+KKR Sangamo NHEJ; HR 20120421 R
ZNU001 (N/A)
    
human, KDR G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU002 (N/A)
    
human, TP73 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU003 (N/A)
    
human, MAP3K14 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU004 (N/A)
    
human, EP300 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU005 (N/A)
    
human, BTK G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU006 (N/A)
    
human, CARM1 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU007 (N/A)
    
human, GNAI2 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU008 (N/A)
    
human, TSC2 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU009 (N/A)
    
human, RIPK1 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU010 (N/A)
    
human, NR3C1 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZN0084 aGGCCGCTGCccagcaGCAGGAGTCa
tCCGGCGACGggtcgtCGTCCTCAGt
    3F+[6]+3F
human, RHO G C ZP10543 ZP10542 (G3S)4 WT MA NHEJ; HR 20671268 R
ZN0085 tGCTGCTGCTgctgctgctGCTGCTGCTg
aCGACGACGAcgacgacgaCGACGACGAc
    3F+[3n]+3F
human, (microsatellite) G C ZP10545 ZP10544 (N/A) WT MA HR 20676085 R
ZN0112 gATGGGCAGCGCCcgagtGGCGGAGCTGCTg
cTACCCGTCGCGGgctcaCCGCCTCGACGAc
    4F+[5]+4F
human, CDKN2A G C ZP10695 ZP10696 TGEK WT MA(2F) NHEJ 21191366 R
ZN0113 cTCACCCGACCCGtgcacGACGCTGCCCGGg
gAGTGGGCTGGGCacgtgCTGCGACGGGCCc
    4F+[5]+4F
human, CDKN2A G C ZP10697 ZP10698 TGEK WT MA(2F) NHEJ 21191366 R
ZN0116 cCCTCAAgCAGGCCCCGCTGgtgcactGAAGAGcCACCCTGTGg
gGGAGTTcGTCCGGGGCGACcacgtgaCTTCTCgGTGGGACACc
    (4F+1+2F)+[7]+(2F+1+3F)
human, CLTA G C ZP10703 ZP10704 LRGS ELD+KKR Sangamo HR 21297641 R
ZN0117 gGGGGGCCTCaCGCACCcgcggcGCAGGAGCTTCAGTGg
cCCCCCGGAGtGCGTGGgcgccgCGTCCTCGAAGTCACc
    (2F+1+3F)+[6]+5F
human, DNM2 G C ZP10705 ZP10706 LRGS ELD+KKR Sangamo HR 21297641 R
ZN0120 tGACCGCTTCTACcccaatGACTTGTGGGTGg
aCTGGCGAAGATGgggttaCTGAACACCCACc
    4F+[6]+4F
human, CXCR4 G C ZP10715 ZP10716 LRGS EL+KK Sangamo NHEJ 21533216 R
ZN0120-T002 cCACCCACAGGTTttaaggGTAGAAGCAGCAa
gGTGGGTGTCCAAaattccCATCTTCGTCGTt
    4F+[6]+4F
human, ADAMTS17, intron G C ZP10715-T002 ZP10716-T002 LRGS EL+KK Sangamo NHEJ 21533216 R Low
ZN0120-T003 cCACCTACTTGTCttgtgtGTTGAAGCAGACa
gGTGGATGAACAGaacacaCAACTTCGTCTGt
    4F+[6]+4F
human, (intergenic) G C ZP10715-T003 ZP10716-T003 LRGS EL+KK Sangamo NHEJ 21533216 R Low
ZN0122 gGACTCCTTCaccaaggGGAGGGGGAg
cCTGAGGAAGtggttccCCTCCCCCTc
    3F+[7]+3F
human, IDUA, intron-exon G C ZP10718 ZP10719 TGPNRGVTK ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0123 gGCCCCCCCCcgccccGCAGATGAGg
cCGGGGGGGGgcggggCGTCTACTCc
    3F+[6]+3F
human, IDUA, intron-exon G C ZP10720 ZP10721 TGGS / LGGS / LRGS ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0124 aCCCTGCGCCccgcgctGCGGCTGCCg
tGGGACGCGGggcgcgaCGCCGACGGc
    3F+[7]+3F
human, IDUA G C ZP10722 ZP10723 TGPNRGVTK ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0125 gCCCCGCCCCttacccGCCGGCGGCc
cGGGGCGGGGaatgggCGGCCGCCGg
    3F+[6]+3F
human, IDUA G C ZP10724 ZP10725 TGGS / LGGS / LRGS ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0126 gCTCAACCTCgcctatGTGGGCGCCg
cGAGTTGGAGcggataCACCCGCGGc
    3F+[6]+3F
human, IDUA G C ZP10726 ZP10727 TGGS / LGGS / LRGS ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0127 cAACCTCGCCtatgtgGGCGCCGTCc
gTTGGAGCGGatacacCCGCGGCAGg
    3F+[6]+3F
human, IDUA G C ZP10728 ZP10729 TGGS / LGGS / LRGS ELD+KKR CoDA HR 21663559 R Low
ZN0130 tCCCCTGCTTGGCtgggcgCAGTGGCTCATGc
aGGGGACGAACCGacccgcGTCACCGAGTACg
    4F+[6]+4F
human, TP53, intron G C ZP10734 ZP10735 QNKK WT, EL+KK MMA NHEJ; HR 21695267 R
ZN0131 cCACCATCCACTAcaactaCATGTGTAACAGt
gGTGGTAGGTGATgttgatGTACACATTGTCa
    4F+[6]+4F
human, TP53 G C ZP10736 ZP10737 QNKK WT, EL+KK MMA NHEJ; HR 21695267 R
ZN0136 gTGCCAGGTACTGTGTcagggtACTAGGGGTATGg
cACGGTCCATGACACAgtcccaTGATCCCCATACc
    5F+[6]+4F
human, F9, intron G C ZP10751 ZP10752 LRGS WT Sangamo NHEJ; HR 21706032 R
ZN0138 gGGAGTGgTGCATGGTGtggcaaCAGGTAaGCTCCATTGTGCtt
cCCTCACcACGTACCACaccgttGTCCATtCGAGGTAACACGaa
    (3F+1+2F)+[6]+(2F+1+4F)
human, SNCA G C ZP10755, ZP10756 ZP10757, ZP10758; ZP10759 LRGS WT, ELD+KKR Sangamo NHEJ; HR 21757228 R
ZN0139 tGTATACATAAAGCTAcaaacatGGAGTTTGCAGTGAAGGTAa
aCATATGTATTTCGATgtttgtaCCTCAAACGTCACTTCCATt
    5F+[7]+(1+6F)
human, RPS6KA3 G C ZPU0026 ZPU0027 (N/A) (N/A) Sangamo HR; HR-DisDel 21765410 R
ZN0140 tGGCTCCTACTCTGTTtgcaagCGATGCATACATGCAAc
aCCGAGGATGAGACAAacgttcGCTACGTATGTACGTTg
    5F+[6]+(1+5F)
human, RPS6KA6 G C ZPU0028 ZPU0029 (N/A) (N/A) Sangamo HR; HR-DisDel 21765410 R
ZNU011 nNNN[x]n(y)gaGATGCAAGATTGCTCAGGGt
nNNN[x]n(y)ctCTACGTTCTAACGAGTCCCa
    xF+[y+2]+(1+6F)
human, IRAK4 G C (N/A) ZPU0030 (N/A) (N/A) Sangamo HR-DisDel 21765410 R
ZN0143 aTACGCCtCGCCCGAGCtgtgcgGTAGGCATTGAGGCAGCCa
tATGCGGaGCGGGCTCGacacgcCATCCGTAACTCCGTCGGt
    (3F+1+2F)+[6]+6F
human, MALAT1, promoter G C ZP10764 ZP10765 LRGS EL+KK Sangamo NHEJ; HR 21844124 R
ZN0144 aGTCTGCCGTTACtgccctGTGGGGCAAGGTGAACGTGg
tCAGACGGCAATGacgggaCACCCCGTTCCACTTGCACc
    4F+[6]+(1+6F)
human, HBB G C ZPU0031 ZPU0032 (N/A) (N/A) Sangamo HR 21881051 R
ZN0145 cCGTTACTGCcctgtGGGGCAAGGt
gGCAATGACGggacaCCCCGTTCCa
    3F+[5]+3F
human, HBB G C ZP10766; ZP10767 (N/A) EL+KK, ELD+KKR OPEN / CoDA NHEJ; HR 21898685 R
ZN0146 aCCTGACTCCtgtggaGAAGTCTGCc
tGGACTGAGGacacctCTTCAGACGg
    3F+[6]+3F
human, HBB G C ZP10070, ZP10771 (N/A) EL+KK, ELD+KKR OPEN / CoDA NHEJ; HR 21898685 R
ZN0148 cCTGTGTGCCCTGAGCCCGctgggGCAGCTGCTGCAGGAc
gGACACACGGGACTCGGGCgacccCGTCGACGACGTCCTg
    6F+[5]+5F
human, SLC16A3 G C ZPU0039 ZPU0040 (N/A) (N/A) Sangamo NHEJ 21930917 R
ZN0149 cCACGGAGCCTCCGGGgctggtGAGGAGCGGGTAGGGGg
gGTGCCTCGGAGGCCCcgaccaCTCCTCGCCCATCCCCc
    5F+[6]+(1+5F)
human, /basigin) G C ZPU0041 ZPU0042 (N/A) (N/A) Sangamo NHEJ 21930917 R
ZN0178 cCTAGCTCCTGGACTGACCactattGGAGCCTGTAAGTATACTGg
gGATCGAGGACCTGACTGGtgataaCCTCGGACATTCATATGACc
    6F+[6]+(1+6F)
human, DMD G C ZPU0043 ZPU0044 (N/A) (N/A) Sangamo NHEJ 21954090 R
ZN0179 cCGCGTCCGCGCCatggcCATCTACAAGCAg
gGCGCAGGCGCGGtaccgGTAGATGTTCGTc
    4F+[5]+4F
human, TP53 G C ZP10845 ZP10846 TGEK Sharkey-AS+R MA(2F) NHEJ 21983922 R
ZN0180 cCCCAACCAGGTAGTagaaggctGTTGTTCAGATATGGCTGTTa
gGGGTTGGTCCATCAtcttccgaCAACAAGTCTATACCGACAAt
    (2+4F)+[8]+(2+6F)
human, C1GALT1C1 G C ZPU0045 ZPU0046 (N/A) (N/A) Sangamo NHEJ 21983924 R
ZN0181 gTGCTGACCATCGacaagaAAGGGActGAAGCTGCTg
cACGACTGGTAGCtgttctTTCCCTgaCTTCGACGAc
    4F+[6]+(2F+2+3F)
human, A1AT G C ZP10847 ZP10848 LRGS WT Sangamo NHEJ; HR 21993621 R
ZN0184 aGACCCACTGagttggGCAAAGGTGGGGc
tCTGGGTGACtcaaccCGTTTCCACCCCg
    3F+[6]+4F
human, F8, intron G C ZPU0047 ZPU0048 (N/A) (N/A) MA NHEJ-Indel/Inversion 22183967 R
ZN0187 tCAAGTCCAGTTCTACgggctcTCGGAGAATGACGAGTGGa
aGTTCAGGTCAAGATGcccgagAGCCTCTTACTGCTCACCt
    5F+[6]+6F
human, TRBC2/TRBC1 G C ZP10856 ZP10857 LRGS WT Sangamo NHEJ 22466705 R
ZN0188 gTGCTGTGGCCTGgAGCAACaaatctGACTTTGCATGTGCAa
cACGACACCGGACcTCGTTGtttagaCTGAAACGTACACGTt
    (2F+1+4F)+[6]+5F
human, TRAC G C ZP10858 ZP10859 LRGS WT Sangamo NHEJ 22466705 R
ZN0201 gGTCGGCCCTACTCAGGACcgtggtCAGGTGAGGCCAGGAGATg
cCAGCCGGGATGAGTCCTGgcaccaGTCCACTCCGGTCCTCTAc
    6F+[6]+6F
human, GALNT2, exon-intron G C ZPU0051 ZPU0052 (N/A) (N/A) Sangamo NHEJ 22566642 R
ZNU016 (N/A)
    
human, TAP2 G C (N/A) (N/A) (N/A) (N/A) Sangamo HR 22875043 R
ZN0209 tTTCTCCCCAGGTGGCTGCgagccTACGAGAGTGCTGTCt
aAAGAGGGGTCCACCGACGctcggATGCTCTCACGACAGa
    6F+[5]+5F
human, PORCN, intron-exon G C ZPU0063 ZPU0064 (N/A) (N/A) Sangamo NHEJ 22888000 R
ZN0213 gCGACGGGGCCCACGCgctgagCCGGCGGTCGGTGCTg
cGCTGCCCCGGGTGCGcgactcGGCCGCCAGCCACGAc
    5F+[6]+5F
human, METTL21D G C ZPU0071 ZPU0072 (N/A) (N/A) Sangamo NHEJ 22948820 R
ZN0219 cACCTCCTCCccggccGGCGGCGGAc
gTGGAGGAGGggccggCCGCCGCCTg
    3F+[6]+3F
human, VEGFA G C ZP10917 - ZP10920 ZP10920 - ZP10923 LRGS D+R OPEN HR 23028823 R
ZN0220 aGTCACCTTCcctgagTGGGCTGGTa
tCAGTGGAAGggactcACCCGACCAt
    3F+[6]+3F
human, GRN, intron G C ZP10924 - ZP10925 ZP10928 - ZP10934 LRGS D+R OPEN HR 23028823 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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