EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
ZFN targeting sequences from zebrafish (Danio rerio): 93 ZFN records match.
(Click on ID to see details)

Note: not including untested EENs targeting endogenous zebrafish genes constructed by an NIH-supported project, which are released in Addgene (list of these
TALENs or ZFNs).


ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0014 gGCCGCACGCTGCacagatGCACAGCAGGTTa
cCGGCGTGCGACGtgtctaCGTGTCGTCCAAt
    4F+[6]+4F
zebrafish, slc24a5 G C ZP10039 ZP10038 LRGS EL+KK Sangamo NHEJ 18500334 R
ZN0015 gTTCATCCAGACGgtgtttGTGGACGTGGGCt
cAAGTAGGTCTGCcacaaaCACCTGCACCCGa
    4F+[6]+4F
zebrafish, slc24a5 G C ZP10041 ZP10040, ZP10042 LRGS EL+KK Sangamo NHEJ 18500334 R
ZN0016 tCTCGACCCTAATgcaatgTACTCGGTCCTGc
aGAGCTGGGATTAcgttacATGAGCCAGGACg
    4F+[6]+4F
zebrafish, ntla G C ZP10043 ZP10044, ZP10045 LRGS WT Sangamo NHEJ 18500334 R
ZN0017 aCACACCTTCagcatgTTGGTGGGAc
tGTGTGGAAGtcgtacAACCACCCTg
    3F+[6]+3F
zebrafish, kdrl G C ZP10046 ZP10047 TGGS D+R PRS NHEJ 18500337 R
ZN0032 gAGCCCCTCCtattgaGATGAAGCTg
cTCGGGGAGGataactCTACTTCGAc
    3F+[6]+3F
zebrafish, tfr2 G C ZP10376 ZP10380 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0033 gGACCACTTCgctgcGGGGACGTCa
cCTGGTGAAGcgacgCCCCTGCAGt
    3F+[5]+3F
zebrafish, dat G C ZP10346 ZP10353 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0034 cTCCATCTACagtcatGAAGAAGCTg
gAGGTAGATGtcagtaCTTCTTCGAc
    3F+[6]+3F
zebrafish, tert G C ZP10363 ZP10368 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0035 aCCCCTACACctgctGCAGGAGGTg
tGGGGATGTGgacgaCGTCCTCCAc
    3F+[5]+3F
zebrafish, hif1ab G C ZP10358 ZP10359 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0036 aTCCTGCTGCgttcctGCAGCAGAGc
tAGGACGACGcaaggaCGTCGTCTCg
    3F+[6]+3F
zebrafish, hey2 G C ZP10385 ZP10388 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0064 cGTCTGCACCtctcaGTGGCGGACc
gCAGACGTGGagagtCACCGCCTGg
    3F+[5]+3F
zebrafish, cxcr4a G C ZP10482 ZP10483 TGGS EL+KK MA & PRS NHEJ 19797767 R
ZN0017B aCACACCTTCagcatgTTGGTGGGAc
tGTGTGGAAGtcgtacAACCACCCTg
    3F+[6]+3F
zebrafish, kdrl G C ZP10048 ZP10552 TGGS D+R, EL+KK PRS & MA NHEJ 20843781 R
ZN0017-T002 aTCCCACCCGagtcctGCAGGTGTGt
tAGGGTGGGCtcaggaCGTCCACACa
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10047-T002 ZP10046-T002 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T003 aCACACCATCctacctTTGGTGGGTa
tGTGTGGTAGgatggaAACCACCCAt
    3F+[6]+3F
zebrafish, -, intron G C ZP10046-T003 ZP10047-T003 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T004 aCACACCTTCacagacGTGGGGGGAa
tGTGTGGAAGtgtctgCACCCCCCTt
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10046 ZP10047-T004 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T005 tCCCGACCAGattgtGAAGGTGTGt
aGGGCTGGTCtaacaCTTCCACACa
    3F+[5]+3F
zebrafish, fhdc1 G C ZP10047-T005 ZP10046 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T006 aACCCACCGAgatacGCGGGTGTGt
tTGGGTGGCTctatgCGCCCACACa
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10047-T006 ZP10046-T004 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T007 aCCCACCCTCgtgatgTTGGAGGGAa
tGGGTGGGAGcactacAACCTCCCTt
    3F+[6]+3F
zebrafish, odf3b G C ZP10046-T005 ZP10047-T007 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T008 cCACACCGGCagactGCGGCGGGAt
gGTGTGGCCGtctgaCGCCGCCCTa
    3F+[5]+3F
zebrafish, tmpoa G C ZP10046-T006 ZP10047-T008 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T009 cCACACCCACaaaagATGGTGGGTc
gGTGTGGGTGttttcTACCACCCAg
    3F+[5]+3F
zebrafish, tanc2, intron G C ZP10046-T007 ZP10047-T009 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T010 cTCCCACCCAggaagtGATGGTGAGt
gAGGGTGGGTccttcaCTACCACTCa
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10047-T010 ZP10046-T008 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T011 aTCCCACCGGagcggtGATGGTGAAc
tAGGGTGGCCtcgccaCTACCACTTg
    3F+[6]+3F
zebrafish, - G C ZP10047-T011 ZP10046-T009 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T012 gTCCCGCCAAcaaatGACGGAGTGc
cAGGGCGGTTgtttaCTGCCTCACg
    3F+[5]+3F
zebrafish, -, intron G C ZP10047-T012 ZP10046-T010 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T013 gCGCACCGCCagacatATGGTGGGAt
cGCGTGGCGGtctgtaTACCACCCTa
    3F+[6]+3F
zebrafish, sox6, intron G C ZP10046-T011 ZP10047-T013 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T014 aTCCCCCCTGccatgaGGAGGTGTGt
tAGGGGGGACggtactCCTCCACACa
    3F+[6]+3F
zebrafish, -, intron G C ZP10047-T014 ZP10046-T012 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T015 cACCCACCCActactGAGGGTGAGc
gTGGGTGGGTgatgaCTCCCACTCg
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10047-T015 ZP10046-T013 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T016 tCACACCTCCaattaGAGGCGGGAt
aGTGTGGAGGttaatCTCCGCCCTa
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10046-T012 ZP10047-T016 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T017 gTCCCTCCCTaagggtGATGGGGTGt
cAGGGAGGGAttcccaCTACCCCACa
    3F+[6]+3F
zebrafish, adcyap1b, 3'-UTR G C ZP10047-T017 ZP10046-T014 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T018 gCACACCAGCtgcattTTGGTGGGTc
cGTGTGGTCGacgtaaAACCACCCAg
    3F+[6]+3F
zebrafish, - G C ZP10046-T015 ZP10047-T003 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T019 cTTCCACCAAgtatcaGAAGGTGTAc
gAAGGTGGTTcatagtCTTCCACATg
    3F+[6]+3F
zebrafish, ctnna2 G C ZP10047-T018 ZP10046-T016 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T020 cTCCCACCAGgatatccgggttacGCAGGTGTGg
gAGGGTGGTCctataggcccaatgCGTCCACACc
    3F+[14]+3F
zebrafish, myo5aa G C ZP10047-T019 ZP10046-T002 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0100 tGCCTTCTCCggggcGCAGAAGGTa
aCGGAAGAGGccccgCGTCTTCCAt
    3F+[5]+3F
zebrafish, actn1 G C ZP10635 ZP10602 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0101 gATCTTCTGCtccaggGGTGAAGGTg
cTAGAAGACGaggtccCCACTTCCAc
    3F+[6]+3F
zebrafish, rag2 G C ZP10601 ZP10655 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0102 cAGCCGCAGCtctcgGCTGTAGACt
gTCGGCGTCGagagcCGACATCTGa
    3F+[5]+3F
zebrafish, gad2 G C ZP10625 ZP10629 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0103 tCTCTTCTCCcccagaGCTGTGGAGa
aGAGAAGAGGgggtctCGACACCTCt
    3F+[6]+3F
zebrafish, lmna G C ZP10633 ZP10630 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0104 gCCCCTCAGCccagaTGGGAGGAGa
cGGGGAGTCGggtctACCCTCCTCt
    3F+[5]+3F
zebrafish, apoeb G C ZP10623 ZP10688 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0105 aCACACCTGCacacaGATGCTGCTg
tGTGTGGACGtgtgtCTACGACGAc
    3F+[5]+3F
zebrafish, trpm7 G C ZP10611 ZP10595 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0106 tGGCCACCTCcaccaGCAGCGGGCc
aCCGGTGGAGgtggtCGTCGCCCGg
    3F+[5]+3F
zebrafish, grip1, intron G C ZP10585 ZP10609 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0107 tCCCCTCTCCtcaaaGCAGATGCAt
aGGGGAGAGGagtttCGTCTACGTa
    3F+[5]+3F
zebrafish, pclo G C ZP10637 ZP10606 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0108 tGGCCCCACCaagcctGCTGGAGGAc
aCCGGGGTGGttcggaCGACCTCCTg
    3F+[6]+3F
zebrafish, jak3 G C ZP10662 ZP10627 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0109 tCTCTGCCGCcacctaGAGGATGGTa
aGAGACGGCGgtggatCTCCTACCAt
    3F+[6]+3F
zebrafish, eif2c1 G C ZP10619 ZP10580 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0110 aGCCCACAGCaatggcGGAGCCGCCt
tCGGGTGTCGttaccgCCTCGGCGGa
    3F+[6]+3F
zebrafish, slitrk1 G C ZP10631 ZP10642 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0111 cGCCGACTGCacagaGAGGAAGTCa
gCGGCTGACGtgtctCTCCTTCAGt
    3F+[5]+3F
zebrafish, bmpr2a G C ZP10576 ZP10612 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0141 cCACATCCCAacagaGCAGCGGGAg
gGTGTAGGGTtgtctCGTCGCCCTc
    3F+[5]+3F
zebrafish, hey2 G C ZP10760 ZP10761 LRGS EL+KK (N/A) NHEJ 21822241 R
ZN0142 tAGCTTCTCAGTCacgcacGCCTGTGAGTTTc
aTCGAAGAGTCAGtgcgtgCGGACACTCAAAg
    4F+[6]+4F
zebrafish, gria3a G C ZP10763 ZP10762 LRGS EL+KK (N/A) NHEJ 21822241 R
ZN0150 aCTCATCCACcagctGGAGATGGCa
tGAGTAGGTGgtcgaCCTCTACCGt
    3F+[5]+3F
zebrafish, ago2 G C ZP10774 ZP10773 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0151 aATCCGTGACggcgcaGTAAGGGCCc
tTAGGCACTGccgcgtCATTCCCGGg
    3F+[6]+3F
zebrafish, arhgef1 G C ZP10775 ZP10776 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0152 aCACCTCCTCctgggtGATGGGGTGg
tGTGGAGGAGgacccaCTACCCCACc
    3F+[6]+3F
zebrafish, braf G C ZP10777 ZP10778 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0153 cCTCCTCCCCagagccGTAGTCGTAc
gGAGGAGGGGtctcggCATCAGCATg
    3F+[6]+3F
zebrafish, chd5 G C ZP10780 ZP10781 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0154 aCCCCTGGTCcggaaGTGTGGGTGt
tGGGGACCAGgccttCACACCCACa
    3F+[5]+3F
zebrafish, col18a1 G C ZP10782 ZP10783 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0155 cCTCAACACCgtcccGCAACGGAGc
gGAGTTGTGGcagggCGTTGCCTCg
    3F+[5]+3F
zebrafish, cxcl12b G C ZP10785 ZP10784 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0064B cGTCTGCACCtctcaGTGGCGGACc
gCAGACGTGGagagtCACCGCCTGg
    3F+[5]+3F
zebrafish, cxcr4a G C ZP10482 ZP10483 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0156 aCTCTCCTTCtccccGAGGGGGTCc
tGAGAGGAAGaggggCTCCCCCAGg
    3F+[5]+3F
zebrafish, dab2 G C ZP10786 ZP10787 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0157 tTTCCTCCACaaagaGGCTGGGATg
aAAGGAGGTGtttctCCGACCCTAc
    3F+[5]+3F
zebrafish, dicer1 G C ZP10789 ZP10790 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0158 tGTCCTCCTCatggcgGTCGATGGTt
aCAGGAGGAGtaccgcCAGCTACCAa
    3F+[6]+3F
zebrafish, drosha G C ZP10791 ZP10792 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0159 cCACGCCCAAgagggGCGGCAGCGg
gGTGCGGGTTctcccCGCCGTCGCc
    3F+[5]+3F
zebrafish, efnb2a G C ZP10794 ZP10793 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0160 aCCCCATTCCccctcaGCAAGAGTGg
tGGGGTAAGGgggagtCGTTCTCACc
    3F+[6]+3F
zebrafish, fli1a G C ZP10798 ZP10797 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0161 cCGCCGCCACagcgggGTGGAGGTGg
gGCGGCGGTGtcgcccCACCTCCACc
    3F+[6]+3F
zebrafish, gata2a G C ZP10802 ZP01801 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0162 cCTCCGCCACagaggaGTCGAGGTGg
gGAGGCGGTGtctcctCAGCTCCACc
    3F+[6]+3F
zebrafish, gata3 G C ZP10803 ZP10804 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0163 cTCCCGCACCagtttgGCATTGGGGt
gAGGGCGTGGtcaaacCGTAACCCCa
    3F+[6]+3F
zebrafish, kif1b G C ZP10805 ZP10806 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0164 aCGCCTCATCgtgacgGTGGAGGAAa
tGCGGAGTAGcactgcCACCTCCTTt
    3F+[6]+3F
zebrafish, kif1b G C ZP10807 ZP10789 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0165 aCTCTCCCCAgggagCAGGCAGCAt
tGAGAGGGGTccctcGTCCGTCGTa
    3F+[5]+3F
zebrafish, klf2a G C ZP10809 ZP10808 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0166 aGCCCTCCAAtctgaaGGTGCAGGAg
tCGGGAGGTTagacttCCACGTCCTc
    3F+[6]+3F
zebrafish, micall2 G C ZP10811 ZP10810 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0167 cACCCTCCTCaggctGTAGAGGAAg
gTGGGAGGAGtccgaCATCTCCTTc
    3F+[5]+3F
zebrafish, nf1a G C ZP10813 ZP10814 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0168 tTTCCGTTACctgtgtGAGGAGGCAg
aAAGGCAATGgacacaCTCCTCCGTc
    3F+[6]+3F
zebrafish, nf1b G C ZP10816 ZP10815 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0169 cCGTTACCTGtgtgagGAGGCAGAAa
gGCAATGGACacactcCTCCGTCTTt
    3F+[6]+3F
zebrafish, nf1b G C ZP10818 ZP10817 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0170 cATCCGCCTGccactGGTGTGGTCg
gTAGGCGGACggtgaCCACACCAGc
    3F+[5]+3F
zebrafish, numbl G C ZP10819 ZP10820 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0171 aCACCTCATCtggacGTGGAGGAGt
tGTGGAGTAGacctgCACCTCCTCa
    3F+[5]+3F
zebrafish, ptpru G C ZP10825 ZP10824 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0172 cCTCCTCCTCttcctCGGGTGGTGg
gGAGGAGGAGaaggaGCCCACCACc
    3F+[5]+3F
zebrafish, sbno2 G C ZP10828 ZP10829 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0173 gGACTACATCaatggaGGAGAGGTGt
cCTGATGTAGttacctCCTCTCCACa
    3F+[6]+3F
zebrafish, sgk1 G C ZP10831 ZP10830 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0174 cCTCCGCCCCttatcaCAGGTGGAGg
gGAGGCGGGGaatagtGTCCACCTCc
    3F+[6]+3F
zebrafish, spon1b G C ZP10834 ZP10835 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0175 tTCCCACCACtcagcAAGTGGGACg
aAGGGTGGTGagtcgTTCACCCTGc
    3F+[5]+3F
zebrafish, tie1 G C ZP10837 ZP10836 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0176 gACCCTCGGCcttcagCAGGTCGTAc
cTGGGAGCCGgaagtcGTCCAGCATg
    3F+[6]+3F
zebrafish, tarbp2 G C ZP10838 ZP10839 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0177 cACACACCTGtgagtcCAGGAGGACg
gTGTGTGGACactcagGTCCTCCTGc
    3F+[6]+3F
zebrafish, yrk G C ZP10841 ZP10842 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0182 aGCCGCCCACgtccatGTTTGAGGAt
tCGGCGGGTGcaggtaCAAACTCCTa
    3F+[6]+3F
zebrafish, kiaa0586 G C ZP10849 ZP10850 LRGS D+R OPEN NHEJ 22028029 R
ZN0190 tGTCCGAGTCcctgtaGACATGGACg
aCAGGCTCAGggacatCTGTACCTGc
    3F+[6]+3F
zebrafish, dab2ipb G C ZP10862 ZP10863 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0191 aAACCATACCgaccgtGGGGAACTGa
tTTGGTATGGctggcaCCCCTTGACt
    3F+[6]+3F
zebrafish, hey2 G C ZP10864 ZP10865 LRGS D+R 2F-MA NHEJ 22543349 R Low
ZN0192 tCGTGCCAGCtgctccGACTCGGCCt
aGCACGGTCGacgaggCTGAGCCGGa
    3F+[6]+3F
zebrafish, rock1 G C ZP10866 ZP10867 LRGS D+R 2F-MA NHEJ 22543349 R No
ZN0193 aCAACTCCAGTTCattttgGACATGGGTACGt
tGTTGAGGTCAAGtaaaacCTGTACCCATGCa
    4F+[6]+4F
zebrafish, zgc:77041, exon-intron G C ZP10868 ZP10869 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0194 cCCATTCCGGCTCtcgggGAAACGGGATCGt
gGGTAAGGCCGAGagcccCTTTGCCCTAGCa
    4F+[5]+4F
zebrafish, dclk2 G C ZP10870 ZP10871 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0195 cTTCATCTGCAGCttggctGTAGCAGACTTGt
gAAGTAGACGTCGaaccgaCATCGTCTGAACa
    4F+[6]+4F
zebrafish, mc4r G C ZP10872 ZP10873 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0196 tGCCTGCCCCGACagcatgGAACTGGGCCCGg
aCGGACGGGGCTGtcgtacCTTGACCCGGGCc
    4F+[6]+4F
zebrafish, lrp8 G C ZP10874 ZP10875 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0197 cCAGCTCCACGTCagcgtaGCTGCATGAAGGg
gGTCGAGGTGCAGtcgcatCGACGTACTTCCc
    4F+[6]+4F
zebrafish, mc3r G C ZP10876 ZP10877 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0198 gAACCTACGCACCtctctGGAGGGCCGTGTg
cTTGGATGCGTGGagagaCCTCCCGGCACAc
    4F+[5]+4F
zebrafish, apoeb G C ZP10878 ZP10879 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0199 cATCCAATTCCTTgcttcaGCAGTGGAAGGTa
gTAGGTTAAGGAAcgaagtCGTCACCTTCCAt
    4F+[6]+4F
zebrafish, lepr, exon-intron G C ZP10880 ZP10881 LRGS D+R 2F-MA NHEJ 22543349 R Low
ZN0200 tTACCATGCCCCTctgtatCAGGAAAAGGTAa
aATGGTACGGGGAgacataGTCCTTTTCCATt
    4F+[6]+4F
zebrafish, zgc:56306 G C ZP10882 ZP10883 LRGS D+R 2F-MA NHEJ 22543349 R Low
ZN0202 gAGCATCACCacatcGAGGACGAGc
cTCGTAGTGGtgtagCTCCTGCTCg
    3F+[5]+3F
zebrafish, myod1 G C ZP10886 ZP10887 (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZNU012 (N/A)
    
zebrafish, cdh15 G C (N/A) (N/A) (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZNU013 (N/A)
    
zebrafish, yap G C (N/A) (N/A) (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZNU014 (N/A)
    
zebrafish, ptenb G C (N/A) (N/A) (N/A) EL+KK CoDA NHEJ 22655075 R
ZNU015 (N/A)
    
zebrafish, tp53 G C (N/A) (N/A) (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZN0211 cTCCTACAGCaaccagGCAGTGGCCt
gAGGATGTCGttggtcCGTCACCGGa
    3F+[6]+3F
zebrafish, foxp2 G C ZPU0067 ZPU0068 (N/A) D+R OPEN NHEJ 22937139 R
ZN0214 tGTCTGCTCCtcagctGGTGATGCTg
aCAGACGAGGagtcgaCCACTACGAc
    3F+[6]+3F
zebrafish, rest G C ZP10901, ZP10902 ZP10903 (N/A) (N/A) (N/A) NHEJ 22951640 R
ZN0215 cAGCTACTCCgacagcGAAGAAGACg
gTCGATGAGGctgtcgCTTCTTCTGc
    3F+[6]+3F
zebrafish, rest G C ZP10905, ZP10906 ZP10907 (N/A) (N/A) (N/A) NHEJ 22951640 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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RSearch EENdb with this reference

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School of Life Sciences, Peking University, Beijing, China
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