EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
CRISPR/Cas targeting sequences from zebrafish (Danio rerio): 22 CRISPR/Cas records match.
(Click on ID to see details)

Note: not including untested EENs targeting endogenous zebrafish genes constructed by an NIH-supported project, which are released in Addgene (list of these
TALENs or ZFNs).


CRISPR/Cas IDWhole SiteSpecies & LocusPAMRNA OligoModif.Ref.Effic.
CN0001 GGTATGTCGGGAACCTCTCCaggga
CCATACAGCCCTTGGAGAGGtccct
    20
zebrafish, tia1l, intron-exon G C NGG gRNA NHEJ 23360964 R
CN0002 GGGACCTGACCGGCCGCAGGaggtc
CCCTGGACTGGCCGGCGTCCtccag
    20
zebrafish, gsk3b G C NGG gRNA NHEJ 23360964 R
CN0003 GGAGCGGTACATGGCGACCGggggc
CCTCGCCATGTACCGCTGGCccccg
    20
zebrafish, fh G C NGG gRNA NHEJ 23360964 R
CN0004 GGAGCGAGCGGAGCGGTACAtggcg
CCTCGCTCGCCTCGCCATGTaccgc
    20
zebrafish, fh G C NGG gRNA NHEJ 23360964 R
CN0005 GGATGCGCGTAAGGAGCGCGaggcg
CCTACGCGCATTCCTCGCGCtccgc
    20
zebrafish, th1 G C NGG gRNA NHEJ 23360964 R
CN0006 GGATGAGCCAAGAAGCCGCTgggaa
CCTACTCGGTTCTTCGGCGAccctt
    20
zebrafish, apoea G C NGG gRNA NHEJ 23360964 R
CN0007 GGAGAAGGTGAAGGACACTGtggtc
CCTCTTCCACTTCCTGTGACaccag
    20
zebrafish, rgs4 G C NGG gRNA NHEJ 23360964 R
CN0008 GGGAAAACACAACCGCAGCCcggcc
CCCTTTTGTGTTGGCGTCGGgccgg
    20
zebrafish, tph1a G C NGG gRNA NHEJ 23360964 R
CN0009 GGAAACTACAGCCCAGCGTCaggcg
CCTTTGATGTCGGGTCGCAGtccgc
    20
zebrafish, drd3 G C NGG gRNA NHEJ 23360964 R
CN0010 GGTGCCGTATCTCTTCTTCAtggtg
CCACGGCATAGAGAAGAAGTaccac
    20
zebrafish, slc6a3 G C NGG gRNA NHEJ 23360964 R No
CN0011 GGTGGTATTTTTTTGAGTGTggggg
CCACCATAAAAAAACTCACAccccc
    20
zebrafish, gria3a, intron G C NGG gRNA NHEJ 23360964 R No
CN0012 GAACTCAGGAGGTTCCTGCtggtt
CTTGAGTCCTCCAAGGACGaccaa
    19
zebrafish, etv2 G C NGG gRNA NHEJ; HR Xiong & Xi group
CN0013 GAATAAGAGAAACCGGGCGGtggat
CTTATTCTCTTTGGCCCGCCaccta
    20
zebrafish, gata5 G C NGG gRNA NHEJ Xiong & Xi group
CN0014 GCTCTGCTGGGGCGCCTGTaggta
CGAGACGACCCCGCGGACAtccat
    19
zebrafish, gata4 G C NGG gRNA NHEJ Xiong & Xi group
CNT01 GGGCCACAACTGTGTCAGTtggca
CCCGGTGTTGACACAGTCAaccgt
    19
zebrafish, dre-mir-126a, 5'-upstream G C NGG gRNA NHEJ; NHEJ-DisDel T1
CNT02 GGCAGTGGGTTTATCTGCGtggac
CCGTCACCCAAATAGACGCacctg
    19
zebrafish, dre-mir-126a, 3'-downstream G C NGG gRNA NHEJ; NHEJ-DisDel T1
CNT03 GAACCAGCTACCTTCTTGTGaggcg
CTTGGTCGATGGAAGAACACtccgc
    20
zebrafish, dre-mir-126a, 5'-upstream G C NGG gRNA NHEJ; NHEJ-DisDel T1
CNT04 GCGTGGACAAACTCAAGACaggtt
CGCACCTGTTTGAGTTCTGtccaa
    19
zebrafish, dre-mir-126a, 3'-downstream G C NGG gRNA NHEJ; NHEJ-DisDel T1
CNT05 GTGGGACAGGTCACGGCTGCtggag
CACCCTGTCCAGTGCCGACGacctc
    20
zebrafish, dre-mir-17a-2 [SNP], 5'-upstream G C NGG gRNA NHEJ; NHEJ-DisDel T1
CNT06 GGGCTGCCCAAACTCAGTCCtggag
CCCGACGGGTTTGAGTCAGGacctc
    20
zebrafish, dre-mir-92a-2, 3'-downstream G C NGG gRNA NHEJ; NHEJ-DisDel T1
CNT07 GGGAAACGCCATTCTGGAGATCctggaa
CCCTTTGCGGTAAGACCTCTAGgacctt
    22
zebrafish, dre-mir-17a-2, 5'-upstream G C NGG gRNA NHEJ; NHEJ-DisDel T1
CNT08 GGGGTTAGAACCTATTTGAaggac
CCCCAATCTTGGATAAACTtcctg
    19
zebrafish, dre-mir-92a-2, 3'-downstream G C NGG gRNA NHEJ; NHEJ-DisDel T1




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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School of Life Sciences, Peking University, Beijing, China
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