EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting sequences from zebrafish (Danio rerio): 93 ZFN, 84 TALEN, 22 CRISPR/Cas records match.
(Click on ID to see details)

Note: not including untested EENs targeting endogenous zebrafish genes constructed by an NIH-supported project, which are released in Addgene (list of these
TALENs or ZFNs).


CRISPR/Cas IDWhole SiteSpecies & LocusPAMRNA OligoModif.Ref.Effic.
CN0001 GGTATGTCGGGAACCTCTCCaggga
CCATACAGCCCTTGGAGAGGtccct
    20
zebrafish, tia1l, intron-exon G C NGG gRNA NHEJ 23360964 R
CN0002 GGGACCTGACCGGCCGCAGGaggtc
CCCTGGACTGGCCGGCGTCCtccag
    20
zebrafish, gsk3b G C NGG gRNA NHEJ 23360964 R
CN0003 GGAGCGGTACATGGCGACCGggggc
CCTCGCCATGTACCGCTGGCccccg
    20
zebrafish, fh G C NGG gRNA NHEJ 23360964 R
CN0004 GGAGCGAGCGGAGCGGTACAtggcg
CCTCGCTCGCCTCGCCATGTaccgc
    20
zebrafish, fh G C NGG gRNA NHEJ 23360964 R
CN0005 GGATGCGCGTAAGGAGCGCGaggcg
CCTACGCGCATTCCTCGCGCtccgc
    20
zebrafish, th1 G C NGG gRNA NHEJ 23360964 R
CN0006 GGATGAGCCAAGAAGCCGCTgggaa
CCTACTCGGTTCTTCGGCGAccctt
    20
zebrafish, apoea G C NGG gRNA NHEJ 23360964 R
CN0007 GGAGAAGGTGAAGGACACTGtggtc
CCTCTTCCACTTCCTGTGACaccag
    20
zebrafish, rgs4 G C NGG gRNA NHEJ 23360964 R
CN0008 GGGAAAACACAACCGCAGCCcggcc
CCCTTTTGTGTTGGCGTCGGgccgg
    20
zebrafish, tph1a G C NGG gRNA NHEJ 23360964 R
CN0009 GGAAACTACAGCCCAGCGTCaggcg
CCTTTGATGTCGGGTCGCAGtccgc
    20
zebrafish, drd3 G C NGG gRNA NHEJ 23360964 R
CN0010 GGTGCCGTATCTCTTCTTCAtggtg
CCACGGCATAGAGAAGAAGTaccac
    20
zebrafish, slc6a3 G C NGG gRNA NHEJ 23360964 R No
CN0011 GGTGGTATTTTTTTGAGTGTggggg
CCACCATAAAAAAACTCACAccccc
    20
zebrafish, gria3a, intron G C NGG gRNA NHEJ 23360964 R No
CN0012 GAACTCAGGAGGTTCCTGCtggtt
CTTGAGTCCTCCAAGGACGaccaa
    19
zebrafish, etv2 G C NGG gRNA NHEJ; HR Xiong & Xi group
CN0013 GAATAAGAGAAACCGGGCGGtggat
CTTATTCTCTTTGGCCCGCCaccta
    20
zebrafish, gata5 G C NGG gRNA NHEJ Xiong & Xi group
CN0014 GCTCTGCTGGGGCGCCTGTaggta
CGAGACGACCCCGCGGACAtccat
    19
zebrafish, gata4 G C NGG gRNA NHEJ Xiong & Xi group
CNT01 GGGCCACAACTGTGTCAGTtggca
CCCGGTGTTGACACAGTCAaccgt
    19
zebrafish, dre-mir-126a, 5'-upstream G C NGG gRNA NHEJ; NHEJ-DisDel T1
CNT02 GGCAGTGGGTTTATCTGCGtggac
CCGTCACCCAAATAGACGCacctg
    19
zebrafish, dre-mir-126a, 3'-downstream G C NGG gRNA NHEJ; NHEJ-DisDel T1
CNT03 GAACCAGCTACCTTCTTGTGaggcg
CTTGGTCGATGGAAGAACACtccgc
    20
zebrafish, dre-mir-126a, 5'-upstream G C NGG gRNA NHEJ; NHEJ-DisDel T1
CNT04 GCGTGGACAAACTCAAGACaggtt
CGCACCTGTTTGAGTTCTGtccaa
    19
zebrafish, dre-mir-126a, 3'-downstream G C NGG gRNA NHEJ; NHEJ-DisDel T1
CNT05 GTGGGACAGGTCACGGCTGCtggag
CACCCTGTCCAGTGCCGACGacctc
    20
zebrafish, dre-mir-17a-2 [SNP], 5'-upstream G C NGG gRNA NHEJ; NHEJ-DisDel T1
CNT06 GGGCTGCCCAAACTCAGTCCtggag
CCCGACGGGTTTGAGTCAGGacctc
    20
zebrafish, dre-mir-92a-2, 3'-downstream G C NGG gRNA NHEJ; NHEJ-DisDel T1
CNT07 GGGAAACGCCATTCTGGAGATCctggaa
CCCTTTGCGGTAAGACCTCTAGgacctt
    22
zebrafish, dre-mir-17a-2, 5'-upstream G C NGG gRNA NHEJ; NHEJ-DisDel T1
CNT08 GGGGTTAGAACCTATTTGAaggac
CCCCAATCTTGGATAAACTtcctg
    19
zebrafish, dre-mir-92a-2, 3'-downstream G C NGG gRNA NHEJ; NHEJ-DisDel T1



TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0013 tATTTCGACGCTCATTCTctggccatggacttcttgAGCATCGGCTTCCGGGa
aTAAAGCTGCGAGTAAGAgaccggtacctgaagaacTCGTAGCCGAAGGCCCt
    17+[18]+16
zebrafish, hey2 G C (std.) +231 WT GG(V&B) 21493687 R
TN0033 tCTTCCGTTTCCACATCCaccacatcccaacagagcAGCGGGAGCAGCAGTAAa
aGAAGGCAAAGGTGTAGGtggtgtagggttgtctcgTCGCCCTCGTCGTCATTt
    17+[18]+17
zebrafish, hey2 G C (others) +63 WT REAL NHEJ 21822241 R
TN0034 tCGTCCAATAGCTTCTcagtcacgcacgcctgtGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAgtcagtgcgtgcggacaCTCAAAGACGAGAAAt
    15+[17]+15
zebrafish, gria3a G C (std.) +63 WT REAL NHEJ 21822241 R
TN0034B tCGTCCAATAGCTTCTcagtcacgcacgcctgTGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAgtcagtgcgtgcggacACTCAAAGACGAGAAAt
    15+[16]+16
zebrafish, gria3a G C (std.) +63 WT REAL NHEJ 21822241 R
TN0034C tCGTCCAATAGCTTCTCagtcacgcacgcctgtGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAGtcagtgcgtgcggacaCTCAAAGACGAGAAAt
    16+[16]+15
zebrafish, gria3a G C (std.) +63 WT REAL NHEJ 21822241 R
TN0035 tGTTATTTTCTCCCCTaaggatcctgcgggcATTTTTGAGCTGGTGGa
aCAATAAAAGAGGGGAttcctaggacgcccgTAAAAACTCGACCACCt
    15+[15]+16
zebrafish, tnikb G C (std.) +63 Sharkey-AS+R UA NHEJ 21822242 R
TN0035B tGTTATTTTCTCCCCTaaggatcctgcgggcATTTTTGAGCTGGTGGa
aCAATAAAAGAGGGGAttcctaggacgcccgTAAAAACTCGACCACCt
    15+[15]+16
zebrafish, tnikb G C (NK) +63 Sharkey-AS+R UA NHEJ 21822242 R
TN0036 tACACACACTCCAGTCTgatggagacagctgATGGTGAGTCACACAAACa
aTGTGTGTGAGGTCAGActacctctgtcgacTACCACTCAGTGTGTTTGt
    16+[14]+18
zebrafish, dip2a G C (std.) +63 Sharkey-AS+R UA NHEJ 21822242 R
TN0146 tGCTCAAACCCGACGAGCatcaccacatcgaggacgAGCACGTGAGGGCGCCCa
aCGAGTTTGGGCTGCTCGtagtggtgtagctcctgcTCGTGCACTCCCGCGGGt
    17+[18]+17
zebrafish, myod1 G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0147 tATTTCTGCGCAGAAGTGgccccaccaagcctgctGGAGGACATACAGAATTa
aTAAAGACGCGTCTTCACcggggtggttcggacgaCCTCCTGTATGTCTTAAt
    17+[17]+17
zebrafish, jak3 G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0145 tCAGCCTTGGTCACATCTcattcagacagtgaaaACATCGGAGGCTTTTCTa
aGTCGGAACCAGTGTAGAgtaagtctgtcactttTGTAGCCTCCGAAAAGAt
    17+[16]+17
zebrafish, phf6 G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0144 tGGAGTGTATGAAAACTGgtcatgtcaggacagaGAAAAGGAGCTTCAGCa
aCCTCACATACTTTTGACcagtacagtcctgtctCTTTTCCTCGAAGTCGt
    17+[16]+16
zebrafish, phf6, ATG G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0142 tTCTTTTCTATCCCATGAcgatgcatcgcacaacAAGGATCAAGATTACAGa
aAGAAAAGATAGGGTACTgctacgtagcgtgttgTTCCTAGTTCTAATGTCt
    17+[16]+17
zebrafish, bmi1a, ATG G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0143 tACCTGCACAGGATGCAgactgaggaggcacagGAAATGTCCCAGATAACa
aTGGACGTGTCCTACGTctgactcctccgtgtcCTTTACAGGGTCTATTGt
    16+[16]+17
zebrafish, ikzf1, ATG G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0150 tACAATACTCCCACTGAAatgacagatgcagacagACTCATGGACAGTTGGTa
aTGTTATGAGGGTGACTTtactgtctacgtctgtcTGAGTACCTGTCAACCAt
    17+[17]+17
zebrafish, epas1b G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0149 tCAGAGCGGCGTAAGGAGaaatctcgtgatgccgCGCGCTGCAGACGGAGTa
aGTCTCGCCGCATTCCTCtttagagcactacggcGCGCGACGTCTGCCTCAt
    17+[16]+17
zebrafish, epas1b G C (std.) +63 WT; ELD+KKR FLASH (Inactive) 22684503 R No
TN0148 tCCGGCAGACATCGTGAAggtggccatcgagtggCCTGGGGCCTTCCCCAAa
aGGCCGTCTGTAGCACTTccaccggtagctcaccGGACCCCGGAAGGGGTTt
    17+[16]+17
zebrafish, elmo1 G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0150-T002 tACGCTACTCCCACTGAAatgacagatgcagacagACTCATGGACAGTTGGTa
aTGCGATGAGGGTGACTTtactgtctacgtctgtcTGAGTACCTGTCAACCAt
    17+[17]+17
zebrafish, epas1b [mutated] G C +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0151 tTTGATGGATCTCTGAGCggcccgcagatctgacAAACTCGCGCTGAAGCGa
aAACTACCTAGAGACTCGccgggcgtctagactgTTTGAGCGCGACTTCGCt
    17+[16]+17
zebrafish, fh G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0152 tACTGGAGTTGTCACTGAaaagaaaaggtagggaTTCCCGTTTTTGCTTGGa
aTGACCTCAACAGTGACTtttcttttccatccctAAGGGCAAAAACGAACCt
    17+[16]+17
zebrafish, hif1ab, exon-intron G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0153 tCGGAGCGCAGGAAGGAGaagtccagggatgcagCGCGATCTCGCAGGGGAa
aGCCTCGCGTCCTTCCTCttcaggtccctacgtcGCGCTAGAGCGTCCCCTt
    17+[16]+17
zebrafish, hif1ab G C (std.) +63 WT; ELD+KKR FLASH (Inactive) 22684503 R No
TN0154 TTCGTTTATACGAGGAACcatgtcgagtgttttAGCGAGAATTTTATTCTA
AAGCAAATATGCTCCTTGgtacagctcacaaaaTCGCTCTTAAAATAAGAT
    18+[15]+18
zebrafish, ptpmt1 G C (std.) +63 WT FLASH (Inactive) 22684503 R No
TN0155 tGGTCCAAAATGAAAAAGtaagaggagtaattacCATGAATGAAGAATATGa
aCCAGGTTTTACTTTTTCattctcctcattaatgGTACTTACTTCTTATACt
    17+[16]+17
zebrafish, ptpmt1 G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0156 TTGAGCAGATCAGATTGGacactgtggacctgacAGGTGTTCCCAGTCTTGA
AACTCGTCTAGTCTAACCtgtgacacctggactgTCCACAAGGGTCAGAACT
    18+[16]+18
zebrafish, ptpmt1 G C (std.) +63 WT FLASH NHEJ 22684503 R
TN0157 tAGAAGAGCGGCATCCCGgcgatcaccatgaagaAGAGATACGGCACCAGGa
aTCTTCTCGCCGTAGGGCcgctagtggtacttctTCTCTATGCCGTGGTCCt
    17+[16]+17
zebrafish, slc6a3 G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0161 tACATGCTGCTGTCCGTctccatcgtgtgtgATGAATATTTTCTGCCa
aTGTACGACGACAGGCAgaggtagcacacacTACTTATAAAAGACGGt
    16+[14]+16
zebrafish, slc24a5 G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0162 tGGACAATTCATCCGGCATccaagcagaggtcagAGGGTGAGAAGGTGCGCa
aCCTGTTAAGTAGGCCGTAggttcgtctccagtcTCCCACTCTTCCACGCGt
    18+[15]+17
zebrafish, ryr3 G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0163 tATTTCAGACGGATGTTtcccagctgcaaagtaACAGTGACAGGACTAAATCCa
aTAAAGTCTGCCTACAAagggtcgacgtttcatTGTCACTGTCCTGATTTAGGt
    16+[16]+20
zebrafish, tbx6l G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0164 tGGACCATTCACCCTGCATccaagcagaggtcagAGGGTGAGAAGGTCAGa
aCCTGGTAAGTGGGACGTAggttcgtctccagtcTCCCACTCTTCCAGTCt
    18+[15]+16
zebrafish, ryr1a G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0165 tGTGAGCACCCAGCGGACCTCCTCTg...cATCTCTGACTGTTGTTCTGATa
aCACTCGTGGGTCGCCTGGAGGAGAc...gTAGAGACTGACAACAAGACTAt
    24+[18]+21
zebrafish, ponzr1 G C (std.) +63-Goldy, +231 WT GG(V&B) NHEJ, HR 23000899 R
TN0166 tTGCAACACTGAGCTCTGTAAACCTg...aACCTCAGGGTCCAGTCGGCAGCAGa
aACGTTGTGACTCGAGACATTTGGAc...tTGGAGTCCCAGGTCAGCCGTCGTCt
    24+[15]+24
zebrafish, crhr1, upstream G C (std.) +63-Goldy, +231 WT GG(V&B) NHEJ 23000899 R
TN0167 tACCCAGAAGACGTTTctgaggaactgattcAGGAGGCCACTCAAAGa
aTGGGTCTTCTGCAAAgactccttgactaagTCCTCCGGTGAGTTTCt
    15+[15]+16
zebrafish, msna G C (std.) +63-Goldy WT GG(V&B) NHEJ 23000899 R
TN0168 tCCACCAGAGAGTAACTacctatttctgggagACTATGTTGACAGAGGCa
aGGTGGTCTCTCATTGAtggataaagaccctcTGATACAACTGTCTCCGt
    16+[15]+17
zebrafish, ppp1cab G C (std.) +63-Goldy WT GG(V&B) NHEJ 23000899 R
TN0169 tCTCCTCAACATACATACTggagagttagttgacaAAGATGAATCATTTGTa
aGAGGAGTTGTATGTATGAcctctcaatcaactgtTTCTACTTAGTAAACAt
    18+[16]+16
zebrafish, cdh5 G C (std.) +63-Goldy WT GG(V&B) NHEJ 23000899 R
TN0170 tGTCAAATCTGCAGCTCCACGCTTc...cCAGAGTCAGAGGCAGAGGa
aCAGTTTAGACGTCGAGGTGCGAAg...gGTCTCAGTCTCCGTCTCCt
    23+[15]+18
zebrafish, crhr2, intron G C (std.) +63-Goldy WT GG(V&B) HR 23000899 R
TN5001 tCCCAAAGATCTTCTTTggcactcggactcataagcAGATCACACAGATTGCTa
aGGGTTTCTAGAAGAAAccgtgagcctgagtattcgTCTAGTGTGTCTAACGAt
    16+[19]+17
zebrafish, brip1 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5002 tCCCAAAGATCTTCTTTggcactcggactcataAGCAGATCACACAGa
aGGGTTTCTAGAAGAAAccgtgagcctgagtatTCGTCTAGTGTGTCt
    16+[16]+14
zebrafish, brip1 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5003 tCTCCCACCATCTGCATggcgtacatcatgaaaacccAGCGGTTGCGTTTGGa
aGAGGGTGGTAGACGTAccgcatgtagtacttttgggTCGCCAACGCAAACCt
    16+[20]+15
zebrafish, dusp5 G C (std.) +63 Sharkey-AS+R UA NHEJ Zhang Group Low
TN5004 tCGTCTCTCCGCTCCAGTccagcgcctggacccccAGCGCCGGGCAATa
aGCAGAGAGGCGAGGTCAggtcgcggacctgggggTCGCGGCCCGTTAt
    17+[17]+13
zebrafish, sema3fb G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5005 tCCTCTGCCTGTCTacaagcgcagactgATGATTGAGCTGGCGa
aGGAGACGGACAGAtgttcgcgtctgacTACTAACTCGACCGCt
    13+[14]+15
zebrafish, pax4 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5006 tGCGATCTCTTAATGACTcaggcagctaaagcaAGTCTGGGAGGCCAGAGa
aCGCTAGAGAATTACTGAgtccgtcgatttcgtTCAGACCCTCCGGTCTCt
    17+[15]+17
zebrafish, fev G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5007 tACATTAAATATCACCTctagggttttactattATTTAAAACTGTATGTa
aTGTAATTTATAGTGGAgatcccaaaatgataaTAAATTTTGACATACAt
    16+[16]+16
zebrafish, dre-mir-126, flanking G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5008 tGGCTCGCTCAATTCATggatggagcccgcAATGAGATAGTATGCa
aCCGAGCGAGTTAAGTAcctacctcgggcgTTACTCTATCATACGt
    16+[13]+15
zebrafish, smo G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5009 tGCCCTTCCTGGTTCAgagaacggttgcgcgGCAGATCAGCCTGGTGGa
aCGGGAAGGACCAAGTctcttgccaacgcgcCGTCTAGTCGGACCACCt
    15+[15]+17
zebrafish, acvr1l G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5010 tAGCCGTGAAGATCTTTtcctctagagatgagAAGTCATGGTTTCGAGa
aTCGGCACTTCTAGAAAaggagatctctactcTTCAGTACCAAAGCTCt
    16+[15]+16
zebrafish, acvr1l G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5011 tCCAGCGAGTCGTCTCTggaggcgtacgcccgctgtcTGCGCATGGGCTGCAGa
aGGTCGCTCAGCAGAGAcctccgcatgcgggcgacagACGCGTACCCGACGTCt
    16+[20]+16
zebrafish, plcg1 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5012 tATCACGGTCACACCCTcaccaccaagatcaagttcAGTGATGTGCTCAACa
aTAGTGCCAGTGTGGGAgtggtggttctagttcaagTCACTACACGAGTTGt
    16+[19]+15
zebrafish, plcg1 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5013 tGCAGGCAGTACATCTTcattaacggaacacAGACGGGCGTGAGGGa
aCGTCCGTCATGTAGAAgtaattgccttgtgTCTGCCCGCACTCCCt
    16+[14]+15
zebrafish, slc22a17 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5014 tCGCCACACATCCTGAGTtcagcaagaagatccAGAGCTTAAAAGCAGCCa
aGCGGTGTGTAGGACTCAagtcgttcttctaggTCTCGAATTTTCGTCGGt
    17+[15]+17
zebrafish, G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5015 tAACGCTAATGCTCCTaaagcatctgatgccaagaccAGCGAGGCTGCCAAAGCa
aTTGCGATTACGAGGAtttcgtagactacggttctggTCGCTCCGACGGTTTCGt
    15+[21]+17
zebrafish, basp1 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5016 tCAATGGCAAGCAGTTccctgtgaccagaggccaAGATGTTGGCAAATa
aGTTACCGTTCGTCAAgggacactggtctccggtTCTACAACCGTTTAt
    15+[18]+14
zebrafish, ssr4 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5017 tTCCGAGTCTACAAATcagtcctgcaggcAGTGCAGAAAACTGa
aAGGCTCAGATGTTTAgtcaggacgtccgTCACGTCTTTTGACt
    15+[13]+14
zebrafish, rtn1a G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5018 tCCTCCAACTGCGGCCCCTgcctcatcccactggAGGAGGATCAAATGGGa
aGGAGGTTGACGCCGGGGAcggagtagggtgaccTCCTCCTAGTTTACCCt
    18+[15]+16
zebrafish, npdc1 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5019 tCTGGCAGCAAACCTgcggctgacgtccgATGGTTCAAAAATGa
aGACCGTCGTTTGGAcgccgactgcaggcTACCAAGTTTTTACt
    14+[14]+14
zebrafish, cadm2a G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5020 tAACCGGATTGAACTggtcagagccacgtcgcAGGAGTTGACGATCAGCa
aTTGGCCTAACTTGAccagtctcggtgcagcgTCCTCAACTGCTAGTCGt
    14+[17]+17
zebrafish, cadm2a G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5021 tCCATGCCCATTACCCTgagctttgcactgAAACCAGGTCTGTGGa
aGGTACGGGTAATGGGActcgaaacgtgacTTTGGTCCAGACACCt
    16+[13]+15
zebrafish, dusp5 G C (std.) +63 Sharkey-AS+R UA NHEJ Zhang Group Low
TN5022 tATAGGGATGCGCTACTggcagccggagcggcttcACACATTGGTGGAAGTGa
aTATCCCTACGCGATGAccgtcggcctcgccgaagTGTGTAACCACCTTCACt
    16+[18]+17
zebrafish, adprhl1 G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5023 tGCTAAAAGAGAAGCTCTggagcaagcgctaagAAATAAGCTGATGGCGGa
aCGATTTTCTCTTCGAGAcctcgttcgcgattcTTTATTCGACTACCGCCt
    17+[15]+17
zebrafish, amotl2b G C (std.) +63 Sharkey-AS+R UA Zhang Group No
TN5024 tGCCAAAAAAGTCTATCTtctcgtttattaatgacgACTTCATTGAAAGGAGa
aCGGTTTTTTCAGATAGAagagcaaataattactgcTGAAGTAACTTTCCTCt
    17+[18]+16
zebrafish, snx11 G C (std.) +63 Sharkey-AS+R UA NHEJ Zhang Group Low
TNT01 tGGTAATTTCATCCGTCTgatcgagccatggaacAGGACGCACTTATATa
aCCATTAAAGTAGGCAGActagctcggtaccttgTCCTGCGTGAATATAt
    17+[16]+15
zebrafish, sema3fb, exon 4 G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT02 tGTCCTTTTAACACATaaagatgatatcatACACAGTATTTATTGTa
aCAGGAAAATTGTGTAtttctactatagtaTGTGTCATAAATAACAt
    15+[14]+16
zebrafish, sema3fb, exon 18 G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT03 tATAGTTGAAACCTGATtaacttagtaaaatAACTGCATGGTGGTGCa
aTATCAACTTTGGACTAattgaatcattttaTTGACGTACCACCACGt
    16+[14]+16
zebrafish, dre-mir-126a, 5'-upstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT04 tGGACCCACTGGCATCTgacttgtttaattttAGAGGTTAAAGGAAATa
aCCTGGGTGACCGTAGActgaacaaattaaaaTCTCCAATTTCCTTTAt
    16+[15]+16
zebrafish, dre-mir-126a, 3'-downstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT05 tGGACCCACTGGCATCTgacttgtttaattttAGAGGTTAAAGGaaata
aCCTGGGTGACCGTAGActgaacaaattaaaaTCTCCAATTTCCtttat
    16+[15]+12
zebrafish, dre-mir-126a, 3'-downstream G C (std.) +63 Sharkey-AS+R UA NHEJ T1
TNT06 tATCTGCGTGGACAAACTcaagacaggtttggatgcAGATCAGTGTTATCGa
aTAGACGCACCTGTTTGAgttctgtccaaacctacgTCTAGTCACAATAGCt
    17+[18]+15
zebrafish, dre-mir-126a, 3'-downstream G C (std.) +63 Sharkey-AS+R UA NHEJ T1
TNT07 tACAGAATCTGCATTGTataagcgcaatcgaattgATTGTGGGTGTGTTATa
aTGTCTTAGACGTAACAtattcgcgttagcttaacTAACACCCACACAATAt
    16+[18]+16
zebrafish, dre-mir-126b, 5'-upstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT08 tGACTGGTGAACACAACTataacctacagactgAGCACGTCCGACTGCATa
aCTGACCACTTGTGTTGAtattggatgtctgacTCGTGCAGGCTGACGTAt
    17+[15]+17
zebrafish, dre-mir-126b, 3'-downstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT09 tATCATGAGCTCGTCGCTcgttgacgtaaatgcATTGTTGATTTCATa
aTAGTACTCGAGCAGCGAgcaactgcatttacgTAACAACTAAAGTAt
    17+[15]+14
zebrafish, dre-mir-17a-1 [SNP], 5'-upstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT10 tGCACTTGTCCCGGCCTgtaaaggattgtggagATTGTACCCCACAAATa
aCGTGAACAGGGCCGGAcatttcctaacacctcTAACATGGGGTGTTTAt
    16+[16]+16
zebrafish, dre-mir-92a-1, 3'-downstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT11 tAGTCCTCTCCACCTccatagttggggagaATGTGGGAGATTTGa
aTCAGGAGAGGTGGAggtatcaacccctctTACACCCTCTAAACt
    14+[15]+14
zebrafish, dre-mir-17a-1, 5'-upstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT12 tATCCACTTAATTACTGTgtattagatctgatATCAACTGAACAGGGTa
aTAGGTGAATTAATGACAcataatctagactaTAGTTGACTTGTCCCAt
    17+[14]+16
zebrafish, dre-mir-92a-1, 3'-downstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT13 tGTCCCACGTCATGTcactgtagtgtcaaAGTGCTTACAGTGCa
aCAGGGTGCAGTACAgtgacatcacagttTCACGAATGTCACGt
    14+[14]+14
zebrafish, dre-mir-17a-2, 5'-upstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT14 tCTAACATCCTTACAGGTgtgttgaggcaagttagAGCTAAAGTCTGCTGGa
aGATTGTAGGAATGTCCAcacaactccgttcaatcTCGATTTCAGACGACCt
    17+[17]+16
zebrafish, dre-mir-92a-2, 3'-downstream G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT15 tGAGCCCTACTCTGTCATtgaggtggtgccaagtgATTTGATGTGCACTGCTGa
aCTCGGGATGAGACAGTAactccaccacggttcacTAAACTACACGTGACGACt
    17+[17]+18
zebrafish, fgd5 G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT16 tACATCTCCAACTATGATcgcagcatgtcagtgctggACGAGAGCTGTAGAAAGa
aTGTAGAGGTTGATACTAgcgtcgtacagtcacgaccTGCTCTCGACATCTTTCt
    17+[19]+17
zebrafish, G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT17 tGCAGCTACACCTTCATcctgcccgagatggagAGCTGTCAGGAGCAGa
aCGTCGATGTGGAAGTAggacgggctctacctcTCGACAGTCCTCGTCt
    16+[16]+15
zebrafish, [unnamed] C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT18 tCTCAGGATGCTCTGATTtgttttgtacactgggaAATAGTGAAAAGTGGCTa
aGAGTCCTACGAGACTAAacaaaacatgtgaccctTTATCACTTTTCACCGAt
    17+[17]+17
zebrafish, psmf1 G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT19 tTTACATTCATCCAGACtcgcctgcttccggtgaAACGTGGATGCGCCAa
aAATGTAAGTAGGTCTGagcggacgaaggccactTTGCACCTACGCGGTt
    16+[17]+15
zebrafish, tbx18 G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT20 tCTTCTGAAATTCGGTtccggtaaagacccggggaCATCAAACGAGAGCa
aGAAGACTTTAAGCCAaggccatttctgggcccctGTAGTTTGCTCTCGt
    15+[19]+14
zebrafish, tcf21 G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT21 tCACACCGGAGCGCCAtacggctcgctggcccaGCACTCCTTCATCAa
aGTGTGGCCTCGCGGTatgccgagcgaccgggtCGTGAGGAAGTAGTt
    15+[17]+14
zebrafish, wt1a G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT22 tGGCCTTACACTATCCATgacctcaggaaccttggAGCCAAGAAGTTTTATGGa
aCCGGAATGTGATAGGTActggagtccttggaaccTCGGTTCTTCAAAATACCt
    17+[17]+18
zebrafish, mical2b G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT23 tGTCCCGCAGTGCACTgacggagattgttgaccAGGAGGCAGCTTATCTGGa
aCAGGGCGTCACGTGActgcctctaacaactggTCCTCCGTCGAATAGACCt
    15+[17]+18
zebrafish, gtf3c3 G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT24 tCCACCTGCACCTCAGGTcctgcagagatcaaactccATTCAGCAGATGGAGCa
aGGTGGACGTGGAGTCCAggacgtctctagtttgaggTAAGTCGTCTACCTCGt
    17+[19]+16
zebrafish, [unnamed] C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1
TNT25 tCTAAGCTCTCCCTGTccattctagacgcctgAGTTCAGGAAAACTTCa
aGATTCGAGAGGGACAggtaagatctgcggacTCAAGTCCTTTTGAAGt
    15+[16]+16
zebrafish, midn G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0014 gGCCGCACGCTGCacagatGCACAGCAGGTTa
cCGGCGTGCGACGtgtctaCGTGTCGTCCAAt
    4F+[6]+4F
zebrafish, slc24a5 G C ZP10039 ZP10038 LRGS EL+KK Sangamo NHEJ 18500334 R
ZN0015 gTTCATCCAGACGgtgtttGTGGACGTGGGCt
cAAGTAGGTCTGCcacaaaCACCTGCACCCGa
    4F+[6]+4F
zebrafish, slc24a5 G C ZP10041 ZP10040, ZP10042 LRGS EL+KK Sangamo NHEJ 18500334 R
ZN0016 tCTCGACCCTAATgcaatgTACTCGGTCCTGc
aGAGCTGGGATTAcgttacATGAGCCAGGACg
    4F+[6]+4F
zebrafish, ntla G C ZP10043 ZP10044, ZP10045 LRGS WT Sangamo NHEJ 18500334 R
ZN0017 aCACACCTTCagcatgTTGGTGGGAc
tGTGTGGAAGtcgtacAACCACCCTg
    3F+[6]+3F
zebrafish, kdrl G C ZP10046 ZP10047 TGGS D+R PRS NHEJ 18500337 R
ZN0032 gAGCCCCTCCtattgaGATGAAGCTg
cTCGGGGAGGataactCTACTTCGAc
    3F+[6]+3F
zebrafish, tfr2 G C ZP10376 ZP10380 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0033 gGACCACTTCgctgcGGGGACGTCa
cCTGGTGAAGcgacgCCCCTGCAGt
    3F+[5]+3F
zebrafish, dat G C ZP10346 ZP10353 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0034 cTCCATCTACagtcatGAAGAAGCTg
gAGGTAGATGtcagtaCTTCTTCGAc
    3F+[6]+3F
zebrafish, tert G C ZP10363 ZP10368 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0035 aCCCCTACACctgctGCAGGAGGTg
tGGGGATGTGgacgaCGTCCTCCAc
    3F+[5]+3F
zebrafish, hif1ab G C ZP10358 ZP10359 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0036 aTCCTGCTGCgttcctGCAGCAGAGc
tAGGACGACGcaaggaCGTCGTCTCg
    3F+[6]+3F
zebrafish, hey2 G C ZP10385 ZP10388 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0064 cGTCTGCACCtctcaGTGGCGGACc
gCAGACGTGGagagtCACCGCCTGg
    3F+[5]+3F
zebrafish, cxcr4a G C ZP10482 ZP10483 TGGS EL+KK MA & PRS NHEJ 19797767 R
ZN0017B aCACACCTTCagcatgTTGGTGGGAc
tGTGTGGAAGtcgtacAACCACCCTg
    3F+[6]+3F
zebrafish, kdrl G C ZP10048 ZP10552 TGGS D+R, EL+KK PRS & MA NHEJ 20843781 R
ZN0017-T002 aTCCCACCCGagtcctGCAGGTGTGt
tAGGGTGGGCtcaggaCGTCCACACa
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10047-T002 ZP10046-T002 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T003 aCACACCATCctacctTTGGTGGGTa
tGTGTGGTAGgatggaAACCACCCAt
    3F+[6]+3F
zebrafish, -, intron G C ZP10046-T003 ZP10047-T003 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T004 aCACACCTTCacagacGTGGGGGGAa
tGTGTGGAAGtgtctgCACCCCCCTt
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10046 ZP10047-T004 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T005 tCCCGACCAGattgtGAAGGTGTGt
aGGGCTGGTCtaacaCTTCCACACa
    3F+[5]+3F
zebrafish, fhdc1 G C ZP10047-T005 ZP10046 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T006 aACCCACCGAgatacGCGGGTGTGt
tTGGGTGGCTctatgCGCCCACACa
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10047-T006 ZP10046-T004 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T007 aCCCACCCTCgtgatgTTGGAGGGAa
tGGGTGGGAGcactacAACCTCCCTt
    3F+[6]+3F
zebrafish, odf3b G C ZP10046-T005 ZP10047-T007 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T008 cCACACCGGCagactGCGGCGGGAt
gGTGTGGCCGtctgaCGCCGCCCTa
    3F+[5]+3F
zebrafish, tmpoa G C ZP10046-T006 ZP10047-T008 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T009 cCACACCCACaaaagATGGTGGGTc
gGTGTGGGTGttttcTACCACCCAg
    3F+[5]+3F
zebrafish, tanc2, intron G C ZP10046-T007 ZP10047-T009 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T010 cTCCCACCCAggaagtGATGGTGAGt
gAGGGTGGGTccttcaCTACCACTCa
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10047-T010 ZP10046-T008 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T011 aTCCCACCGGagcggtGATGGTGAAc
tAGGGTGGCCtcgccaCTACCACTTg
    3F+[6]+3F
zebrafish, - G C ZP10047-T011 ZP10046-T009 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T012 gTCCCGCCAAcaaatGACGGAGTGc
cAGGGCGGTTgtttaCTGCCTCACg
    3F+[5]+3F
zebrafish, -, intron G C ZP10047-T012 ZP10046-T010 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T013 gCGCACCGCCagacatATGGTGGGAt
cGCGTGGCGGtctgtaTACCACCCTa
    3F+[6]+3F
zebrafish, sox6, intron G C ZP10046-T011 ZP10047-T013 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T014 aTCCCCCCTGccatgaGGAGGTGTGt
tAGGGGGGACggtactCCTCCACACa
    3F+[6]+3F
zebrafish, -, intron G C ZP10047-T014 ZP10046-T012 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T015 cACCCACCCActactGAGGGTGAGc
gTGGGTGGGTgatgaCTCCCACTCg
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10047-T015 ZP10046-T013 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T016 tCACACCTCCaattaGAGGCGGGAt
aGTGTGGAGGttaatCTCCGCCCTa
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10046-T012 ZP10047-T016 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T017 gTCCCTCCCTaagggtGATGGGGTGt
cAGGGAGGGAttcccaCTACCCCACa
    3F+[6]+3F
zebrafish, adcyap1b, 3'-UTR G C ZP10047-T017 ZP10046-T014 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T018 gCACACCAGCtgcattTTGGTGGGTc
cGTGTGGTCGacgtaaAACCACCCAg
    3F+[6]+3F
zebrafish, - G C ZP10046-T015 ZP10047-T003 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T019 cTTCCACCAAgtatcaGAAGGTGTAc
gAAGGTGGTTcatagtCTTCCACATg
    3F+[6]+3F
zebrafish, ctnna2 G C ZP10047-T018 ZP10046-T016 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T020 cTCCCACCAGgatatccgggttacGCAGGTGTGg
gAGGGTGGTCctataggcccaatgCGTCCACACc
    3F+[14]+3F
zebrafish, myo5aa G C ZP10047-T019 ZP10046-T002 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0100 tGCCTTCTCCggggcGCAGAAGGTa
aCGGAAGAGGccccgCGTCTTCCAt
    3F+[5]+3F
zebrafish, actn1 G C ZP10635 ZP10602 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0101 gATCTTCTGCtccaggGGTGAAGGTg
cTAGAAGACGaggtccCCACTTCCAc
    3F+[6]+3F
zebrafish, rag2 G C ZP10601 ZP10655 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0102 cAGCCGCAGCtctcgGCTGTAGACt
gTCGGCGTCGagagcCGACATCTGa
    3F+[5]+3F
zebrafish, gad2 G C ZP10625 ZP10629 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0103 tCTCTTCTCCcccagaGCTGTGGAGa
aGAGAAGAGGgggtctCGACACCTCt
    3F+[6]+3F
zebrafish, lmna G C ZP10633 ZP10630 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0104 gCCCCTCAGCccagaTGGGAGGAGa
cGGGGAGTCGggtctACCCTCCTCt
    3F+[5]+3F
zebrafish, apoeb G C ZP10623 ZP10688 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0105 aCACACCTGCacacaGATGCTGCTg
tGTGTGGACGtgtgtCTACGACGAc
    3F+[5]+3F
zebrafish, trpm7 G C ZP10611 ZP10595 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0106 tGGCCACCTCcaccaGCAGCGGGCc
aCCGGTGGAGgtggtCGTCGCCCGg
    3F+[5]+3F
zebrafish, grip1, intron G C ZP10585 ZP10609 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0107 tCCCCTCTCCtcaaaGCAGATGCAt
aGGGGAGAGGagtttCGTCTACGTa
    3F+[5]+3F
zebrafish, pclo G C ZP10637 ZP10606 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0108 tGGCCCCACCaagcctGCTGGAGGAc
aCCGGGGTGGttcggaCGACCTCCTg
    3F+[6]+3F
zebrafish, jak3 G C ZP10662 ZP10627 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0109 tCTCTGCCGCcacctaGAGGATGGTa
aGAGACGGCGgtggatCTCCTACCAt
    3F+[6]+3F
zebrafish, eif2c1 G C ZP10619 ZP10580 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0110 aGCCCACAGCaatggcGGAGCCGCCt
tCGGGTGTCGttaccgCCTCGGCGGa
    3F+[6]+3F
zebrafish, slitrk1 G C ZP10631 ZP10642 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0111 cGCCGACTGCacagaGAGGAAGTCa
gCGGCTGACGtgtctCTCCTTCAGt
    3F+[5]+3F
zebrafish, bmpr2a G C ZP10576 ZP10612 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0141 cCACATCCCAacagaGCAGCGGGAg
gGTGTAGGGTtgtctCGTCGCCCTc
    3F+[5]+3F
zebrafish, hey2 G C ZP10760 ZP10761 LRGS EL+KK (N/A) NHEJ 21822241 R
ZN0142 tAGCTTCTCAGTCacgcacGCCTGTGAGTTTc
aTCGAAGAGTCAGtgcgtgCGGACACTCAAAg
    4F+[6]+4F
zebrafish, gria3a G C ZP10763 ZP10762 LRGS EL+KK (N/A) NHEJ 21822241 R
ZN0150 aCTCATCCACcagctGGAGATGGCa
tGAGTAGGTGgtcgaCCTCTACCGt
    3F+[5]+3F
zebrafish, ago2 G C ZP10774 ZP10773 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0151 aATCCGTGACggcgcaGTAAGGGCCc
tTAGGCACTGccgcgtCATTCCCGGg
    3F+[6]+3F
zebrafish, arhgef1 G C ZP10775 ZP10776 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0152 aCACCTCCTCctgggtGATGGGGTGg
tGTGGAGGAGgacccaCTACCCCACc
    3F+[6]+3F
zebrafish, braf G C ZP10777 ZP10778 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0153 cCTCCTCCCCagagccGTAGTCGTAc
gGAGGAGGGGtctcggCATCAGCATg
    3F+[6]+3F
zebrafish, chd5 G C ZP10780 ZP10781 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0154 aCCCCTGGTCcggaaGTGTGGGTGt
tGGGGACCAGgccttCACACCCACa
    3F+[5]+3F
zebrafish, col18a1 G C ZP10782 ZP10783 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0155 cCTCAACACCgtcccGCAACGGAGc
gGAGTTGTGGcagggCGTTGCCTCg
    3F+[5]+3F
zebrafish, cxcl12b G C ZP10785 ZP10784 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0064B cGTCTGCACCtctcaGTGGCGGACc
gCAGACGTGGagagtCACCGCCTGg
    3F+[5]+3F
zebrafish, cxcr4a G C ZP10482 ZP10483 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0156 aCTCTCCTTCtccccGAGGGGGTCc
tGAGAGGAAGaggggCTCCCCCAGg
    3F+[5]+3F
zebrafish, dab2 G C ZP10786 ZP10787 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0157 tTTCCTCCACaaagaGGCTGGGATg
aAAGGAGGTGtttctCCGACCCTAc
    3F+[5]+3F
zebrafish, dicer1 G C ZP10789 ZP10790 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0158 tGTCCTCCTCatggcgGTCGATGGTt
aCAGGAGGAGtaccgcCAGCTACCAa
    3F+[6]+3F
zebrafish, drosha G C ZP10791 ZP10792 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0159 cCACGCCCAAgagggGCGGCAGCGg
gGTGCGGGTTctcccCGCCGTCGCc
    3F+[5]+3F
zebrafish, efnb2a G C ZP10794 ZP10793 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0160 aCCCCATTCCccctcaGCAAGAGTGg
tGGGGTAAGGgggagtCGTTCTCACc
    3F+[6]+3F
zebrafish, fli1a G C ZP10798 ZP10797 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0161 cCGCCGCCACagcgggGTGGAGGTGg
gGCGGCGGTGtcgcccCACCTCCACc
    3F+[6]+3F
zebrafish, gata2a G C ZP10802 ZP01801 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0162 cCTCCGCCACagaggaGTCGAGGTGg
gGAGGCGGTGtctcctCAGCTCCACc
    3F+[6]+3F
zebrafish, gata3 G C ZP10803 ZP10804 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0163 cTCCCGCACCagtttgGCATTGGGGt
gAGGGCGTGGtcaaacCGTAACCCCa
    3F+[6]+3F
zebrafish, kif1b G C ZP10805 ZP10806 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0164 aCGCCTCATCgtgacgGTGGAGGAAa
tGCGGAGTAGcactgcCACCTCCTTt
    3F+[6]+3F
zebrafish, kif1b G C ZP10807 ZP10789 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0165 aCTCTCCCCAgggagCAGGCAGCAt
tGAGAGGGGTccctcGTCCGTCGTa
    3F+[5]+3F
zebrafish, klf2a G C ZP10809 ZP10808 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0166 aGCCCTCCAAtctgaaGGTGCAGGAg
tCGGGAGGTTagacttCCACGTCCTc
    3F+[6]+3F
zebrafish, micall2 G C ZP10811 ZP10810 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0167 cACCCTCCTCaggctGTAGAGGAAg
gTGGGAGGAGtccgaCATCTCCTTc
    3F+[5]+3F
zebrafish, nf1a G C ZP10813 ZP10814 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0168 tTTCCGTTACctgtgtGAGGAGGCAg
aAAGGCAATGgacacaCTCCTCCGTc
    3F+[6]+3F
zebrafish, nf1b G C ZP10816 ZP10815 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0169 cCGTTACCTGtgtgagGAGGCAGAAa
gGCAATGGACacactcCTCCGTCTTt
    3F+[6]+3F
zebrafish, nf1b G C ZP10818 ZP10817 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0170 cATCCGCCTGccactGGTGTGGTCg
gTAGGCGGACggtgaCCACACCAGc
    3F+[5]+3F
zebrafish, numbl G C ZP10819 ZP10820 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0171 aCACCTCATCtggacGTGGAGGAGt
tGTGGAGTAGacctgCACCTCCTCa
    3F+[5]+3F
zebrafish, ptpru G C ZP10825 ZP10824 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0172 cCTCCTCCTCttcctCGGGTGGTGg
gGAGGAGGAGaaggaGCCCACCACc
    3F+[5]+3F
zebrafish, sbno2 G C ZP10828 ZP10829 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0173 gGACTACATCaatggaGGAGAGGTGt
cCTGATGTAGttacctCCTCTCCACa
    3F+[6]+3F
zebrafish, sgk1 G C ZP10831 ZP10830 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0174 cCTCCGCCCCttatcaCAGGTGGAGg
gGAGGCGGGGaatagtGTCCACCTCc
    3F+[6]+3F
zebrafish, spon1b G C ZP10834 ZP10835 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0175 tTCCCACCACtcagcAAGTGGGACg
aAGGGTGGTGagtcgTTCACCCTGc
    3F+[5]+3F
zebrafish, tie1 G C ZP10837 ZP10836 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0176 gACCCTCGGCcttcagCAGGTCGTAc
cTGGGAGCCGgaagtcGTCCAGCATg
    3F+[6]+3F
zebrafish, tarbp2 G C ZP10838 ZP10839 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0177 cACACACCTGtgagtcCAGGAGGACg
gTGTGTGGACactcagGTCCTCCTGc
    3F+[6]+3F
zebrafish, yrk G C ZP10841 ZP10842 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0182 aGCCGCCCACgtccatGTTTGAGGAt
tCGGCGGGTGcaggtaCAAACTCCTa
    3F+[6]+3F
zebrafish, kiaa0586 G C ZP10849 ZP10850 LRGS D+R OPEN NHEJ 22028029 R
ZN0190 tGTCCGAGTCcctgtaGACATGGACg
aCAGGCTCAGggacatCTGTACCTGc
    3F+[6]+3F
zebrafish, dab2ipb G C ZP10862 ZP10863 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0191 aAACCATACCgaccgtGGGGAACTGa
tTTGGTATGGctggcaCCCCTTGACt
    3F+[6]+3F
zebrafish, hey2 G C ZP10864 ZP10865 LRGS D+R 2F-MA NHEJ 22543349 R Low
ZN0192 tCGTGCCAGCtgctccGACTCGGCCt
aGCACGGTCGacgaggCTGAGCCGGa
    3F+[6]+3F
zebrafish, rock1 G C ZP10866 ZP10867 LRGS D+R 2F-MA NHEJ 22543349 R No
ZN0193 aCAACTCCAGTTCattttgGACATGGGTACGt
tGTTGAGGTCAAGtaaaacCTGTACCCATGCa
    4F+[6]+4F
zebrafish, zgc:77041, exon-intron G C ZP10868 ZP10869 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0194 cCCATTCCGGCTCtcgggGAAACGGGATCGt
gGGTAAGGCCGAGagcccCTTTGCCCTAGCa
    4F+[5]+4F
zebrafish, dclk2 G C ZP10870 ZP10871 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0195 cTTCATCTGCAGCttggctGTAGCAGACTTGt
gAAGTAGACGTCGaaccgaCATCGTCTGAACa
    4F+[6]+4F
zebrafish, mc4r G C ZP10872 ZP10873 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0196 tGCCTGCCCCGACagcatgGAACTGGGCCCGg
aCGGACGGGGCTGtcgtacCTTGACCCGGGCc
    4F+[6]+4F
zebrafish, lrp8 G C ZP10874 ZP10875 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0197 cCAGCTCCACGTCagcgtaGCTGCATGAAGGg
gGTCGAGGTGCAGtcgcatCGACGTACTTCCc
    4F+[6]+4F
zebrafish, mc3r G C ZP10876 ZP10877 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0198 gAACCTACGCACCtctctGGAGGGCCGTGTg
cTTGGATGCGTGGagagaCCTCCCGGCACAc
    4F+[5]+4F
zebrafish, apoeb G C ZP10878 ZP10879 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0199 cATCCAATTCCTTgcttcaGCAGTGGAAGGTa
gTAGGTTAAGGAAcgaagtCGTCACCTTCCAt
    4F+[6]+4F
zebrafish, lepr, exon-intron G C ZP10880 ZP10881 LRGS D+R 2F-MA NHEJ 22543349 R Low
ZN0200 tTACCATGCCCCTctgtatCAGGAAAAGGTAa
aATGGTACGGGGAgacataGTCCTTTTCCATt
    4F+[6]+4F
zebrafish, zgc:56306 G C ZP10882 ZP10883 LRGS D+R 2F-MA NHEJ 22543349 R Low
ZN0202 gAGCATCACCacatcGAGGACGAGc
cTCGTAGTGGtgtagCTCCTGCTCg
    3F+[5]+3F
zebrafish, myod1 G C ZP10886 ZP10887 (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZNU012 (N/A)
    
zebrafish, cdh15 G C (N/A) (N/A) (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZNU013 (N/A)
    
zebrafish, yap G C (N/A) (N/A) (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZNU014 (N/A)
    
zebrafish, ptenb G C (N/A) (N/A) (N/A) EL+KK CoDA NHEJ 22655075 R
ZNU015 (N/A)
    
zebrafish, tp53 G C (N/A) (N/A) (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZN0211 cTCCTACAGCaaccagGCAGTGGCCt
gAGGATGTCGttggtcCGTCACCGGa
    3F+[6]+3F
zebrafish, foxp2 G C ZPU0067 ZPU0068 (N/A) D+R OPEN NHEJ 22937139 R
ZN0214 tGTCTGCTCCtcagctGGTGATGCTg
aCAGACGAGGagtcgaCCACTACGAc
    3F+[6]+3F
zebrafish, rest G C ZP10901, ZP10902 ZP10903 (N/A) (N/A) (N/A) NHEJ 22951640 R
ZN0215 cAGCTACTCCgacagcGAAGAAGACg
gTCGATGAGGctgtcgCTTCTTCTGc
    3F+[6]+3F
zebrafish, rest G C ZP10905, ZP10906 ZP10907 (N/A) (N/A) (N/A) NHEJ 22951640 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
GSearch EENdb with this gene symbol
RSearch EENdb with this reference

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School of Life Sciences, Peking University, Beijing, China
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