EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting sequences from cattle (Bos taurus): 3 ZFN, 8 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0181 tCTGCGCTCCTTCAAAGTgtttaagatcaagcctgAGAAGAGGTGGCAGGACa
aGACGCGAGGAAGTTTCAcaaattctagttcggacTCTTCTCCACCGTCCTGt
    17+[17]+17
cow, GGTA1 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0184 tCCTTTCCTCCAGGGATCCCTc...cACTGGAGAAGCAACAGAGGAGCa
aGGAAAGGAGGTCCCTAGGGAg...gTGACCTCTTCGTTGTCTCCTCGt
    20+[15]+22
cow, ACAN G (std.) +63-Goldy, +231 WT GG(V&B) NHEJ 23027955 R
TN0185 tGCAATCCCAGGCTTCACCGTTg...gACGGAATGGGAACTTGCCTACa
aCGTTAGGGTCCGAAGTGGCAAc...cTGCCTTACCCTTGAACGGATGt
    21+[15]+21
cow, ACAN, intron-exon G (std.) +63-Goldy, +231 WT GG(V&B) 23027955 R Low
TN0199A tGTGATGAACACTCCACAGAATCTc...cCTCTAACTGTGGATTTTGAa
aCACTACTTGTGAGGTGTCTTAGAg...gGAGATTGACACCTAAAACTt
    23+[16]+19
cow, MSTN G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0199B tGTGATGAACACTCCACAGAATCTc...aACTGTGGATTTTGAAGCTTTTGGa
aCACTACTTGTGAGGTGTCTTAGAg...tTGACACCTAAAACTTCGAAAACCt
    23+[21]+23
cow, MSTN G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0200 tGCAAGAAGCGACCAAAACCTggaggaggatggaacACTGGGGGGAGCCGATa
aCGTTCTTCGCTGGTTTTGGAcctcctcctaccttgTGACCCCCCTCGGCTAt
    20+[15]+16
cow, PRNP G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R Low
TN0201 tCAATGGAACAAACCCAGTAAg...gAAGCATGTGGCAGGAGCTGCTGCa
aGTTACCTTGTTTGGGTCATTc...cTTCGTACACCGTCCTCGACGACGt
    20+[16]+23
cow, PRNP G (std.) +63-Goldy, +231 WT GG(V&B) 23027955 R No
TN0205 tCCAAGCACTGTTTAAGTctggatgttggtcaaaACCCAATTTGATGATGTa
aGGTTCGTGACAAATTCAgacctacaaccagtttTGGGTTAAACTACTACAt
    17+[16]+17
cow, OFD1Y G (std.) +63-Goldy WT GG(V&B) 23027955 R No



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0147 cCAGGCCCTCATTGTCacccagACCATGAAGGGCCTGg
gGTCCGGGAGTAACAGtgggtcTGGTACTTCCCGGACc
    5F+[6]+5F
cow, BLG G ZPU0033 ZPU0034 (N/A) (N/A) Sangamo NHEJ 21912434 R
ZN0147-T002 cCAGGCCCTCATCGTCacccagACCATGAAGGGCCTGg
gGTCCGGGAGTAGCAGtgggtcTGGTACTTCCCGGACc
    5F+[6]+5F
cow, BLG, exon with SNP G ZPU0033-T002 ZPU0034 (N/A) (N/A) Sangamo NHEJ 21912434 R Low
ZN0147B gGCCCTCATTGTCACCcagaccATGAAGGGCCTGGATa
cCGGGAGTAACAGTGGgtctggTACTTCCCGGACCTAt
    5F+[6]+5F
cow, BLG G ZPU0035 ZPU0036 (N/A) (N/A) Sangamo NHEJ 21912434 R Low




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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