EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting sequences from silk worm (Bombyx mori): 3 ZFN, 2 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0206 tGGATCCACATGATCCTGTcatcagtcggttagccACACAGCTCTTTAAAAAAa
aCCTAGGTGTACTAGGACAgtagtcagccaatcggTGTGTCGAGAAATTTTTTt
    18+[16]+18
silk worm, BmBLOS2 G (std.) +63 EL+KK GG-PCR NHEJ, NHEJ-DisDel 23028749 R
TN0207 tCACCAAGTACGCAGATCTaaggagcctagccgcgAACCTGAACAAGACCCTTa
aGTGGTTCATGCGTCTAGAttcctcggatcggcgcTTGGACTTGTTCTGGGAAt
    18+[16]+18
silk worm, BmBLOS2 G (std.) +63 EL+KK GG-PCR NHEJ, NHEJ-DisDel 23028749 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0087 cGCGATCACCaagtacGCAGATCTAa
gCGCTAGTGGttcatgCGTCTAGATt
    3F+[6]+3F
silk worm, BmBLOS2 G ZP10547 ZP10546 TGQK WT MA NHEJ 20692340 R
ZN0088 gAGTCTCAGCacagaaGACATAGAAa
cTCAGAGTCGtgtcttCTGTATCTTt
    3F+[6]+3F
silk worm, BmBLOS2 G ZP10549 ZP10548 TGQK WT MA NHEJ 20692340 R
ZN0089 aGTTACTTACacgtggGCCGATGTCa
tCAATGAATGtgcaccCGGCTACAGt
    3F+[6]+3F
silk worm, Bmwh3 G ZP10551 ZP10550 TGQK WT MA NHEJ 20692340 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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