EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting sequences from thale cress (Arabidopsis thaliana): 9 ZFN, 5 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0019 tCCATCTCCTCAAGGATGTTc...cAAGAACATTGTGAAGAGTCTAGa
aGGTAGAGGAGTTCCTACAAg...gTTCTTGTAACACTTCTCAGATCt
    19+[15]+22
thale cress, TT4 G (std.) +231 WT GG(V&B) 21493687 R
TN0020 tGTCGTCTTCTGCACTacctccggcgtcgacATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAtggaggccgcagctgTACGGACCACGACTGAt
    15+[15]+16
thale cress, TT4 G (std.) +231 WT GG(V&B) 21493687 R
TN0021 tCCGGATGCTCCTCTTgacaaggtctgtattgtcAGTTGTGGTTTGTCTa
aGGCCTACGAGGAGAActgttccagacataacagTCAACACCAAACAGAt
    15+[18]+15
thale cress, ADH1 G (std.) +231 WT GG(V&B) NHEJ, HR 21493687 R
TN0038 tCCCACTTCTCAAgcgcttaagacaagaacaAGGAGGAGGAGTa
aGGGTGAAGAGTTcgcgaattctgttcttgtTCCTCCTCCTCAt
    12+[18]+12
thale cress, PDS3 G (others) Hax3 (N/A) idTALE 22271303 R
TN0039 tCCTTTTACCAGAt...aTAAATTAGTTGGa
aGGAAAATGGTCTa...tATTTAATCAACCt
    12+[36]+12
thale cress, PDS3 G (others) Hax3 (N/A) idTALE 22271303 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0077 cCGCCGCCACcgtaGGAGGAGGAg
gGCGGCGGTGgcatCCTCCTCCTc
    3F+[4]+3F
thale cress, ATABI4 G ZP10501 ZP10500 LRGS D+R MA NHEJ 20508151 R
ZN0078 tATCTTCGGCcatgaaGCTGGAGGGt
aTAGAAGCCGgtacttCGACCTCCCa
    3F+[6]+3F
thale cress, ADH1 G ZP10502 ZP10508 LRGS WT, EL+KK OPEN NHEJ 20508152 R
ZN0079 cGGCGTCGACatgcctGGTGCTGACt
gCCGCAGCTGtacggaCCACGACTGa
    3F+[6]+3F
thale cress, TT4 G ZP10511 ZP10525 LRGS WT, EL+KK OPEN NHEJ 20508152 R
ZN0094 tCTCCACAACatcagGATGACGAAg
aGAGGTGTTGtagtcCTACTGCTTc
    3F+[5]+3F
thale cress, ATMPK8 G ZP10682 ZP10590 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0095 cCTCTTCGTCctatcgGCAGAGGCGc
gGAGAAGCAGgatagcCGTCTCCGCg
    3F+[6]+3F
thale cress, ATMPK11 G ZP10568 ZP10604 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0096 aGCCAGCGACggtggtGGTGGTGGCt
tCGGTCGCTGccaccaCCACCACCGa
    3F+[6]+3F
thale cress, ATMKK9 G ZP10668 ZP10663 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0097 cTTCTTCATCcagatGTTGTTGAGa
gAAGAAGTAGgtctaCAACAACTCt
    3F+[5]+3F
thale cress, MPK15 G ZP10587 ZP10683 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0098 gCCCTTCCACaacaacGGAGAAGCTa
cGGGAAGGTGttgttgCCTCTTCGAt
    3F+[6]+3F
thale cress, MAPKKK18 G ZP10672 ZP10634 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0099 gAGCTACGCCgtagccGGAGACGCCa
cTCGATGCGGcatcggCCTCTGCGGt
    3F+[6]+3F
thale cress, GA3OX2 G ZP10652 ZP10636 (N/A) EL+KK CoDA NHEJ 21151135 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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