EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
ZFN targeting sequences from (artificial): 48 ZFN records match.
(Click on ID to see details)


ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZNA001
(Z-M)
GAGGAGGCTn
CTCCTCCGAn
    3F
(artificial) ZP00007 QNKKGLD(G4S)3 WT 8577732 R
ZNA002
(Z-M)
GAGGGATGTn
CTCCCTACAn
    3F
(artificial) ZP00008 QNKKGLD(G4S)3 WT 8577732 R
ZNA003
(Z-M)
GGGGCGGGGc
CCCCGCCCCg
    3F
(artificial) ZP00001 QNKKGLD(G4S)3 WT 8895094 R
ZNA003B
(Z-M/R)
GGGGCGGGGt
CCCCGCCCCa
    3F
(artificial) ZP00001 QNKKGLD(G4S)3 WT 9426005 R
ZNA004
(Z-M/R)
GGGGAAGAAt
CCCCUUCUUa
    3F
(artificial) ZP00003 QNKKGLD(G4S)3 WT 9426005 R
ZNA005
(Z-M)
GGGGCGGAAn
CCCCGCCTTn
    3F
(artificial) ZP00009 QNKKGLD(G4S)3 WT 9862996 R
ZNA005-T002
(Z-M)
GGGGCAGAAn
CCCCGTCTTn
    3F
(artificial) ZP00009-T002 QNKKGLD(G4S)3 WT 9862996 R
ZNA005-T003
(Z-M)
GGGTCAGAAn
CCCAGTCTTn
    3F
(artificial) ZP00009-T003 QNKKGLD(G4S)3 WT 9862996 R
ZNA005B cTTCCGCCCCn(x)GGGGCGGAAg
gAAGGCGGGGn(x)CCCCGCCTTc
    3F+[x]+3F
(artificial) ZP00009 ZP00009 QNKKGLD(G4S)3 WT 10954606 R
ZNA004B gTTCTTCCCCn(x)GGGGAAGAAt
cAAGAAGGGGn(x)CCCCTTCTTa
    3F+[x]+3F
(artificial) ZP00003 ZP00003 QNKKGLD(G4S)3 WT 10954606 R
ZNA004C nTTCTTCCCCn(4-35)GGGGAAGAAn
nAAGAAGGGGn(4-35)CCCCTTCTTn
    3F+[4-35]+3F
(artificial) ZP00003 ZP00003 QNKK + (18,6,2,0,-3,-6)aa WT 11113203 R
ZNA005C nTTCCGCCCCn(8)GGGGCGGAAn
nAAGGCGGGGn(8)CCCCGCCTTn
    3F+[8]+3F
(artificial) ZP00009 ZP00009 QNKKGLD(G4S)3 WT 11113203 R
ZNA004D nTTCTTCCCCn(8)GGGGCGGAAn
nAAGAAGGGGn(8)CCCCGCCTTn
    3F+[8]+3F
(artificial) ZP00003 ZP00009 QNKKGLD(G4S)3 WT 11113203 R
ZNA004E nTTCTTCCCCn(6)GCGTGGGCGn
nAAGAAGGGGn(6)CGCACCCGCn
    3F+[6]+3F
(artificial) ZP00003 ZP00002 QNKK WT 12730593 R
ZNA006 nACCATCTTCgaatttAGGGATAACn
nTGGTAGAAGcttaaaTCCCTATTGn
    3F+[6]+3F
(artificial) ZP00106 ZP00107 AAARA+(0/16aa) WT MA 16039907 R
ZNA006B nACCATCTTCgaatttGAAGATGGTn
nTGGTAGAAGcttaaaCTTCTACCAn
    3F+[6]+3F
(artificial) ZP00106 ZP00106 AAARA+(0/16aa) WT MA 16039907 R
ZNA006C nGTTATCCCTgaatttAGGGATAACn
nCAATAGGGActtaaaTCCCTATTGn
    3F+[6]+3F
(artificial) ZP00107 ZP00107 AAARA+(0/16aa) WT MA 16039907 R
ZNA007 nAGCAACCTCnnnnnnGCGTGGGCGn
nTCGTTGGAGnnnnnnCGCACCCGCn
    3F+[6]+3F
(artificial) ZP10015 ZP00002 (3aa)+QNKK WT MA+Natural 16169774 R
ZNA008 nTGCTCCCCCnnnnnnGCGTGGGCGn
nACGAGGGGGnnnnnnCGCACCCGCn
    3F+[6]+3F
(artificial) ZP10016, ZP10017 ZP00002 (3aa)+QNKK WT MA+Natural 16169774 R
ZNA009 nCGCCCACGCatgccaGCGTGGGCGn
nGCGGGTGCGtacggtCGCACCCGCn
    3F+[6]+3F
(artificial) ZP00002 ZP00002 (3aa)+QNKK WT MA 16262717 R
ZNA010 aCACTGCGGCtttaaaGCCGCAGTGc
tGTGACGCCGaaatttCGGCGTCACg
    3F+[6]+3F
(artificial) ZP00113; ZP00114; ZP00115 ZP00113; ZP00114; ZP00115 AAARA(5aa?) WT MA 18026168 R
ZNA011 aTGCAGCATCtttaaaGATGCTGCAc
tACGTCGTAGaaatttCTACGACGTg
    3F+[6]+3F
(artificial) ZP00116; ZP00117 ZP00116; ZP00117 AAARA(5aa?) WT MA 18026168 R
ZNA012 aGGCTTCTGCtttaaaGCAGAAGCCc
tCCGAAGACGaaatttCGTCTTCGGg
    3F+[6]+3F
(artificial) ZP00118; ZP00119; ZP00120 ZP00118; ZP00119; ZP00120 AAARA(5aa?) WT MA 18026168 R
ZNA013 nCCCGAGGGGAACCCGGGTnnnnnACCCGGGTTCCCCTCGGGn
nGGGCTCCCCTTGGGCCCAnnnnnTGGGCCCAAGGGGAGCCCn
    6F+[5]+6F
(artificial) ZP00105 ZP00105 (N/A) WT Sangamo 18334988 R
ZNA014 nTGCATCCATnnnnnnATGGATGCAn
nACGTAGGTAnnnnnnTACCTACGTn
    3F+[6]+3F
(artificial) ZP00121 ZP00121 (N/A) WT MA 18725642 R
ZNA015 nTTCCCACAAnnnnnnTTGTGGGAAn
nAAGGGTGTTnnnnnnAACACCCTTn
    3F+[6]+3F
(artificial) ZP00122 ZP00122 (N/A) WT MA 18725642 R
ZNA016 cAGCACCAACaccaGCTGCTGTCt
gTCGTGGTTGtggtCGACGACAGa
    3F+[4]+3F
(artificial) ZP00123 ZP00124 (N/A) WT MA 18725642 R
ZNA017
(Z-S2)
GGTCGGGACcatatgtatacatatgtatacGTTGCGGGAt
CCAGCCCTGgtatacatatgtatacatatgCAACGCCCTa
    3F+[21]+3F
(artificial) ZP00125 ZP00097 TGEK(G4S)3 Snase MA 18980382 R
ZNA017B
(Z-M)
GTTGCGGGAt
CAACGCCCTa
    3F
(artificial) ZP00097 TGEK(G4S)3 Snase MA 18980382 R Low
ZNA017C
(Z-M)
GGTCGGGACc
CCAGCCCTGg
    3F
(artificial) ZP00125 TGEK(G4S)3 Snase MA 18980382 R Low
ZNA017D
(Z-M)
(target sequence error)
(please contact us)

    6F
(artificial) ZP00126 TGEK(G4S)3 Snase MA 18980382 R Low
ZNA018 nATTATCCGAGTTtaatagAACTCGGATAATn
nTAATAGGCTCAAattatcTTGAGCCTATTAn
    3F+[6]+3F
(artificial) ZP00127 ZP00127 LRGS WT Sangamo 19112554 R
ZNA019 nCGAGTTCTTGTAcaccagTACAAGAACTCGn
nGCTCAAGAACATgtggtcATGTTCTTGAGCn
    3F+[6]+3F
(artificial) ZP00128 ZP00128 LRGS WT Sangamo 19112554 R
ZNA020
(Z-S1)
GAAAACGGTCGGGACCGAa
CTTTTGCCAGCCCTGGCTt
    6F
(artificial) ZP00108 TGEK(G4S)3 WT MA 19428709 R
ZNA017E
(Z-S2)
nGGTCGGGACcn(x)GTTGCGGGAt
nCCAGCCCTGgn(x)CAACGCCCTa
    3F+[x]+3F
(artificial) ZP00125 ZP00097 TGEK(G4S)3 WT MA 19825368 R
ZNA014-T002 cAGCATCCATgtaagtATGGATGCTg
gTCGTAGGTAcattcaTACCTACGAc
    3F+[6]+3F
(artificial) ZP00121-T002 ZP00121-T002 (N/A) WT MA 21029755 R Low
ZNA014-T003 cGGCATCCATgtaagtATGGATGCCg
gCCGTAGGTAcattcaTACCTACGGc
    3F+[6]+3F
(artificial) ZP00121-T003 ZP00121-T003 (N/A) WT MA 21029755 R
ZNA014-T004 cTGCATCCCTgtaagtAGGGATGCAg
gACGTAGGGAcattcaTCCCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T004 ZP00121-T004 (N/A) WT MA 21029755 R Low
ZNA014-T005 cTGCATCCGTgtaagtACGGATGCAg
gACGTAGGCAcattcaTGCCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T005 ZP00121-T005 (N/A) WT MA 21029755 R
ZNA014-T006 cTGCATCCTTgtaagtAAGGATGCAg
gACGTAGGAAcattcaTTCCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T006 ZP00121-T006 (N/A) WT MA 21029755 R
ZNA014-T007 cTGCATCCACgtaagtGTGGATGCAg
gACGTAGGTGcattcaCACCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T007 ZP00121-T007 (N/A) WT MA 21029755 R
ZNA014-T008 cAGCATCCTCgtaagtGAGGATGCTg
gTCGTAGGAGcattcaCTCCTACGAc
    3F+[6]+3F
(artificial) ZP00121-T008 ZP00121-T008 (N/A) WT MA 21029755 R
ZNA014-T009 cTGCATCCATgtaagtATGGCTGCAg
gACGTAGGTAcattcaTACCGACGTc
    3F+[6]+3F
(artificial) ZP00121 ZP00121-T009 (N/A) WT MA 21029755 R
ZNA014-T010 cTGCATCCATgtaagtATGGACGCAg
gACGTAGGTAcattcaTACCTGCGTc
    3F+[6]+3F
(artificial) ZP00121 ZP00121-T010 (N/A) WT MA 21029755 R
ZNA006D nACCATCTTCcttaaaAGGGATAACn
nTGGTAGAAGgaatttTCCCTATTGn
    3F+[6]+3F
(artificial) ZP00106; ZP00129 ZP00107; ZP00130 AAARA(5aa?) D+R MA 21528840 R
ZNA006E nACCATCTTCACCATCTTCcttaaaAGGGATAACAGGGATAACn
nTGGTAGAAGTGGTAGAAGgaatttTCCCTATTGTCCCTATTGn
    6F+[6]+6F
(artificial) ZP00106; ZP00129; ZP00131; ZP00133 ZP00107; ZP00130; ZP00132; ZP00134 AAARA(5aa?) D+R MA 21528840 R
ZNA006F nACCATCTTCACCATCTTCn(x)AGGGATAACAGGGATAACn
nTGGTAGAAGTGGTAGAAGn(x)TCCCTATTGTCCCTATTGn
    6F+[x]+6F
(artificial) ZP00133 ZP00134 AAARA(5aa?) D+R MA 21528840 R
ZNAU01 (N/A)
    
(artificial) (N/A) (N/A) (N/A) WT MA 22082504 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
GSearch EENdb with this gene symbol
RSearch EENdb with this reference

©2012-2013  PKU Zebrafish Functional Genomics Group
School of Life Sciences, Peking University, Beijing, China
Reference of EENdb    Contact us