EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting sequences from (artificial): 48 ZFN, 98 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TNA001 tATCAAGATTCTCn(16)GAGAATCTTGATa
aTAGTTCTAAGAGn(16)CTCTTAGAACTAt
    12+[16]+12
(artificial) (std.) +235 WT (N/A) 20660643 R
TNA002 tCCCAGAAGTAAAn(16)TTTACTTCTGGGa
aGGGTCTTCATTTn(16)AAATGAAGACCCt
    12+[16]+12
(artificial) (std.) +235 WT (N/A) 20660643 R No
TNA003 tCCGGATGCTCCTn(18)AGGAGCATCCGGa
aGGCCTACGAGGAn(18)TCCTCGTAGGCCt
    12+[18]+12
(artificial) (std.) +235 WT (N/A) 20660643 R Low
TNA004 tAGACAAACCACAn(18)TGTGGTTTGTCTa
aTCTGTTTGGTGTn(18)ACACCAAACAGAt
    12+[18]+12
(artificial) (std.) +235 WT (N/A) 20660643 R No
TNA005 tACCCCTCTCCGCTn(18)AGCGGAGAGGGGTa
aTGGGGAGAGGCGAn(18)TCGCCTCTCCCCAt
    13+[18]+13
(artificial) (std.) +235 WT (N/A) 20660643 R
TNA006 (N/A)
    18+[15-30]+18
(artificial) +235 WT (N/A) 20660643 R
TNA007 (N/A)
    24+[13-30]+24
(artificial) +235 WT (N/A) 20660643 R
TNA008
(T-M)
tATAAACCCCCTCCAACCAGGTGCTAA
aTATTTGGGGGAGGTTGGTCCACGATT
    26
(artificial) (N*; NS-N; others) +297 WT (N/A) 20699274 R
TNA007B
(T-M)
tGCATCTCCCCCTACTGTACACCAC
aCGTAGAGGGGGATGACATGTGGTG
    24
(artificial) (NS-N; others) +297 WT (N/A) 20699274 R
TNA008B
(T-M-N)
tATAAACCCCCTCCAACCAGGTGCTAA
aTATTTGGGGGAGGTTGGTCCACGATT
    26
(artificial) (N*; NS-N; others) 4+288(N) WT (N/A) 20699274 R
TNA008C
(T-D-N)
TTAGCACCTGGTTGGAGGGGGTTTATa...tATAAACCCCCTCCAACCAGGTGCTAA
AATCGTGGACCAACCTCCCCCAAATAt...aTATTTGGGGGAGGTTGGTCCACGATT
    26+[x+2]+26
(artificial) (N*; NS-N; others) 4+288(N) WT (N/A) 20699274 R
TNA008D tATAAACCCCCTCCAACCAGGTGCTAAn(x)TTAGCACCTGGTTGGAGGGGGTTTATa
aTATTTGGGGGAGGTTGGTCCACGATTn(x)AATCGTGGACCAACCTCCCCCAAATAt
    26+[x]+26
(artificial) (N*; NS-N; others) +297 WT (N/A) 20699274 R
TNA008E/TNA007C tATAAACCCCCTCCAACCAGGTGCTAAn(x)GTGGTGTACAGTAGGGGGAGATGCa
aTATTTGGGGGAGGTTGGTCCACGATTn(x)CACCACATGTCATCCCCCTCTACGt
    26+[x]+24
(artificial) (N*; NS-N; others) +297, +297 WT (N/A) 20699274 R
TNA009 tATAAATACCTTCTn(2-24)AGAAGGTATTTATa
aTATTTATGGAAGAn(2-24)TCTTCCATAAATAt
    13+[2-24]+13
(artificial) (others) +28, +39, +50, +63, +79, +95 WT (N/A) 21179091 R
TNA010 tCCCTTTATCTCTcgaatcccgggtacgaAGAGATAAAGGGa
aGGGAAATAGAGAgcttagggcccatgctTCTCTATTTCCCt
    12+[16]+12
(artificial) (NS-N) +286 WT (unnamed) 21262818 R
TNA008F tGCATCTCCCCCTACTGTACACCACc...cTTAGCACCTGGTTGGAGGGGGTTTATa
aCGTAGAGGGGGATGACATGTGGTGg...gAATCGTGGACCAACCTCCCCCAAATAt
    24+[19]+26
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA011 tATAAGGAACGTGCTGCTactcatcctagtcctgtTGCTGCCAAGCTATTTAATa
aTATTCCTTGCACGACGAtgagtaggatcaggacaACGACGGTTCGATAAATTAt
    17+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA012 tATTAAATAGCTTGGCAGCAa...tTGCTGCCAAGCTATTTAATa
aTAATTTATCGAACCGTCGTt...aACGACGGTTCGATAAATTAt
    19+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA013 tATGACACCCGGTGTGGGactcatcctagtcctgtTGCTGCCAAGCTATTTAATa
aTACTGTGGGCCACACCCtgagtaggatcaggacaACGACGGTTCGATAAATTAt
    17+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA014 tACTGTTGACCCAATGCGactcatcctagtcctgtTGCTGCCAAGCTATTTAATa
aTGACAACTGGGTTACGCtgagtaggatcaggacaACGACGGTTCGATAAATTAt
    17+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA015 tGCATATCCCCCAGCCAGACAAACa...tTGCTGCCAAGCTATTTAATa
aCGTATAGGGGGTCGGTCTGTTTGt...aACGACGGTTCGATAAATTAt
    23+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA016 tAATAACAATCAACCCACGTGGTTa...tTGCTGCCAAGCTATTTAATa
aTTATTGTTAGTTGGGTGCACCAAt...aACGACGGTTCGATAAATTAt
    23+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA017 tTCCAACCCAACCCTATCTTTCAa...tTGCTGCCAAGCTATTTAATa
aAGGTTGGGTTGGGATAGAAAGTt...aACGACGGTTCGATAAATTAt
    22+[17]+19
(artificial) (NS-N; others) +297 WT GG(Y) 21459844 R
TNA018 tGTCTACCAAGAACACCCTTAGGAa...tTGCTGCCAAGCTATTTAATa
aCAGATGGTTCTTGTGGGAATCCTt...aACGACGGTTCGATAAATTAt
    23+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA019 tTCCGCAAGCAAGCGTGGAATTAAAa...tTGCTGCCAAGCTATTTAATa
aAGGCGTTCGTTCGCACCTTAATTTt...aACGACGGTTCGATAAATTAt
    24+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA020 tACCTGGCAAGTGAGCAGCCCCACa...tTGCTGCCAAGCTATTTAATa
aTGGACCGTTCACTCGTCGGGGTGt...aACGACGGTTCGATAAATTAt
    23+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA002B tCCCAGAAGTAAAn(x)TTTACTTCTGGGa
aGGGTCTTCATTTn(x)AAATGAAGACCCt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA004B tAGACAAACCACAn(x)TGTGGTTTGTCTa
aTCTGTTTGGTGTn(x)ACACCAAACAGAt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA021 tGGCACTGCTAACCCTn(x)AGGGTTAGCAGTGCCa
aCCGTGACGATTGGGAn(x)TCCCAATCGTCACGGt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA022 tCGGTTTGCTATCGTTn(x)AACGATAGCAAACCGa
aGCCAAACGATAGCAAn(x)TTGCTATCGTTTGGCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA023 tCCCAGAAGTAAACATn(x)ATGTTTACTTCTGGGa
aGGGTCTTCATTTGTAn(x)TACAAATGAAGACCCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA003B tCCGGATGCTCCTn(x)AGGAGCATCCGGa
aGGCCTACGAGGAn(x)TCCTCGTAGGCCt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA024 tACCCCTCTCCn(x)GGAGAGGGGTa
aTGGGGAGAGGn(x)CCTCTCCCCAt
    10+[x]+10
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA025 tCTTGTCCGCATGATTn(x)AATCATGCGGACAAGa
aGAACAGGCGTACTAAn(x)TTAGTACGCCTGTTCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA026 tATTCCCCACGAGCTCTn(x)AGAGCTCGTGGGGAATa
aTAAGGGGTGCTCGAGAn(x)TCTCGAGCACCCCTTAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA027 tAGACAGGAAGTGGAGCn(x)GCTCCACTTCCTGTCTa
aTCTGTCCTTCACCTCGn(x)CGAGGTGAAGGACAGAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA001B tATCAAGATTCTCn(x)GAGAATCTTGATa
aTAGTTCTAAGAGn(x)CTCTTAGAACTAt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA028 tAGACAAACCACAACTn(x)AGTTGTGGTTTGTCTa
aTCTGTTTGGTGTTGAn(x)TCAACACCAAACAGAt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA005B tACCCCTCTCCGCTn(x)AGCGGAGAGGGGTa
aTGGGGAGAGGCGAn(x)TCGCCTCTCCCCAt
    13+[x]+13
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA029 tCCGGATGCTCCTCTTn(x)AAGAGGAGCATCCGGa
aGGCCTACGAGGAGAAn(x)TTCTCCTCGTAGGCCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA030 tACCCCTCTCCGCTTCTn(x)AGAAGCGGAGAGGGGTa
aTGGGGAGAGGCGAAGAn(x)TCTTCGCCTCTCCCCAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA031 tATAATTAATAATCCACTTn(x)AAGTGGATTATTAATTATa
aTATTAATTATTAGGTGAAn(x)TTCACCTAATAATTAATAt
    18+[x]+18
(artificial) (NS-N) AvrBs4-BB, AvrBs4-NH, AvrBs4-EH, AvrBs4-EC, AvrBs4-NC KV+EA (N/A) 21813459 R
TNA031B tATAATTAATAATCCACTTaggctcgagttacAGAGGGTTAGGTTTATAGa
aTATTAATTATTAGGTGAAtccgagctcaatgTCTCCCAATCCAAATATCt
    18+[13]+18
(artificial) (NS-N) +17 KV+EA (N/A) 21813459 R
TNA032 tCCCACTTCTCAAn(x)TTGAGAAGTGGGa
aGGGTGAAGAGTTn(x)AACTCTTCACCCt
    12+[x]+12
(artificial) (NS-N; others) Hax3 (N/A) idTALE 22271303 R
TNA033 tATATAAGCACGTATCTn(x)AGATACGTGCTTATATa
aTATATTCGTGCATAGAn(x)TCTATGCACGAATATAt
    16+[x]+16
(artificial) (N*; NS-N; others) N0/1/2/3/4-C0, N3-C1/2/3/4/5 WT (N/A) 22301904 R
TNA033B nATATAAGCACGTATCTn(16)AGATACGTGCTTATATn
nTATATTCGTGCATAGAn(16)TCTATGCACGAATATAn
    16+[16]+16
(artificial) (N*; NS-N; others) N2/3/4-C0, N3-C1/2 WT (N/A) 22301904 R
TNA034 tGTCGTCTTCTGCACTn(x)AGTGCAGAAGACGACa
aCAGCAGAAGACGTGAn(x)TCACGTCTTCTGCTGt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA034B tGTCGTCTTCTGCACTn(x)AGTGCAGAAGACGACa
aCAGCAGAAGACGTGAn(x)TCACGTCTTCTGCTGt
    15+[x]+15
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA034C tGTCGTCTTCTGCACTn(x)AGTGCAGAAGACGACa
aCAGCAGAAGACGTGAn(x)TCACGTCTTCTGCTGt
    15+[x]+15
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA034D tGTCGTCTTCTGCACTn(x)AGTGCAGAAGACGACa
aCAGCAGAAGACGTGAn(x)TCACGTCTTCTGCTGt
    15+[x]+15
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA035 tAGTCAGCACCAGGCATn(x)ATGCCTGGTGCTGACTa
aTCAGTCGTGGTCCGTAn(x)TACGGACCACGACTGAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA035B tAGTCAGCACCAGGCATn(x)ATGCCTGGTGCTGACTa
aTCAGTCGTGGTCCGTAn(x)TACGGACCACGACTGAt
    16+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA035C tAGTCAGCACCAGGCATn(x)ATGCCTGGTGCTGACTa
aTCAGTCGTGGTCCGTAn(x)TACGGACCACGACTGAt
    16+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R Low
TNA035D tAGTCAGCACCAGGCATn(x)ATGCCTGGTGCTGACTa
aTCAGTCGTGGTCCGTAn(x)TACGGACCACGACTGAt
    16+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA036 tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA036-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R No
TNA036B tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R Low
TNA036B-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R
TNA036C tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA036C-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R
TNA036D tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA036D-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R
TNA036E tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA036E-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R
TNA036F tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA036F-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R Low
TNA037 tCCATTCCTATGACTGTn(x)AGCTATTGTGTGAGTATa
aGGTAAGGATACTGACAn(x)TCGATAACACACTCATAt
    16+[x]+17
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA037B tCCATTCCTATGACTGTn(x)AGCTATTGTGTGAGTATa
aGGTAAGGATACTGACAn(x)TCGATAACACACTCATAt
    16+[x]+17
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA038 tCCCTCGCTTTCTGATn(x)ATCGGGCGCTGTCGGGa
aGGGAGCGAAAGACTAn(x)TAGCCCGCGACAGCCCt
    15+[x]+16
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA038B tCCCTCGCTTTCTGATn(x)ATCGGGCGCTGTCGGGa
aGGGAGCGAAAGACTAn(x)TAGCCCGCGACAGCCCt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA039 tCCTTTTAAGAAGTTGGCATn(x)ATAATACAATTTAAAACCTGGGTCTCTa
aGGAAAATTCTTCAACCGTAn(x)TATTATGTTAAATTTTGGACCCAGAGAt
    19+[x]+27
(artificial) (std.) +231 WT GG(V&B) 23028976 R Low
TNA039B tCCTTTTAAGAAGTTGGCATn(x)ATAATACAATTTAAAACCTGGGTCTCTa
aGGAAAATTCTTCAACCGTAn(x)TATTATGTTAAATTTTGGACCCAGAGAt
    19+[x]+27
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA040 tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA040-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R No
TNA040B tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA040B-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R No
TNA040C tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA040C-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R
TNA040D tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA040D-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R No
TNA040E tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA040E-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R No
TNA041 tGCTGACGACTTTGTCCCTn(x)ACTCGGTGGGCTTGTTGGCGGa
aCGACTGCTGAAACAGGGAn(x)TGAGCCACCCGAACAACCGCCt
    18+[x]+21
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA041B tGCTGACGACTTTGTCCCTn(x)ACTCGGTGGGCTTGTTGGCGGa
aCGACTGCTGAAACAGGGAn(x)TGAGCCACCCGAACAACCGCCt
    18+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA042 tCTTTACAAGGAGCACGTn(x)ATTAAAGGAGTTTAGCGTGACa
aGAAATGTTCCTCGTGCAn(x)TAATTTCCTCAAATCGCACTGt
    17+[x]+21
(artificial) (std.) +231 WT GG(V&B) 23028976 R Low
TNA042B tCTTTACAAGGAGCACGTn(x)ATTAAAGGAGTTTAGCGTGACa
aGAAATGTTCCTCGTGCAn(x)TAATTTCCTCAAATCGCACTGt
    17+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA043 tCTACCTTCATTGATGTCCCTn(x)ATGAAAGGCAAAGCTCCTCTa
aGATGGAAGTAACTACAGGGAn(x)TACTTTCCGTTTCGAGGAGAt
    20+[x]+20
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA043B tCTACCTTCATTGATGTCCCTn(x)ATGAAAGGCAAAGCTCCTCTa
aGATGGAAGTAACTACAGGGAn(x)TACTTTCCGTTTCGAGGAGAt
    20+[x]+20
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA044 tAGCTCAGTCATCAAAGGCAAAGCTn(x)ACTGGCTCTGGAGGTTACa
aTCGAGTCAGTAGTTTCCGTTTCGAn(x)TGACCGAGACCTCCAATGt
    24+[x]+18
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA044B tAGCTCAGTCATCAAAGGCAAAGCTn(x)ACTGGCTCTGGAGGTTACa
aTCGAGTCAGTAGTTTCCGTTTCGAn(x)TGACCGAGACCTCCAATGt
    24+[x]+18
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA045 tATCCAAAAGAGCGGTCTGATn(x)ATCCTCAGGATTGTTGCa
aTAGGTTTTCTCGCCAGACTAn(x)TAGGAGTCCTAACAACGt
    20+[x]+17
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA045B tATCCAAAAGAGCGGTCTGATn(x)ATCCTCAGGATTGTTGCa
aTAGGTTTTCTCGCCAGACTAn(x)TAGGAGTCCTAACAACGt
    20+[x]+17
(artificial) (NK) +231 WT GG(V&B) 23028976 R Low
TNA046 tGGCGAAAGAGTCCACCACCATn(x)AAGTTCAAGCCATGTCGTa
aCCGCTTTCTCAGGTGGTGGTAn(x)TTCAAGTTCGGTACAGCAt
    21+[x]+18
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA046B tGGCGAAAGAGTCCACCACCATn(x)AAGTTCAAGCCATGTCGTa
aCCGCTTTCTCAGGTGGTGGTAn(x)TTCAAGTTCGGTACAGCAt
    21+[x]+18
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA047 tGCTTGCTTACCTGACCTATn(x)ACATATTTTTGTTTTCAGa
aCGAACGAATGGACTGGATAn(x)TGTATAAAAACAAAAGTCt
    19+[x]+18
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA047B tGCTTGCTTACCTGACCTATn(x)ACATATTTTTGTTTTCAGa
aCGAACGAATGGACTGGATAn(x)TGTATAAAAACAAAAGTCt
    19+[x]+18
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA048 tCGGCCTGCCGGCCCTGGCCGGTn(x)AGGTGGCGGCCGGGCCCTGAGGCGCGGGa
aGCCGGACGGCCGGGACCGGCCAn(x)TCCACCGCCGGCCCGGGACTCCGCGCCCt
    22+[x]+28
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA048B tCGGCCTGCCGGCCCTGGCCGGTn(x)AGGTGGCGGCCGGGCCCTGAGGCGCGGGa
aGCCGGACGGCCGGGACCGGCCAn(x)TCCACCGCCGGCCCGGGACTCCGCGCCCt
    22+[x]+28
(artificial) (NK) +231 WT GG(V&B) 23028976 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZNA001
(Z-M)
GAGGAGGCTn
CTCCTCCGAn
    3F
(artificial) ZP00007 QNKKGLD(G4S)3 WT 8577732 R
ZNA002
(Z-M)
GAGGGATGTn
CTCCCTACAn
    3F
(artificial) ZP00008 QNKKGLD(G4S)3 WT 8577732 R
ZNA003
(Z-M)
GGGGCGGGGc
CCCCGCCCCg
    3F
(artificial) ZP00001 QNKKGLD(G4S)3 WT 8895094 R
ZNA003B
(Z-M/R)
GGGGCGGGGt
CCCCGCCCCa
    3F
(artificial) ZP00001 QNKKGLD(G4S)3 WT 9426005 R
ZNA004
(Z-M/R)
GGGGAAGAAt
CCCCUUCUUa
    3F
(artificial) ZP00003 QNKKGLD(G4S)3 WT 9426005 R
ZNA005
(Z-M)
GGGGCGGAAn
CCCCGCCTTn
    3F
(artificial) ZP00009 QNKKGLD(G4S)3 WT 9862996 R
ZNA005-T002
(Z-M)
GGGGCAGAAn
CCCCGTCTTn
    3F
(artificial) ZP00009-T002 QNKKGLD(G4S)3 WT 9862996 R
ZNA005-T003
(Z-M)
GGGTCAGAAn
CCCAGTCTTn
    3F
(artificial) ZP00009-T003 QNKKGLD(G4S)3 WT 9862996 R
ZNA005B cTTCCGCCCCn(x)GGGGCGGAAg
gAAGGCGGGGn(x)CCCCGCCTTc
    3F+[x]+3F
(artificial) ZP00009 ZP00009 QNKKGLD(G4S)3 WT 10954606 R
ZNA004B gTTCTTCCCCn(x)GGGGAAGAAt
cAAGAAGGGGn(x)CCCCTTCTTa
    3F+[x]+3F
(artificial) ZP00003 ZP00003 QNKKGLD(G4S)3 WT 10954606 R
ZNA004C nTTCTTCCCCn(4-35)GGGGAAGAAn
nAAGAAGGGGn(4-35)CCCCTTCTTn
    3F+[4-35]+3F
(artificial) ZP00003 ZP00003 QNKK + (18,6,2,0,-3,-6)aa WT 11113203 R
ZNA005C nTTCCGCCCCn(8)GGGGCGGAAn
nAAGGCGGGGn(8)CCCCGCCTTn
    3F+[8]+3F
(artificial) ZP00009 ZP00009 QNKKGLD(G4S)3 WT 11113203 R
ZNA004D nTTCTTCCCCn(8)GGGGCGGAAn
nAAGAAGGGGn(8)CCCCGCCTTn
    3F+[8]+3F
(artificial) ZP00003 ZP00009 QNKKGLD(G4S)3 WT 11113203 R
ZNA004E nTTCTTCCCCn(6)GCGTGGGCGn
nAAGAAGGGGn(6)CGCACCCGCn
    3F+[6]+3F
(artificial) ZP00003 ZP00002 QNKK WT 12730593 R
ZNA006 nACCATCTTCgaatttAGGGATAACn
nTGGTAGAAGcttaaaTCCCTATTGn
    3F+[6]+3F
(artificial) ZP00106 ZP00107 AAARA+(0/16aa) WT MA 16039907 R
ZNA006B nACCATCTTCgaatttGAAGATGGTn
nTGGTAGAAGcttaaaCTTCTACCAn
    3F+[6]+3F
(artificial) ZP00106 ZP00106 AAARA+(0/16aa) WT MA 16039907 R
ZNA006C nGTTATCCCTgaatttAGGGATAACn
nCAATAGGGActtaaaTCCCTATTGn
    3F+[6]+3F
(artificial) ZP00107 ZP00107 AAARA+(0/16aa) WT MA 16039907 R
ZNA007 nAGCAACCTCnnnnnnGCGTGGGCGn
nTCGTTGGAGnnnnnnCGCACCCGCn
    3F+[6]+3F
(artificial) ZP10015 ZP00002 (3aa)+QNKK WT MA+Natural 16169774 R
ZNA008 nTGCTCCCCCnnnnnnGCGTGGGCGn
nACGAGGGGGnnnnnnCGCACCCGCn
    3F+[6]+3F
(artificial) ZP10016, ZP10017 ZP00002 (3aa)+QNKK WT MA+Natural 16169774 R
ZNA009 nCGCCCACGCatgccaGCGTGGGCGn
nGCGGGTGCGtacggtCGCACCCGCn
    3F+[6]+3F
(artificial) ZP00002 ZP00002 (3aa)+QNKK WT MA 16262717 R
ZNA010 aCACTGCGGCtttaaaGCCGCAGTGc
tGTGACGCCGaaatttCGGCGTCACg
    3F+[6]+3F
(artificial) ZP00113; ZP00114; ZP00115 ZP00113; ZP00114; ZP00115 AAARA(5aa?) WT MA 18026168 R
ZNA011 aTGCAGCATCtttaaaGATGCTGCAc
tACGTCGTAGaaatttCTACGACGTg
    3F+[6]+3F
(artificial) ZP00116; ZP00117 ZP00116; ZP00117 AAARA(5aa?) WT MA 18026168 R
ZNA012 aGGCTTCTGCtttaaaGCAGAAGCCc
tCCGAAGACGaaatttCGTCTTCGGg
    3F+[6]+3F
(artificial) ZP00118; ZP00119; ZP00120 ZP00118; ZP00119; ZP00120 AAARA(5aa?) WT MA 18026168 R
ZNA013 nCCCGAGGGGAACCCGGGTnnnnnACCCGGGTTCCCCTCGGGn
nGGGCTCCCCTTGGGCCCAnnnnnTGGGCCCAAGGGGAGCCCn
    6F+[5]+6F
(artificial) ZP00105 ZP00105 (N/A) WT Sangamo 18334988 R
ZNA014 nTGCATCCATnnnnnnATGGATGCAn
nACGTAGGTAnnnnnnTACCTACGTn
    3F+[6]+3F
(artificial) ZP00121 ZP00121 (N/A) WT MA 18725642 R
ZNA015 nTTCCCACAAnnnnnnTTGTGGGAAn
nAAGGGTGTTnnnnnnAACACCCTTn
    3F+[6]+3F
(artificial) ZP00122 ZP00122 (N/A) WT MA 18725642 R
ZNA016 cAGCACCAACaccaGCTGCTGTCt
gTCGTGGTTGtggtCGACGACAGa
    3F+[4]+3F
(artificial) ZP00123 ZP00124 (N/A) WT MA 18725642 R
ZNA017
(Z-S2)
GGTCGGGACcatatgtatacatatgtatacGTTGCGGGAt
CCAGCCCTGgtatacatatgtatacatatgCAACGCCCTa
    3F+[21]+3F
(artificial) ZP00125 ZP00097 TGEK(G4S)3 Snase MA 18980382 R
ZNA017B
(Z-M)
GTTGCGGGAt
CAACGCCCTa
    3F
(artificial) ZP00097 TGEK(G4S)3 Snase MA 18980382 R Low
ZNA017C
(Z-M)
GGTCGGGACc
CCAGCCCTGg
    3F
(artificial) ZP00125 TGEK(G4S)3 Snase MA 18980382 R Low
ZNA017D
(Z-M)
(target sequence error)
(please contact us)

    6F
(artificial) ZP00126 TGEK(G4S)3 Snase MA 18980382 R Low
ZNA018 nATTATCCGAGTTtaatagAACTCGGATAATn
nTAATAGGCTCAAattatcTTGAGCCTATTAn
    3F+[6]+3F
(artificial) ZP00127 ZP00127 LRGS WT Sangamo 19112554 R
ZNA019 nCGAGTTCTTGTAcaccagTACAAGAACTCGn
nGCTCAAGAACATgtggtcATGTTCTTGAGCn
    3F+[6]+3F
(artificial) ZP00128 ZP00128 LRGS WT Sangamo 19112554 R
ZNA020
(Z-S1)
GAAAACGGTCGGGACCGAa
CTTTTGCCAGCCCTGGCTt
    6F
(artificial) ZP00108 TGEK(G4S)3 WT MA 19428709 R
ZNA017E
(Z-S2)
nGGTCGGGACcn(x)GTTGCGGGAt
nCCAGCCCTGgn(x)CAACGCCCTa
    3F+[x]+3F
(artificial) ZP00125 ZP00097 TGEK(G4S)3 WT MA 19825368 R
ZNA014-T002 cAGCATCCATgtaagtATGGATGCTg
gTCGTAGGTAcattcaTACCTACGAc
    3F+[6]+3F
(artificial) ZP00121-T002 ZP00121-T002 (N/A) WT MA 21029755 R Low
ZNA014-T003 cGGCATCCATgtaagtATGGATGCCg
gCCGTAGGTAcattcaTACCTACGGc
    3F+[6]+3F
(artificial) ZP00121-T003 ZP00121-T003 (N/A) WT MA 21029755 R
ZNA014-T004 cTGCATCCCTgtaagtAGGGATGCAg
gACGTAGGGAcattcaTCCCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T004 ZP00121-T004 (N/A) WT MA 21029755 R Low
ZNA014-T005 cTGCATCCGTgtaagtACGGATGCAg
gACGTAGGCAcattcaTGCCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T005 ZP00121-T005 (N/A) WT MA 21029755 R
ZNA014-T006 cTGCATCCTTgtaagtAAGGATGCAg
gACGTAGGAAcattcaTTCCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T006 ZP00121-T006 (N/A) WT MA 21029755 R
ZNA014-T007 cTGCATCCACgtaagtGTGGATGCAg
gACGTAGGTGcattcaCACCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T007 ZP00121-T007 (N/A) WT MA 21029755 R
ZNA014-T008 cAGCATCCTCgtaagtGAGGATGCTg
gTCGTAGGAGcattcaCTCCTACGAc
    3F+[6]+3F
(artificial) ZP00121-T008 ZP00121-T008 (N/A) WT MA 21029755 R
ZNA014-T009 cTGCATCCATgtaagtATGGCTGCAg
gACGTAGGTAcattcaTACCGACGTc
    3F+[6]+3F
(artificial) ZP00121 ZP00121-T009 (N/A) WT MA 21029755 R
ZNA014-T010 cTGCATCCATgtaagtATGGACGCAg
gACGTAGGTAcattcaTACCTGCGTc
    3F+[6]+3F
(artificial) ZP00121 ZP00121-T010 (N/A) WT MA 21029755 R
ZNA006D nACCATCTTCcttaaaAGGGATAACn
nTGGTAGAAGgaatttTCCCTATTGn
    3F+[6]+3F
(artificial) ZP00106; ZP00129 ZP00107; ZP00130 AAARA(5aa?) D+R MA 21528840 R
ZNA006E nACCATCTTCACCATCTTCcttaaaAGGGATAACAGGGATAACn
nTGGTAGAAGTGGTAGAAGgaatttTCCCTATTGTCCCTATTGn
    6F+[6]+6F
(artificial) ZP00106; ZP00129; ZP00131; ZP00133 ZP00107; ZP00130; ZP00132; ZP00134 AAARA(5aa?) D+R MA 21528840 R
ZNA006F nACCATCTTCACCATCTTCn(x)AGGGATAACAGGGATAACn
nTGGTAGAAGTGGTAGAAGn(x)TCCCTATTGTCCCTATTGn
    6F+[x]+6F
(artificial) ZP00133 ZP00134 AAARA(5aa?) D+R MA 21528840 R
ZNAU01 (N/A)
    
(artificial) (N/A) (N/A) (N/A) WT MA 22082504 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
GSearch EENdb with this gene symbol
RSearch EENdb with this reference

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School of Life Sciences, Peking University, Beijing, China
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