EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
ZFN targeting sequences from (GFP): 12 ZFN records match.
(Click on ID to see details)


ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0002 cCGCTACCCCgaccacatGAAGCAGCAc
gGCGATGGGGctggtgtaCTTCGTCGTg
    3F+[8]+3F
eGFP G ZP10003 ZP10004 (N/A) WT MA HR 15806097 R
ZN0008 cACCATCTTCttcaagGACGACGGCa
gTGGTAGAAGaagttcCTGCTGCCGt
    3F+[6]+3F
eGFP G ZP10018 ZP10019 (3aa) WT MA HR 16169774 R
ZN0008B cACCATCTTCttcaagGACGACGGCa
gTGGTAGAAGaagttcCTGCTGCCGt
    3F+[6]+3F
eGFP G ZP10028 ZP10019 (N/A) P509T+N586S, WT, D+R OPEN 18334988 R
ZN0008C cACCATCTTCttcaagGACGACGGCa
gTGGTAGAAGaagttcCTGCTGCCGt
    3F+[6]+3F
eGFP G ZP10031, ZP10032 ZP10034 (N/A) P509T+N586S, WT, D+R OPEN 18334988 R
ZN0002B cTACCCCGACcacatGAAGCAGCAc
gATGGGGCTGgtgtaCTTCGTCGTg
    3F+[5]+3F
eGFP G ZP10066, ZP10070 ZP10073, ZP10077 LRGS WT OPEN NHEJ 18657511 R
ZN0008D cACCATCTTCttcaagGACGACGGCa
gTGGTAGAAGaagttcCTGCTGCCGt
    3F+[6]+3F
eGFP G ZP10089, ZP10092 ZP10096, ZP10099 LRGS WT OPEN NHEJ 18657511 R
ZN0020 gGGCATCGACttcaagGAGGACGGCa
cCCGTAGCTGaagttcCTCCTGCCGt
    3F+[6]+3F
eGFP G ZP10104, ZP10105 ZP10111, ZP10112 LRGS WT OPEN NHEJ 18657511 R
ZN0021 gATCCGCCACaacatcGAGGACGGCa
cTAGGCGGTGttgtagCTCCTGCCGt
    3F+[6]+3F
eGFP G ZP10120, ZP10121 ZP10128, ZP10134 LRGS WT OPEN NHEJ 18657511 R
ZN0052 cAACTACAACAGCCACAACgtctatATCATGGCCGACAAGc
gTTGATGTTGTCGGTGTTGcagataTAGTACCGGCTGTTCg
    6F+[6]+5F
eGFP G ZP10458 ZP10459 LRGS WT Sangamo NHEJ 19628861 R
ZN0132 aGTTGTCCCAATTCTTGTTgaattaGATGGTGATgtTAATGGg
tCAACAGGGTTAAGAACAActtaatCTACCACTAcaATTACCc
    6F+[6]+(3F+2+2F)
eGFP G ZP10740 ZP10741, ZP10742 (N/A) WT Sangamo NHEJ 21700836 R
ZN0020B gGGCATCGACttcaagGAGGACGGCa
cCCGTAGCTGaagttcCTCCTGCCGt
    3F+[6]+3F
eGFP G ZPU0053 ZPU0054 TGGS Sharkey-AS+R PRS NHEJ 22640377 R
ZN0020C gGGCATCGACttcaagGAGGACGGCa
cCCGTAGCTGaagttcCTCCTGCCGt
    3F+[6]+3F
eGFP G ZP10885 ZP10884 TGGS Sharkey-AS+R PRS NHEJ 22642582 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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