EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
TALEN targeting sequences from (GFP): 68 TALEN records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0015 tCCATGGCCAACACTTGTCACTACTTTc...aAGATACCCAGATCATATGAAGCGGCa
aGGTACCGGTTGTGAACAGTGATGAAAg...tTCTATGGGTCTAGTATACTTCGCCGt
    26+[25]+25
GFP G (std.) +231 WT GG(V&B) 21493687 R
TN0016 tACAACTACAACAGCCACAACGTCTa...aAGAACGGCATCAAGGTGAACTTCAAGa
aTGTTGATGTTGTCGGTGTTGCAGAt...tTCTTGCCGTAGTTCCACTTGAAGTTCt
    24+[21]+27
eGFP G (std.) +231 WT GG(V&B) 21493687 R
TN0041A tCGCCACCATggtgagcaagggcgagGAGCTGTTCa
aGCGGTGGTAccactcgttcccgctcCTCGACAAGt
    9+[16]+9
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041B tGGTGCCCATcctggtcgagctggacGGCGACGTAa
aCCACGGGTAggaccagctcgacctgCCGCTGCATt
    9+[16]+9
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041C tCTGCACCACcggcaagctgcccgtgCCCTGGCCCa
aGACGTGGTGgccgttcgacgggcacGGGACCGGGt
    9+[16]+9
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041D tGGAGTACAActacaacagccacaacGTCTATATCa
aCCTCATGTTgatgttgtcggtgttgCAGATATAGt
    9+[16]+9
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041E tTCAGCGTGTCcggcgagggcgagggcGATGCCACCTa
aAGTCGCACAGgccgctcccgctcccgCTACGGTGGAt
    10+[16]+10
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041F tGCCACCTACGgcaagctgaccctgaaGTTCATCTGCa
aCGGTGGATGCcgttcgactgggacttCAAGTAGACGt
    10+[16]+10
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041G tGGCCCACCCTcgtgaccaccctgaccTACGGCGTGCa
aCCGGGTGGGAgcactggtgggactggATGCCGCACGt
    10+[16]+10
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041H tTCAAGATCCGccacaacatcgaggacGGCAGCGTGCa
aAGTTCTAGGCggtgttgtagctcctgCCGTCGCACGt
    10+[16]+10
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041I tAGAGGATCCACcggtcgccaccatggtGAGCAAGGGCGa
aTCTCCTAGGTGgccagcggtggtaccaCTCGTTCCCGCt
    11+[16]+11
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041J tCCGGCGAGGGCgagggcgatgccacctACGGCAAGCTGa
aGGCCGCTCCCGctcccgctacggtggaTGCCGTTCGACt
    11+[16]+11
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041K tGACCTACGGCGtgcagtgcttcagccgCTACCCCGACCa
aCTGGATGCCGCacgtcacgaagtcggcGATGGGGCTGGt
    11+[16]+11
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041L tCCGCCACAACAtcgaggacggcagcgtGCAGCTCGCCGa
aGGCGGTGTTGTagctcctgccgtcgcaCGTCGAGCGGCt
    11+[16]+11
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041M tCCTGGTCGAGCTggacggcgacgtaaacGGCCACAAGTTCa
aGGACCAGCTCGAcctgccgctgcatttgCCGGTGTTCAAGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041N tCAGCCGCTACCCcgaccacatgaagcagCACGACTTCTTCa
aGTCGGCGATGGGgctggtgtacttcgtcGTGCTGAAGAAGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041O tCTTCAAGTCCGCcatgcccgaaggctacGTCCAGGAGCGCa
aGAAGTTCAGGCGgtacgggcttccgatgCAGGTCCTCGCGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041P tCAAGGAGGACGGcaacatcctggggcacAAGCTGGAGTACa
aGTTCCTCCTGCCgttgtaggaccccgtgTTCGACCTCATGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041Q tCAAGGTGAACTTcaagatccgccacaacATCGAGGACGGCa
aGTTCCACTTGAAgttctaggcggtgttgTAGCTCCTGCCGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041R tCCACCGGTCGCCAccatggtgagcaagggCGAGGAGCTGTTCa
aGGTGGCCAGCGGTggtaccactcgttcccGCTCCTCGACAAGt
    13+[16]+13
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041S tTCAGCGTGTCCGGcgagggcgagggcgatGCCACCTACGGCAa
aAGTCGCACAGGCCgctcccgctcccgctaCGGTGGATGCCGTt
    13+[16]+13
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041T tTCAGCCGCTACCCcgaccacatgaagcagCACGACTTCTTCAa
aAGTCGGCGATGGGgctggtgtacttcgtcGTGCTGAAGAAGTt
    13+[16]+13
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041U tACAACAGCCACAAcgtctatatcatggccGACAAGCAGAAGAa
aTGTTGTCGGTGTTgcagatatagtaccggCTGTTCGTCTTCTt
    13+[16]+13
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041V tGGTGCCCATCCTGGtcgagctggacggcgaCGTAAACGGCCACAa
aCCACGGGTAGGACCagctcgacctgccgctGCATTTGCCGGTGTt
    14+[16]+14
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041W tGCCCGAAGGCTACGtccaggagcgcaccatCTTCTTCAAGGACGa
aCGGGCTTCCGATGCaggtcctcgcgtggtaGAAGAAGTTCCTGCt
    14+[16]+14
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041X tGAACCGCATCGAGCtgaagggcatcgacttCAAGGAGGACGGCAa
aCTTGGCGTAGCTCGacttcccgtagctgaaGTTCCTCCTGCCGTt
    14+[16]+14
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041Y tCAAGATCCGCCACAacatcgaggacggcagCGTGCAGCTCGCCGa
aGTTCTAGGCGGTGTtgtagctcctgccgtcGCACGTCGAGCGGCt
    14+[16]+14
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041Z tGTTCACCGGGGTGGTgcccatcctggtcgagCTGGACGGCGACGTAa
aCAAGTGGCCCCACCAcgggtaggaccagctcGACCTGCCGCTGCATt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AA tGCCCATCCTGGTCGAgctggacggcgacgtaAACGGCCACAAGTTCa
aCGGGTAGGACCAGCTcgacctgccgctgcatTTGCCGGTGTTCAAGt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AB tGTCCGGCGAGGGCGAgggcgatgccacctacGGCAAGCTGACCCTGa
aCAGGCCGCTCCCGCTcccgctacggtggatgCCGTTCGACTGGGACt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AC tCATCTGCACCACCGGcaagctgcccgtgcccTGGCCCACCCTCGTGa
aGTAGACGTGGTGGCCgttcgacgggcacgggACCGGGTGGGAGCACt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AD tCTATATCATGGCCGAcaagcagaagaacggcATCAAGGTGAACTTCa
aGATATAGTACCGGCTgttcgtcttcttgccgTAGTTCCACTTGAAGt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AE tACGGCGTGCAGTGCTTcagccgctaccccgacCACATGAAGCAGCACGa
aTGCCGCACGTCACGAAgtcggcgatggggctgGTGTACTTCGTCGTGCt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AF tACCCCGACCACATGAAgcagcacgacttcttcAAGTCCGCCATGCCCGa
aTGGGGCTGGTGTACTTcgtcgtgctgaagaagTTCAGGCGGTACGGGCt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AG tCCGCCATGCCCGAAGGctacgtccaggagcgcACCATCTTCTTCAAGGa
aGGCGGTACGGGCTTCCgatgcaggtcctcgcgTGGTAGAAGAAGTTCCt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AH tTCAAGGAGGACGGCAAcatcctggggcacaagCTGGAGTACAACTACAa
aAGTTCCTCCTGCCGTTgtaggaccccgtgttcGACCTCATGTTGATGTt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AI tACAACTACAACAGCCAcaacgtctatatcatgGCCGACAAGCAGAAGAa
aTGTTGATGTTGTCGGTgttgcagatatagtacCGGCTGTTCGTCTTCTt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AJ tCACCGGGGTGGTGCCCAtcctggtcgagctggaCGGCGACGTAAACGGCCa
aGTGGCCCCACCACGGGTaggaccagctcgacctGCCGCTGCATTTGCCGGt
    17+[16]+17
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AK tAAACGGCCACAAGTTCAgcgtgtccggcgagggCGAGGGCGATGCCACCTa
aTTTGCCGGTGTTCAAGTcgcacaggccgctcccGCTCCCGCTACGGTGGAt
    17+[16]+17
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AL tGCAGTGCTTCAGCCGCTaccccgaccacatgaaGCAGCACGACTTCTTCAa
aCGTCACGAAGTCGGCGAtggggctggtgtacttCGTCGTGCTGAAGAAGTt
    17+[16]+17
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AM tCAGCGTGTCCGGCGAGGGcgagggcgatgccaccTACGGCAAGCTGACCCTGa
aGTCGCACAGGCCGCTCCCgctcccgctacggtggATGCCGTTCGACTGGGACt
    18+[16]+18
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AN tGAAGTTCATCTGCACCACcggcaagctgcccgtgCCCTGGCCCACCCTCGTGa
aCTTCAAGTAGACGTGGTGgccgttcgacgggcacGGGACCGGGTGGGAGCACt
    18+[16]+18
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AO tCGTGACCACCCTGACCTAcggcgtgcagtgcttcAGCCGCTACCCCGACCACa
aGCACTGGTGGGACTGGATgccgcacgtcacgaagTCGGCGATGGGGCTGGTGt
    18+[16]+18
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AP tCATGGCCGACAAGCAGAAgaacggcatcaaggtgAACTTCAAGATCCGCCACa
aGTACCGGCTGTTCGTCTTcttgccgtagttccacTTGAAGTTCTAGGCGGTGt
    18+[16]+18
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AQ tTCACCGGGGTGGTGCCCATc...cGGCGACGTAAACGGCCACAa
aAGTGGCCCCACCACGGGTAg...gCCGCTGCATTTGCCGGTGTt
    19+[16]+19
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AR tGCACCACCGGCAAGCTGCCc...cCTCGTGACCACCCTGACCTa
aCGTGGTGGCCGTTCGACGGg...gGAGCACTGGTGGGACTGGAt
    19+[16]+19
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AS tTCAAGTCCGCCATGCCCGAa...gCGCACCATCTTCTTCAAGGa
aAGTTCAGGCGGTACGGGCTt...cGCGTGGTAGAAGAAGTTCCt
    19+[16]+19
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AT tATATCATGGCCGACAAGCAg...gGTGAACTTCAAGATCCGCCa
aTATAGTACCGGCTGTTCGTc...cCACTTGAAGTTCTAGGCGGt
    19+[16]+19
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AU tTCATCTGCACCACCGGCAAGc...cCCACCCTCGTGACCACCCTGa
aAGTAGACGTGGTGGCCGTTCg...gGGTGGGAGCACTGGTGGGACt
    20+[16]+20
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AV tGAAGTTCGAGGGCGACACCCt...aGCTGAAGGGCATCGACTTCAa
aCTTCAAGCTCCCGCTGTGGGa...tCGACTTCCCGTAGCTGAAGTt
    20+[16]+20
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0158A ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    19+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158B ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATcgatggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAgctacccctag
    19+[17]+11
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158C ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCgatggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGctacccctag
    19+[17]+12
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158D ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGatggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCtacccctag
    19+[17]+13
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158E ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGAtggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTacccctag
    19+[17]+14
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158F ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTAcccctag
    19+[17]+15
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158G ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCcctag
    19+[17]+17
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158H ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGgatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCctag
    19+[17]+18
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158I ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGAtc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCTag
    19+[17]+20
eGFP [mutated] G (std.) WT ICA HR 22740649 R
TN0158J ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGATc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCTAg
    19+[17]+21
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158K ntccagtagcAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtcatcgTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    11+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158L ntccagtagCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtcatcGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    12+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158M ntccagtaGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtcatCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    13+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158N ntccagtAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtcaTCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    14+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158O ntccaGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    16+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158P ntccAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    17+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158Q ntcCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
nagGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    18+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158R nTCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
nAGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    20+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
GSearch EENdb with this gene symbol
RSearch EENdb with this reference

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