EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
All EENs reported with specificity information: 100 ZFN, 14 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0025 tCCCCTCCACCCCACAGTggggccactagggacAGGATTGGTGACAGAAAa
aGGGGAGGTGGGGTGTCAccccggtgatccctgTCCTAACCACTGTCTTTt
    17+[15]+17
human, PPP1R12C, intron G C (std.) +63 WT; ELD+KKR (N/A) NHEJ, HR 21738127 R
TN0025-T002 tTCCACCCAGCCCTCAGTgctctgctgaggcAGGATGGGTGGCTGGAAa
aAGGTGGGTCGGGAGTCAcgagacgactccgTCCTACCCACCGACCTTt
    17+[13]+17
human, [intergenic] C +63 ELD+KKR (N/A) NHEJ 21738127 R
TN0025-T003 cCCCCTCCACCCCAAAAAt...aAATATGAGTTATAGAAAa
gGGGGAGGTGGGGTTTTTa...tTTATACTCAATATCTTTt
    17+[24]+17
human, [intergenic] C +63 ELD+KKR (N/A) NHEJ 21738127 R
TN0029 tCTGGGCTCTCCCATgcattcaaactgaggtgcctgcCCTTCTAGGAATGGGGGa
aGACCCGAGAGGGTAcgtaagtttgactccacggacgGGAAGATCCTTACCCCCt
    14+[22]+17
human, POU5F1, 3'-UTR G C (std.) +63 WT (N/A) NHEJ, HR 21738127 R
TN0031 tGTGGGCAACATGCTGGTCatcctcatcctgataAACTGCAAAAGGCTGAAGa
aCACCCGTTGTACGACCAGtaggagtaggactatTTGACGTTTTCCGACTTCt
    18+[15]+18
human, CCR5 G C (std.) +47, +17 KV+EA (N/A) NHEJ 21813459 R
TN0031-T002 tGTGGGCAACATGCTGGTCgtcctcatcttaataAACTGCAAAAAGCTGAAGt
aCACCCGTTGTACGACCAGcaggagtagaattatTTGACGTTTTTCGACTTCa
    18+[15]+18
human, CCR2 G C +47, +17 KV+EA (N/A) NHEJ 21813459 R Low
TN0032 tTCCTGCCCAGCTCCATttccttctcctggaactACCAGAACAACACTGAa
aAGGACGGGTCGAGGTAaaggaagaggaccttgaTGGTCTTGTTGTGACTt
    16+[17]+16
rat, IGG2B G (std.) +63 ELD+KKR (N/A) NHEJ 21822240 R
TN0032-T002 tTCCTGACCAGCTCCAAgcataaaagcatAACAGTAGAAAATTGAt
aAGGACTGGTCGAGGTTcgtattttcgtaTTGTCATCTTTTAACTa
    16+[12]+16
rat, [intergenic] +63 ELD+KKR (N/A) NHEJ 21822240 R
TN0035 tGTTATTTTCTCCCCTaaggatcctgcgggcATTTTTGAGCTGGTGGa
aCAATAAAAGAGGGGAttcctaggacgcccgTAAAAACTCGACCACCt
    15+[15]+16
zebrafish, tnikb G C (std.) +63 Sharkey-AS+R UA NHEJ 21822242 R
TN0035B tGTTATTTTCTCCCCTaaggatcctgcgggcATTTTTGAGCTGGTGGa
aCAATAAAAGAGGGGAttcctaggacgcccgTAAAAACTCGACCACCt
    15+[15]+16
zebrafish, tnikb G C (NK) +63 Sharkey-AS+R UA NHEJ 21822242 R
TN0150 tACAATACTCCCACTGAAatgacagatgcagacagACTCATGGACAGTTGGTa
aTGTTATGAGGGTGACTTtactgtctacgtctgtcTGAGTACCTGTCAACCAt
    17+[17]+17
zebrafish, epas1b G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0150-T002 tACGCTACTCCCACTGAAatgacagatgcagacagACTCATGGACAGTTGGTa
aTGCGATGAGGGTGACTTtactgtctacgtctgtcTGAGTACCTGTCAACCAt
    17+[17]+17
zebrafish, epas1b [mutated] G C +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0162 tGGACAATTCATCCGGCATccaagcagaggtcagAGGGTGAGAAGGTGCGCa
aCCTGTTAAGTAGGCCGTAggttcgtctccagtcTCCCACTCTTCCACGCGt
    18+[15]+17
zebrafish, ryr3 G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0164 tGGACCATTCACCCTGCATccaagcagaggtcagAGGGTGAGAAGGTCAGa
aCCTGGTAAGTGGGACGTAggttcgtctccagtcTCCCACTCTTCCAGTCt
    18+[15]+16
zebrafish, ryr1a G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZNA005
(Z-M)
GGGGCGGAAn
CCCCGCCTTn
    3F
(artificial) ZP00009 QNKKGLD(G4S)3 WT 9862996 R
ZNA005-T002
(Z-M)
GGGGCAGAAn
CCCCGTCTTn
    3F
(artificial) ZP00009-T002 QNKKGLD(G4S)3 WT 9862996 R
ZNA005-T003
(Z-M)
GGGTCAGAAn
CCCAGTCTTn
    3F
(artificial) ZP00009-T003 QNKKGLD(G4S)3 WT 9862996 R
ZN0016 tCTCGACCCTAATgcaatgTACTCGGTCCTGc
aGAGCTGGGATTAcgttacATGAGCCAGGACg
    4F+[6]+4F
zebrafish, ntla G C ZP10043 ZP10044, ZP10045 LRGS WT Sangamo NHEJ 18500334 R
ZN0017 aCACACCTTCagcatgTTGGTGGGAc
tGTGTGGAAGtcgtacAACCACCCTg
    3F+[6]+3F
zebrafish, kdrl G C ZP10046 ZP10047 TGGS D+R PRS NHEJ 18500337 R
ZN0018 cCTACTCaTTCAACcaataGCCCGGGCCGTAc
gGATGAGtAAGTTGgttatCGGGCCCGGCATg
    (2F+1+2F)+[5]+4F
human, (mtDNA) G C ZP10051, ZP10052; ZP10053 - ZP10057 ZP10061, ZP10062; ZP10063 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018-T002 cCTACTCaTTCAACcaataGCCCTGGCCGTAc
gGATGAGtAAGTTGgttatCGGGACCGGCATg
    (2F+1+2F)+[5]+4F
human, (mtDNA) G C ZP10053 - ZP10057 ZP10061-T002 - ZP10063-T002 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018B cAGCCTACTCaTTCAACcaataGCCCGGGCCGTAc
gTCGGATGAGtAAGTTGgttatCGGGCCCGGCATg
    (2F+1+3F)+[5]+4F
human, (mtDNA) G C ZP10058 ZP10061 - ZP10063 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018B-T002 cAGCCTACTCaTTCAACcaataGCCCTGGCCGTAc
gTCGGATGAGtAAGTTGgttatCGGGACCGGCATg
    (2F+1+3F)+[5]+4F
human, (mtDNA) G C ZP10058 ZP10061-T002 - ZP10063-T002 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018C
(Z-S1)
GCCCGGGCCGTAc
CGGGCCCGGCATg
    4F
human, (mtDNA) [mutated] G C ZP10063 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0019 gGTCATCCTCATCctgatAAACTGCAAAAGg
cCAGTAGGAGTAGgactaTTTGACGTTTTCc
    4F+[5]+4F
human, CCR5 G C ZP10064 ZP10065 LRGS WT, EL+KK Sangamo NHEJ 18587387 R
ZN0019-T002 gGTCGTCCTCATCttaatAAACTGCAAAAAg
cCAGCAGGAGTAGaattaTTTGACGTTTTTc
    4F+[5]+4F
human, CCR2 G C ZP10064-T002 ZP10065-T002 LRGS EL+KK Sangamo NHEJ 18587387 R
ZN0019-T003 aGTCATCCTCATCtcacgGATGAGGATGCCa
tCAGTAGGAGTAGagtgcCTACTCCTACGGt
    4F+[5]+4F
human, ABLIM2, intron G C ZP10064 ZP10064-T003 LRGS EL+KK Sangamo NHEJ 18587387 R Low
ZN0028 gGAATCCTTCtaatgaGGCGGAGATc
cCTTAGGAAGattactCCGCCTCTAg
    3F+[6]+3F
tobacco, SuRB/SuRA G ZP10335 ZP10337 LRGS WT OPEN NHEJ 18657511 R
ZNA014 nTGCATCCATnnnnnnATGGATGCAn
nACGTAGGTAnnnnnnTACCTACGTn
    3F+[6]+3F
(artificial) ZP00121 ZP00121 (N/A) WT MA 18725642 R
ZN0037 cTACCACGTCacgagcAGGGTGGTGt
gATGGTGCAGtgctcgTCCCACCACa
    3F+[6]+3F
tobacco, SuRB G ZP10392 ZP10393 LRGS WT, D+R MA NHEJ; HR 19404258 R
ZN0037-T002 cTGCCACGTCacgagcAGGGCGGTGt
gACGGTGCAGtgctcgTCCCGCCACa
    3F+[6]+3F
tobacco, SuRA G ZP10392-T002 ZP10393-T002 LRGS WT, D+R MA NHEJ; HR 19404258 R
ZN0038 gGGCAGCACCagatgTGGGCTGCTc
cCCGTCGTGGtctacACCCGACGAg
    3F+[5]+3F
tobacco, SuRB G ZP10400 ZP10406 LRGS EL+KK OPEN NHEJ; HR 19404258 R
ZN0038-T002 gGGCAACACCagatgTGGGCTGCTc
cCCGTTGTGGtctacACCCGACGAg
    3F+[5]+3F
tobacco, SuRA G ZP10400-T002 ZP10406 LRGS EL+KK OPEN NHEJ; HR 19404258 R
ZN0039 cCTGTGGGGCCATatcccaGAACTGGTTGAGTCGGTCa
gGACACCCCGGTAtagggtCTTGACCAACTCAGCCAGt
    4F+[6]+6F
maize, ipp2K G ZP10429 ZP10430, ZP10431 LRGS EL+KK Sangamo NHEJ; HR 19404259 R
ZN0039-T002 cCTGTGGGGCCATattccaGAACTGGTTGAGTCTGTCa
gGACACCCCGGTAtaaggtCTTGACCAACTCAGACAGt
    4F+[6]+6F
maize, LOC100126363 G ZP10429 ZP10431-T002 LRGS EL+KK Sangamo NHEJ; HR 19404259 R
ZN0040 cCAACACCTGGGTGCCaatcatGTCGATGGTGGGGTATGGt
gGTTGTGGACCCACGGttagtaCAGCTACCACCCCATACCa
    5F+[6]+6F
maize, ipp2K G ZP10432; ZPU0001 ZP10433; ZPU0002 LRGS EL+KK Sangamo NHEJ; HR 19404259 R
ZN0045 cTTCTTCATCATCctcctGACAATCGAt
gAAGAAGTAGTAGgaggaCTGTTAGCTa
    4F+[5]+3F
human, CCR5/CCR2 G C ZP10442 ZP10443 TGEK WT MA NHEJ 19470664 R
ZN0046 cTGTGTTTGCTTTaaaagcCAGGACGGTCACc
gACACAAACGAAAttttcgGTCCTGCCAGTGg
    4F+[6]+4F
human, CCR5/CCR2 G C ZP10444 ZP10445 TGEK WT MA NHEJ 19470664 R
ZN0048 aCGGTCACCTttggggTGGTGACAAGTGt
tGCCAGTGGAaaccccACCACTGTTCACa
    3F+[6]+4F
human, CCR5/CCR2 G C ZP10448 ZP10449 TGEK WT MA NHEJ 19470664 R
ZN0050 cCCCATCATCTATgcctttGTCGGGGAGAAGt
gGGGTAGTAGATAcggaaaCAGCCCCTCTTCa
    4F+[6]+4F
human, CCR5 G C ZP10452 ZP10453 TGEK WT, D+R, EL+KK MA NHEJ 19470664 R
ZN0050-T002 cCCCATCATCTATgccttCGTTGGGGAGAAGt
gGGGTAGTAGATAcggaaGCAACCCCTCTTCa
    4F+[5]+4F
human, CCR2 G C ZP10452 ZP10453-T002 TGEK WT, D+R, EL+KK MA NHEJ 19470664 R Low
ZN0052 cAACTACAACAGCCACAACgtctatATCATGGCCGACAAGc
gTTGATGTTGTCGGTGTTGcagataTAGTACCGGCTGTTCg
    6F+[6]+5F
eGFP G ZP10458 ZP10459 LRGS WT Sangamo NHEJ 19628861 R
ZN0053 cGAGAGCCCCCTGTCTgatgagAATTTGGTGGCCATGGGCt
gCTCTCGGGGGACAGActactcTTAAACCACCGGTACCCGa
    5F+[6]+6F
rat, IGG2B G ZP10460 ZP10461 LRGS EL+KK Sangamo NHEJ 19628861 R
ZN0055 tACTGTCCCCTTCtgggctcactatGCTGCCGCCCAGt
aTGACAGGGGAAGacccgagtgataCGACGGCGGGTCa
    4F+[12]+4F
human, CCR5 G C ZP10465 ZP10464 QNKKGLD(G4S)3 WT, D+R, EL+KK MA NHEJ 19635463 R
ZN0056 aGCTCTCATTTTCcatacaGTCAGTATCAATt
tCGAGAGTAAAAGgtatgtCAGTCATAGTTAa
    4F+[6]+4F
human, CCR5 G C ZP10467 ZP10466 QNKK WT, D+R, EL+KK MA NHEJ 19635463 R
ZN0057 cACCCCACAGTGGggccacTAGGGACAGGATt
gTGGGGTGTCACCccggtgATCCCTGTCCTAa
    4F+[6]+4F
human, PPP1R12C, intron G C ZP10468 ZP10469 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0058 cCAGACCtGGCACCCAGgagagGAGCAGGCAGGGTCAGCTg
gGTCTGGaCCGTGGGTCctctcCTCGTCCGTCCCAGTCGAc
    (3F+1+2F)+[5]+6F
human, POU5F1, intron G C ZP10470 ZP10471 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0060 aGTAGTCCTTTGTTACatgcatGAGTCAGTGAACAGGg
tCATCAGGAAACAATGtacgtaCTCAGTCACTTGTCCc
    5F+[6]+5F
human, POU5F1, intron G C ZP10474 ZP10475 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0061 cCCCAGCTCCCAGAGCacggctGCAAGGGCCaGGAGCAc
gGGGTCGAGGGTCTCGtgccgaCGTTCCCGGtCCTCGTg
    5F+[6]+(3F+1+2F)
human, PITX3 G C ZP10477 ZP10476 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0061-T002 tAGCTCACaGTCCCTTGT AAAAGGGCCCaGGGAGCAg
aTCGAGTGtCAGGGAACA TTTTCCCGGGtCCCTCGTc
    5F+5F
human, PKP1, intron G C ZP10477-T002 ZP10477-T003 LRGS EL+KK Sangamo NHEJ 19680244 R Low
ZN0075 aCCAACCTCACTATGCactataGGTATGAGAAGGGGGa
tGGTTGGAGTGATACGtgatatCCATACTCTTCCCCCt
    5F+[6]+5F
rat, Il2rg, exon-intron G ZPU0005 ZPU0006 LRGS EL+KK Sangamo NHEJ 20111598 R
ZN0138B gGGAGTGgTGCATGGTGtggcaaCAGGTAaGCTCCAtTGTGCTt
cCCTCACcACGTACCACaccgttGTCCATtCGAGGTaACACGAa
    (3F+1+2F)+[6]+(2F+1+2F+1+2F)
human, SNCA G C ZP10755, ZP10756 ZP10759 WT, ELD+KKR Sangamo NHEJ; HR 20120421 R
ZN0078 tATCTTCGGCcatgaaGCTGGAGGGt
aTAGAAGCCGgtacttCGACCTCCCa
    3F+[6]+3F
thale cress, ADH1 G ZP10502 ZP10508 LRGS WT, EL+KK OPEN NHEJ 20508152 R
ZN0079 cGGCGTCGACatgcctGGTGCTGACt
gCCGCAGCTGtacggaCCACGACTGa
    3F+[6]+3F
thale cress, TT4 G ZP10511 ZP10525 LRGS WT, EL+KK OPEN NHEJ 20508152 R
ZN0081 gGCCATCAGCCCTgttcttGGACTGTCAGCTGGTa
cCGGTAGTCGGGAcaagaaCCTGACAGTCGACCAt
    4F+[6]+5F
mouse, Abcb1a G ZP10553 ZP10554 LRGS WT Sangamo NHEJ 20628038 R
ZN0082 tTCCTTCCTCGCTGGCaggtgTGCGGGGCCTCGGGTc
aAGGAAGGAGCGACCGtccacACGCCCCGGAGCCCAg
    5F+[5]+5F
mouse, Jag1, intron-exon G ZPU0011 ZPU0012 LRGS WT Sangamo NHEJ 20628038 R
ZN0083 tGACTGCTCTCCGGATCcctgccACCACGGGCGCTGTGt
aCTGACGAGAGGCCTAGggacggTGGTGCCCGCGACACa
    (5F+1)+[6]+5F
mouse, Notch3 G ZPU0013 ZPU0014 LRGS WT Sangamo NHEJ 20628038 R
ZN0081B gGCCATCAGCCCTgttcttGGACTGTCAGCTGGTa
cCGGTAGTCGGGAcaagaaCCTGACAGTCGACCAt
    4F+[6]+5F
rat, Abcb1a G ZP10553 ZP10554 LRGS WT Sangamo NHEJ 20628038 R
ZN0017B aCACACCTTCagcatgTTGGTGGGAc
tGTGTGGAAGtcgtacAACCACCCTg
    3F+[6]+3F
zebrafish, kdrl G C ZP10048 ZP10552 TGGS D+R, EL+KK PRS & MA NHEJ 20843781 R
ZN0017-T002 aTCCCACCCGagtcctGCAGGTGTGt
tAGGGTGGGCtcaggaCGTCCACACa
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10047-T002 ZP10046-T002 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T003 aCACACCATCctacctTTGGTGGGTa
tGTGTGGTAGgatggaAACCACCCAt
    3F+[6]+3F
zebrafish, -, intron G C ZP10046-T003 ZP10047-T003 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T004 aCACACCTTCacagacGTGGGGGGAa
tGTGTGGAAGtgtctgCACCCCCCTt
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10046 ZP10047-T004 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T005 tCCCGACCAGattgtGAAGGTGTGt
aGGGCTGGTCtaacaCTTCCACACa
    3F+[5]+3F
zebrafish, fhdc1 G C ZP10047-T005 ZP10046 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T006 aACCCACCGAgatacGCGGGTGTGt
tTGGGTGGCTctatgCGCCCACACa
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10047-T006 ZP10046-T004 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T007 aCCCACCCTCgtgatgTTGGAGGGAa
tGGGTGGGAGcactacAACCTCCCTt
    3F+[6]+3F
zebrafish, odf3b G C ZP10046-T005 ZP10047-T007 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T008 cCACACCGGCagactGCGGCGGGAt
gGTGTGGCCGtctgaCGCCGCCCTa
    3F+[5]+3F
zebrafish, tmpoa G C ZP10046-T006 ZP10047-T008 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T009 cCACACCCACaaaagATGGTGGGTc
gGTGTGGGTGttttcTACCACCCAg
    3F+[5]+3F
zebrafish, tanc2, intron G C ZP10046-T007 ZP10047-T009 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T010 cTCCCACCCAggaagtGATGGTGAGt
gAGGGTGGGTccttcaCTACCACTCa
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10047-T010 ZP10046-T008 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T011 aTCCCACCGGagcggtGATGGTGAAc
tAGGGTGGCCtcgccaCTACCACTTg
    3F+[6]+3F
zebrafish, - G C ZP10047-T011 ZP10046-T009 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T012 gTCCCGCCAAcaaatGACGGAGTGc
cAGGGCGGTTgtttaCTGCCTCACg
    3F+[5]+3F
zebrafish, -, intron G C ZP10047-T012 ZP10046-T010 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T013 gCGCACCGCCagacatATGGTGGGAt
cGCGTGGCGGtctgtaTACCACCCTa
    3F+[6]+3F
zebrafish, sox6, intron G C ZP10046-T011 ZP10047-T013 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T014 aTCCCCCCTGccatgaGGAGGTGTGt
tAGGGGGGACggtactCCTCCACACa
    3F+[6]+3F
zebrafish, -, intron G C ZP10047-T014 ZP10046-T012 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T015 cACCCACCCActactGAGGGTGAGc
gTGGGTGGGTgatgaCTCCCACTCg
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10047-T015 ZP10046-T013 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T016 tCACACCTCCaattaGAGGCGGGAt
aGTGTGGAGGttaatCTCCGCCCTa
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10046-T012 ZP10047-T016 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T017 gTCCCTCCCTaagggtGATGGGGTGt
cAGGGAGGGAttcccaCTACCCCACa
    3F+[6]+3F
zebrafish, adcyap1b, 3'-UTR G C ZP10047-T017 ZP10046-T014 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T018 gCACACCAGCtgcattTTGGTGGGTc
cGTGTGGTCGacgtaaAACCACCCAg
    3F+[6]+3F
zebrafish, - G C ZP10046-T015 ZP10047-T003 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T019 cTTCCACCAAgtatcaGAAGGTGTAc
gAAGGTGGTTcatagtCTTCCACATg
    3F+[6]+3F
zebrafish, ctnna2 G C ZP10047-T018 ZP10046-T016 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T020 cTCCCACCAGgatatccgggttacGCAGGTGTGg
gAGGGTGGTCctataggcccaatgCGTCCACACc
    3F+[14]+3F
zebrafish, myo5aa G C ZP10047-T019 ZP10046-T002 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZNA014-T002 cAGCATCCATgtaagtATGGATGCTg
gTCGTAGGTAcattcaTACCTACGAc
    3F+[6]+3F
(artificial) ZP00121-T002 ZP00121-T002 (N/A) WT MA 21029755 R Low
ZNA014-T003 cGGCATCCATgtaagtATGGATGCCg
gCCGTAGGTAcattcaTACCTACGGc
    3F+[6]+3F
(artificial) ZP00121-T003 ZP00121-T003 (N/A) WT MA 21029755 R
ZNA014-T004 cTGCATCCCTgtaagtAGGGATGCAg
gACGTAGGGAcattcaTCCCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T004 ZP00121-T004 (N/A) WT MA 21029755 R Low
ZNA014-T005 cTGCATCCGTgtaagtACGGATGCAg
gACGTAGGCAcattcaTGCCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T005 ZP00121-T005 (N/A) WT MA 21029755 R
ZNA014-T006 cTGCATCCTTgtaagtAAGGATGCAg
gACGTAGGAAcattcaTTCCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T006 ZP00121-T006 (N/A) WT MA 21029755 R
ZNA014-T007 cTGCATCCACgtaagtGTGGATGCAg
gACGTAGGTGcattcaCACCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T007 ZP00121-T007 (N/A) WT MA 21029755 R
ZNA014-T008 cAGCATCCTCgtaagtGAGGATGCTg
gTCGTAGGAGcattcaCTCCTACGAc
    3F+[6]+3F
(artificial) ZP00121-T008 ZP00121-T008 (N/A) WT MA 21029755 R
ZNA014-T009 cTGCATCCATgtaagtATGGCTGCAg
gACGTAGGTAcattcaTACCGACGTc
    3F+[6]+3F
(artificial) ZP00121 ZP00121-T009 (N/A) WT MA 21029755 R
ZNA014-T010 cTGCATCCATgtaagtATGGACGCAg
gACGTAGGTAcattcaTACCTGCGTc
    3F+[6]+3F
(artificial) ZP00121 ZP00121-T010 (N/A) WT MA 21029755 R
ZN0093 tTGCTTCATCacaatGGAGATGATt
aACGAAGTAGtgttaCCTCTACTAa
    3F+[5]+3F
soybean, Dcl4a/Dcl4b G ZP10588 ZP10638 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0118 cCTTATTgAGCATCCGGATCctatcTATGATCCCAAGGAGAAGg
gGAATAAcTCGTAGGCCTAGgatagATACTAGGGTTCCTCTTCc
    (4F+1+2F)+[5]+6F
frog, nog G ZP10713, ZP10714 ZP10711, ZP10712 LRGS EL+KK Sangamo NHEJ 21471457 R
ZN0119 cATCCCATTCCCGAGAgctgatCCAATGGTTGCAGATt
gTAGGGTAAGGGCTCTcgactaGGTTACCAACGTCTAa
    5F+[6]+5F
pig, PPARG G ZPU0021 ZPU0023 LRGS EL+KK Sangamo NHEJ 21502977 R
ZN0119-T002 cATTCCATTCCCCAGAtataaCCACTGTTTGGAGTTt
gTAAGGTAAGGGGTCTatattGGTGACAAACCTCAAa
    5F+[5]+5F
pig, CASP10, intron G ZPU0021-T002 ZPU0023-T002 LRGS EL+KK Sangamo NHEJ 21502977 R
ZN0119-T003 tATGGCATTGCCGTGAgttgcgCTATAGGTTGCAGATg
aTACCGTAACGGCACTcaacgcGATATCCAACGTCTAc
    5F+[6]+5F
pig, (intergenic) G ZPU0021-T003 ZPU0023-T003 LRGS EL+KK Sangamo NHEJ 21502977 R
ZN0120 tGACCGCTTCTACcccaatGACTTGTGGGTGg
aCTGGCGAAGATGgggttaCTGAACACCCACc
    4F+[6]+4F
human, CXCR4 G C ZP10715 ZP10716 LRGS EL+KK Sangamo NHEJ 21533216 R
ZN0120-T002 cCACCCACAGGTTttaaggGTAGAAGCAGCAa
gGTGGGTGTCCAAaattccCATCTTCGTCGTt
    4F+[6]+4F
human, ADAMTS17, intron G C ZP10715-T002 ZP10716-T002 LRGS EL+KK Sangamo NHEJ 21533216 R Low
ZN0120-T003 cCACCTACTTGTCttgtgtGTTGAAGCAGACa
gGTGGATGAACAGaacacaCAACTTCGTCTGt
    4F+[6]+4F
human, (intergenic) G C ZP10715-T003 ZP10716-T003 LRGS EL+KK Sangamo NHEJ 21533216 R Low
ZN0133 cAGCCTGATGgAACTTAtaagggAGAAAGtGATTTGCAGt
gTCGGACTACcTTGAATattcccTCTTTCaCTAAACGTCa
    (2F+1+3F)+[6]+(2F+1+3F)
worm, ben-1 G ZP10743 ZP10744 (N/A) WT, ELD+KKR(?) Sangamo NHEJ 21700836 R
ZN0135 cCCGAGCtCACATCGATgatcaaGAAAACACGGCAGCAa
gGGCTCGaGTGTAGCTActagttCTTTTGTGCCGTCGTt
    (3F+1+2F)+[6]+5F
worm, Cbr-sdc-2 G ZP10749 ZP10750 (N/A) WT Sangamo NHEJ 21700836 R
ZN0136 gTGCCAGGTACTGTGTcagggtACTAGGGGTATGg
cACGGTCCATGACACAgtcccaTGATCCCCATACc
    5F+[6]+4F
human, F9, intron G C ZP10751 ZP10752 LRGS WT Sangamo NHEJ; HR 21706032 R
ZN0136-T002 aAGGCAGGTGCTGTGTaagtacACTAAGGATGTGg
tTCCGTCCACGACACAttcatgTGATTCCTACACc
    5F+[6]+4F
mouse, (intergenic) G ZP10751-T002 ZP10752-T002 LRGS WT Sangamo NHEJ 21706032 R Low
ZN0137 tCGGTGGCTCAGCTACaggcctGGTGGTACAAGGCACa
aGCCACCGAGTCGATGtccggaCCACCATGTTCCGTGt
    5F+[6]+5F
pig, GGTA1 G ZP10753 ZP10754 LRGS EL+KK Sangamo NHEJ 21730124 R
ZN0138 gGGAGTGgTGCATGGTGtggcaaCAGGTAaGCTCCATTGTGCtt
cCCTCACcACGTACCACaccgttGTCCATtCGAGGTAACACGaa
    (3F+1+2F)+[6]+(2F+1+4F)
human, SNCA G C ZP10755, ZP10756 ZP10757, ZP10758; ZP10759 LRGS WT, ELD+KKR Sangamo NHEJ; HR 21757228 R
ZN0144 aGTCTGCCGTTACtgccctGTGGGGCAAGGTGAACGTGg
tCAGACGGCAATGacgggaCACCCCGTTCCACTTGCACc
    4F+[6]+(1+6F)
human, HBB G C ZPU0031 ZPU0032 (N/A) (N/A) Sangamo HR 21881051 R
ZN0145 cCGTTACTGCcctgtGGGGCAAGGt
gGCAATGACGggacaCCCCGTTCCa
    3F+[5]+3F
human, HBB G C ZP10766; ZP10767 (N/A) EL+KK, ELD+KKR OPEN / CoDA NHEJ; HR 21898685 R
ZN0146 aCCTGACTCCtgtggaGAAGTCTGCc
tGGACTGAGGacacctCTTCAGACGg
    3F+[6]+3F
human, HBB G C ZP10070, ZP10771 (N/A) EL+KK, ELD+KKR OPEN / CoDA NHEJ; HR 21898685 R
ZN0147 cCAGGCCCTCATTGTCacccagACCATGAAGGGCCTGg
gGTCCGGGAGTAACAGtgggtcTGGTACTTCCCGGACc
    5F+[6]+5F
cow, BLG G ZPU0033 ZPU0034 (N/A) (N/A) Sangamo NHEJ 21912434 R
ZN0147-T002 cCAGGCCCTCATCGTCacccagACCATGAAGGGCCTGg
gGTCCGGGAGTAGCAGtgggtcTGGTACTTCCCGGACc
    5F+[6]+5F
cow, BLG, exon with SNP G ZPU0033-T002 ZPU0034 (N/A) (N/A) Sangamo NHEJ 21912434 R Low
ZN0179 cCGCGTCCGCGCCatggcCATCTACAAGCAg
gGCGCAGGCGCGGtaccgGTAGATGTTCGTc
    4F+[5]+4F
human, TP53 G C ZP10845 ZP10846 TGEK Sharkey-AS+R MA(2F) NHEJ 21983922 R
ZN0187 tCAAGTCCAGTTCTACgggctcTCGGAGAATGACGAGTGGa
aGTTCAGGTCAAGATGcccgagAGCCTCTTACTGCTCACCt
    5F+[6]+6F
human, TRBC2/TRBC1 G C ZP10856 ZP10857 LRGS WT Sangamo NHEJ 22466705 R
ZN0188 gTGCTGTGGCCTGgAGCAACaaatctGACTTTGCATGTGCAa
cACGACACCGGACcTCGTTGtttagaCTGAAACGTACACGTt
    (2F+1+4F)+[6]+5F
human, TRAC G C ZP10858 ZP10859 LRGS WT Sangamo NHEJ 22466705 R
ZN0020B gGGCATCGACttcaagGAGGACGGCa
cCCGTAGCTGaagttcCTCCTGCCGt
    3F+[6]+3F
eGFP G ZPU0053 ZPU0054 TGGS Sharkey-AS+R PRS NHEJ 22640377 R
ZN0020B-T002 aTGCCTCGACatcgaaGAGGACGCCg
tACGGAGCTGtagcttCTCCTGCGGc
    3F+[6]+3F
sea squirt ZPU0053 ZPU0054 TGGS Sharkey-AS+R PRS NHEJ 22640377 R
ZN0020B-T003 aGAGATCGACtatgacGGGGACGGCg
tCTCTAGCTGatactgCCCCTGCCGc
    3F+[6]+3F
sea squirt ZPU0053 ZPU0054 TGGS Sharkey-AS+R PRS NHEJ 22640377 R
ZN0020C gGGCATCGACttcaagGAGGACGGCa
cCCGTAGCTGaagttcCTCCTGCCGt
    3F+[6]+3F
eGFP G ZP10885 ZP10884 TGGS Sharkey-AS+R PRS NHEJ 22642582 R
ZN0217 aTGCTGCCGGGGCCAGTTAccagctGATCCGtGGCCTGGGGc
tACGACGGCCCCGGTCAATggtcgaCTAGGCaCCGGACCCCg
    6F+[6]+(2F+1+3F)
rat, Prkdc G ZPU0073 ZPU0074 (N/A) (N/A) Sangamo NHEJ 22981234 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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