EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
All ZFN reported with no- or low-activity: 58 ZFN records match.
(Click on ID to see details)


ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0019-T003 aGTCATCCTCATCtcacgGATGAGGATGCCa
tCAGTAGGAGTAGagtgcCTACTCCTACGGt
    4F+[5]+4F
human, ABLIM2, intron G C ZP10064 ZP10064-T003 LRGS EL+KK Sangamo NHEJ 18587387 R Low
ZNA017B
(Z-M)
GTTGCGGGAt
CAACGCCCTa
    3F
(artificial) ZP00097 TGEK(G4S)3 Snase MA 18980382 R Low
ZNA017C
(Z-M)
GGTCGGGACc
CCAGCCCTGg
    3F
(artificial) ZP00125 TGEK(G4S)3 Snase MA 18980382 R Low
ZNA017D
(Z-M)
(target sequence error)
(please contact us)

    6F
(artificial) ZP00126 TGEK(G4S)3 Snase MA 18980382 R Low
ZN0050-T002 cCCCATCATCTATgccttCGTTGGGGAGAAGt
gGGGTAGTAGATAcggaaGCAACCCCTCTTCa
    4F+[5]+4F
human, CCR2 G C ZP10452 ZP10453-T002 TGEK WT, D+R, EL+KK MA NHEJ 19470664 R Low
ZN0061-T002 tAGCTCACaGTCCCTTGT AAAAGGGCCCaGGGAGCAg
aTCGAGTGtCAGGGAACA TTTTCCCGGGtCCCTCGTc
    5F+5F
human, PKP1, intron G C ZP10477-T002 ZP10477-T003 LRGS EL+KK Sangamo NHEJ 19680244 R Low
ZN0017-T002 aTCCCACCCGagtcctGCAGGTGTGt
tAGGGTGGGCtcaggaCGTCCACACa
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10047-T002 ZP10046-T002 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T003 aCACACCATCctacctTTGGTGGGTa
tGTGTGGTAGgatggaAACCACCCAt
    3F+[6]+3F
zebrafish, -, intron G C ZP10046-T003 ZP10047-T003 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T004 aCACACCTTCacagacGTGGGGGGAa
tGTGTGGAAGtgtctgCACCCCCCTt
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10046 ZP10047-T004 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T005 tCCCGACCAGattgtGAAGGTGTGt
aGGGCTGGTCtaacaCTTCCACACa
    3F+[5]+3F
zebrafish, fhdc1 G C ZP10047-T005 ZP10046 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T006 aACCCACCGAgatacGCGGGTGTGt
tTGGGTGGCTctatgCGCCCACACa
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10047-T006 ZP10046-T004 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T007 aCCCACCCTCgtgatgTTGGAGGGAa
tGGGTGGGAGcactacAACCTCCCTt
    3F+[6]+3F
zebrafish, odf3b G C ZP10046-T005 ZP10047-T007 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T008 cCACACCGGCagactGCGGCGGGAt
gGTGTGGCCGtctgaCGCCGCCCTa
    3F+[5]+3F
zebrafish, tmpoa G C ZP10046-T006 ZP10047-T008 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T009 cCACACCCACaaaagATGGTGGGTc
gGTGTGGGTGttttcTACCACCCAg
    3F+[5]+3F
zebrafish, tanc2, intron G C ZP10046-T007 ZP10047-T009 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T010 cTCCCACCCAggaagtGATGGTGAGt
gAGGGTGGGTccttcaCTACCACTCa
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10047-T010 ZP10046-T008 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T011 aTCCCACCGGagcggtGATGGTGAAc
tAGGGTGGCCtcgccaCTACCACTTg
    3F+[6]+3F
zebrafish, - G C ZP10047-T011 ZP10046-T009 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T012 gTCCCGCCAAcaaatGACGGAGTGc
cAGGGCGGTTgtttaCTGCCTCACg
    3F+[5]+3F
zebrafish, -, intron G C ZP10047-T012 ZP10046-T010 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T013 gCGCACCGCCagacatATGGTGGGAt
cGCGTGGCGGtctgtaTACCACCCTa
    3F+[6]+3F
zebrafish, sox6, intron G C ZP10046-T011 ZP10047-T013 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T014 aTCCCCCCTGccatgaGGAGGTGTGt
tAGGGGGGACggtactCCTCCACACa
    3F+[6]+3F
zebrafish, -, intron G C ZP10047-T014 ZP10046-T012 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T015 cACCCACCCActactGAGGGTGAGc
gTGGGTGGGTgatgaCTCCCACTCg
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10047-T015 ZP10046-T013 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T016 tCACACCTCCaattaGAGGCGGGAt
aGTGTGGAGGttaatCTCCGCCCTa
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10046-T012 ZP10047-T016 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T017 gTCCCTCCCTaagggtGATGGGGTGt
cAGGGAGGGAttcccaCTACCCCACa
    3F+[6]+3F
zebrafish, adcyap1b, 3'-UTR G C ZP10047-T017 ZP10046-T014 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T018 gCACACCAGCtgcattTTGGTGGGTc
cGTGTGGTCGacgtaaAACCACCCAg
    3F+[6]+3F
zebrafish, - G C ZP10046-T015 ZP10047-T003 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T019 cTTCCACCAAgtatcaGAAGGTGTAc
gAAGGTGGTTcatagtCTTCCACATg
    3F+[6]+3F
zebrafish, ctnna2 G C ZP10047-T018 ZP10046-T016 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T020 cTCCCACCAGgatatccgggttacGCAGGTGTGg
gAGGGTGGTCctataggcccaatgCGTCCACACc
    3F+[14]+3F
zebrafish, myo5aa G C ZP10047-T019 ZP10046-T002 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZNA014-T002 cAGCATCCATgtaagtATGGATGCTg
gTCGTAGGTAcattcaTACCTACGAc
    3F+[6]+3F
(artificial) ZP00121-T002 ZP00121-T002 (N/A) WT MA 21029755 R Low
ZNA014-T004 cTGCATCCCTgtaagtAGGGATGCAg
gACGTAGGGAcattcaTCCCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T004 ZP00121-T004 (N/A) WT MA 21029755 R Low
ZN0120-T002 cCACCCACAGGTTttaaggGTAGAAGCAGCAa
gGTGGGTGTCCAAaattccCATCTTCGTCGTt
    4F+[6]+4F
human, ADAMTS17, intron G C ZP10715-T002 ZP10716-T002 LRGS EL+KK Sangamo NHEJ 21533216 R Low
ZN0120-T003 cCACCTACTTGTCttgtgtGTTGAAGCAGACa
gGTGGATGAACAGaacacaCAACTTCGTCTGt
    4F+[6]+4F
human, (intergenic) G C ZP10715-T003 ZP10716-T003 LRGS EL+KK Sangamo NHEJ 21533216 R Low
ZN0127 cAACCTCGCCtatgtgGGCGCCGTCc
gTTGGAGCGGatacacCCGCGGCAGg
    3F+[6]+3F
human, IDUA G C ZP10728 ZP10729 TGGS / LGGS / LRGS ELD+KKR CoDA HR 21663559 R Low
ZN0136-T002 aAGGCAGGTGCTGTGTaagtacACTAAGGATGTGg
tTCCGTCCACGACACAttcatgTGATTCCTACACc
    5F+[6]+4F
mouse, (intergenic) G ZP10751-T002 ZP10752-T002 LRGS WT Sangamo NHEJ 21706032 R Low
ZN0147-T002 cCAGGCCCTCATCGTCacccagACCATGAAGGGCCTGg
gGTCCGGGAGTAGCAGtgggtcTGGTACTTCCCGGACc
    5F+[6]+5F
cow, BLG, exon with SNP G ZPU0033-T002 ZPU0034 (N/A) (N/A) Sangamo NHEJ 21912434 R Low
ZN0147B gGCCCTCATTGTCACCcagaccATGAAGGGCCTGGATa
cCGGGAGTAACAGTGGgtctggTACTTCCCGGACCTAt
    5F+[6]+5F
cow, BLG G ZPU0035 ZPU0036 (N/A) (N/A) Sangamo NHEJ 21912434 R Low
ZN0150 aCTCATCCACcagctGGAGATGGCa
tGAGTAGGTGgtcgaCCTCTACCGt
    3F+[5]+3F
zebrafish, ago2 G C ZP10774 ZP10773 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0151 aATCCGTGACggcgcaGTAAGGGCCc
tTAGGCACTGccgcgtCATTCCCGGg
    3F+[6]+3F
zebrafish, arhgef1 G C ZP10775 ZP10776 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0153 cCTCCTCCCCagagccGTAGTCGTAc
gGAGGAGGGGtctcggCATCAGCATg
    3F+[6]+3F
zebrafish, chd5 G C ZP10780 ZP10781 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0154 aCCCCTGGTCcggaaGTGTGGGTGt
tGGGGACCAGgccttCACACCCACa
    3F+[5]+3F
zebrafish, col18a1 G C ZP10782 ZP10783 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0155 cCTCAACACCgtcccGCAACGGAGc
gGAGTTGTGGcagggCGTTGCCTCg
    3F+[5]+3F
zebrafish, cxcl12b G C ZP10785 ZP10784 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0156 aCTCTCCTTCtccccGAGGGGGTCc
tGAGAGGAAGaggggCTCCCCCAGg
    3F+[5]+3F
zebrafish, dab2 G C ZP10786 ZP10787 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0157 tTTCCTCCACaaagaGGCTGGGATg
aAAGGAGGTGtttctCCGACCCTAc
    3F+[5]+3F
zebrafish, dicer1 G C ZP10789 ZP10790 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0158 tGTCCTCCTCatggcgGTCGATGGTt
aCAGGAGGAGtaccgcCAGCTACCAa
    3F+[6]+3F
zebrafish, drosha G C ZP10791 ZP10792 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0159 cCACGCCCAAgagggGCGGCAGCGg
gGTGCGGGTTctcccCGCCGTCGCc
    3F+[5]+3F
zebrafish, efnb2a G C ZP10794 ZP10793 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0160 aCCCCATTCCccctcaGCAAGAGTGg
tGGGGTAAGGgggagtCGTTCTCACc
    3F+[6]+3F
zebrafish, fli1a G C ZP10798 ZP10797 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0163 cTCCCGCACCagtttgGCATTGGGGt
gAGGGCGTGGtcaaacCGTAACCCCa
    3F+[6]+3F
zebrafish, kif1b G C ZP10805 ZP10806 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0165 aCTCTCCCCAgggagCAGGCAGCAt
tGAGAGGGGTccctcGTCCGTCGTa
    3F+[5]+3F
zebrafish, klf2a G C ZP10809 ZP10808 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0166 aGCCCTCCAAtctgaaGGTGCAGGAg
tCGGGAGGTTagacttCCACGTCCTc
    3F+[6]+3F
zebrafish, micall2 G C ZP10811 ZP10810 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0167 cACCCTCCTCaggctGTAGAGGAAg
gTGGGAGGAGtccgaCATCTCCTTc
    3F+[5]+3F
zebrafish, nf1a G C ZP10813 ZP10814 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0169 cCGTTACCTGtgtgagGAGGCAGAAa
gGCAATGGACacactcCTCCGTCTTt
    3F+[6]+3F
zebrafish, nf1b G C ZP10818 ZP10817 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0171 aCACCTCATCtggacGTGGAGGAGt
tGTGGAGTAGacctgCACCTCCTCa
    3F+[5]+3F
zebrafish, ptpru G C ZP10825 ZP10824 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0172 cCTCCTCCTCttcctCGGGTGGTGg
gGAGGAGGAGaaggaGCCCACCACc
    3F+[5]+3F
zebrafish, sbno2 G C ZP10828 ZP10829 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0174 cCTCCGCCCCttatcaCAGGTGGAGg
gGAGGCGGGGaatagtGTCCACCTCc
    3F+[6]+3F
zebrafish, spon1b G C ZP10834 ZP10835 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0175 tTCCCACCACtcagcAAGTGGGACg
aAGGGTGGTGagtcgTTCACCCTGc
    3F+[5]+3F
zebrafish, tie1 G C ZP10837 ZP10836 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0176 gACCCTCGGCcttcagCAGGTCGTAc
cTGGGAGCCGgaagtcGTCCAGCATg
    3F+[6]+3F
zebrafish, tarbp2 G C ZP10838 ZP10839 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0177 cACACACCTGtgagtcCAGGAGGACg
gTGTGTGGACactcagGTCCTCCTGc
    3F+[6]+3F
zebrafish, yrk G C ZP10841 ZP10842 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0191 aAACCATACCgaccgtGGGGAACTGa
tTTGGTATGGctggcaCCCCTTGACt
    3F+[6]+3F
zebrafish, hey2 G C ZP10864 ZP10865 LRGS D+R 2F-MA NHEJ 22543349 R Low
ZN0192 tCGTGCCAGCtgctccGACTCGGCCt
aGCACGGTCGacgaggCTGAGCCGGa
    3F+[6]+3F
zebrafish, rock1 G C ZP10866 ZP10867 LRGS D+R 2F-MA NHEJ 22543349 R No
ZN0199 cATCCAATTCCTTgcttcaGCAGTGGAAGGTa
gTAGGTTAAGGAAcgaagtCGTCACCTTCCAt
    4F+[6]+4F
zebrafish, lepr, exon-intron G C ZP10880 ZP10881 LRGS D+R 2F-MA NHEJ 22543349 R Low
ZN0200 tTACCATGCCCCTctgtatCAGGAAAAGGTAa
aATGGTACGGGGAgacataGTCCTTTTCCATt
    4F+[6]+4F
zebrafish, zgc:56306 G C ZP10882 ZP10883 LRGS D+R 2F-MA NHEJ 22543349 R Low




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
GSearch EENdb with this gene symbol
RSearch EENdb with this reference

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