EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
All ZFNs with full sequence provided by original references: 76 ZFN records match.
(Click on ID to see details)


ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZNA005
(Z-M)
GGGGCGGAAn
CCCCGCCTTn
    3F
(artificial) ZP00009 QNKKGLD(G4S)3 WT 9862996 R
ZNA005-T002
(Z-M)
GGGGCAGAAn
CCCCGTCTTn
    3F
(artificial) ZP00009-T002 QNKKGLD(G4S)3 WT 9862996 R
ZNA005-T003
(Z-M)
GGGTCAGAAn
CCCAGTCTTn
    3F
(artificial) ZP00009-T003 QNKKGLD(G4S)3 WT 9862996 R
ZN0003 tCGGAGCCGCTTTaacccACTCTGTGGAAGt
aGCCTCGGCGAAAttgggTGAGACACCTTCa
    4F+[5]+4F
human, IL2RG G C ZP10005 ZP10006 (N/A) WT Sangamo HR 15806097 R
ZN0004 cTGACTCCTGatgttgattTTGGGAGAAc
gACTGAGGACtacaactaaAACCCTCTTg
    3F+[9]+3F
mouse, Cftr G ZP10008 ZP10007 TG(G4S)3 WT MA 16084494 R
ZN0005 gTTCCCCTTCaaagggGTGGATGACc
cAAGGGGAAGtttcccCACCTACTGg
    3F+[6]+3F
mouse, Tyr G ZP10010 ZP10009 TG WT MA 16084494 R
ZN0006 tGTCCCCTTCtgggctcactatGCTGCCGCCc
aCAGGGGAAGacccgagtgataCGACGGCGGg
    3F+[12]+3F
human, CCR5 G C ZP10013 ZP10014 TG(G4S)3 WT MA 16084494 R
ZN0007 gGCCCGCCCCctagcgGCCGGGGAGg
cCGGGCGGGGgatcgcCGGCCCCTCc
    3F+[6]+3F
human, DMPK, 3'-UTR G C ZP10012 ZP10011 TG WT MA 16084494 R
ZN0014 gGCCGCACGCTGCacagatGCACAGCAGGTTa
cCGGCGTGCGACGtgtctaCGTGTCGTCCAAt
    4F+[6]+4F
zebrafish, slc24a5 G C ZP10039 ZP10038 LRGS EL+KK Sangamo NHEJ 18500334 R
ZN0015 gTTCATCCAGACGgtgtttGTGGACGTGGGCt
cAAGTAGGTCTGCcacaaaCACCTGCACCCGa
    4F+[6]+4F
zebrafish, slc24a5 G C ZP10041 ZP10040, ZP10042 LRGS EL+KK Sangamo NHEJ 18500334 R
ZN0016 tCTCGACCCTAATgcaatgTACTCGGTCCTGc
aGAGCTGGGATTAcgttacATGAGCCAGGACg
    4F+[6]+4F
zebrafish, ntla G C ZP10043 ZP10044, ZP10045 LRGS WT Sangamo NHEJ 18500334 R
ZN0019 gGTCATCCTCATCctgatAAACTGCAAAAGg
cCAGTAGGAGTAGgactaTTTGACGTTTTCc
    4F+[5]+4F
human, CCR5 G C ZP10064 ZP10065 LRGS WT, EL+KK Sangamo NHEJ 18587387 R
ZN0019-T002 gGTCGTCCTCATCttaatAAACTGCAAAAAg
cCAGCAGGAGTAGaattaTTTGACGTTTTTc
    4F+[5]+4F
human, CCR2 G C ZP10064-T002 ZP10065-T002 LRGS EL+KK Sangamo NHEJ 18587387 R
ZN0019-T003 aGTCATCCTCATCtcacgGATGAGGATGCCa
tCAGTAGGAGTAGagtgcCTACTCCTACGGt
    4F+[5]+4F
human, ABLIM2, intron G C ZP10064 ZP10064-T003 LRGS EL+KK Sangamo NHEJ 18587387 R Low
ZN0002B cTACCCCGACcacatGAAGCAGCAc
gATGGGGCTGgtgtaCTTCGTCGTg
    3F+[5]+3F
eGFP G ZP10066, ZP10070 ZP10073, ZP10077 LRGS WT OPEN NHEJ 18657511 R
ZN0008D cACCATCTTCttcaagGACGACGGCa
gTGGTAGAAGaagttcCTGCTGCCGt
    3F+[6]+3F
eGFP G ZP10089, ZP10092 ZP10096, ZP10099 LRGS WT OPEN NHEJ 18657511 R
ZN0020 gGGCATCGACttcaagGAGGACGGCa
cCCGTAGCTGaagttcCTCCTGCCGt
    3F+[6]+3F
eGFP G ZP10104, ZP10105 ZP10111, ZP10112 LRGS WT OPEN NHEJ 18657511 R
ZN0021 gATCCGCCACaacatcGAGGACGGCa
cTAGGCGGTGttgtagCTCCTGCCGt
    3F+[6]+3F
eGFP G ZP10120, ZP10121 ZP10128, ZP10134 LRGS WT OPEN NHEJ 18657511 R
ZN0022 tCACTTCTAAtggtgaTTATGGGAGa
aGTGAAGATTaccactAATACCCTCt
    3F+[6]+3F
human, CFTR, intron G C ZP10153 ZP10165 LRGS WT OPEN NHEJ 18657511 R
ZN0023 cAGCGTCTTCgagagtGAGGACGTGt
gTCGCAGAAGctctcaCTCCTGCACa
    3F+[6]+3F
human, VEGFA, 5'-up G C ZP10176, ZP10181 ZP10186, ZP10190 LRGS WT, EL+KK OPEN NHEJ; HR 18657511 R
ZN0024 gAGCAGCGTCttcgaGAGTGAGGAc
cTCGTCGCAGaagctCTCACTCCTg
    3F+[5]+3F
human, VEGFA, 5'-up G C ZP10213, ZP10216 ZP10224, ZP10227 LRGS WT, EL+KK OPEN NHEJ; HR 18657511 R
ZN0025 cCCCAGCCGCcccactGAGTTTGCCt
gGGGTCGGCGgggtgaCTCAAACGGa
    3F+[6]+3F
human, HOXB13 G C ZP10280, ZP10282 ZP10284, ZP10285 LRGS WT OPEN NHEJ 18657511 R
ZN0026 aTGCCACCCAtgcccTGGGGTGCCc
tACGGTGGGTacgggACCCCACGGg
    3F+[5]+3F
human, HOXB13 G C ZP10297, ZP10299 ZP10302, ZP10306 LRGS WT OPEN NHEJ 18657511 R
ZN0027 gAGACTCCCAcggccGGGGAAGAGt
cTCTGAGGGTgccggCCCCTTCTCa
    3F+[5]+3F
human, HOXB13 G C ZP10314, ZP10319 ZP10325, ZP10326 LRGS WT OPEN NHEJ 18657511 R
ZN0028 gGAATCCTTCtaatgaGGCGGAGATc
cCTTAGGAAGattactCCGCCTCTAg
    3F+[6]+3F
tobacco, SuRB/SuRA G ZP10335 ZP10337 LRGS WT OPEN NHEJ 18657511 R
ZN0039 cCTGTGGGGCCATatcccaGAACTGGTTGAGTCGGTCa
gGACACCCCGGTAtagggtCTTGACCAACTCAGCCAGt
    4F+[6]+6F
maize, ipp2K G ZP10429 ZP10430, ZP10431 LRGS EL+KK Sangamo NHEJ; HR 19404259 R
ZN0039-T002 cCTGTGGGGCCATattccaGAACTGGTTGAGTCTGTCa
gGACACCCCGGTAtaaggtCTTGACCAACTCAGACAGt
    4F+[6]+6F
maize, LOC100126363 G ZP10429 ZP10431-T002 LRGS EL+KK Sangamo NHEJ; HR 19404259 R
ZN0040 cCAACACCTGGGTGCCaatcatGTCGATGGTGGGGTATGGt
gGTTGTGGACCCACGGttagtaCAGCTACCACCCCATACCa
    5F+[6]+6F
maize, ipp2K G ZP10432; ZPU0001 ZP10433; ZPU0002 LRGS EL+KK Sangamo NHEJ; HR 19404259 R
ZN0041 aCCCCTACGCGGAgtacctGAGGCAGCCGCAg
tGGGGATGCGCCTcatggaCTCCGTCGGCGTc
    4F+[6]+4F
maize, Zp15 G ZP10434 ZP10435 LRGS EL+KK Sangamo NHEJ; HR 19404259 R
ZN0042 cGCCCTACTACGCcgggtgTGGGCAGCCGAGc
gCGGGATGATGCGgcccacACCCGTCGGCTCg
    4F+[6]+4F
maize, Zp15 G ZP10436 ZP10437 LRGS EL+KK Sangamo NHEJ; HR 19404259 R
ZN0043 aAGTCCAATCTATgacatcAATTATTATACAt
tTCAGGTTAGATActgtagTTAATAATATGTa
    4F+[6]+4F
human, CCR5 G C ZP10438 ZP10439 TGEK WT MA NHEJ 19470664 R
ZN0044 tCTGGGCTCACTAtgctgcCGCCCAGTGGGAc
aGACCCGAGTGATacgacgGCGGGTCACCCTg
    4F+[6]+4F
human, CCR5 G C ZP10440 ZP10441 TGEK WT MA NHEJ 19470664 R
ZN0045 cTTCTTCATCATCctcctGACAATCGAt
gAAGAAGTAGTAGgaggaCTGTTAGCTa
    4F+[5]+3F
human, CCR5/CCR2 G C ZP10442 ZP10443 TGEK WT MA NHEJ 19470664 R
ZN0046 cTGTGTTTGCTTTaaaagcCAGGACGGTCACc
gACACAAACGAAAttttcgGTCCTGCCAGTGg
    4F+[6]+4F
human, CCR5/CCR2 G C ZP10444 ZP10445 TGEK WT MA NHEJ 19470664 R
ZN0047 cAGGACGGTCacctttGGGGTGGTGa
gTCCTGCCAGtggaaaCCCCACCACt
    3F+[6]+3F
human, CCR5 G C ZP10446 ZP10447 TGEK WT MA NHEJ 19470664 R
ZN0048 aCGGTCACCTttggggTGGTGACAAGTGt
tGCCAGTGGAaaccccACCACTGTTCACa
    3F+[6]+4F
human, CCR5/CCR2 G C ZP10448 ZP10449 TGEK WT MA NHEJ 19470664 R
ZN0049 aCCAAGCTATgcaggtGACAGAGACt
tGGTTCGATAcgtccaCTGTCTCTGa
    3F+[6]+3F
human, CCR5 G C ZP10450 ZP10451 TGEK WT MA NHEJ 19470664 R
ZN0050 cCCCATCATCTATgcctttGTCGGGGAGAAGt
gGGGTAGTAGATAcggaaaCAGCCCCTCTTCa
    4F+[6]+4F
human, CCR5 G C ZP10452 ZP10453 TGEK WT, D+R, EL+KK MA NHEJ 19470664 R
ZN0050-T002 cCCCATCATCTATgccttCGTTGGGGAGAAGt
gGGGTAGTAGATAcggaaGCAACCCCTCTTCa
    4F+[5]+4F
human, CCR2 G C ZP10452 ZP10453-T002 TGEK WT, D+R, EL+KK MA NHEJ 19470664 R Low
ZN0051 aGGCTACATCtttgcTTTGTTGGCa
tCCGATGTAGaaacgAAACAACCGt
    3F+[5]+3F
human, PIGA G C ZP10454; ZP10455 ZP10457; ZP10456 LRGS WT OPEN NHEJ; HR 19540188 R
ZN0057 cACCCCACAGTGGggccacTAGGGACAGGATt
gTGGGGTGTCACCccggtgATCCCTGTCCTAa
    4F+[6]+4F
human, PPP1R12C, intron G C ZP10468 ZP10469 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0058 cCAGACCtGGCACCCAGgagagGAGCAGGCAGGGTCAGCTg
gGTCTGGaCCGTGGGTCctctcCTCGTCCGTCCCAGTCGAc
    (3F+1+2F)+[5]+6F
human, POU5F1, intron G C ZP10470 ZP10471 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0059 cTGCAGCTGCCCAGACCTGgcaccCAGGAGaGGAGCAGGCa
gACGTCGACGGGTCTGGACcgtggGTCCTCtCCTCGTCCGt
    6F+[5]+(2F+1+3F)
human, POU5F1, intron G C ZP10472 ZP10473 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0060 aGTAGTCCTTTGTTACatgcatGAGTCAGTGAACAGGg
tCATCAGGAAACAATGtacgtaCTCAGTCACTTGTCCc
    5F+[6]+5F
human, POU5F1, intron G C ZP10474 ZP10475 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0061 cCCCAGCTCCCAGAGCacggctGCAAGGGCCaGGAGCAc
gGGGTCGAGGGTCTCGtgccgaCGTTCCCGGtCCTCGTg
    5F+[6]+(3F+1+2F)
human, PITX3 G C ZP10477 ZP10476 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0061-T002 tAGCTCACaGTCCCTTGT AAAAGGGCCCaGGGAGCAg
aTCGAGTGtCAGGGAACA TTTTCCCGGGtCCCTCGTc
    5F+5F
human, PKP1, intron G C ZP10477-T002 ZP10477-T003 LRGS EL+KK Sangamo NHEJ 19680244 R Low
ZN0087 cGCGATCACCaagtacGCAGATCTAa
gCGCTAGTGGttcatgCGTCTAGATt
    3F+[6]+3F
silk worm, BmBLOS2 G ZP10547 ZP10546 TGQK WT MA NHEJ 20692340 R
ZN0088 gAGTCTCAGCacagaaGACATAGAAa
cTCAGAGTCGtgtcttCTGTATCTTt
    3F+[6]+3F
silk worm, BmBLOS2 G ZP10549 ZP10548 TGQK WT MA NHEJ 20692340 R
ZN0089 aGTTACTTACacgtggGCCGATGTCa
tCAATGAATGtgcaccCGGCTACAGt
    3F+[6]+3F
silk worm, Bmwh3 G ZP10551 ZP10550 TGQK WT MA NHEJ 20692340 R
ZN0092 tCAAATCTGCCGTGTAtgtgggGACAAGGCCaATGGCTa
aGTTTAGACGGCACATacacccCTGTTCCGGtTACCGAt
    5F+[6]+(3F+1+2F)
rat, Nr1i2 G ZP10555 ZP10556 LRGS WT Sangamo NHEJ; HR 21151125 R
ZN0116 cCCTCAAgCAGGCCCCGCTGgtgcactGAAGAGcCACCCTGTGg
gGGAGTTcGTCCGGGGCGACcacgtgaCTTCTCgGTGGGACACc
    (4F+1+2F)+[7]+(2F+1+3F)
human, CLTA G C ZP10703 ZP10704 LRGS ELD+KKR Sangamo HR 21297641 R
ZN0117 gGGGGGCCTCaCGCACCcgcggcGCAGGAGCTTCAGTGg
cCCCCCGGAGtGCGTGGgcgccgCGTCCTCGAAGTCACc
    (2F+1+3F)+[6]+5F
human, DNM2 G C ZP10705 ZP10706 LRGS ELD+KKR Sangamo HR 21297641 R
ZN0116B cCCTCAAgCAGGCCCCGCTGgtgcactGAAGAGcCACCCTGTGg
gGGAGTTcGTCCGGGGCGACcacgtgaCTTCTCgGTGGGACACc
    (4F+1+2F)+[7]+(2F+1+3F)
monkey, CLTA G ZP10703 ZP10704 LRGS ELD+KKR Sangamo HR 21297641 R
ZN0118 cCTTATTgAGCATCCGGATCctatcTATGATCCCAAGGAGAAGg
gGAATAAcTCGTAGGCCTAGgatagATACTAGGGTTCCTCTTCc
    (4F+1+2F)+[5]+6F
frog, nog G ZP10713, ZP10714 ZP10711, ZP10712 LRGS EL+KK Sangamo NHEJ 21471457 R
ZN0122 gGACTCCTTCaccaaggGGAGGGGGAg
cCTGAGGAAGtggttccCCTCCCCCTc
    3F+[7]+3F
human, IDUA, intron-exon G C ZP10718 ZP10719 TGPNRGVTK ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0123 gGCCCCCCCCcgccccGCAGATGAGg
cCGGGGGGGGgcggggCGTCTACTCc
    3F+[6]+3F
human, IDUA, intron-exon G C ZP10720 ZP10721 TGGS / LGGS / LRGS ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0124 aCCCTGCGCCccgcgctGCGGCTGCCg
tGGGACGCGGggcgcgaCGCCGACGGc
    3F+[7]+3F
human, IDUA G C ZP10722 ZP10723 TGPNRGVTK ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0125 gCCCCGCCCCttacccGCCGGCGGCc
cGGGGCGGGGaatgggCGGCCGCCGg
    3F+[6]+3F
human, IDUA G C ZP10724 ZP10725 TGGS / LGGS / LRGS ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0126 gCTCAACCTCgcctatGTGGGCGCCg
cGAGTTGGAGcggataCACCCGCGGc
    3F+[6]+3F
human, IDUA G C ZP10726 ZP10727 TGGS / LGGS / LRGS ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0127 cAACCTCGCCtatgtgGGCGCCGTCc
gTTGGAGCGGatacacCCGCGGCAGg
    3F+[6]+3F
human, IDUA G C ZP10728 ZP10729 TGGS / LGGS / LRGS ELD+KKR CoDA HR 21663559 R Low
ZN0130 tCCCCTGCTTGGCtgggcgCAGTGGCTCATGc
aGGGGACGAACCGacccgcGTCACCGAGTACg
    4F+[6]+4F
human, TP53, intron G C ZP10734 ZP10735 QNKK WT, EL+KK MMA NHEJ; HR 21695267 R
ZN0131 cCACCATCCACTAcaactaCATGTGTAACAGt
gGTGGTAGGTGATgttgatGTACACATTGTCa
    4F+[6]+4F
human, TP53 G C ZP10736 ZP10737 QNKK WT, EL+KK MMA NHEJ; HR 21695267 R
ZN0136 gTGCCAGGTACTGTGTcagggtACTAGGGGTATGg
cACGGTCCATGACACAgtcccaTGATCCCCATACc
    5F+[6]+4F
human, F9, intron G C ZP10751 ZP10752 LRGS WT Sangamo NHEJ; HR 21706032 R
ZN0136-T002 aAGGCAGGTGCTGTGTaagtacACTAAGGATGTGg
tTCCGTCCACGACACAttcatgTGATTCCTACACc
    5F+[6]+4F
mouse, (intergenic) G ZP10751-T002 ZP10752-T002 LRGS WT Sangamo NHEJ 21706032 R Low
ZN0138 gGGAGTGgTGCATGGTGtggcaaCAGGTAaGCTCCATTGTGCtt
cCCTCACcACGTACCACaccgttGTCCATtCGAGGTAACACGaa
    (3F+1+2F)+[6]+(2F+1+4F)
human, SNCA G C ZP10755, ZP10756 ZP10757, ZP10758; ZP10759 LRGS WT, ELD+KKR Sangamo NHEJ; HR 21757228 R
ZN0141 cCACATCCCAacagaGCAGCGGGAg
gGTGTAGGGTtgtctCGTCGCCCTc
    3F+[5]+3F
zebrafish, hey2 G C ZP10760 ZP10761 LRGS EL+KK (N/A) NHEJ 21822241 R
ZN0142 tAGCTTCTCAGTCacgcacGCCTGTGAGTTTc
aTCGAAGAGTCAGtgcgtgCGGACACTCAAAg
    4F+[6]+4F
zebrafish, gria3a G C ZP10763 ZP10762 LRGS EL+KK (N/A) NHEJ 21822241 R
ZN0143 aTACGCCtCGCCCGAGCtgtgcgGTAGGCATTGAGGCAGCCa
tATGCGGaGCGGGCTCGacacgcCATCCGTAACTCCGTCGGt
    (3F+1+2F)+[6]+6F
human, MALAT1, promoter G C ZP10764 ZP10765 LRGS EL+KK Sangamo NHEJ; HR 21844124 R
ZN0179 cCGCGTCCGCGCCatggcCATCTACAAGCAg
gGCGCAGGCGCGGtaccgGTAGATGTTCGTc
    4F+[5]+4F
human, TP53 G C ZP10845 ZP10846 TGEK Sharkey-AS+R MA(2F) NHEJ 21983922 R
ZN0181 gTGCTGACCATCGacaagaAAGGGActGAAGCTGCTg
cACGACTGGTAGCtgttctTTCCCTgaCTTCGACGAc
    4F+[6]+(2F+2+3F)
human, A1AT G C ZP10847 ZP10848 LRGS WT Sangamo NHEJ; HR 21993621 R
ZN0086B cTGCAACTCCAGTCTTTCTagaagaTGGGCGGGAGTCt
gACGTTGAGGTCAGAAAGAtcttctACCCGCCCTCAGa
    6F+[6]+4F
mouse, G ZP10852 ZP10853 TGAAARA(-Q) WT, Sharkey-ELD+KKR MA NHEJ; HR 22169954 R
ZN0020B gGGCATCGACttcaagGAGGACGGCa
cCCGTAGCTGaagttcCTCCTGCCGt
    3F+[6]+3F
eGFP G ZPU0053 ZPU0054 TGGS Sharkey-AS+R PRS NHEJ 22640377 R
ZN0214 tGTCTGCTCCtcagctGGTGATGCTg
aCAGACGAGGagtcgaCCACTACGAc
    3F+[6]+3F
zebrafish, rest G C ZP10901, ZP10902 ZP10903 (N/A) (N/A) (N/A) NHEJ 22951640 R
ZN0215 cAGCTACTCCgacagcGAAGAAGACg
gTCGATGAGGctgtcgCTTCTTCTGc
    3F+[6]+3F
zebrafish, rest G C ZP10905, ZP10906 ZP10907 (N/A) (N/A) (N/A) NHEJ 22951640 R
ZN0216 gCGCCCCTGCgcaacGTGGCAGGAa
cGCGGGGACGcgttgCACCGTCCTt
    3F+[5]+3F
mouse, G ZP10916 ZP10915 LRGS WT, EL+KK OPEN NHEJ; HR 22970113 R
ZN0219 cACCTCCTCCccggccGGCGGCGGAc
gTGGAGGAGGggccggCCGCCGCCTg
    3F+[6]+3F
human, VEGFA G C ZP10917 - ZP10920 ZP10920 - ZP10923 LRGS D+R OPEN HR 23028823 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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