EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN first reported by the reference 22655075: 5 ZFN, 6 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0146 tGCTCAAACCCGACGAGCatcaccacatcgaggacgAGCACGTGAGGGCGCCCa
aCGAGTTTGGGCTGCTCGtagtggtgtagctcctgcTCGTGCACTCCCGCGGGt
    17+[18]+17
zebrafish, myod1 G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0147 tATTTCTGCGCAGAAGTGgccccaccaagcctgctGGAGGACATACAGAATTa
aTAAAGACGCGTCTTCACcggggtggttcggacgaCCTCCTGTATGTCTTAAt
    17+[17]+17
zebrafish, jak3 G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0145 tCAGCCTTGGTCACATCTcattcagacagtgaaaACATCGGAGGCTTTTCTa
aGTCGGAACCAGTGTAGAgtaagtctgtcactttTGTAGCCTCCGAAAAGAt
    17+[16]+17
zebrafish, phf6 G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0144 tGGAGTGTATGAAAACTGgtcatgtcaggacagaGAAAAGGAGCTTCAGCa
aCCTCACATACTTTTGACcagtacagtcctgtctCTTTTCCTCGAAGTCGt
    17+[16]+16
zebrafish, phf6, ATG G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0142 tTCTTTTCTATCCCATGAcgatgcatcgcacaacAAGGATCAAGATTACAGa
aAGAAAAGATAGGGTACTgctacgtagcgtgttgTTCCTAGTTCTAATGTCt
    17+[16]+17
zebrafish, bmi1a, ATG G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0143 tACCTGCACAGGATGCAgactgaggaggcacagGAAATGTCCCAGATAACa
aTGGACGTGTCCTACGTctgactcctccgtgtcCTTTACAGGGTCTATTGt
    16+[16]+17
zebrafish, ikzf1, ATG G C (std.) +63 WT FLASH NHEJ 22655075 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0202 gAGCATCACCacatcGAGGACGAGc
cTCGTAGTGGtgtagCTCCTGCTCg
    3F+[5]+3F
zebrafish, myod1 G C ZP10886 ZP10887 (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZNU012 (N/A)
    
zebrafish, cdh15 G C (N/A) (N/A) (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZNU013 (N/A)
    
zebrafish, yap G C (N/A) (N/A) (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZNU014 (N/A)
    
zebrafish, ptenb G C (N/A) (N/A) (N/A) EL+KK CoDA NHEJ 22655075 R
ZNU015 (N/A)
    
zebrafish, tp53 G C (N/A) (N/A) (N/A) ELD+KKR CoDA NHEJ 22655075 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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