EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN first reported by the reference 21700836: 4 ZFN, 4 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0024 tgggatCCAGCCTGATGGAACt...gATTTGCAGTTGGAAAGAatcaa
accctaGGTCGGACTACCTTGa...cTAAACGTCAACCTTTCTtagtt
    15+[17]+17
worm, ben-1 G (std.) +63 WT (N/A) NHEJ 21700836 R
TN0024B tgggatCCAGCCTGATGGAACt...tGCAGTTGGAAAGAATCAa
accctaGGTCGGACTACCTTGa...aCGTCAACCTTTCTTAGTt
    15+[21]+17
worm, ben-1 G (std.) +63 WT (N/A) NHEJ 21700836 R No
TN0024C tGGGATCCAGCCTGATg...gATTTGCAGTTGGAAAGAatcaa
aCCCTAGGTCGGACTAc...cTAAACGTCAACCTTTCTtagtt
    15+[22]+17
worm, ben-1 G (std.) +63 WT (N/A) NHEJ 21700836 R No
TN0024D tgggatCCAGCCTGATGGAACTTa...tGCAGTTGGAAAGAATCAa
accctaGGTCGGACTACCTTGAAt...aCGTCAACCTTTCTTAGTt
    17+[19]+17
worm, ben-1 G (std.) +63 WT (N/A) (Inactive) 21700836 R No



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0132 aGTTGTCCCAATTCTTGTTgaattaGATGGTGATgtTAATGGg
tCAACAGGGTTAAGAACAActtaatCTACCACTAcaATTACCc
    6F+[6]+(3F+2+2F)
eGFP G ZP10740 ZP10741, ZP10742 (N/A) WT Sangamo NHEJ 21700836 R
ZN0133 cAGCCTGATGgAACTTAtaagggAGAAAGtGATTTGCAGt
gTCGGACTACcTTGAATattcccTCTTTCaCTAAACGTCa
    (2F+1+3F)+[6]+(2F+1+3F)
worm, ben-1 G ZP10743 ZP10744 (N/A) WT, ELD+KKR(?) Sangamo NHEJ 21700836 R
ZN0134 gCAGCAGTGCGTGCGTACAaaaggaGACAAGcGCAGGGaGAACTGg
cGTCGTCACGCACGCATGTtttcctCTGTTCgCGTCCCtCTTGACc
    6F+[6]+(2F+1+2F+1+2F)
worm, rex-1 [cis-element] G ZP10745 ZP10746 (N/A) WT Sangamo NHEJ 21700836 R
ZN0135 cCCGAGCtCACATCGATgatcaaGAAAACACGGCAGCAa
gGGCTCGaGTGTAGCTActagttCTTTTGTGCCGTCGTt
    (3F+1+2F)+[6]+5F
worm, Cbr-sdc-2 G ZP10749 ZP10750 (N/A) WT Sangamo NHEJ 21700836 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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