EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN first reported by the reference 21493687: 0 ZFN, 30 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0009 tGCATACCTAATCATTATgctgaggatttggaaAGGGTGTTTATTCCTCATGGa
aCGTATGGATTAGTAATAcgactcctaaaccttTCCCACAAATAAGGAGTACCt
    17+[15]+20
human, HPRT1 G C (std.) +231 WT GG(V&B) 21493687 R
TN0010 tCCATTCCTATGACTGTAGATtttatcagactgaagAGCTATTGTGTGAGTATa
aGGTAAGGATACTGACATCTAaaatagtctgacttcTCGATAACACACTCATAt
    20+[15]+17
human, HPRT1 G C (std.) +231 WT GG(V&B) NHEJ, HR 21493687 R
TN0011 tGGATTATGCCTGGCACCATTAAAGAAAATa...tATAGATACAGAAGCGTCATCAAAGCATGCCa
aCCTAATACGGACCGTGGTAATTTCTTTTAt...aTATCTATGTCTTCGCAGTAGTTTCGTACGGt
    29+[28]+30
human, CFTR G C (others) +231 WT GG(V&B) 21493687 R
TN0012 tGGCACCATTAAAGAAAATATCATc...tAGATACAGAAGCGTCATCAAAGCATGCCa
aCCGTGGTAATTTCTTTTATAGTAg...aTCTATGTCTTCGCAGTAGTTTCGTACGGt
    23+[25]+28
human, CFTR G C (std.) +231 WT GG(V&B) (Weak) 21493687 R Low
TN0013 tATTTCGACGCTCATTCTctggccatggacttcttgAGCATCGGCTTCCGGGa
aTAAAGCTGCGAGTAAGAgaccggtacctgaagaacTCGTAGCCGAAGGCCCt
    17+[18]+16
zebrafish, hey2 G C (std.) +231 WT GG(V&B) 21493687 R
TN0014 tCCTGACCATTCATCAGCCGTCTt...cAAGATCCTTCTGATGGCCGAGGGCa
aGGACTGGTAAGTAGTCGGCAGAa...gTTCTAGGAAGACTACCGGCTCCCGt
    22+[24]+24
fruitfly, w G (others) +231 WT GG(V&B) 21493687 R
TN0015 tCCATGGCCAACACTTGTCACTACTTTc...aAGATACCCAGATCATATGAAGCGGCa
aGGTACCGGTTGTGAACAGTGATGAAAg...tTCTATGGGTCTAGTATACTTCGCCGt
    26+[25]+25
GFP G (std.) +231 WT GG(V&B) 21493687 R
TN0016 tACAACTACAACAGCCACAACGTCTa...aAGAACGGCATCAAGGTGAACTTCAAGa
aTGTTGATGTTGTCGGTGTTGCAGAt...tTCTTGCCGTAGTTCCACTTGAAGTTCt
    24+[21]+27
eGFP G (std.) +231 WT GG(V&B) 21493687 R
TN0017 tCGCAAAGAATTTCTTTAATt...cATCTTCGTTAAGTTTCCCGTGTa
aGCGTTTCTTAAAGAAATTAa...gTAGAAGCAATTCAAAGGGCACAt
    19+[15]+22
Plasmodium, PMV G (std.) +231 WT GG(V&B) 21493687 R
TN0018 tATTATTGGCCTTAACCATACTCTt...tATGTCAGAAAAATGTTTTACATGGa
aTAATAACCGGAATTGGTATGAGAa...aTACAGTCTTTTTACAAAATGTACCt
    23+[15]+24
Plasmodium, PMV G (std.) +231 WT GG(V&B) 21493687 R
TN0019 tCCATCTCCTCAAGGATGTTc...cAAGAACATTGTGAAGAGTCTAGa
aGGTAGAGGAGTTCCTACAAg...gTTCTTGTAACACTTCTCAGATCt
    19+[15]+22
thale cress, TT4 G (std.) +231 WT GG(V&B) 21493687 R
TN0020 tGTCGTCTTCTGCACTacctccggcgtcgacATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAtggaggccgcagctgTACGGACCACGACTGAt
    15+[15]+16
thale cress, TT4 G (std.) +231 WT GG(V&B) 21493687 R
TN0021 tCCGGATGCTCCTCTTgacaaggtctgtattgtcAGTTGTGGTTTGTCTa
aGGCCTACGAGGAGAActgttccagacataacagTCAACACCAAACAGAt
    15+[18]+15
thale cress, ADH1 G (std.) +231 WT GG(V&B) NHEJ, HR 21493687 R
TN0022 tACTGAATAATCAACACTtgggaatggtggttcAATGGGAGGATCGGTTCTATa
aTGACTTATTAGTTGTGAacccttaccaccaagTTACCCTCCTAGCCAAGATAt
    17+[15]+20
tobacco, SuRB G (std.) +231 WT GG(V&B) 21493687 R
TN0023 tACCTCATCAGGAACATGTTctacctatgattcccAGTGGCGGGGCTTTCAAAGa
aTGGAGTAGTCCTTGTACAAgatggatactaagggTCACCGCCCCGAAAGTTTCt
    19+[15]+19
tobacco, SuRB G (std.) +231 WT GG(V&B) 21493687 R
TNA002B tCCCAGAAGTAAAn(x)TTTACTTCTGGGa
aGGGTCTTCATTTn(x)AAATGAAGACCCt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA004B tAGACAAACCACAn(x)TGTGGTTTGTCTa
aTCTGTTTGGTGTn(x)ACACCAAACAGAt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA021 tGGCACTGCTAACCCTn(x)AGGGTTAGCAGTGCCa
aCCGTGACGATTGGGAn(x)TCCCAATCGTCACGGt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA022 tCGGTTTGCTATCGTTn(x)AACGATAGCAAACCGa
aGCCAAACGATAGCAAn(x)TTGCTATCGTTTGGCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA023 tCCCAGAAGTAAACATn(x)ATGTTTACTTCTGGGa
aGGGTCTTCATTTGTAn(x)TACAAATGAAGACCCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA003B tCCGGATGCTCCTn(x)AGGAGCATCCGGa
aGGCCTACGAGGAn(x)TCCTCGTAGGCCt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA024 tACCCCTCTCCn(x)GGAGAGGGGTa
aTGGGGAGAGGn(x)CCTCTCCCCAt
    10+[x]+10
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA025 tCTTGTCCGCATGATTn(x)AATCATGCGGACAAGa
aGAACAGGCGTACTAAn(x)TTAGTACGCCTGTTCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA026 tATTCCCCACGAGCTCTn(x)AGAGCTCGTGGGGAATa
aTAAGGGGTGCTCGAGAn(x)TCTCGAGCACCCCTTAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA027 tAGACAGGAAGTGGAGCn(x)GCTCCACTTCCTGTCTa
aTCTGTCCTTCACCTCGn(x)CGAGGTGAAGGACAGAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA001B tATCAAGATTCTCn(x)GAGAATCTTGATa
aTAGTTCTAAGAGn(x)CTCTTAGAACTAt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA028 tAGACAAACCACAACTn(x)AGTTGTGGTTTGTCTa
aTCTGTTTGGTGTTGAn(x)TCAACACCAAACAGAt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA005B tACCCCTCTCCGCTn(x)AGCGGAGAGGGGTa
aTGGGGAGAGGCGAn(x)TCGCCTCTCCCCAt
    13+[x]+13
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA029 tCCGGATGCTCCTCTTn(x)AAGAGGAGCATCCGGa
aGGCCTACGAGGAGAAn(x)TTCTCCTCGTAGGCCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA030 tACCCCTCTCCGCTTCTn(x)AGAAGCGGAGAGGGGTa
aTGGGGAGAGGCGAAGAn(x)TCTTCGCCTCTCCCCAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 21493687 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
GSearch EENdb with this gene symbol
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