EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN first reported by the reference 21459844: 0 ZFN, 16 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TNA008F tGCATCTCCCCCTACTGTACACCACc...cTTAGCACCTGGTTGGAGGGGGTTTATa
aCGTAGAGGGGGATGACATGTGGTGg...gAATCGTGGACCAACCTCCCCCAAATAt
    24+[19]+26
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA011 tATAAGGAACGTGCTGCTactcatcctagtcctgtTGCTGCCAAGCTATTTAATa
aTATTCCTTGCACGACGAtgagtaggatcaggacaACGACGGTTCGATAAATTAt
    17+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA012 tATTAAATAGCTTGGCAGCAa...tTGCTGCCAAGCTATTTAATa
aTAATTTATCGAACCGTCGTt...aACGACGGTTCGATAAATTAt
    19+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA013 tATGACACCCGGTGTGGGactcatcctagtcctgtTGCTGCCAAGCTATTTAATa
aTACTGTGGGCCACACCCtgagtaggatcaggacaACGACGGTTCGATAAATTAt
    17+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA014 tACTGTTGACCCAATGCGactcatcctagtcctgtTGCTGCCAAGCTATTTAATa
aTGACAACTGGGTTACGCtgagtaggatcaggacaACGACGGTTCGATAAATTAt
    17+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA015 tGCATATCCCCCAGCCAGACAAACa...tTGCTGCCAAGCTATTTAATa
aCGTATAGGGGGTCGGTCTGTTTGt...aACGACGGTTCGATAAATTAt
    23+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA016 tAATAACAATCAACCCACGTGGTTa...tTGCTGCCAAGCTATTTAATa
aTTATTGTTAGTTGGGTGCACCAAt...aACGACGGTTCGATAAATTAt
    23+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA017 tTCCAACCCAACCCTATCTTTCAa...tTGCTGCCAAGCTATTTAATa
aAGGTTGGGTTGGGATAGAAAGTt...aACGACGGTTCGATAAATTAt
    22+[17]+19
(artificial) (NS-N; others) +297 WT GG(Y) 21459844 R
TNA018 tGTCTACCAAGAACACCCTTAGGAa...tTGCTGCCAAGCTATTTAATa
aCAGATGGTTCTTGTGGGAATCCTt...aACGACGGTTCGATAAATTAt
    23+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA019 tTCCGCAAGCAAGCGTGGAATTAAAa...tTGCTGCCAAGCTATTTAATa
aAGGCGTTCGTTCGCACCTTAATTTt...aACGACGGTTCGATAAATTAt
    24+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TNA020 tACCTGGCAAGTGAGCAGCCCCACa...tTGCTGCCAAGCTATTTAATa
aTGGACCGTTCACTCGTCGGGGTGt...aACGACGGTTCGATAAATTAt
    23+[17]+19
(artificial) (N*; NS-N; others) +297 WT GG(Y) 21459844 R
TN0004 tATAAGGAACGTGCTGCTactcatcctagtcctgtTGCTGCCAAGCTATTTAATa
aTATTCCTTGCACGACGAtgagtaggatcaggacaACGACGGTTCGATAAATTAt
    17+[17]+19
yeast, URA3 G (N*; others) +297 WT GG(Y) NHEJ, HR 21459844 R
TN0005 tATGACACCCGGTGTGGGtttagatgacaagggagaCGCATTGGGTCAACAGTa
aTACTGTGGGCCACACCCaaatctactgttccctctGCGTAACCCAGTTGTCAt
    17+[18]+17
yeast, URA3 G (others) +297 WT GG(Y) NHEJ, HR 21459844 R
TN0006 tGCATATCCCCCAGCCAGACAAACc...aAACCACGTGGGTTGATTGTTATTa
aCGTATAGGGGGTCGGTCTGTTTGg...tTTGGTGCACCCAACTAACAATAAt
    23+[20]+23
yeast, LYS2 G (N*; others) +297 WT GG(Y) NHEJ 21459844 R
TN0007 tTCCAACCCAACCCTATCTTTCACa...tTCCTAAGGGTGTTCTTGGTAGACa
aAGGTTGGGTTGGGATAGAAAGTGt...aAGGATTCCCACAAGAACCATCTGt
    22+[18]+23
yeast, LYS2 G (others) +297 WT GG(Y) NHEJ 21459844 R
TN0008 tCCGCAAGCAAGCGTGGAATTAAAa...gGTGGGGCTGCTCACTTGCCAGGTa
aGGCGTTCGTTCGCACCTTAATTTt...cCACCCCGACGAGTGAACGGTCCAt
    24+[19]+23
yeast, ADE2 G (others) +297 WT GG(Y) NHEJ 21459844 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
GSearch EENdb with this gene symbol
RSearch EENdb with this reference

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