EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN first reported by the reference 21179091: 0 ZFN, 10 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TNA009 tATAAATACCTTCTn(2-24)AGAAGGTATTTATa
aTATTTATGGAAGAn(2-24)TCTTCCATAAATAt
    13+[2-24]+13
(artificial) (others) +28, +39, +50, +63, +79, +95 WT (N/A) 21179091 R
TN0001 cGGAGCCATCTGGCCGGGTtggctggttataACCGCGCAGATTCTGTTCa
gCCTCGGTAGACCGGCCCAaccgaccaatatTGGCGCGTCTAAGACAAGt
    18+[12]+18
human, NTF3, 5'-Up G C (NK) +63, +28 WT (N/A) NHEJ, NHEJ-ODC 21179091 R
TN0002 tCTTCATTACACCTg...cATACAGTCAGTatcaattctggaagaa
aGAAGTAATGTGGAc...gTATGTCAGTCAtagttaagaccttctt
    13+[16]+11
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0002B tctTCATTACACCTGCAGCTc...tACAGTCAGTATCAattctggaagaa
agaAGTAATGTGGACGTCGAg...aTGTCAGTCATAGTtaagaccttctt
    17+[12]+13
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0002C tcttcattACACCTGCAGCTCTc...cAGTATCAATTCTGGAAGaa
agaagtaaTGTGGACGTCGAGAg...gTCATAGTTAAGACCTTCtt
    14+[16]+17
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0002D tcttcattACACCTGCAGCTCTCATt...tATCAATTCTGGAAGAa
agaagtaaTGTGGACGTCGAGAGTAa...aTAGTTAAGACCTTCTt
    17+[16]+15
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0003 tGCTGGTCATCCTCATcctgataaactgcAAAAGGCTGAAGAGCatgactgaca
aCGACCAGTAGGAGTAggactatttgacgTTTTCCGACTTCTCGtactgactgt
    15+[13]+15
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TN0003B tgctGGTCATCCTCATCCTgataaactgcaaAAGGCTGAAGAGCatgactgaca
acgaCCAGTAGGAGTAGGActatttgacgttTTCCGACTTCTCGtactgactgt
    15+[12]+13
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TN0003C tgctggtCATCCTCATCCTGATaaactgcaaaAGGCTGAAGAGCATGactgaca
acgaccaGTAGGAGTAGGACTAtttgacgtttTCCGACTTCTCGTACtgactgt
    15+[10]+15
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TN0003D tgctggtcatCCTCATCCTGATAAACTgcaaaaggctgaAGAGCATGACTGACa
acgaccagtaGGAGTAGGACTATTTGAcgttttccgactTCTCGTACTGACTGt
    17+[12]+14
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
GSearch EENdb with this gene symbol
RSearch EENdb with this reference

©2012-2013  PKU Zebrafish Functional Genomics Group
School of Life Sciences, Peking University, Beijing, China
Reference of EENdb    Contact us