EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'y': 16 ZFN, 18 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0006 tGCATATCCCCCAGCCAGACAAACc...aAACCACGTGGGTTGATTGTTATTa
aCGTATAGGGGGTCGGTCTGTTTGg...tTTGGTGCACCCAACTAACAATAAt
    23+[20]+23
yeast, LYS2 G (N*; others) +297 WT GG(Y) NHEJ 21459844 R
TN0007 tTCCAACCCAACCCTATCTTTCACa...tTCCTAAGGGTGTTCTTGGTAGACa
aAGGTTGGGTTGGGATAGAAAGTGt...aAGGATTCCCACAAGAACCATCTGt
    22+[18]+23
yeast, LYS2 G (others) +297 WT GG(Y) NHEJ 21459844 R
TN0013 tATTTCGACGCTCATTCTctggccatggacttcttgAGCATCGGCTTCCGGGa
aTAAAGCTGCGAGTAAGAgaccggtacctgaagaacTCGTAGCCGAAGGCCCt
    17+[18]+16
zebrafish, hey2 G C (std.) +231 WT GG(V&B) 21493687 R
TN0033 tCTTCCGTTTCCACATCCaccacatcccaacagagcAGCGGGAGCAGCAGTAAa
aGAAGGCAAAGGTGTAGGtggtgtagggttgtctcgTCGCCCTCGTCGTCATTt
    17+[18]+17
zebrafish, hey2 G C (others) +63 WT REAL NHEJ 21822241 R
TN0062 tAATATCACAATGAGTTCaggcttatggagccaagaAAAAGTCACTTCACCCTa
aTTATAGTGTTACTCAAGtccgaatacctcggttctTTTTCAGTGAAGTGGGAt
    17+[18]+17
human, CYLD, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0101 tCACTGTCGGCGGCCATGacaccgctcgtctcccgcCTGAGTCGTCTGTGGGTa
aGTGACAGCCGCCGGTACtgtggcgagcagagggcgGACTCAGCAGACACCCAt
    17+[18]+17
human, MUTYH, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0102 tGCTTAGACGCTGGATTTttttcgggtagtggaaAACCAGGTAAGCACCGAa
aCGAATCTGCGACCTAAAaaaagcccatcaccttTTGGTCCATTCGTGGCTt
    17+[16]+17
human, MYC G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0103 tCCCGCAGGGAGCGGACAtggactacgactcgtaCCAGCACTATTTCTACGa
aGGGCGTCCCTCGCCTGTacctgatgctgagcatGGTCGTGATAAAGATGCt
    17+[16]+17
human, MYCL1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0104 tGCCGAGCTGCTCCACgtccaccatgccgggcATGATCTGCAAGAACCCa
aCGGCTCGACGAGGTGcaggtggtacggcccgTACTAGACGTTCTTGGGt
    15+[16]+17
human, MYCN, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0140 tACCACCACTAATCCGTgcccctatgcggtaaATGTCTTTGACTTGACCa
aTGGTGGTGATTAGGCAcggggatacgccattTACAGAAACTGAACTGGt
    16+[15]+17
fruitfly, y G (std.) +63 Sharkey-AS+R UA NHEJ 22624882 R
TN0146 tGCTCAAACCCGACGAGCatcaccacatcgaggacgAGCACGTGAGGGCGCCCa
aCGAGTTTGGGCTGCTCGtagtggtgtagctcctgcTCGTGCACTCCCGCGGGt
    17+[18]+17
zebrafish, myod1 G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0162 tGGACAATTCATCCGGCATccaagcagaggtcagAGGGTGAGAAGGTGCGCa
aCCTGTTAAGTAGGCCGTAggttcgtctccagtcTCCCACTCTTCCACGCGt
    18+[15]+17
zebrafish, ryr3 G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0164 tGGACCATTCACCCTGCATccaagcagaggtcagAGGGTGAGAAGGTCAGa
aCCTGGTAAGTGGGACGTAggttcgtctccagtcTCCCACTCTTCCAGTCt
    18+[15]+16
zebrafish, ryr1a G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0171 tATACATTTTACACACATATatatgaaactgacagtATTAATGGCCTGAACCTa
aTATGTAAAATGTGTGTATAtatactttgactgtcaTAATTACCGGACTTGGAt
    19+[16]+17
pig, SRY, 3'-downstream 1.4kb G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0172 tATATGAAACTGACAGTATtaatggcctgaacctAGCCAGAACTCAGATTGGGa
aTATACTTTGACTGTCATAattaccggacttggaTCGGTCTTGAGTCTAACCCt
    18+[15]+19
pig, SRY, 3'-downstream 1.5kb G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0179 tCCTTCCAAGGCTTCTACATc...cACTTTTGATAAGACATTCTTGGTa
aGGAAGGTTCCGAAGATGTAg...gTGAAAACTATTCTGTAAGAACCAt
    19+[16]+23
pig, AMELY, Intron G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0180 tCTACATCAATCACTTGGCTttcacttttgataagACATTCTTGGTATGGATa
aGATGTAGTTAGTGAACCGAaagtgaaaactattcTGTAAGAACCATACCTAt
    19+[15]+17
pig, AMELY, Intron G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R No
TN0205 tCCAAGCACTGTTTAAGTctggatgttggtcaaaACCCAATTTGATGATGTa
aGGTTCGTGACAAATTCAgacctacaaccagtttTGGGTTAAACTACTACAt
    17+[16]+17
cow, OFD1Y G (std.) +63-Goldy WT GG(V&B) 23027955 R No



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0001 tGCCTACCGCattaaaGTGGATGAGt
aCGGATGGCGtaatttCACCTACTCa
    3F+[6]+3F
fruitfly, y G ZP10002 ZP10001 QNKK WT MA NHEJ 12136019 R
ZN0005 gTTCCCCTTCaaagggGTGGATGACc
cAAGGGGAAGtttcccCACCTACTGg
    3F+[6]+3F
mouse, Tyr G ZP10010 ZP10009 TG WT MA 16084494 R
ZN0010 tAGCTACTACacgaatGGCGTGGGAg
aTCGATGATGtgcttaCCGCACCCTc
    3F+[6]+3F
fruitfly, ry G ZP10025 ZP10024 QNKK WT MA NHEJ; HR 16452139 R
ZN0035 aCCCCTACACctgctGCAGGAGGTg
tGGGGATGTGgacgaCGTCCTCCAc
    3F+[5]+3F
zebrafish, hif1ab G C ZP10358 ZP10359 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0036 aTCCTGCTGCgttcctGCAGCAGAGc
tAGGACGACGcaaggaCGTCGTCTCg
    3F+[6]+3F
zebrafish, hey2 G C ZP10385 ZP10388 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0017-T017 gTCCCTCCCTaagggtGATGGGGTGt
cAGGGAGGGAttcccaCTACCCCACa
    3F+[6]+3F
zebrafish, adcyap1b, 3'-UTR G C ZP10047-T017 ZP10046-T014 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T020 cTCCCACCAGgatatccgggttacGCAGGTGTGg
gAGGGTGGTCctataggcccaatgCGTCCACACc
    3F+[14]+3F
zebrafish, myo5aa G C ZP10047-T019 ZP10046-T002 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0114 gGCGGCGTGCTCGcggtgcGGAGACCGGAAGg
cCGCCGCACGAGCgccacgCCTCTGGCCTTCc
    4F+[6]+4F
mouse, Thumpd3, 5'-UTR G ZP10699 ZP10700 TGEK WT MA(2F) NHEJ 21191366 R
ZN0141 cCACATCCCAacagaGCAGCGGGAg
gGTGTAGGGTtgtctCGTCGCCCTc
    3F+[5]+3F
zebrafish, hey2 G C ZP10760 ZP10761 LRGS EL+KK (N/A) NHEJ 21822241 R
ZN0177 cACACACCTGtgagtcCAGGAGGACg
gTGTGTGGACactcagGTCCTCCTGc
    3F+[6]+3F
zebrafish, yrk G C ZP10841 ZP10842 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0178 cCTAGCTCCTGGACTGACCactattGGAGCCTGTAAGTATACTGg
gGATCGAGGACCTGACTGGtgataaCCTCGGACATTCATATGACc
    6F+[6]+(1+6F)
human, DMD G C ZPU0043 ZPU0044 (N/A) (N/A) Sangamo NHEJ 21954090 R
ZN0191 aAACCATACCgaccgtGGGGAACTGa
tTTGGTATGGctggcaCCCCTTGACt
    3F+[6]+3F
zebrafish, hey2 G C ZP10864 ZP10865 LRGS D+R 2F-MA NHEJ 22543349 R Low
ZN0202 gAGCATCACCacatcGAGGACGAGc
cTCGTAGTGGtgtagCTCCTGCTCg
    3F+[5]+3F
zebrafish, myod1 G C ZP10886 ZP10887 (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZNU013 (N/A)
    
zebrafish, yap G C (N/A) (N/A) (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZN0207 aAGCCACAGTCTCCTGGatgggtGGCACCGGAGATGAGa
tTCGGTGTCAGAGGACCtacccaCCGTGGCCTCTACTCt
    (1+5F)+[6]+5F
rainbow trout, sdY G ZPU0059 ZPU0060 (N/A) (N/A) Sangamo NHEJ 22727696 R
ZN0218 gACCCTGAGCCCTacggcCGGGTCCGGGACc
cTGGGACTCGGGAtgccgGCCCAGGCCCTGg
    4F+[5]+4F
butterfly, cry2 G ZPU0075 ZPU0076 (N/A) D+R 2F-MA NHEJ 23009861 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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