EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'w': 4 ZFN, 5 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0014 tCCTGACCATTCATCAGCCGTCTt...cAAGATCCTTCTGATGGCCGAGGGCa
aGGACTGGTAAGTAGTCGGCAGAa...gTTCTAGGAAGACTACCGGCTCCCGt
    22+[24]+24
fruitfly, w G (others) +231 WT GG(V&B) 21493687 R
TN0066 tGGCGTCCACGGGTGAGTatggtggaactgcggtcGCGCCGGCGGTAGCCGGa
aCCGCAGGTGCCCACTCAtaccaccttgacgccagCGCGGCCGCCATCGGCCt
    17+[17]+17
human, EWSR1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0138 tCTTCCTTCCTAGCACTATATAAAc...cTAAGCTCATCAAGCCTTCAAGCa
aGAAGGAAGGATCGTGATATATTTg...gATTCGAGTAGTTCGGAAGTTCGt
    23+[18]+22
rice, Os11N3, promoter G (std.) +297 WT GG-PCR NHEJ 22565958 R
TN0139 tATAAACCCCCTCCAACCAGGTGCTAAg...cAAAGCAAACTCAAGTAGTAGCTGa
aTATTTGGGGGAGGTTGGTCCACGATTc...gTTTCGTTTGAGTTCATCATCGACt
    26+[19]+23
rice, Os11N3, promoter G (N*; NS-N; others) +297 WT GG-PCR NHEJ 22565958 R
TNT21 tCACACCGGAGCGCCAtacggctcgctggcccaGCACTCCTTCATCAa
aGTGTGGCCTCGCGGTatgccgagcgaccgggtCGTGAGGAAGTAGTt
    15+[17]+14
zebrafish, wt1a G C (std.) +63 Sharkey-AS+R UA NHEJ, NHEJ-DisDel T1



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0011 tCCCATCATCaggcggGAGGTGGGCg
aGGGTAGTAGtccgccCTCCACCCGc
    3F+[6]+3F
fruitfly, bw G ZP10022 ZP10023 QNKK WT MA NHEJ 16452139 R
ZN0035 aCCCCTACACctgctGCAGGAGGTg
tGGGGATGTGgacgaCGTCCTCCAc
    3F+[5]+3F
zebrafish, hif1ab G C ZP10358 ZP10359 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0089 aGTTACTTACacgtggGCCGATGTCa
tCAATGAATGtgcaccCGGCTACAGt
    3F+[6]+3F
silk worm, Bmwh3 G ZP10551 ZP10550 TGQK WT MA NHEJ 20692340 R
ZN0114 gGCGGCGTGCTCGcggtgcGGAGACCGGAAGg
cCGCCGCACGAGCgccacgCCTCTGGCCTTCc
    4F+[6]+4F
mouse, Thumpd3, 5'-UTR G ZP10699 ZP10700 TGEK WT MA(2F) NHEJ 21191366 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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