EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'hey2 gridlock': 3 ZFN, 2 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0013 tATTTCGACGCTCATTCTctggccatggacttcttgAGCATCGGCTTCCGGGa
aTAAAGCTGCGAGTAAGAgaccggtacctgaagaacTCGTAGCCGAAGGCCCt
    17+[18]+16
zebrafish, hey2 G C (std.) +231 WT GG(V&B) 21493687 R
TN0033 tCTTCCGTTTCCACATCCaccacatcccaacagagcAGCGGGAGCAGCAGTAAa
aGAAGGCAAAGGTGTAGGtggtgtagggttgtctcgTCGCCCTCGTCGTCATTt
    17+[18]+17
zebrafish, hey2 G C (others) +63 WT REAL NHEJ 21822241 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0036 aTCCTGCTGCgttcctGCAGCAGAGc
tAGGACGACGcaaggaCGTCGTCTCg
    3F+[6]+3F
zebrafish, hey2 G C ZP10385 ZP10388 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0141 cCACATCCCAacagaGCAGCGGGAg
gGTGTAGGGTtgtctCGTCGCCCTc
    3F+[5]+3F
zebrafish, hey2 G C ZP10760 ZP10761 LRGS EL+KK (N/A) NHEJ 21822241 R
ZN0191 aAACCATACCgaccgtGGGGAACTGa
tTTGGTATGGctggcaCCCCTTGACt
    3F+[6]+3F
zebrafish, hey2 G C ZP10864 ZP10865 LRGS D+R 2F-MA NHEJ 22543349 R Low




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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School of Life Sciences, Peking University, Beijing, China
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