EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'gria3a': 1 ZFN, 3 TALEN, 1 CRISPR/Cas records match.
(Click on ID to see details)


CRISPR/Cas IDWhole SiteSpecies & LocusPAMRNA OligoModif.Ref.Effic.
CN0011 GGTGGTATTTTTTTGAGTGTggggg
CCACCATAAAAAAACTCACAccccc
    20
zebrafish, gria3a, intron G C NGG gRNA NHEJ 23360964 R No



TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0034 tCGTCCAATAGCTTCTcagtcacgcacgcctgtGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAgtcagtgcgtgcggacaCTCAAAGACGAGAAAt
    15+[17]+15
zebrafish, gria3a G C (std.) +63 WT REAL NHEJ 21822241 R
TN0034B tCGTCCAATAGCTTCTcagtcacgcacgcctgTGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAgtcagtgcgtgcggacACTCAAAGACGAGAAAt
    15+[16]+16
zebrafish, gria3a G C (std.) +63 WT REAL NHEJ 21822241 R
TN0034C tCGTCCAATAGCTTCTCagtcacgcacgcctgtGAGTTTCTGCTCTTTa
aGCAGGTTATCGAAGAGtcagtgcgtgcggacaCTCAAAGACGAGAAAt
    16+[16]+15
zebrafish, gria3a G C (std.) +63 WT REAL NHEJ 21822241 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0142 tAGCTTCTCAGTCacgcacGCCTGTGAGTTTc
aTCGAAGAGTCAGtgcgtgCGGACACTCAAAg
    4F+[6]+4F
zebrafish, gria3a G C ZP10763 ZP10762 LRGS EL+KK (N/A) NHEJ 21822241 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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School of Life Sciences, Peking University, Beijing, China
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