EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'TP53': 5 ZFN, 1 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0133 tTGCCGTCCCAAGCAATGgatgatttgatgctgtcCCCGGACGATATTGAACa
aACGGCAGGGTTCGTTACctactaaactacgacagGGGCCTGCTATAACTTGt
    17+[17]+17
human, TP53 G C (std.) +63 WT FLASH NHEJ 22484455 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0130 tCCCCTGCTTGGCtgggcgCAGTGGCTCATGc
aGGGGACGAACCGacccgcGTCACCGAGTACg
    4F+[6]+4F
human, TP53, intron G C ZP10734 ZP10735 QNKK WT, EL+KK MMA NHEJ; HR 21695267 R
ZN0131 cCACCATCCACTAcaactaCATGTGTAACAGt
gGTGGTAGGTGATgttgatGTACACATTGTCa
    4F+[6]+4F
human, TP53 G C ZP10736 ZP10737 QNKK WT, EL+KK MMA NHEJ; HR 21695267 R
ZN0179 cCGCGTCCGCGCCatggcCATCTACAAGCAg
gGCGCAGGCGCGGtaccgGTAGATGTTCGTc
    4F+[5]+4F
human, TP53 G C ZP10845 ZP10846 TGEK Sharkey-AS+R MA(2F) NHEJ 21983922 R
ZNU015 (N/A)
    
zebrafish, tp53 G C (N/A) (N/A) (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZN0210 gTTCTCCAGTCTTCCTCCAGatgatATTCTGGTAAGGAGCCGGg
cAAGAGGTCAGAAGGAGGTCtactaTAAGACCATTCCTCGGCCc
    (1+6F)+[5]+6F
rat, Tp53 G ZPU0065 ZPU0066 (N/A) (N/A) Sangamo NHEJ 22917926 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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School of Life Sciences, Peking University, Beijing, China
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