EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'SuRB': 3 ZFN, 2 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0022 tACTGAATAATCAACACTtgggaatggtggttcAATGGGAGGATCGGTTCTATa
aTGACTTATTAGTTGTGAacccttaccaccaagTTACCCTCCTAGCCAAGATAt
    17+[15]+20
tobacco, SuRB G (std.) +231 WT GG(V&B) 21493687 R
TN0023 tACCTCATCAGGAACATGTTctacctatgattcccAGTGGCGGGGCTTTCAAAGa
aTGGAGTAGTCCTTGTACAAgatggatactaagggTCACCGCCCCGAAAGTTTCt
    19+[15]+19
tobacco, SuRB G (std.) +231 WT GG(V&B) 21493687 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0028 gGAATCCTTCtaatgaGGCGGAGATc
cCTTAGGAAGattactCCGCCTCTAg
    3F+[6]+3F
tobacco, SuRB/SuRA G ZP10335 ZP10337 LRGS WT OPEN NHEJ 18657511 R
ZN0037 cTACCACGTCacgagcAGGGTGGTGt
gATGGTGCAGtgctcgTCCCACCACa
    3F+[6]+3F
tobacco, SuRB G ZP10392 ZP10393 LRGS WT, D+R MA NHEJ; HR 19404258 R
ZN0038 gGGCAGCACCagatgTGGGCTGCTc
cCCGTCGTGGtctacACCCGACGAg
    3F+[5]+3F
tobacco, SuRB G ZP10400 ZP10406 LRGS EL+KK OPEN NHEJ; HR 19404258 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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