EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'REST': 2 ZFN, 1 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0118 tCAGAATACAGTTATGGCcacccaggtaatggggCAGTCTTCTGGAGGAGGa
aGTCTTATGTCAATACCGgtgggtccattaccccGTCAGAAGACCTCCTCCt
    17+[16]+17
human, REST, ATG G C (std.) +63 WT FLASH 22484455 R No



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0214 tGTCTGCTCCtcagctGGTGATGCTg
aCAGACGAGGagtcgaCCACTACGAc
    3F+[6]+3F
zebrafish, rest G C ZP10901, ZP10902 ZP10903 (N/A) (N/A) (N/A) NHEJ 22951640 R
ZN0215 cAGCTACTCCgacagcGAAGAAGACg
gTCGATGAGGctgtcgCTTCTTCTGc
    3F+[6]+3F
zebrafish, rest G C ZP10905, ZP10906 ZP10907 (N/A) (N/A) (N/A) NHEJ 22951640 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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RSearch EENdb with this reference

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