EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'PITX3': 1 ZFN, 2 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0026 tCAGACGCTGGCACTccgcacccccagctcccagagcACGGCTGCAAGGGCCa
aGTCTGCGACCGTGAggcgtgggggtcgagggtctcgTGCCGACGTTCCCGGt
    14+[22]+15
human, PITX3 G C (std.) +63 WT (N/A) NHEJ, HR 21738127 R
TN0027 tACGCCGTGGAAAGGCCcgtatgagcggccccgcccGTAGATCATCCCCGa
aTGCGGCACCTTTCCGGgcatactcgccggggcgggCATCTAGTAGGGGCt
    16+[19]+14
human, PITX3, 3'-UTR G C (std.) +63 WT (N/A) NHEJ, HR 21738127 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0061 cCCCAGCTCCCAGAGCacggctGCAAGGGCCaGGAGCAc
gGGGTCGAGGGTCTCGtgccgaCGTTCCCGGtCCTCGTg
    5F+[6]+(3F+1+2F)
human, PITX3 G C ZP10477 ZP10476 LRGS EL+KK Sangamo NHEJ; HR 19680244 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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