EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'MSTN GDF8': 1 ZFN, 5 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0196 tCCCTTTTTAGAAGTCAAGGTa...aAAGATCCAGGAGAGATTTTGGa
aGGGAAAAATCTTCAGTTCCAt...tTTCTAGGTCCTCTCTAAAACCt
    20+[15]+21
pig, MSTN G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0197 tGGATTATTGCACCCAAAAGATa...gCTCTGGAGAGTGTGAATTTGTa
aCCTAATAACGTGGGTTTTCTAt...cGAGACCTCTCACACTTAAACAt
    21+[16]+21
pig, MSTN G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0198 tCTAACTGTGGATTTTGAAGCTtttggatgggactggATTATTGCACCCAAAAGa
aGATTGACACCTAAAACTTCGAaaacctaccctgaccTAATAACGTGGGTTTTCt
    21+[15]+17
pig, MSTN G (std.) +63-Goldy, +231 WT GG(V&B) NHEJ 23027955 R Low
TN0199A tGTGATGAACACTCCACAGAATCTc...cCTCTAACTGTGGATTTTGAa
aCACTACTTGTGAGGTGTCTTAGAg...gGAGATTGACACCTAAAACTt
    23+[16]+19
cow, MSTN G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0199B tGTGATGAACACTCCACAGAATCTc...aACTGTGGATTTTGAAGCTTTTGGa
aCACTACTTGTGAGGTGTCTTAGAg...tTGACACCTAAAACTTCGAAAACCt
    23+[21]+23
cow, MSTN G (std.) +63-Goldy WT GG(V&B) 23027955 R No



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0185 aACCCACTGCcgtgacGGAGGAGCGt
tTGGGTGACGgcactgCCTCCTCGCa
    3F+[6]+3F
yellow catfish, mstn G ZP10854 ZP10855 (N/A) EL+KK MA NHEJ 22194943 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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