EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'IL2RG': 2 ZFN, 3 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0030 tACACGTTTCGTGTTCGGAgccgctttaacccacTCTGTGGAAGTGCTCAGCa
aTGTGCAAAGCACAAGCCTcggcgaaattgggtgAGACACCTTCACGAGTCGt
    18+[15]+18
human, IL2RG G C (std.) +47, +17 KV+EA (N/A) NHEJ 21813459 R
TN0192 tCCCAAAGGTTCAGTGTTTtgtgttcaatgttgAGTACATGAATTGCACTTGGa
aGGGTTTCCAAGTCACAAAacacaagttacaacTCATGTACTTAACGTGAACCt
    18+[14]+20
pig, IL2RG G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0193 tCTACCAAACATTTGTTGTccagctccaggacccACGGGAACCCAGGAGGCAGGa
aGATGGTTTGTAAACAACAggtcgaggtcctgggTGCCCTTGGGTCCTCCGTCCt
    18+[15]+20
pig, IL2RG G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R No



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0003 tCGGAGCCGCTTTaacccACTCTGTGGAAGt
aGCCTCGGCGAAAttgggTGAGACACCTTCa
    4F+[5]+4F
human, IL2RG G C ZP10005 ZP10006 (N/A) WT Sangamo HR 15806097 R
ZN0075 aCCAACCTCACTATGCactataGGTATGAGAAGGGGGa
tGGTTGGAGTGATACGtgatatCCATACTCTTCCCCCt
    5F+[6]+5F
rat, Il2rg, exon-intron G ZPU0005 ZPU0006 LRGS EL+KK Sangamo NHEJ 20111598 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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