EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'GGTA1': 2 ZFN, 3 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0181 tCTGCGCTCCTTCAAAGTgtttaagatcaagcctgAGAAGAGGTGGCAGGACa
aGACGCGAGGAAGTTTCAcaaattctagttcggacTCTTCTCCACCGTCCTGt
    17+[17]+17
cow, GGTA1 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0182 tCTGCAAATACATACTTCATg...cATCTTTTACATCATGGTGGa
aGACGTTTATGTATGAAGTAc...gTAGAAAATGTAGTACCACCt
    19+[16]+19
pig, GGTA1 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0183 tGCGTTCCTTTAAAGTGTttgagatcaagtccgAGAAGAGGTGGCAAGACa
aCGCAAGGAAATTTCACAaactctagttcaggcTCTTCTCCACCGTTCTGt
    17+[15]+17
pig, GGTA1 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0137 tCGGTGGCTCAGCTACaggcctGGTGGTACAAGGCACa
aGCCACCGAGTCGATGtccggaCCACCATGTTCCGTGt
    5F+[6]+5F
pig, GGTA1 G ZP10753 ZP10754 LRGS EL+KK Sangamo NHEJ 21730124 R
ZN0208 nGTCATCTTTTACATCatggtgGATGATATCTCCAGGATGCCn
nCAGTAGAAAATGTAGtaccacCTACTATAGAGGTCCTACGGn
    5F+[6]+(2+6F)
pig, GGTA1 G ZPU0061 ZPU0062 (N/A) (N/A) Sangamo NHEJ 22795273 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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