EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'FH': 1 ZFN, 2 TALEN, 2 CRISPR/Cas records match.
(Click on ID to see details)


CRISPR/Cas IDWhole SiteSpecies & LocusPAMRNA OligoModif.Ref.Effic.
CN0003 GGAGCGGTACATGGCGACCGggggc
CCTCGCCATGTACCGCTGGCccccg
    20
zebrafish, fh G C NGG gRNA NHEJ 23360964 R
CN0004 GGAGCGAGCGGAGCGGTACAtggcg
CCTCGCTCGCCTCGCCATGTaccgc
    20
zebrafish, fh G C NGG gRNA NHEJ 23360964 R



TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0077 tGTACCGAGCACTTCGGCtcctcgcgcgctcgcgtCCCCTCGTGCGGGCTCCa
aCATGGCTCGTGAAGCCGaggagcgcgcgagcgcaGGGGAGCACGCCCGAGGt
    17+[17]+17
human, FH, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0151 tTTGATGGATCTCTGAGCggcccgcagatctgacAAACTCGCGCTGAAGCGa
aAACTACCTAGAGACTCGccgggcgtctagactgTTTGAGCGCGACTTCGCt
    17+[16]+17
zebrafish, fh G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0017-T005 tCCCGACCAGattgtGAAGGTGTGt
aGGGCTGGTCtaacaCTTCCACACa
    3F+[5]+3F
zebrafish, fhdc1 G C ZP10047-T005 ZP10046 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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School of Life Sciences, Peking University, Beijing, China
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