EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'CFTR': 2 ZFN, 2 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0011 tGGATTATGCCTGGCACCATTAAAGAAAATa...tATAGATACAGAAGCGTCATCAAAGCATGCCa
aCCTAATACGGACCGTGGTAATTTCTTTTAt...aTATCTATGTCTTCGCAGTAGTTTCGTACGGt
    29+[28]+30
human, CFTR G C (others) +231 WT GG(V&B) 21493687 R
TN0012 tGGCACCATTAAAGAAAATATCATc...tAGATACAGAAGCGTCATCAAAGCATGCCa
aCCGTGGTAATTTCTTTTATAGTAg...aTCTATGTCTTCGCAGTAGTTTCGTACGGt
    23+[25]+28
human, CFTR G C (std.) +231 WT GG(V&B) (Weak) 21493687 R Low



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0004 cTGACTCCTGatgttgattTTGGGAGAAc
gACTGAGGACtacaactaaAACCCTCTTg
    3F+[9]+3F
mouse, Cftr G ZP10008 ZP10007 TG(G4S)3 WT MA 16084494 R
ZN0022 tCACTTCTAAtggtgaTTATGGGAGa
aGTGAAGATTaccactAATACCCTCt
    3F+[6]+3F
human, CFTR, intron G C ZP10153 ZP10165 LRGS WT OPEN NHEJ 18657511 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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