EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'CCR5': 20 ZFN, 10 TALEN, 0 CRISPR/Cas records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0002 tCTTCATTACACCTg...cATACAGTCAGTatcaattctggaagaa
aGAAGTAATGTGGAc...gTATGTCAGTCAtagttaagaccttctt
    13+[16]+11
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0002B tctTCATTACACCTGCAGCTc...tACAGTCAGTATCAattctggaagaa
agaAGTAATGTGGACGTCGAg...aTGTCAGTCATAGTtaagaccttctt
    17+[12]+13
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0002C tcttcattACACCTGCAGCTCTc...cAGTATCAATTCTGGAAGaa
agaagtaaTGTGGACGTCGAGAg...gTCATAGTTAAGACCTTCtt
    14+[16]+17
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0002D tcttcattACACCTGCAGCTCTCATt...tATCAATTCTGGAAGAa
agaagtaaTGTGGACGTCGAGAGTAa...aTAGTTAAGACCTTCTt
    17+[16]+15
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ 21179091 R
TN0003 tGCTGGTCATCCTCATcctgataaactgcAAAAGGCTGAAGAGCatgactgaca
aCGACCAGTAGGAGTAggactatttgacgTTTTCCGACTTCTCGtactgactgt
    15+[13]+15
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TN0003B tgctGGTCATCCTCATCCTgataaactgcaaAAGGCTGAAGAGCatgactgaca
acgaCCAGTAGGAGTAGGActatttgacgttTTCCGACTTCTCGtactgactgt
    15+[12]+13
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TN0003C tgctggtCATCCTCATCCTGATaaactgcaaaAGGCTGAAGAGCATGactgaca
acgaccaGTAGGAGTAGGACTAtttgacgtttTCCGACTTCTCGTACtgactgt
    15+[10]+15
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TN0003D tgctggtcatCCTCATCCTGATAAACTgcaaaaggctgaAGAGCATGACTGACa
acgaccagtaGGAGTAGGACTATTTGAcgttttccgactTCTCGTACTGACTGt
    17+[12]+14
human, CCR5 G C (std.) +63, +28 WT (N/A) NHEJ, HR 21179091 R
TN0031 tGTGGGCAACATGCTGGTCatcctcatcctgataAACTGCAAAAGGCTGAAGa
aCACCCGTTGTACGACCAGtaggagtaggactatTTGACGTTTTCCGACTTCt
    18+[15]+18
human, CCR5 G C (std.) +47, +17 KV+EA (N/A) NHEJ 21813459 R
TN0037 tGCATCAACCCCATCATCtatgcctttgtcGGGGAGAAGTTCAGAAACTa
aCGTAGTTGGGGTAGTAGatacggaaacagCCCCTCTTCAAGTCTTTGAt
    17+[12]+19
human, CCR5 G C (N/A) (N/A) (N/A) NHEJ 21983922 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0006 tGTCCCCTTCtgggctcactatGCTGCCGCCc
aCAGGGGAAGacccgagtgataCGACGGCGGg
    3F+[12]+3F
human, CCR5 G C ZP10013 ZP10014 TG(G4S)3 WT MA 16084494 R
ZN0019 gGTCATCCTCATCctgatAAACTGCAAAAGg
cCAGTAGGAGTAGgactaTTTGACGTTTTCc
    4F+[5]+4F
human, CCR5 G C ZP10064 ZP10065 LRGS WT, EL+KK Sangamo NHEJ 18587387 R
ZN0043 aAGTCCAATCTATgacatcAATTATTATACAt
tTCAGGTTAGATActgtagTTAATAATATGTa
    4F+[6]+4F
human, CCR5 G C ZP10438 ZP10439 TGEK WT MA NHEJ 19470664 R
ZN0044 tCTGGGCTCACTAtgctgcCGCCCAGTGGGAc
aGACCCGAGTGATacgacgGCGGGTCACCCTg
    4F+[6]+4F
human, CCR5 G C ZP10440 ZP10441 TGEK WT MA NHEJ 19470664 R
ZN0045 cTTCTTCATCATCctcctGACAATCGAt
gAAGAAGTAGTAGgaggaCTGTTAGCTa
    4F+[5]+3F
human, CCR5/CCR2 G C ZP10442 ZP10443 TGEK WT MA NHEJ 19470664 R
ZN0046 cTGTGTTTGCTTTaaaagcCAGGACGGTCACc
gACACAAACGAAAttttcgGTCCTGCCAGTGg
    4F+[6]+4F
human, CCR5/CCR2 G C ZP10444 ZP10445 TGEK WT MA NHEJ 19470664 R
ZN0047 cAGGACGGTCacctttGGGGTGGTGa
gTCCTGCCAGtggaaaCCCCACCACt
    3F+[6]+3F
human, CCR5 G C ZP10446 ZP10447 TGEK WT MA NHEJ 19470664 R
ZN0048 aCGGTCACCTttggggTGGTGACAAGTGt
tGCCAGTGGAaaccccACCACTGTTCACa
    3F+[6]+4F
human, CCR5/CCR2 G C ZP10448 ZP10449 TGEK WT MA NHEJ 19470664 R
ZN0049 aCCAAGCTATgcaggtGACAGAGACt
tGGTTCGATAcgtccaCTGTCTCTGa
    3F+[6]+3F
human, CCR5 G C ZP10450 ZP10451 TGEK WT MA NHEJ 19470664 R
ZN0050 cCCCATCATCTATgcctttGTCGGGGAGAAGt
gGGGTAGTAGATAcggaaaCAGCCCCTCTTCa
    4F+[6]+4F
human, CCR5 G C ZP10452 ZP10453 TGEK WT, D+R, EL+KK MA NHEJ 19470664 R
ZN0055 tACTGTCCCCTTCtgggctcactatGCTGCCGCCCAGt
aTGACAGGGGAAGacccgagtgataCGACGGCGGGTCa
    4F+[12]+4F
human, CCR5 G C ZP10465 ZP10464 QNKKGLD(G4S)3 WT, D+R, EL+KK MA NHEJ 19635463 R
ZN0056 aGCTCTCATTTTCcatacaGTCAGTATCAATt
tCGAGAGTAAAAGgtatgtCAGTCATAGTTAa
    4F+[6]+4F
human, CCR5 G C ZP10467 ZP10466 QNKK WT, D+R, EL+KK MA NHEJ 19635463 R
ZN0065 aGGCTCCTCCTCCaacattGGGGATTATGATt
tCCGAGGAGGAGGttgtaaCCCCTAATACTAa
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10484 ZP10485 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0066 cTCATCCATTTGCactcctGGGTATGGAGACc
gAGTAGGTAAACGtgaggaCCCATACCTCTGg
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10486 ZP10487 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0067 cATACCCACCTCAcgtgtgGTCAATGGAGTGt
gTATGGGTGGAGTgcacacCAGTTACCTCACa
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10488 ZP10489 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0068 gGTCCCATCACCAgcgatgGATATAGTCTGGa
cCAGGGTAGTGGTcgctacCTATATCAGACCt
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10490 ZP10491 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0069 gGTCTGCAGCCACagctggGTGGATGCAGCTg
cCAGACGTCGGTGtcgaccCACCTACGTCGAc
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10492 ZP10493 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0070 tGACCTCACCACCtctcagTGGGAAGAGGAGg
aCTGGAGTGGTGGagagtcACCCTTCTCCTCc
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10494 ZP10495 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0071 tTTCTATGGCAGCctcaggGGAGAATGGGACt
aAAGATACCGTCGgagtccCCTCTTACCCTGa
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10496 ZP10497 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0121 gGTCGTCCTCATCctgatAAACTGCAAAAGg
cCAGCAGGAGTAGgactaTTTGACGTTTTCc
    4F+[5]+4F
macaque, CCR5 G ZP10717 ZP10065 LRGS EL+KK Sangamo NHEJ 21533216 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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