EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN targeting gene with symbol containing 'APOE': 2 ZFN, 1 TALEN, 1 CRISPR/Cas records match.
(Click on ID to see details)


CRISPR/Cas IDWhole SiteSpecies & LocusPAMRNA OligoModif.Ref.Effic.
CN0006 GGATGAGCCAAGAAGCCGCTgggaa
CCTACTCGGTTCTTCGGCGAccctt
    20
zebrafish, apoea G C NGG gRNA NHEJ 23360964 R



TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0202 tGTTGAGTGACAATTTCTCCCTCCTGCAGGTt...gATGAGGGTTCTGTGGGTTGCTTTGGTGGTa
aCAACTCACTGTTAAAGAGGGAGGACGTCCAa...cTACTCCCAAGACACCCAACGAAACCACCAt
    30+[21]+29
pig, APOE, intron-exon G (std.) +63-Goldy WT GG(V&B) 23027955 R No



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0104 gCCCCTCAGCccagaTGGGAGGAGa
cGGGGAGTCGggtctACCCTCCTCt
    3F+[5]+3F
zebrafish, apoeb G C ZP10623 ZP10688 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0198 gAACCTACGCACCtctctGGAGGGCCGTGTg
cTTGGATGCGTGGagagaCCTCCCGGCACAc
    4F+[5]+4F
zebrafish, apoeb G C ZP10878 ZP10879 LRGS D+R 2F-MA NHEJ 22543349 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
GSearch EENdb with this gene symbol
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