EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN with FokI variants ELD+KKR: 18 ZFN, 14 TALEN records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0025 tCCCCTCCACCCCACAGTggggccactagggacAGGATTGGTGACAGAAAa
aGGGGAGGTGGGGTGTCAccccggtgatccctgTCCTAACCACTGTCTTTt
    17+[15]+17
human, PPP1R12C, intron G C (std.) +63 WT; ELD+KKR (N/A) NHEJ, HR 21738127 R
TN0025-T002 tTCCACCCAGCCCTCAGTgctctgctgaggcAGGATGGGTGGCTGGAAa
aAGGTGGGTCGGGAGTCAcgagacgactccgTCCTACCCACCGACCTTt
    17+[13]+17
human, [intergenic] C +63 ELD+KKR (N/A) NHEJ 21738127 R
TN0025-T003 cCCCCTCCACCCCAAAAAt...aAATATGAGTTATAGAAAa
gGGGGAGGTGGGGTTTTTa...tTTATACTCAATATCTTTt
    17+[24]+17
human, [intergenic] C +63 ELD+KKR (N/A) NHEJ 21738127 R
TN0032 tTCCTGCCCAGCTCCATttccttctcctggaactACCAGAACAACACTGAa
aAGGACGGGTCGAGGTAaaggaagaggaccttgaTGGTCTTGTTGTGACTt
    16+[17]+16
rat, IGG2B G (std.) +63 ELD+KKR (N/A) NHEJ 21822240 R
TN0032-T002 tTCCTGACCAGCTCCAAgcataaaagcatAACAGTAGAAAATTGAt
aAGGACTGGTCGAGGTTcgtattttcgtaTTGTCATCTTTTAACTa
    16+[12]+16
rat, [intergenic] +63 ELD+KKR (N/A) NHEJ 21822240 R
TN0150 tACAATACTCCCACTGAAatgacagatgcagacagACTCATGGACAGTTGGTa
aTGTTATGAGGGTGACTTtactgtctacgtctgtcTGAGTACCTGTCAACCAt
    17+[17]+17
zebrafish, epas1b G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0149 tCAGAGCGGCGTAAGGAGaaatctcgtgatgccgCGCGCTGCAGACGGAGTa
aGTCTCGCCGCATTCCTCtttagagcactacggcGCGCGACGTCTGCCTCAt
    17+[16]+17
zebrafish, epas1b G C (std.) +63 WT; ELD+KKR FLASH (Inactive) 22684503 R No
TN0148 tCCGGCAGACATCGTGAAggtggccatcgagtggCCTGGGGCCTTCCCCAAa
aGGCCGTCTGTAGCACTTccaccggtagctcaccGGACCCCGGAAGGGGTTt
    17+[16]+17
zebrafish, elmo1 G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0150-T002 tACGCTACTCCCACTGAAatgacagatgcagacagACTCATGGACAGTTGGTa
aTGCGATGAGGGTGACTTtactgtctacgtctgtcTGAGTACCTGTCAACCAt
    17+[17]+17
zebrafish, epas1b [mutated] G C +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0151 tTTGATGGATCTCTGAGCggcccgcagatctgacAAACTCGCGCTGAAGCGa
aAACTACCTAGAGACTCGccgggcgtctagactgTTTGAGCGCGACTTCGCt
    17+[16]+17
zebrafish, fh G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0152 tACTGGAGTTGTCACTGAaaagaaaaggtagggaTTCCCGTTTTTGCTTGGa
aTGACCTCAACAGTGACTtttcttttccatccctAAGGGCAAAAACGAACCt
    17+[16]+17
zebrafish, hif1ab, exon-intron G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0153 tCGGAGCGCAGGAAGGAGaagtccagggatgcagCGCGATCTCGCAGGGGAa
aGCCTCGCGTCCTTCCTCttcaggtccctacgtcGCGCTAGAGCGTCCCCTt
    17+[16]+17
zebrafish, hif1ab G C (std.) +63 WT; ELD+KKR FLASH (Inactive) 22684503 R No
TN0155 tGGTCCAAAATGAAAAAGtaagaggagtaattacCATGAATGAAGAATATGa
aCCAGGTTTTACTTTTTCattctcctcattaatgGTACTTACTTCTTATACt
    17+[16]+17
zebrafish, ptpmt1 G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0157 tAGAAGAGCGGCATCCCGgcgatcaccatgaagaAGAGATACGGCACCAGGa
aTCTTCTCGCCGTAGGGCcgctagtggtacttctTCTCTATGCCGTGGTCCt
    17+[16]+17
zebrafish, slc6a3 G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0116 cCCTCAAgCAGGCCCCGCTGgtgcactGAAGAGcCACCCTGTGg
gGGAGTTcGTCCGGGGCGACcacgtgaCTTCTCgGTGGGACACc
    (4F+1+2F)+[7]+(2F+1+3F)
human, CLTA G C ZP10703 ZP10704 LRGS ELD+KKR Sangamo HR 21297641 R
ZN0117 gGGGGGCCTCaCGCACCcgcggcGCAGGAGCTTCAGTGg
cCCCCCGGAGtGCGTGGgcgccgCGTCCTCGAAGTCACc
    (2F+1+3F)+[6]+5F
human, DNM2 G C ZP10705 ZP10706 LRGS ELD+KKR Sangamo HR 21297641 R
ZN0116B cCCTCAAgCAGGCCCCGCTGgtgcactGAAGAGcCACCCTGTGg
gGGAGTTcGTCCGGGGCGACcacgtgaCTTCTCgGTGGGACACc
    (4F+1+2F)+[7]+(2F+1+3F)
monkey, CLTA G ZP10703 ZP10704 LRGS ELD+KKR Sangamo HR 21297641 R
ZN0122 gGACTCCTTCaccaaggGGAGGGGGAg
cCTGAGGAAGtggttccCCTCCCCCTc
    3F+[7]+3F
human, IDUA, intron-exon G C ZP10718 ZP10719 TGPNRGVTK ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0123 gGCCCCCCCCcgccccGCAGATGAGg
cCGGGGGGGGgcggggCGTCTACTCc
    3F+[6]+3F
human, IDUA, intron-exon G C ZP10720 ZP10721 TGGS / LGGS / LRGS ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0124 aCCCTGCGCCccgcgctGCGGCTGCCg
tGGGACGCGGggcgcgaCGCCGACGGc
    3F+[7]+3F
human, IDUA G C ZP10722 ZP10723 TGPNRGVTK ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0125 gCCCCGCCCCttacccGCCGGCGGCc
cGGGGCGGGGaatgggCGGCCGCCGg
    3F+[6]+3F
human, IDUA G C ZP10724 ZP10725 TGGS / LGGS / LRGS ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0126 gCTCAACCTCgcctatGTGGGCGCCg
cGAGTTGGAGcggataCACCCGCGGc
    3F+[6]+3F
human, IDUA G C ZP10726 ZP10727 TGGS / LGGS / LRGS ELD+KKR CoDA NHEJ-ODC; HR 21663559 R
ZN0127 cAACCTCGCCtatgtgGGCGCCGTCc
gTTGGAGCGGatacacCCGCGGCAGg
    3F+[6]+3F
human, IDUA G C ZP10728 ZP10729 TGGS / LGGS / LRGS ELD+KKR CoDA HR 21663559 R Low
ZN0133 cAGCCTGATGgAACTTAtaagggAGAAAGtGATTTGCAGt
gTCGGACTACcTTGAATattcccTCTTTCaCTAAACGTCa
    (2F+1+3F)+[6]+(2F+1+3F)
worm, ben-1 G ZP10743 ZP10744 (N/A) WT, ELD+KKR(?) Sangamo NHEJ 21700836 R
ZN0138 gGGAGTGgTGCATGGTGtggcaaCAGGTAaGCTCCATTGTGCtt
cCCTCACcACGTACCACaccgttGTCCATtCGAGGTAACACGaa
    (3F+1+2F)+[6]+(2F+1+4F)
human, SNCA G C ZP10755, ZP10756 ZP10757, ZP10758; ZP10759 LRGS WT, ELD+KKR Sangamo NHEJ; HR 21757228 R
ZN0145 cCGTTACTGCcctgtGGGGCAAGGt
gGCAATGACGggacaCCCCGTTCCa
    3F+[5]+3F
human, HBB G C ZP10766; ZP10767 (N/A) EL+KK, ELD+KKR OPEN / CoDA NHEJ; HR 21898685 R
ZN0146 aCCTGACTCCtgtggaGAAGTCTGCc
tGGACTGAGGacacctCTTCAGACGg
    3F+[6]+3F
human, HBB G C ZP10070, ZP10771 (N/A) EL+KK, ELD+KKR OPEN / CoDA NHEJ; HR 21898685 R
ZN0086B cTGCAACTCCAGTCTTTCTagaagaTGGGCGGGAGTCt
gACGTTGAGGTCAGAAAGAtcttctACCCGCCCTCAGa
    6F+[6]+4F
mouse, G ZP10852 ZP10853 TGAAARA(-Q) WT, Sharkey-ELD+KKR MA NHEJ; HR 22169954 R
ZN0202 gAGCATCACCacatcGAGGACGAGc
cTCGTAGTGGtgtagCTCCTGCTCg
    3F+[5]+3F
zebrafish, myod1 G C ZP10886 ZP10887 (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZNU012 (N/A)
    
zebrafish, cdh15 G C (N/A) (N/A) (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZNU013 (N/A)
    
zebrafish, yap G C (N/A) (N/A) (N/A) ELD+KKR CoDA NHEJ 22655075 R
ZNU015 (N/A)
    
zebrafish, tp53 G C (N/A) (N/A) (N/A) ELD+KKR CoDA NHEJ 22655075 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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