EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN with FokI variants EL+KK: 141 ZFN, 2 TALEN records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0206 tGGATCCACATGATCCTGTcatcagtcggttagccACACAGCTCTTTAAAAAAa
aCCTAGGTGTACTAGGACAgtagtcagccaatcggTGTGTCGAGAAATTTTTTt
    18+[16]+18
silk worm, BmBLOS2 G (std.) +63 EL+KK GG-PCR NHEJ, NHEJ-DisDel 23028749 R
TN0207 tCACCAAGTACGCAGATCTaaggagcctagccgcgAACCTGAACAAGACCCTTa
aGTGGTTCATGCGTCTAGAttcctcggatcggcgcTTGGACTTGTTCTGGGAAt
    18+[16]+18
silk worm, BmBLOS2 G (std.) +63 EL+KK GG-PCR NHEJ, NHEJ-DisDel 23028749 R



ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0013 aCGGAGACCTTCCctggccAATGCTCAGGTACTGg
tGCCTCTGGAAGGgaccggTTACGAGTCCATGACc
    4F+[6]+5F
hamster, Dhfr G ZP10035 ZP10036, ZP10037 LRGS WT, EL+KK Sangamo NHEJ 18359850 R
ZN0014 gGCCGCACGCTGCacagatGCACAGCAGGTTa
cCGGCGTGCGACGtgtctaCGTGTCGTCCAAt
    4F+[6]+4F
zebrafish, slc24a5 G C ZP10039 ZP10038 LRGS EL+KK Sangamo NHEJ 18500334 R
ZN0015 gTTCATCCAGACGgtgtttGTGGACGTGGGCt
cAAGTAGGTCTGCcacaaaCACCTGCACCCGa
    4F+[6]+4F
zebrafish, slc24a5 G C ZP10041 ZP10040, ZP10042 LRGS EL+KK Sangamo NHEJ 18500334 R
ZN0019 gGTCATCCTCATCctgatAAACTGCAAAAGg
cCAGTAGGAGTAGgactaTTTGACGTTTTCc
    4F+[5]+4F
human, CCR5 G C ZP10064 ZP10065 LRGS WT, EL+KK Sangamo NHEJ 18587387 R
ZN0019-T002 gGTCGTCCTCATCttaatAAACTGCAAAAAg
cCAGCAGGAGTAGaattaTTTGACGTTTTTc
    4F+[5]+4F
human, CCR2 G C ZP10064-T002 ZP10065-T002 LRGS EL+KK Sangamo NHEJ 18587387 R
ZN0019-T003 aGTCATCCTCATCtcacgGATGAGGATGCCa
tCAGTAGGAGTAGagtgcCTACTCCTACGGt
    4F+[5]+4F
human, ABLIM2, intron G C ZP10064 ZP10064-T003 LRGS EL+KK Sangamo NHEJ 18587387 R Low
ZN0023 cAGCGTCTTCgagagtGAGGACGTGt
gTCGCAGAAGctctcaCTCCTGCACa
    3F+[6]+3F
human, VEGFA, 5'-up G C ZP10176, ZP10181 ZP10186, ZP10190 LRGS WT, EL+KK OPEN NHEJ; HR 18657511 R
ZN0024 gAGCAGCGTCttcgaGAGTGAGGAc
cTCGTCGCAGaagctCTCACTCCTg
    3F+[5]+3F
human, VEGFA, 5'-up G C ZP10213, ZP10216 ZP10224, ZP10227 LRGS WT, EL+KK OPEN NHEJ; HR 18657511 R
ZN0032 gAGCCCCTCCtattgaGATGAAGCTg
cTCGGGGAGGataactCTACTTCGAc
    3F+[6]+3F
zebrafish, tfr2 G C ZP10376 ZP10380 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0033 gGACCACTTCgctgcGGGGACGTCa
cCTGGTGAAGcgacgCCCCTGCAGt
    3F+[5]+3F
zebrafish, dat G C ZP10346 ZP10353 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0034 cTCCATCTACagtcatGAAGAAGCTg
gAGGTAGATGtcagtaCTTCTTCGAc
    3F+[6]+3F
zebrafish, tert G C ZP10363 ZP10368 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0035 aCCCCTACACctgctGCAGGAGGTg
tGGGGATGTGgacgaCGTCCTCCAc
    3F+[5]+3F
zebrafish, hif1ab G C ZP10358 ZP10359 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0036 aTCCTGCTGCgttcctGCAGCAGAGc
tAGGACGACGcaaggaCGTCGTCTCg
    3F+[6]+3F
zebrafish, hey2 G C ZP10385 ZP10388 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0038 gGGCAGCACCagatgTGGGCTGCTc
cCCGTCGTGGtctacACCCGACGAg
    3F+[5]+3F
tobacco, SuRB G ZP10400 ZP10406 LRGS EL+KK OPEN NHEJ; HR 19404258 R
ZN0038-T002 gGGCAACACCagatgTGGGCTGCTc
cCCGTTGTGGtctacACCCGACGAg
    3F+[5]+3F
tobacco, SuRA G ZP10400-T002 ZP10406 LRGS EL+KK OPEN NHEJ; HR 19404258 R
ZN0039 cCTGTGGGGCCATatcccaGAACTGGTTGAGTCGGTCa
gGACACCCCGGTAtagggtCTTGACCAACTCAGCCAGt
    4F+[6]+6F
maize, ipp2K G ZP10429 ZP10430, ZP10431 LRGS EL+KK Sangamo NHEJ; HR 19404259 R
ZN0039-T002 cCTGTGGGGCCATattccaGAACTGGTTGAGTCTGTCa
gGACACCCCGGTAtaaggtCTTGACCAACTCAGACAGt
    4F+[6]+6F
maize, LOC100126363 G ZP10429 ZP10431-T002 LRGS EL+KK Sangamo NHEJ; HR 19404259 R
ZN0040 cCAACACCTGGGTGCCaatcatGTCGATGGTGGGGTATGGt
gGTTGTGGACCCACGGttagtaCAGCTACCACCCCATACCa
    5F+[6]+6F
maize, ipp2K G ZP10432; ZPU0001 ZP10433; ZPU0002 LRGS EL+KK Sangamo NHEJ; HR 19404259 R
ZN0041 aCCCCTACGCGGAgtacctGAGGCAGCCGCAg
tGGGGATGCGCCTcatggaCTCCGTCGGCGTc
    4F+[6]+4F
maize, Zp15 G ZP10434 ZP10435 LRGS EL+KK Sangamo NHEJ; HR 19404259 R
ZN0042 cGCCCTACTACGCcgggtgTGGGCAGCCGAGc
gCGGGATGATGCGgcccacACCCGTCGGCTCg
    4F+[6]+4F
maize, Zp15 G ZP10436 ZP10437 LRGS EL+KK Sangamo NHEJ; HR 19404259 R
ZN0050 cCCCATCATCTATgcctttGTCGGGGAGAAGt
gGGGTAGTAGATAcggaaaCAGCCCCTCTTCa
    4F+[6]+4F
human, CCR5 G C ZP10452 ZP10453 TGEK WT, D+R, EL+KK MA NHEJ 19470664 R
ZN0050-T002 cCCCATCATCTATgccttCGTTGGGGAGAAGt
gGGGTAGTAGATAcggaaGCAACCCCTCTTCa
    4F+[5]+4F
human, CCR2 G C ZP10452 ZP10453-T002 TGEK WT, D+R, EL+KK MA NHEJ 19470664 R Low
ZN0053 cGAGAGCCCCCTGTCTgatgagAATTTGGTGGCCATGGGCt
gCTCTCGGGGGACAGActactcTTAAACCACCGGTACCCGa
    5F+[6]+6F
rat, IGG2B G ZP10460 ZP10461 LRGS EL+KK Sangamo NHEJ 19628861 R
ZN0054 cGTGCACCAAAACTTCTCCtcccacTACCGGGCCACCATTGGTg
gCACGTGGTTTTGAAGAGGagggtgATGGCCCGGTGGTAACCAc
    6F+[6]+6F
rat, Rab38 G ZP10462 ZP10463 LRGS EL+KK Sangamo NHEJ 19628861 R
ZN0055 tACTGTCCCCTTCtgggctcactatGCTGCCGCCCAGt
aTGACAGGGGAAGacccgagtgataCGACGGCGGGTCa
    4F+[12]+4F
human, CCR5 G C ZP10465 ZP10464 QNKKGLD(G4S)3 WT, D+R, EL+KK MA NHEJ 19635463 R
ZN0056 aGCTCTCATTTTCcatacaGTCAGTATCAATt
tCGAGAGTAAAAGgtatgtCAGTCATAGTTAa
    4F+[6]+4F
human, CCR5 G C ZP10467 ZP10466 QNKK WT, D+R, EL+KK MA NHEJ 19635463 R
ZN0057 cACCCCACAGTGGggccacTAGGGACAGGATt
gTGGGGTGTCACCccggtgATCCCTGTCCTAa
    4F+[6]+4F
human, PPP1R12C, intron G C ZP10468 ZP10469 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0058 cCAGACCtGGCACCCAGgagagGAGCAGGCAGGGTCAGCTg
gGTCTGGaCCGTGGGTCctctcCTCGTCCGTCCCAGTCGAc
    (3F+1+2F)+[5]+6F
human, POU5F1, intron G C ZP10470 ZP10471 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0059 cTGCAGCTGCCCAGACCTGgcaccCAGGAGaGGAGCAGGCa
gACGTCGACGGGTCTGGACcgtggGTCCTCtCCTCGTCCGt
    6F+[5]+(2F+1+3F)
human, POU5F1, intron G C ZP10472 ZP10473 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0060 aGTAGTCCTTTGTTACatgcatGAGTCAGTGAACAGGg
tCATCAGGAAACAATGtacgtaCTCAGTCACTTGTCCc
    5F+[6]+5F
human, POU5F1, intron G C ZP10474 ZP10475 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0061 cCCCAGCTCCCAGAGCacggctGCAAGGGCCaGGAGCAc
gGGGTCGAGGGTCTCGtgccgaCGTTCCCGGtCCTCGTg
    5F+[6]+(3F+1+2F)
human, PITX3 G C ZP10477 ZP10476 LRGS EL+KK Sangamo NHEJ; HR 19680244 R
ZN0061-T002 tAGCTCACaGTCCCTTGT AAAAGGGCCCaGGGAGCAg
aTCGAGTGtCAGGGAACA TTTTCCCGGGtCCCTCGTc
    5F+5F
human, PKP1, intron G C ZP10477-T002 ZP10477-T003 LRGS EL+KK Sangamo NHEJ 19680244 R Low
ZN0062 gCTTGTCTGGATCcaagacCAGGGTGGCTGGg
cGAACAGACCTAGgttctgGTCCCACCGACCc
    4F+[6]+4F
hamster, Bax G ZP10478 ZP10479 LRGS EL+KK Sangamo NHEJ 19777580 R
ZN0063 tGCCAACCAAGGCCTGAAAgatggCGTCTGGACAAGg
aCGGTTGGTTCCGGACTTTctaccGCAGACCTGTTCc
    6F+[5]+4F
hamster, Bak1 G ZP10480 ZP10481 LRGS EL+KK Sangamo NHEJ 19777580 R
ZN0064 cGTCTGCACCtctcaGTGGCGGACc
gCAGACGTGGagagtCACCGCCTGg
    3F+[5]+3F
zebrafish, cxcr4a G C ZP10482 ZP10483 TGGS EL+KK MA & PRS NHEJ 19797767 R
ZN0072 cCCAAGCCCATTCCTGGGAactggaATGGTGCAGGCTg
gGGTTCGGGTAAGGACCCTtgacctTACCACGTCCGAc
    6F+[6]+4F
hamster, Glul G ZPU0003 ZPU0004 LRGS WT, EL+KK Sangamo NHEJ 20047187 R
ZN0073 aCTGATGACCCTTCTTtgttaAAGGAGGCAAAGACAAAGt
tGACTACTGGGAAGAAacaatTTCCTCCGTTTCTGTTTCa
    5F+[5]+6F
hamster, Fut8 G ZPU0007 ZPU0008 LRGS EL+KK Sangamo NHEJ 20047187 R
ZN0074 aCCACCCCCCGGActtgcaTGGGTAGCCGCTg
tGGTGGGGGGCCTgaacgtACCCATCGGCGAc
    4F+[6]+4F
hamster, Dhfr, intron G ZPU0009 ZPU0010 LRGS EL+KK Sangamo NHEJ, NHEJ-DisDel 20047187 R
ZN0075 aCCAACCTCACTATGCactataGGTATGAGAAGGGGGa
tGGTTGGAGTGATACGtgatatCCATACTCTTCCCCCt
    5F+[6]+5F
rat, Il2rg, exon-intron G ZPU0005 ZPU0006 LRGS EL+KK Sangamo NHEJ 20111598 R
ZN0076 aGACATCCAGTTGgctcgCCGGATACGGGGa
tCTGTAGGTCAACcgagcGGCCTATGCCCCt
    4F+[5]+4F
mouse, H3f3b G ZP10498 ZP10499 LRGS EL+KK Sangamo HR 20211137 R
ZNU001 (N/A)
    
human, KDR G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU002 (N/A)
    
human, TP73 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU003 (N/A)
    
human, MAP3K14 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU004 (N/A)
    
human, EP300 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU005 (N/A)
    
human, BTK G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU006 (N/A)
    
human, CARM1 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU007 (N/A)
    
human, GNAI2 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU008 (N/A)
    
human, TSC2 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU009 (N/A)
    
human, RIPK1 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZNU010 (N/A)
    
human, NR3C1 G C (N/A) (N/A) (N/A) EL+KK Sangamo NHEJ 20436476 R
ZN0078 tATCTTCGGCcatgaaGCTGGAGGGt
aTAGAAGCCGgtacttCGACCTCCCa
    3F+[6]+3F
thale cress, ADH1 G ZP10502 ZP10508 LRGS WT, EL+KK OPEN NHEJ 20508152 R
ZN0079 cGGCGTCGACatgcctGGTGCTGACt
gCCGCAGCTGtacggaCCACGACTGa
    3F+[6]+3F
thale cress, TT4 G ZP10511 ZP10525 LRGS WT, EL+KK OPEN NHEJ 20508152 R
ZN0017B aCACACCTTCagcatgTTGGTGGGAc
tGTGTGGAAGtcgtacAACCACCCTg
    3F+[6]+3F
zebrafish, kdrl G C ZP10048 ZP10552 TGGS D+R, EL+KK PRS & MA NHEJ 20843781 R
ZN0017-T002 aTCCCACCCGagtcctGCAGGTGTGt
tAGGGTGGGCtcaggaCGTCCACACa
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10047-T002 ZP10046-T002 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T003 aCACACCATCctacctTTGGTGGGTa
tGTGTGGTAGgatggaAACCACCCAt
    3F+[6]+3F
zebrafish, -, intron G C ZP10046-T003 ZP10047-T003 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T004 aCACACCTTCacagacGTGGGGGGAa
tGTGTGGAAGtgtctgCACCCCCCTt
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10046 ZP10047-T004 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T005 tCCCGACCAGattgtGAAGGTGTGt
aGGGCTGGTCtaacaCTTCCACACa
    3F+[5]+3F
zebrafish, fhdc1 G C ZP10047-T005 ZP10046 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T006 aACCCACCGAgatacGCGGGTGTGt
tTGGGTGGCTctatgCGCCCACACa
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10047-T006 ZP10046-T004 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T007 aCCCACCCTCgtgatgTTGGAGGGAa
tGGGTGGGAGcactacAACCTCCCTt
    3F+[6]+3F
zebrafish, odf3b G C ZP10046-T005 ZP10047-T007 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T008 cCACACCGGCagactGCGGCGGGAt
gGTGTGGCCGtctgaCGCCGCCCTa
    3F+[5]+3F
zebrafish, tmpoa G C ZP10046-T006 ZP10047-T008 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T009 cCACACCCACaaaagATGGTGGGTc
gGTGTGGGTGttttcTACCACCCAg
    3F+[5]+3F
zebrafish, tanc2, intron G C ZP10046-T007 ZP10047-T009 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T010 cTCCCACCCAggaagtGATGGTGAGt
gAGGGTGGGTccttcaCTACCACTCa
    3F+[6]+3F
zebrafish, (intergenic) G C ZP10047-T010 ZP10046-T008 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T011 aTCCCACCGGagcggtGATGGTGAAc
tAGGGTGGCCtcgccaCTACCACTTg
    3F+[6]+3F
zebrafish, - G C ZP10047-T011 ZP10046-T009 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T012 gTCCCGCCAAcaaatGACGGAGTGc
cAGGGCGGTTgtttaCTGCCTCACg
    3F+[5]+3F
zebrafish, -, intron G C ZP10047-T012 ZP10046-T010 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T013 gCGCACCGCCagacatATGGTGGGAt
cGCGTGGCGGtctgtaTACCACCCTa
    3F+[6]+3F
zebrafish, sox6, intron G C ZP10046-T011 ZP10047-T013 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T014 aTCCCCCCTGccatgaGGAGGTGTGt
tAGGGGGGACggtactCCTCCACACa
    3F+[6]+3F
zebrafish, -, intron G C ZP10047-T014 ZP10046-T012 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T015 cACCCACCCActactGAGGGTGAGc
gTGGGTGGGTgatgaCTCCCACTCg
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10047-T015 ZP10046-T013 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T016 tCACACCTCCaattaGAGGCGGGAt
aGTGTGGAGGttaatCTCCGCCCTa
    3F+[5]+3F
zebrafish, (intergenic) G C ZP10046-T012 ZP10047-T016 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T017 gTCCCTCCCTaagggtGATGGGGTGt
cAGGGAGGGAttcccaCTACCCCACa
    3F+[6]+3F
zebrafish, adcyap1b, 3'-UTR G C ZP10047-T017 ZP10046-T014 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T018 gCACACCAGCtgcattTTGGTGGGTc
cGTGTGGTCGacgtaaAACCACCCAg
    3F+[6]+3F
zebrafish, - G C ZP10046-T015 ZP10047-T003 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T019 cTTCCACCAAgtatcaGAAGGTGTAc
gAAGGTGGTTcatagtCTTCCACATg
    3F+[6]+3F
zebrafish, ctnna2 G C ZP10047-T018 ZP10046-T016 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0017-T020 cTCCCACCAGgatatccgggttacGCAGGTGTGg
gAGGGTGGTCctataggcccaatgCGTCCACACc
    3F+[14]+3F
zebrafish, myo5aa G C ZP10047-T019 ZP10046-T002 TGGS D+R, EL+KK PRS NHEJ 20843781 R Low
ZN0093 tTGCTTCATCacaatGGAGATGATt
aACGAAGTAGtgttaCCTCTACTAa
    3F+[5]+3F
soybean, Dcl4a/Dcl4b G ZP10588 ZP10638 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0094 tCTCCACAACatcagGATGACGAAg
aGAGGTGTTGtagtcCTACTGCTTc
    3F+[5]+3F
thale cress, ATMPK8 G ZP10682 ZP10590 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0095 cCTCTTCGTCctatcgGCAGAGGCGc
gGAGAAGCAGgatagcCGTCTCCGCg
    3F+[6]+3F
thale cress, ATMPK11 G ZP10568 ZP10604 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0096 aGCCAGCGACggtggtGGTGGTGGCt
tCGGTCGCTGccaccaCCACCACCGa
    3F+[6]+3F
thale cress, ATMKK9 G ZP10668 ZP10663 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0097 cTTCTTCATCcagatGTTGTTGAGa
gAAGAAGTAGgtctaCAACAACTCt
    3F+[5]+3F
thale cress, MPK15 G ZP10587 ZP10683 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0098 gCCCTTCCACaacaacGGAGAAGCTa
cGGGAAGGTGttgttgCCTCTTCGAt
    3F+[6]+3F
thale cress, MAPKKK18 G ZP10672 ZP10634 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0099 gAGCTACGCCgtagccGGAGACGCCa
cTCGATGCGGcatcggCCTCTGCGGt
    3F+[6]+3F
thale cress, GA3OX2 G ZP10652 ZP10636 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0100 tGCCTTCTCCggggcGCAGAAGGTa
aCGGAAGAGGccccgCGTCTTCCAt
    3F+[5]+3F
zebrafish, actn1 G C ZP10635 ZP10602 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0101 gATCTTCTGCtccaggGGTGAAGGTg
cTAGAAGACGaggtccCCACTTCCAc
    3F+[6]+3F
zebrafish, rag2 G C ZP10601 ZP10655 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0102 cAGCCGCAGCtctcgGCTGTAGACt
gTCGGCGTCGagagcCGACATCTGa
    3F+[5]+3F
zebrafish, gad2 G C ZP10625 ZP10629 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0103 tCTCTTCTCCcccagaGCTGTGGAGa
aGAGAAGAGGgggtctCGACACCTCt
    3F+[6]+3F
zebrafish, lmna G C ZP10633 ZP10630 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0104 gCCCCTCAGCccagaTGGGAGGAGa
cGGGGAGTCGggtctACCCTCCTCt
    3F+[5]+3F
zebrafish, apoeb G C ZP10623 ZP10688 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0105 aCACACCTGCacacaGATGCTGCTg
tGTGTGGACGtgtgtCTACGACGAc
    3F+[5]+3F
zebrafish, trpm7 G C ZP10611 ZP10595 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0106 tGGCCACCTCcaccaGCAGCGGGCc
aCCGGTGGAGgtggtCGTCGCCCGg
    3F+[5]+3F
zebrafish, grip1, intron G C ZP10585 ZP10609 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0107 tCCCCTCTCCtcaaaGCAGATGCAt
aGGGGAGAGGagtttCGTCTACGTa
    3F+[5]+3F
zebrafish, pclo G C ZP10637 ZP10606 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0108 tGGCCCCACCaagcctGCTGGAGGAc
aCCGGGGTGGttcggaCGACCTCCTg
    3F+[6]+3F
zebrafish, jak3 G C ZP10662 ZP10627 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0109 tCTCTGCCGCcacctaGAGGATGGTa
aGAGACGGCGgtggatCTCCTACCAt
    3F+[6]+3F
zebrafish, eif2c1 G C ZP10619 ZP10580 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0110 aGCCCACAGCaatggcGGAGCCGCCt
tCGGGTGTCGttaccgCCTCGGCGGa
    3F+[6]+3F
zebrafish, slitrk1 G C ZP10631 ZP10642 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0111 cGCCGACTGCacagaGAGGAAGTCa
gCGGCTGACGtgtctCTCCTTCAGt
    3F+[5]+3F
zebrafish, bmpr2a G C ZP10576 ZP10612 (N/A) EL+KK CoDA NHEJ 21151135 R
ZN0118 cCTTATTgAGCATCCGGATCctatcTATGATCCCAAGGAGAAGg
gGAATAAcTCGTAGGCCTAGgatagATACTAGGGTTCCTCTTCc
    (4F+1+2F)+[5]+6F
frog, nog G ZP10713, ZP10714 ZP10711, ZP10712 LRGS EL+KK Sangamo NHEJ 21471457 R
ZN0119 cATCCCATTCCCGAGAgctgatCCAATGGTTGCAGATt
gTAGGGTAAGGGCTCTcgactaGGTTACCAACGTCTAa
    5F+[6]+5F
pig, PPARG G ZPU0021 ZPU0023 LRGS EL+KK Sangamo NHEJ 21502977 R
ZN0119-T002 cATTCCATTCCCCAGAtataaCCACTGTTTGGAGTTt
gTAAGGTAAGGGGTCTatattGGTGACAAACCTCAAa
    5F+[5]+5F
pig, CASP10, intron G ZPU0021-T002 ZPU0023-T002 LRGS EL+KK Sangamo NHEJ 21502977 R
ZN0119-T003 tATGGCATTGCCGTGAgttgcgCTATAGGTTGCAGATg
aTACCGTAACGGCACTcaacgcGATATCCAACGTCTAc
    5F+[6]+5F
pig, (intergenic) G ZPU0021-T003 ZPU0023-T003 LRGS EL+KK Sangamo NHEJ 21502977 R
ZN0120 tGACCGCTTCTACcccaatGACTTGTGGGTGg
aCTGGCGAAGATGgggttaCTGAACACCCACc
    4F+[6]+4F
human, CXCR4 G C ZP10715 ZP10716 LRGS EL+KK Sangamo NHEJ 21533216 R
ZN0120-T002 cCACCCACAGGTTttaaggGTAGAAGCAGCAa
gGTGGGTGTCCAAaattccCATCTTCGTCGTt
    4F+[6]+4F
human, ADAMTS17, intron G C ZP10715-T002 ZP10716-T002 LRGS EL+KK Sangamo NHEJ 21533216 R Low
ZN0120-T003 cCACCTACTTGTCttgtgtGTTGAAGCAGACa
gGTGGATGAACAGaacacaCAACTTCGTCTGt
    4F+[6]+4F
human, (intergenic) G C ZP10715-T003 ZP10716-T003 LRGS EL+KK Sangamo NHEJ 21533216 R Low
ZN0121 gGTCGTCCTCATCctgatAAACTGCAAAAGg
cCAGCAGGAGTAGgactaTTTGACGTTTTCc
    4F+[5]+4F
macaque, CCR5 G ZP10717 ZP10065 LRGS EL+KK Sangamo NHEJ 21533216 R
ZN0130 tCCCCTGCTTGGCtgggcgCAGTGGCTCATGc
aGGGGACGAACCGacccgcGTCACCGAGTACg
    4F+[6]+4F
human, TP53, intron G C ZP10734 ZP10735 QNKK WT, EL+KK MMA NHEJ; HR 21695267 R
ZN0131 cCACCATCCACTAcaactaCATGTGTAACAGt
gGTGGTAGGTGATgttgatGTACACATTGTCa
    4F+[6]+4F
human, TP53 G C ZP10736 ZP10737 QNKK WT, EL+KK MMA NHEJ; HR 21695267 R
ZN0137 tCGGTGGCTCAGCTACaggcctGGTGGTACAAGGCACa
aGCCACCGAGTCGATGtccggaCCACCATGTTCCGTGt
    5F+[6]+5F
pig, GGTA1 G ZP10753 ZP10754 LRGS EL+KK Sangamo NHEJ 21730124 R
ZN0141 cCACATCCCAacagaGCAGCGGGAg
gGTGTAGGGTtgtctCGTCGCCCTc
    3F+[5]+3F
zebrafish, hey2 G C ZP10760 ZP10761 LRGS EL+KK (N/A) NHEJ 21822241 R
ZN0142 tAGCTTCTCAGTCacgcacGCCTGTGAGTTTc
aTCGAAGAGTCAGtgcgtgCGGACACTCAAAg
    4F+[6]+4F
zebrafish, gria3a G C ZP10763 ZP10762 LRGS EL+KK (N/A) NHEJ 21822241 R
ZN0143 aTACGCCtCGCCCGAGCtgtgcgGTAGGCATTGAGGCAGCCa
tATGCGGaGCGGGCTCGacacgcCATCCGTAACTCCGTCGGt
    (3F+1+2F)+[6]+6F
human, MALAT1, promoter G C ZP10764 ZP10765 LRGS EL+KK Sangamo NHEJ; HR 21844124 R
ZN0145 cCGTTACTGCcctgtGGGGCAAGGt
gGCAATGACGggacaCCCCGTTCCa
    3F+[5]+3F
human, HBB G C ZP10766; ZP10767 (N/A) EL+KK, ELD+KKR OPEN / CoDA NHEJ; HR 21898685 R
ZN0146 aCCTGACTCCtgtggaGAAGTCTGCc
tGGACTGAGGacacctCTTCAGACGg
    3F+[6]+3F
human, HBB G C ZP10070, ZP10771 (N/A) EL+KK, ELD+KKR OPEN / CoDA NHEJ; HR 21898685 R
ZN0150 aCTCATCCACcagctGGAGATGGCa
tGAGTAGGTGgtcgaCCTCTACCGt
    3F+[5]+3F
zebrafish, ago2 G C ZP10774 ZP10773 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0151 aATCCGTGACggcgcaGTAAGGGCCc
tTAGGCACTGccgcgtCATTCCCGGg
    3F+[6]+3F
zebrafish, arhgef1 G C ZP10775 ZP10776 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0152 aCACCTCCTCctgggtGATGGGGTGg
tGTGGAGGAGgacccaCTACCCCACc
    3F+[6]+3F
zebrafish, braf G C ZP10777 ZP10778 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0153 cCTCCTCCCCagagccGTAGTCGTAc
gGAGGAGGGGtctcggCATCAGCATg
    3F+[6]+3F
zebrafish, chd5 G C ZP10780 ZP10781 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0154 aCCCCTGGTCcggaaGTGTGGGTGt
tGGGGACCAGgccttCACACCCACa
    3F+[5]+3F
zebrafish, col18a1 G C ZP10782 ZP10783 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0155 cCTCAACACCgtcccGCAACGGAGc
gGAGTTGTGGcagggCGTTGCCTCg
    3F+[5]+3F
zebrafish, cxcl12b G C ZP10785 ZP10784 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0064B cGTCTGCACCtctcaGTGGCGGACc
gCAGACGTGGagagtCACCGCCTGg
    3F+[5]+3F
zebrafish, cxcr4a G C ZP10482 ZP10483 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0156 aCTCTCCTTCtccccGAGGGGGTCc
tGAGAGGAAGaggggCTCCCCCAGg
    3F+[5]+3F
zebrafish, dab2 G C ZP10786 ZP10787 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0157 tTTCCTCCACaaagaGGCTGGGATg
aAAGGAGGTGtttctCCGACCCTAc
    3F+[5]+3F
zebrafish, dicer1 G C ZP10789 ZP10790 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0158 tGTCCTCCTCatggcgGTCGATGGTt
aCAGGAGGAGtaccgcCAGCTACCAa
    3F+[6]+3F
zebrafish, drosha G C ZP10791 ZP10792 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0159 cCACGCCCAAgagggGCGGCAGCGg
gGTGCGGGTTctcccCGCCGTCGCc
    3F+[5]+3F
zebrafish, efnb2a G C ZP10794 ZP10793 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0160 aCCCCATTCCccctcaGCAAGAGTGg
tGGGGTAAGGgggagtCGTTCTCACc
    3F+[6]+3F
zebrafish, fli1a G C ZP10798 ZP10797 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0161 cCGCCGCCACagcgggGTGGAGGTGg
gGCGGCGGTGtcgcccCACCTCCACc
    3F+[6]+3F
zebrafish, gata2a G C ZP10802 ZP01801 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0162 cCTCCGCCACagaggaGTCGAGGTGg
gGAGGCGGTGtctcctCAGCTCCACc
    3F+[6]+3F
zebrafish, gata3 G C ZP10803 ZP10804 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0163 cTCCCGCACCagtttgGCATTGGGGt
gAGGGCGTGGtcaaacCGTAACCCCa
    3F+[6]+3F
zebrafish, kif1b G C ZP10805 ZP10806 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0164 aCGCCTCATCgtgacgGTGGAGGAAa
tGCGGAGTAGcactgcCACCTCCTTt
    3F+[6]+3F
zebrafish, kif1b G C ZP10807 ZP10789 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0165 aCTCTCCCCAgggagCAGGCAGCAt
tGAGAGGGGTccctcGTCCGTCGTa
    3F+[5]+3F
zebrafish, klf2a G C ZP10809 ZP10808 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0166 aGCCCTCCAAtctgaaGGTGCAGGAg
tCGGGAGGTTagacttCCACGTCCTc
    3F+[6]+3F
zebrafish, micall2 G C ZP10811 ZP10810 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0167 cACCCTCCTCaggctGTAGAGGAAg
gTGGGAGGAGtccgaCATCTCCTTc
    3F+[5]+3F
zebrafish, nf1a G C ZP10813 ZP10814 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0168 tTTCCGTTACctgtgtGAGGAGGCAg
aAAGGCAATGgacacaCTCCTCCGTc
    3F+[6]+3F
zebrafish, nf1b G C ZP10816 ZP10815 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0169 cCGTTACCTGtgtgagGAGGCAGAAa
gGCAATGGACacactcCTCCGTCTTt
    3F+[6]+3F
zebrafish, nf1b G C ZP10818 ZP10817 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0170 cATCCGCCTGccactGGTGTGGTCg
gTAGGCGGACggtgaCCACACCAGc
    3F+[5]+3F
zebrafish, numbl G C ZP10819 ZP10820 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0171 aCACCTCATCtggacGTGGAGGAGt
tGTGGAGTAGacctgCACCTCCTCa
    3F+[5]+3F
zebrafish, ptpru G C ZP10825 ZP10824 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0172 cCTCCTCCTCttcctCGGGTGGTGg
gGAGGAGGAGaaggaGCCCACCACc
    3F+[5]+3F
zebrafish, sbno2 G C ZP10828 ZP10829 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0173 gGACTACATCaatggaGGAGAGGTGt
cCTGATGTAGttacctCCTCTCCACa
    3F+[6]+3F
zebrafish, sgk1 G C ZP10831 ZP10830 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0174 cCTCCGCCCCttatcaCAGGTGGAGg
gGAGGCGGGGaatagtGTCCACCTCc
    3F+[6]+3F
zebrafish, spon1b G C ZP10834 ZP10835 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0175 tTCCCACCACtcagcAAGTGGGACg
aAGGGTGGTGagtcgTTCACCCTGc
    3F+[5]+3F
zebrafish, tie1 G C ZP10837 ZP10836 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0176 gACCCTCGGCcttcagCAGGTCGTAc
cTGGGAGCCGgaagtcGTCCAGCATg
    3F+[6]+3F
zebrafish, tarbp2 G C ZP10838 ZP10839 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0177 cACACACCTGtgagtcCAGGAGGACg
gTGTGTGGACactcagGTCCTCCTGc
    3F+[6]+3F
zebrafish, yrk G C ZP10841 ZP10842 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0185 aACCCACTGCcgtgacGGAGGAGCGt
tTGGGTGACGgcactgCCTCCTCGCa
    3F+[6]+3F
yellow catfish, mstn G ZP10854 ZP10855 (N/A) EL+KK MA NHEJ 22194943 R
ZN0120B tGACCGCTTCTACcccaatGACTTGTGGGTGg
aCTGGCGAAGATGgggttaCTGAACACCCACc
    4F+[6]+4F
mouse, Cxcr4 G ZP10706 ZP10065 LRGS EL+KK Sangamo NHEJ 22273578 R
ZNU014 (N/A)
    
zebrafish, ptenb G C (N/A) (N/A) (N/A) EL+KK CoDA NHEJ 22655075 R
ZN0054-T002 gCACCAAAACTTCTCCtcgcacTACCGGGCCACCATTGTg
cGTGGTTTTGAAGAGGagcgtgATGGCCCGGTGGTAACAc
    6F+[6]+6F
mouse, Rab38 G ZP10462 ZP10463 LRGS EL+KK Sangamo NHEJ 22660928 R
ZN0216 gCGCCCCTGCgcaacGTGGCAGGAa
cGCGGGGACGcgttgCACCGTCCTt
    3F+[5]+3F
mouse, G ZP10916 ZP10915 LRGS WT, EL+KK OPEN NHEJ; HR 22970113 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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