EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN constructed bys OPEN: 30 ZFN, 0 TALEN records match.
(Click on ID to see details)


ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0008B cACCATCTTCttcaagGACGACGGCa
gTGGTAGAAGaagttcCTGCTGCCGt
    3F+[6]+3F
eGFP G ZP10028 ZP10019 (N/A) P509T+N586S, WT, D+R OPEN 18334988 R
ZN0008C cACCATCTTCttcaagGACGACGGCa
gTGGTAGAAGaagttcCTGCTGCCGt
    3F+[6]+3F
eGFP G ZP10031, ZP10032 ZP10034 (N/A) P509T+N586S, WT, D+R OPEN 18334988 R
ZN0002B cTACCCCGACcacatGAAGCAGCAc
gATGGGGCTGgtgtaCTTCGTCGTg
    3F+[5]+3F
eGFP G ZP10066, ZP10070 ZP10073, ZP10077 LRGS WT OPEN NHEJ 18657511 R
ZN0008D cACCATCTTCttcaagGACGACGGCa
gTGGTAGAAGaagttcCTGCTGCCGt
    3F+[6]+3F
eGFP G ZP10089, ZP10092 ZP10096, ZP10099 LRGS WT OPEN NHEJ 18657511 R
ZN0020 gGGCATCGACttcaagGAGGACGGCa
cCCGTAGCTGaagttcCTCCTGCCGt
    3F+[6]+3F
eGFP G ZP10104, ZP10105 ZP10111, ZP10112 LRGS WT OPEN NHEJ 18657511 R
ZN0021 gATCCGCCACaacatcGAGGACGGCa
cTAGGCGGTGttgtagCTCCTGCCGt
    3F+[6]+3F
eGFP G ZP10120, ZP10121 ZP10128, ZP10134 LRGS WT OPEN NHEJ 18657511 R
ZN0022 tCACTTCTAAtggtgaTTATGGGAGa
aGTGAAGATTaccactAATACCCTCt
    3F+[6]+3F
human, CFTR, intron G C ZP10153 ZP10165 LRGS WT OPEN NHEJ 18657511 R
ZN0023 cAGCGTCTTCgagagtGAGGACGTGt
gTCGCAGAAGctctcaCTCCTGCACa
    3F+[6]+3F
human, VEGFA, 5'-up G C ZP10176, ZP10181 ZP10186, ZP10190 LRGS WT, EL+KK OPEN NHEJ; HR 18657511 R
ZN0024 gAGCAGCGTCttcgaGAGTGAGGAc
cTCGTCGCAGaagctCTCACTCCTg
    3F+[5]+3F
human, VEGFA, 5'-up G C ZP10213, ZP10216 ZP10224, ZP10227 LRGS WT, EL+KK OPEN NHEJ; HR 18657511 R
ZN0025 cCCCAGCCGCcccactGAGTTTGCCt
gGGGTCGGCGgggtgaCTCAAACGGa
    3F+[6]+3F
human, HOXB13 G C ZP10280, ZP10282 ZP10284, ZP10285 LRGS WT OPEN NHEJ 18657511 R
ZN0026 aTGCCACCCAtgcccTGGGGTGCCc
tACGGTGGGTacgggACCCCACGGg
    3F+[5]+3F
human, HOXB13 G C ZP10297, ZP10299 ZP10302, ZP10306 LRGS WT OPEN NHEJ 18657511 R
ZN0027 gAGACTCCCAcggccGGGGAAGAGt
cTCTGAGGGTgccggCCCCTTCTCa
    3F+[5]+3F
human, HOXB13 G C ZP10314, ZP10319 ZP10325, ZP10326 LRGS WT OPEN NHEJ 18657511 R
ZN0028 gGAATCCTTCtaatgaGGCGGAGATc
cCTTAGGAAGattactCCGCCTCTAg
    3F+[6]+3F
tobacco, SuRB/SuRA G ZP10335 ZP10337 LRGS WT OPEN NHEJ 18657511 R
ZN0032 gAGCCCCTCCtattgaGATGAAGCTg
cTCGGGGAGGataactCTACTTCGAc
    3F+[6]+3F
zebrafish, tfr2 G C ZP10376 ZP10380 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0033 gGACCACTTCgctgcGGGGACGTCa
cCTGGTGAAGcgacgCCCCTGCAGt
    3F+[5]+3F
zebrafish, dat G C ZP10346 ZP10353 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0034 cTCCATCTACagtcatGAAGAAGCTg
gAGGTAGATGtcagtaCTTCTTCGAc
    3F+[6]+3F
zebrafish, tert G C ZP10363 ZP10368 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0035 aCCCCTACACctgctGCAGGAGGTg
tGGGGATGTGgacgaCGTCCTCCAc
    3F+[5]+3F
zebrafish, hif1ab G C ZP10358 ZP10359 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0036 aTCCTGCTGCgttcctGCAGCAGAGc
tAGGACGACGcaaggaCGTCGTCTCg
    3F+[6]+3F
zebrafish, hey2 G C ZP10385 ZP10388 LRGS EL+KK OPEN NHEJ 19198653 R
ZN0038 gGGCAGCACCagatgTGGGCTGCTc
cCCGTCGTGGtctacACCCGACGAg
    3F+[5]+3F
tobacco, SuRB G ZP10400 ZP10406 LRGS EL+KK OPEN NHEJ; HR 19404258 R
ZN0038-T002 gGGCAACACCagatgTGGGCTGCTc
cCCGTTGTGGtctacACCCGACGAg
    3F+[5]+3F
tobacco, SuRA G ZP10400-T002 ZP10406 LRGS EL+KK OPEN NHEJ; HR 19404258 R
ZN0051 aGGCTACATCtttgcTTTGTTGGCa
tCCGATGTAGaaacgAAACAACCGt
    3F+[5]+3F
human, PIGA G C ZP10454; ZP10455 ZP10457; ZP10456 LRGS WT OPEN NHEJ; HR 19540188 R
ZN0078 tATCTTCGGCcatgaaGCTGGAGGGt
aTAGAAGCCGgtacttCGACCTCCCa
    3F+[6]+3F
thale cress, ADH1 G ZP10502 ZP10508 LRGS WT, EL+KK OPEN NHEJ 20508152 R
ZN0079 cGGCGTCGACatgcctGGTGCTGACt
gCCGCAGCTGtacggaCCACGACTGa
    3F+[6]+3F
thale cress, TT4 G ZP10511 ZP10525 LRGS WT, EL+KK OPEN NHEJ 20508152 R
ZN0145 cCGTTACTGCcctgtGGGGCAAGGt
gGCAATGACGggacaCCCCGTTCCa
    3F+[5]+3F
human, HBB G C ZP10766; ZP10767 (N/A) EL+KK, ELD+KKR OPEN / CoDA NHEJ; HR 21898685 R
ZN0146 aCCTGACTCCtgtggaGAAGTCTGCc
tGGACTGAGGacacctCTTCAGACGg
    3F+[6]+3F
human, HBB G C ZP10070, ZP10771 (N/A) EL+KK, ELD+KKR OPEN / CoDA NHEJ; HR 21898685 R
ZN0182 aGCCGCCCACgtccatGTTTGAGGAt
tCGGCGGGTGcaggtaCAAACTCCTa
    3F+[6]+3F
zebrafish, kiaa0586 G C ZP10849 ZP10850 LRGS D+R OPEN NHEJ 22028029 R
ZN0211 cTCCTACAGCaaccagGCAGTGGCCt
gAGGATGTCGttggtcCGTCACCGGa
    3F+[6]+3F
zebrafish, foxp2 G C ZPU0067 ZPU0068 (N/A) D+R OPEN NHEJ 22937139 R
ZN0216 gCGCCCCTGCgcaacGTGGCAGGAa
cGCGGGGACGcgttgCACCGTCCTt
    3F+[5]+3F
mouse, G ZP10916 ZP10915 LRGS WT, EL+KK OPEN NHEJ; HR 22970113 R
ZN0219 cACCTCCTCCccggccGGCGGCGGAc
gTGGAGGAGGggccggCCGCCGCCTg
    3F+[6]+3F
human, VEGFA G C ZP10917 - ZP10920 ZP10920 - ZP10923 LRGS D+R OPEN HR 23028823 R
ZN0220 aGTCACCTTCcctgagTGGGCTGGTa
tCAGTGGAAGggactcACCCGACCAt
    3F+[6]+3F
human, GRN, intron G C ZP10924 - ZP10925 ZP10928 - ZP10934 LRGS D+R OPEN HR 23028823 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
GSearch EENdb with this gene symbol
RSearch EENdb with this reference

©2012-2013  PKU Zebrafish Functional Genomics Group
School of Life Sciences, Peking University, Beijing, China
Reference of EENdb    Contact us