EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN constructed bys MA: 133 ZFN, 0 TALEN records match.
(Click on ID to see details)


ZFN IDWhole SiteSpecies & LocusZFP Domain L.ZFP Domain R.LinkerFokIConstruc.Modif.Ref.Effic.
ZN0001 tGCCTACCGCattaaaGTGGATGAGt
aCGGATGGCGtaatttCACCTACTCa
    3F+[6]+3F
fruitfly, y G ZP10002 ZP10001 QNKK WT MA NHEJ 12136019 R
ZN0002 cCGCTACCCCgaccacatGAAGCAGCAc
gGCGATGGGGctggtgtaCTTCGTCGTg
    3F+[8]+3F
eGFP G ZP10003 ZP10004 (N/A) WT MA HR 15806097 R
ZNA006 nACCATCTTCgaatttAGGGATAACn
nTGGTAGAAGcttaaaTCCCTATTGn
    3F+[6]+3F
(artificial) ZP00106 ZP00107 AAARA+(0/16aa) WT MA 16039907 R
ZNA006B nACCATCTTCgaatttGAAGATGGTn
nTGGTAGAAGcttaaaCTTCTACCAn
    3F+[6]+3F
(artificial) ZP00106 ZP00106 AAARA+(0/16aa) WT MA 16039907 R
ZNA006C nGTTATCCCTgaatttAGGGATAACn
nCAATAGGGActtaaaTCCCTATTGn
    3F+[6]+3F
(artificial) ZP00107 ZP00107 AAARA+(0/16aa) WT MA 16039907 R
ZN0004 cTGACTCCTGatgttgattTTGGGAGAAc
gACTGAGGACtacaactaaAACCCTCTTg
    3F+[9]+3F
mouse, Cftr G ZP10008 ZP10007 TG(G4S)3 WT MA 16084494 R
ZN0005 gTTCCCCTTCaaagggGTGGATGACc
cAAGGGGAAGtttcccCACCTACTGg
    3F+[6]+3F
mouse, Tyr G ZP10010 ZP10009 TG WT MA 16084494 R
ZN0006 tGTCCCCTTCtgggctcactatGCTGCCGCCc
aCAGGGGAAGacccgagtgataCGACGGCGGg
    3F+[12]+3F
human, CCR5 G C ZP10013 ZP10014 TG(G4S)3 WT MA 16084494 R
ZN0007 gGCCCGCCCCctagcgGCCGGGGAGg
cCGGGCGGGGgatcgcCGGCCCCTCc
    3F+[6]+3F
human, DMPK, 3'-UTR G C ZP10012 ZP10011 TG WT MA 16084494 R
ZNA007 nAGCAACCTCnnnnnnGCGTGGGCGn
nTCGTTGGAGnnnnnnCGCACCCGCn
    3F+[6]+3F
(artificial) ZP10015 ZP00002 (3aa)+QNKK WT MA+Natural 16169774 R
ZNA008 nTGCTCCCCCnnnnnnGCGTGGGCGn
nACGAGGGGGnnnnnnCGCACCCGCn
    3F+[6]+3F
(artificial) ZP10016, ZP10017 ZP00002 (3aa)+QNKK WT MA+Natural 16169774 R
ZN0008 cACCATCTTCttcaagGACGACGGCa
gTGGTAGAAGaagttcCTGCTGCCGt
    3F+[6]+3F
eGFP G ZP10018 ZP10019 (3aa) WT MA HR 16169774 R
ZN0009 cGGCGCCCACcatcgaGTCGCAGCCc
gCCGCGGGTGgtagctCAGCGTCGGg
    3F+[6]+3F
human, CD8A G C ZP10020 ZP10021 (3aa) WT MA HR 16169774 R
ZNA009 nCGCCCACGCatgccaGCGTGGGCGn
nGCGGGTGCGtacggtCGCACCCGCn
    3F+[6]+3F
(artificial) ZP00002 ZP00002 (3aa)+QNKK WT MA 16262717 R
ZN0010 tAGCTACTACacgaatGGCGTGGGAg
aTCGATGATGtgcttaCCGCACCCTc
    3F+[6]+3F
fruitfly, ry G ZP10025 ZP10024 QNKK WT MA NHEJ; HR 16452139 R
ZN0011 tCCCATCATCaggcggGAGGTGGGCg
aGGGTAGTAGtccgccCTCCACCCGc
    3F+[6]+3F
fruitfly, bw G ZP10022 ZP10023 QNKK WT MA NHEJ 16452139 R
ZN0012 aACCCACTCCaagcttGGGGTGGCTt
tTGGGTGAGGttcgaaCCCCACCGAa
    3F+[6]+3F
worm, (intergenic) G ZP10027 ZP10026 QNKK WT MA NHEJ 17060623 R
ZNA004F nTTCTTCCCCacgcgtGGGGAAGAAn
nAAGAAGGGGtgcgcaCCCCTTCTTn
    3F+[6]+3F
worm ZP10027 ZP10026 QNKK WT MA 17060623 R
ZNA010 aCACTGCGGCtttaaaGCCGCAGTGc
tGTGACGCCGaaatttCGGCGTCACg
    3F+[6]+3F
(artificial) ZP00113; ZP00114; ZP00115 ZP00113; ZP00114; ZP00115 AAARA(5aa?) WT MA 18026168 R
ZNA011 aTGCAGCATCtttaaaGATGCTGCAc
tACGTCGTAGaaatttCTACGACGTg
    3F+[6]+3F
(artificial) ZP00116; ZP00117 ZP00116; ZP00117 AAARA(5aa?) WT MA 18026168 R
ZNA012 aGGCTTCTGCtttaaaGCAGAAGCCc
tCCGAAGACGaaatttCGTCTTCGGg
    3F+[6]+3F
(artificial) ZP00118; ZP00119; ZP00120 ZP00118; ZP00119; ZP00120 AAARA(5aa?) WT MA 18026168 R
ZN0018 cCTACTCaTTCAACcaataGCCCGGGCCGTAc
gGATGAGtAAGTTGgttatCGGGCCCGGCATg
    (2F+1+2F)+[5]+4F
human, (mtDNA) G C ZP10051, ZP10052; ZP10053 - ZP10057 ZP10061, ZP10062; ZP10063 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018-T002 cCTACTCaTTCAACcaataGCCCTGGCCGTAc
gGATGAGtAAGTTGgttatCGGGACCGGCATg
    (2F+1+2F)+[5]+4F
human, (mtDNA) G C ZP10053 - ZP10057 ZP10061-T002 - ZP10063-T002 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018B cAGCCTACTCaTTCAACcaataGCCCGGGCCGTAc
gTCGGATGAGtAAGTTGgttatCGGGCCCGGCATg
    (2F+1+3F)+[5]+4F
human, (mtDNA) G C ZP10058 ZP10061 - ZP10063 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018B-T002 cAGCCTACTCaTTCAACcaataGCCCTGGCCGTAc
gTCGGATGAGtAAGTTGgttatCGGGACCGGCATg
    (2F+1+3F)+[5]+4F
human, (mtDNA) G C ZP10058 ZP10061-T002 - ZP10063-T002 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZN0018C
(Z-S1)
GCCCGGGCCGTAc
CGGGCCCGGCATg
    4F
human, (mtDNA) [mutated] G C ZP10063 S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZNU017
(Z-S1)
(N/A)
    
human, (mtDNA) G C S(G4S)3S(?) WT MA(2F) NHEJ 18511461 R
ZNA014 nTGCATCCATnnnnnnATGGATGCAn
nACGTAGGTAnnnnnnTACCTACGTn
    3F+[6]+3F
(artificial) ZP00121 ZP00121 (N/A) WT MA 18725642 R
ZNA015 nTTCCCACAAnnnnnnTTGTGGGAAn
nAAGGGTGTTnnnnnnAACACCCTTn
    3F+[6]+3F
(artificial) ZP00122 ZP00122 (N/A) WT MA 18725642 R
ZNA016 cAGCACCAACaccaGCTGCTGTCt
gTCGTGGTTGtggtCGACGACAGa
    3F+[4]+3F
(artificial) ZP00123 ZP00124 (N/A) WT MA 18725642 R
ZNA017
(Z-S2)
GGTCGGGACcatatgtatacatatgtatacGTTGCGGGAt
CCAGCCCTGgtatacatatgtatacatatgCAACGCCCTa
    3F+[21]+3F
(artificial) ZP00125 ZP00097 TGEK(G4S)3 Snase MA 18980382 R
ZNA017B
(Z-M)
GTTGCGGGAt
CAACGCCCTa
    3F
(artificial) ZP00097 TGEK(G4S)3 Snase MA 18980382 R Low
ZNA017C
(Z-M)
GGTCGGGACc
CCAGCCCTGg
    3F
(artificial) ZP00125 TGEK(G4S)3 Snase MA 18980382 R Low
ZNA017D
(Z-M)
(target sequence error)
(please contact us)

    6F
(artificial) ZP00126 TGEK(G4S)3 Snase MA 18980382 R Low
ZN0029 aCACTCCAGCatgaagGTGGATCTAt
tGTGAGGTCGtacttcCACCTAGATa
    3F+[6]+3F
fruitfly, coil G ZP10340 ZP10339 QNKK WT MA NHEJ 19064913 R
ZN0030 gAGCCACATCtcctccCTGGCGGAGa
cTCGGTGTAGaggaggGACCGCCTCt
    3F+[6]+3F
fruitfly, pask G ZP10342 ZP10341 QNKK WT MA NHEJ 19064913 R
ZN0037 cTACCACGTCacgagcAGGGTGGTGt
gATGGTGCAGtgctcgTCCCACCACa
    3F+[6]+3F
tobacco, SuRB G ZP10392 ZP10393 LRGS WT, D+R MA NHEJ; HR 19404258 R
ZN0037-T002 cTGCCACGTCacgagcAGGGCGGTGt
gACGGTGCAGtgctcgTCCCGCCACa
    3F+[6]+3F
tobacco, SuRA G ZP10392-T002 ZP10393-T002 LRGS WT, D+R MA NHEJ; HR 19404258 R
ZNA020
(Z-S1)
GAAAACGGTCGGGACCGAa
CTTTTGCCAGCCCTGGCTt
    6F
(artificial) ZP00108 TGEK(G4S)3 WT MA 19428709 R
ZN0043 aAGTCCAATCTATgacatcAATTATTATACAt
tTCAGGTTAGATActgtagTTAATAATATGTa
    4F+[6]+4F
human, CCR5 G C ZP10438 ZP10439 TGEK WT MA NHEJ 19470664 R
ZN0044 tCTGGGCTCACTAtgctgcCGCCCAGTGGGAc
aGACCCGAGTGATacgacgGCGGGTCACCCTg
    4F+[6]+4F
human, CCR5 G C ZP10440 ZP10441 TGEK WT MA NHEJ 19470664 R
ZN0045 cTTCTTCATCATCctcctGACAATCGAt
gAAGAAGTAGTAGgaggaCTGTTAGCTa
    4F+[5]+3F
human, CCR5/CCR2 G C ZP10442 ZP10443 TGEK WT MA NHEJ 19470664 R
ZN0046 cTGTGTTTGCTTTaaaagcCAGGACGGTCACc
gACACAAACGAAAttttcgGTCCTGCCAGTGg
    4F+[6]+4F
human, CCR5/CCR2 G C ZP10444 ZP10445 TGEK WT MA NHEJ 19470664 R
ZN0047 cAGGACGGTCacctttGGGGTGGTGa
gTCCTGCCAGtggaaaCCCCACCACt
    3F+[6]+3F
human, CCR5 G C ZP10446 ZP10447 TGEK WT MA NHEJ 19470664 R
ZN0048 aCGGTCACCTttggggTGGTGACAAGTGt
tGCCAGTGGAaaccccACCACTGTTCACa
    3F+[6]+4F
human, CCR5/CCR2 G C ZP10448 ZP10449 TGEK WT MA NHEJ 19470664 R
ZN0049 aCCAAGCTATgcaggtGACAGAGACt
tGGTTCGATAcgtccaCTGTCTCTGa
    3F+[6]+3F
human, CCR5 G C ZP10450 ZP10451 TGEK WT MA NHEJ 19470664 R
ZN0050 cCCCATCATCTATgcctttGTCGGGGAGAAGt
gGGGTAGTAGATAcggaaaCAGCCCCTCTTCa
    4F+[6]+4F
human, CCR5 G C ZP10452 ZP10453 TGEK WT, D+R, EL+KK MA NHEJ 19470664 R
ZN0050-T002 cCCCATCATCTATgccttCGTTGGGGAGAAGt
gGGGTAGTAGATAcggaaGCAACCCCTCTTCa
    4F+[5]+4F
human, CCR2 G C ZP10452 ZP10453-T002 TGEK WT, D+R, EL+KK MA NHEJ 19470664 R Low
ZN0055 tACTGTCCCCTTCtgggctcactatGCTGCCGCCCAGt
aTGACAGGGGAAGacccgagtgataCGACGGCGGGTCa
    4F+[12]+4F
human, CCR5 G C ZP10465 ZP10464 QNKKGLD(G4S)3 WT, D+R, EL+KK MA NHEJ 19635463 R
ZN0056 aGCTCTCATTTTCcatacaGTCAGTATCAATt
tCGAGAGTAAAAGgtatgtCAGTCATAGTTAa
    4F+[6]+4F
human, CCR5 G C ZP10467 ZP10466 QNKK WT, D+R, EL+KK MA NHEJ 19635463 R
ZN0064 cGTCTGCACCtctcaGTGGCGGACc
gCAGACGTGGagagtCACCGCCTGg
    3F+[5]+3F
zebrafish, cxcr4a G C ZP10482 ZP10483 TGGS EL+KK MA & PRS NHEJ 19797767 R
ZNA017E
(Z-S2)
nGGTCGGGACcn(x)GTTGCGGGAt
nCCAGCCCTGgn(x)CAACGCCCTa
    3F+[x]+3F
(artificial) ZP00125 ZP00097 TGEK(G4S)3 WT MA 19825368 R
ZN0065 aGGCTCCTCCTCCaacattGGGGATTATGATt
tCCGAGGAGGAGGttgtaaCCCCTAATACTAa
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10484 ZP10485 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0066 cTCATCCATTTGCactcctGGGTATGGAGACc
gAGTAGGTAAACGtgaggaCCCATACCTCTGg
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10486 ZP10487 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0067 cATACCCACCTCAcgtgtgGTCAATGGAGTGt
gTATGGGTGGAGTgcacacCAGTTACCTCACa
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10488 ZP10489 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0068 gGTCCCATCACCAgcgatgGATATAGTCTGGa
cCAGGGTAGTGGTcgctacCTATATCAGACCt
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10490 ZP10491 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0069 gGTCTGCAGCCACagctggGTGGATGCAGCTg
cCAGACGTCGGTGtcgaccCACCTACGTCGAc
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10492 ZP10493 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0070 tGACCTCACCACCtctcagTGGGAAGAGGAGg
aCTGGAGTGGTGGagagtcACCCTTCTCCTCc
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10494 ZP10495 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0071 tTTCTATGGCAGCctcaggGGAGAATGGGACt
aAAGATACCGTCGgagtccCCTCTTACCCTGa
    4F+[6]+4F
human, CCR2/CCR5, 5'-up G C ZP10496 ZP10497 TGEK WT MA NHEJ-DisDel 19952142 R
ZN0077 cCGCCGCCACcgtaGGAGGAGGAg
gGCGGCGGTGgcatCCTCCTCCTc
    3F+[4]+3F
thale cress, ATABI4 G ZP10501 ZP10500 LRGS D+R MA NHEJ 20508151 R
ZN0084 aGGCCGCTGCccagcaGCAGGAGTCa
tCCGGCGACGggtcgtCGTCCTCAGt
    3F+[6]+3F
human, RHO G C ZP10543 ZP10542 (G3S)4 WT MA NHEJ; HR 20671268 R
ZN0085 tGCTGCTGCTgctgctgctGCTGCTGCTg
aCGACGACGAcgacgacgaCGACGACGAc
    3F+[3n]+3F
human, (microsatellite) G C ZP10545 ZP10544 (N/A) WT MA HR 20676085 R
ZN0087 cGCGATCACCaagtacGCAGATCTAa
gCGCTAGTGGttcatgCGTCTAGATt
    3F+[6]+3F
silk worm, BmBLOS2 G ZP10547 ZP10546 TGQK WT MA NHEJ 20692340 R
ZN0088 gAGTCTCAGCacagaaGACATAGAAa
cTCAGAGTCGtgtcttCTGTATCTTt
    3F+[6]+3F
silk worm, BmBLOS2 G ZP10549 ZP10548 TGQK WT MA NHEJ 20692340 R
ZN0089 aGTTACTTACacgtggGCCGATGTCa
tCAATGAATGtgcaccCGGCTACAGt
    3F+[6]+3F
silk worm, Bmwh3 G ZP10551 ZP10550 TGQK WT MA NHEJ 20692340 R
ZN0017B aCACACCTTCagcatgTTGGTGGGAc
tGTGTGGAAGtcgtacAACCACCCTg
    3F+[6]+3F
zebrafish, kdrl G C ZP10048 ZP10552 TGGS D+R, EL+KK PRS & MA NHEJ 20843781 R
ZNA014-T002 cAGCATCCATgtaagtATGGATGCTg
gTCGTAGGTAcattcaTACCTACGAc
    3F+[6]+3F
(artificial) ZP00121-T002 ZP00121-T002 (N/A) WT MA 21029755 R Low
ZNA014-T003 cGGCATCCATgtaagtATGGATGCCg
gCCGTAGGTAcattcaTACCTACGGc
    3F+[6]+3F
(artificial) ZP00121-T003 ZP00121-T003 (N/A) WT MA 21029755 R
ZNA014-T004 cTGCATCCCTgtaagtAGGGATGCAg
gACGTAGGGAcattcaTCCCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T004 ZP00121-T004 (N/A) WT MA 21029755 R Low
ZNA014-T005 cTGCATCCGTgtaagtACGGATGCAg
gACGTAGGCAcattcaTGCCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T005 ZP00121-T005 (N/A) WT MA 21029755 R
ZNA014-T006 cTGCATCCTTgtaagtAAGGATGCAg
gACGTAGGAAcattcaTTCCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T006 ZP00121-T006 (N/A) WT MA 21029755 R
ZNA014-T007 cTGCATCCACgtaagtGTGGATGCAg
gACGTAGGTGcattcaCACCTACGTc
    3F+[6]+3F
(artificial) ZP00121-T007 ZP00121-T007 (N/A) WT MA 21029755 R
ZNA014-T008 cAGCATCCTCgtaagtGAGGATGCTg
gTCGTAGGAGcattcaCTCCTACGAc
    3F+[6]+3F
(artificial) ZP00121-T008 ZP00121-T008 (N/A) WT MA 21029755 R
ZNA014-T009 cTGCATCCATgtaagtATGGCTGCAg
gACGTAGGTAcattcaTACCGACGTc
    3F+[6]+3F
(artificial) ZP00121 ZP00121-T009 (N/A) WT MA 21029755 R
ZNA014-T010 cTGCATCCATgtaagtATGGACGCAg
gACGTAGGTAcattcaTACCTGCGTc
    3F+[6]+3F
(artificial) ZP00121 ZP00121-T010 (N/A) WT MA 21029755 R
ZN0112 gATGGGCAGCGCCcgagtGGCGGAGCTGCTg
cTACCCGTCGCGGgctcaCCGCCTCGACGAc
    4F+[5]+4F
human, CDKN2A G C ZP10695 ZP10696 TGEK WT MA(2F) NHEJ 21191366 R
ZN0113 cTCACCCGACCCGtgcacGACGCTGCCCGGg
gAGTGGGCTGGGCacgtgCTGCGACGGGCCc
    4F+[5]+4F
human, CDKN2A G C ZP10697 ZP10698 TGEK WT MA(2F) NHEJ 21191366 R
ZN0114 gGCGGCGTGCTCGcggtgcGGAGACCGGAAGg
cCGCCGCACGAGCgccacgCCTCTGGCCTTCc
    4F+[6]+4F
mouse, Thumpd3, 5'-UTR G ZP10699 ZP10700 TGEK WT MA(2F) NHEJ 21191366 R
ZNA006D nACCATCTTCcttaaaAGGGATAACn
nTGGTAGAAGgaatttTCCCTATTGn
    3F+[6]+3F
(artificial) ZP00106; ZP00129 ZP00107; ZP00130 AAARA(5aa?) D+R MA 21528840 R
ZNA006E nACCATCTTCACCATCTTCcttaaaAGGGATAACAGGGATAACn
nTGGTAGAAGTGGTAGAAGgaatttTCCCTATTGTCCCTATTGn
    6F+[6]+6F
(artificial) ZP00106; ZP00129; ZP00131; ZP00133 ZP00107; ZP00130; ZP00132; ZP00134 AAARA(5aa?) D+R MA 21528840 R
ZNA006F nACCATCTTCACCATCTTCn(x)AGGGATAACAGGGATAACn
nTGGTAGAAGTGGTAGAAGn(x)TCCCTATTGTCCCTATTGn
    6F+[x]+6F
(artificial) ZP00133 ZP00134 AAARA(5aa?) D+R MA 21528840 R
ZN0130 tCCCCTGCTTGGCtgggcgCAGTGGCTCATGc
aGGGGACGAACCGacccgcGTCACCGAGTACg
    4F+[6]+4F
human, TP53, intron G C ZP10734 ZP10735 QNKK WT, EL+KK MMA NHEJ; HR 21695267 R
ZN0131 cCACCATCCACTAcaactaCATGTGTAACAGt
gGTGGTAGGTGATgttgatGTACACATTGTCa
    4F+[6]+4F
human, TP53 G C ZP10736 ZP10737 QNKK WT, EL+KK MMA NHEJ; HR 21695267 R
ZN0150 aCTCATCCACcagctGGAGATGGCa
tGAGTAGGTGgtcgaCCTCTACCGt
    3F+[5]+3F
zebrafish, ago2 G C ZP10774 ZP10773 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0151 aATCCGTGACggcgcaGTAAGGGCCc
tTAGGCACTGccgcgtCATTCCCGGg
    3F+[6]+3F
zebrafish, arhgef1 G C ZP10775 ZP10776 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0152 aCACCTCCTCctgggtGATGGGGTGg
tGTGGAGGAGgacccaCTACCCCACc
    3F+[6]+3F
zebrafish, braf G C ZP10777 ZP10778 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0153 cCTCCTCCCCagagccGTAGTCGTAc
gGAGGAGGGGtctcggCATCAGCATg
    3F+[6]+3F
zebrafish, chd5 G C ZP10780 ZP10781 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0154 aCCCCTGGTCcggaaGTGTGGGTGt
tGGGGACCAGgccttCACACCCACa
    3F+[5]+3F
zebrafish, col18a1 G C ZP10782 ZP10783 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0155 cCTCAACACCgtcccGCAACGGAGc
gGAGTTGTGGcagggCGTTGCCTCg
    3F+[5]+3F
zebrafish, cxcl12b G C ZP10785 ZP10784 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0064B cGTCTGCACCtctcaGTGGCGGACc
gCAGACGTGGagagtCACCGCCTGg
    3F+[5]+3F
zebrafish, cxcr4a G C ZP10482 ZP10483 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0156 aCTCTCCTTCtccccGAGGGGGTCc
tGAGAGGAAGaggggCTCCCCCAGg
    3F+[5]+3F
zebrafish, dab2 G C ZP10786 ZP10787 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0157 tTTCCTCCACaaagaGGCTGGGATg
aAAGGAGGTGtttctCCGACCCTAc
    3F+[5]+3F
zebrafish, dicer1 G C ZP10789 ZP10790 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0158 tGTCCTCCTCatggcgGTCGATGGTt
aCAGGAGGAGtaccgcCAGCTACCAa
    3F+[6]+3F
zebrafish, drosha G C ZP10791 ZP10792 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0159 cCACGCCCAAgagggGCGGCAGCGg
gGTGCGGGTTctcccCGCCGTCGCc
    3F+[5]+3F
zebrafish, efnb2a G C ZP10794 ZP10793 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0160 aCCCCATTCCccctcaGCAAGAGTGg
tGGGGTAAGGgggagtCGTTCTCACc
    3F+[6]+3F
zebrafish, fli1a G C ZP10798 ZP10797 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0161 cCGCCGCCACagcgggGTGGAGGTGg
gGCGGCGGTGtcgcccCACCTCCACc
    3F+[6]+3F
zebrafish, gata2a G C ZP10802 ZP01801 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0162 cCTCCGCCACagaggaGTCGAGGTGg
gGAGGCGGTGtctcctCAGCTCCACc
    3F+[6]+3F
zebrafish, gata3 G C ZP10803 ZP10804 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0163 cTCCCGCACCagtttgGCATTGGGGt
gAGGGCGTGGtcaaacCGTAACCCCa
    3F+[6]+3F
zebrafish, kif1b G C ZP10805 ZP10806 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0164 aCGCCTCATCgtgacgGTGGAGGAAa
tGCGGAGTAGcactgcCACCTCCTTt
    3F+[6]+3F
zebrafish, kif1b G C ZP10807 ZP10789 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0165 aCTCTCCCCAgggagCAGGCAGCAt
tGAGAGGGGTccctcGTCCGTCGTa
    3F+[5]+3F
zebrafish, klf2a G C ZP10809 ZP10808 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0166 aGCCCTCCAAtctgaaGGTGCAGGAg
tCGGGAGGTTagacttCCACGTCCTc
    3F+[6]+3F
zebrafish, micall2 G C ZP10811 ZP10810 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0167 cACCCTCCTCaggctGTAGAGGAAg
gTGGGAGGAGtccgaCATCTCCTTc
    3F+[5]+3F
zebrafish, nf1a G C ZP10813 ZP10814 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0168 tTTCCGTTACctgtgtGAGGAGGCAg
aAAGGCAATGgacacaCTCCTCCGTc
    3F+[6]+3F
zebrafish, nf1b G C ZP10816 ZP10815 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0169 cCGTTACCTGtgtgagGAGGCAGAAa
gGCAATGGACacactcCTCCGTCTTt
    3F+[6]+3F
zebrafish, nf1b G C ZP10818 ZP10817 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0170 cATCCGCCTGccactGGTGTGGTCg
gTAGGCGGACggtgaCCACACCAGc
    3F+[5]+3F
zebrafish, numbl G C ZP10819 ZP10820 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0171 aCACCTCATCtggacGTGGAGGAGt
tGTGGAGTAGacctgCACCTCCTCa
    3F+[5]+3F
zebrafish, ptpru G C ZP10825 ZP10824 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0172 cCTCCTCCTCttcctCGGGTGGTGg
gGAGGAGGAGaaggaGCCCACCACc
    3F+[5]+3F
zebrafish, sbno2 G C ZP10828 ZP10829 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0173 gGACTACATCaatggaGGAGAGGTGt
cCTGATGTAGttacctCCTCTCCACa
    3F+[6]+3F
zebrafish, sgk1 G C ZP10831 ZP10830 (N/A) D+R, EL+KK MA NHEJ 21937602 R
ZN0174 cCTCCGCCCCttatcaCAGGTGGAGg
gGAGGCGGGGaatagtGTCCACCTCc
    3F+[6]+3F
zebrafish, spon1b G C ZP10834 ZP10835 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0175 tTCCCACCACtcagcAAGTGGGACg
aAGGGTGGTGagtcgTTCACCCTGc
    3F+[5]+3F
zebrafish, tie1 G C ZP10837 ZP10836 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0176 gACCCTCGGCcttcagCAGGTCGTAc
cTGGGAGCCGgaagtcGTCCAGCATg
    3F+[6]+3F
zebrafish, tarbp2 G C ZP10838 ZP10839 (N/A) D+R, EL+KK MA NHEJ 21937602 R No
ZN0177 cACACACCTGtgagtcCAGGAGGACg
gTGTGTGGACactcagGTCCTCCTGc
    3F+[6]+3F
zebrafish, yrk G C ZP10841 ZP10842 (N/A) D+R, EL+KK MA NHEJ 21937602 R Low
ZN0179 cCGCGTCCGCGCCatggcCATCTACAAGCAg
gGCGCAGGCGCGGtaccgGTAGATGTTCGTc
    4F+[5]+4F
human, TP53 G C ZP10845 ZP10846 TGEK Sharkey-AS+R MA(2F) NHEJ 21983922 R
ZNAU01 (N/A)
    
(artificial) (N/A) (N/A) (N/A) WT MA 22082504 R
ZN0183
(Z-S1)
ACTGTGAGAACAGGTGCAa
TGACACTCTTGTCCACGTt
    6F
chicken, OVAL, 3'-UTR G ZP10851 TG, TGS(G4S)3G) WT MA NHEJ 22132814 R
ZN0086B cTGCAACTCCAGTCTTTCTagaagaTGGGCGGGAGTCt
gACGTTGAGGTCAGAAAGAtcttctACCCGCCCTCAGa
    6F+[6]+4F
mouse, G ZP10852 ZP10853 TGAAARA(-Q) WT, Sharkey-ELD+KKR MA NHEJ; HR 22169954 R
ZN0184 aGACCCACTGagttggGCAAAGGTGGGGc
tCTGGGTGACtcaaccCGTTTCCACCCCg
    3F+[6]+4F
human, F8, intron G C ZPU0047 ZPU0048 (N/A) (N/A) MA NHEJ-Indel/Inversion 22183967 R
ZN0185 aACCCACTGCcgtgacGGAGGAGCGt
tTGGGTGACGgcactgCCTCCTCGCa
    3F+[6]+3F
yellow catfish, mstn G ZP10854 ZP10855 (N/A) EL+KK MA NHEJ 22194943 R
ZN0189 tAACCTCTGCCTCaagtacGTAGGGGTGGCCt
aTTGGAGACGGAGttcatgCATCCCCACCGGa
    4F+[6]+4F
hamster, Slc35c1 G ZP10860 ZP10861 LRGS WT MA NHEJ 22492235 R
ZN0190 tGTCCGAGTCcctgtaGACATGGACg
aCAGGCTCAGggacatCTGTACCTGc
    3F+[6]+3F
zebrafish, dab2ipb G C ZP10862 ZP10863 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0191 aAACCATACCgaccgtGGGGAACTGa
tTTGGTATGGctggcaCCCCTTGACt
    3F+[6]+3F
zebrafish, hey2 G C ZP10864 ZP10865 LRGS D+R 2F-MA NHEJ 22543349 R Low
ZN0192 tCGTGCCAGCtgctccGACTCGGCCt
aGCACGGTCGacgaggCTGAGCCGGa
    3F+[6]+3F
zebrafish, rock1 G C ZP10866 ZP10867 LRGS D+R 2F-MA NHEJ 22543349 R No
ZN0193 aCAACTCCAGTTCattttgGACATGGGTACGt
tGTTGAGGTCAAGtaaaacCTGTACCCATGCa
    4F+[6]+4F
zebrafish, zgc:77041, exon-intron G C ZP10868 ZP10869 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0194 cCCATTCCGGCTCtcgggGAAACGGGATCGt
gGGTAAGGCCGAGagcccCTTTGCCCTAGCa
    4F+[5]+4F
zebrafish, dclk2 G C ZP10870 ZP10871 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0195 cTTCATCTGCAGCttggctGTAGCAGACTTGt
gAAGTAGACGTCGaaccgaCATCGTCTGAACa
    4F+[6]+4F
zebrafish, mc4r G C ZP10872 ZP10873 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0196 tGCCTGCCCCGACagcatgGAACTGGGCCCGg
aCGGACGGGGCTGtcgtacCTTGACCCGGGCc
    4F+[6]+4F
zebrafish, lrp8 G C ZP10874 ZP10875 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0197 cCAGCTCCACGTCagcgtaGCTGCATGAAGGg
gGTCGAGGTGCAGtcgcatCGACGTACTTCCc
    4F+[6]+4F
zebrafish, mc3r G C ZP10876 ZP10877 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0198 gAACCTACGCACCtctctGGAGGGCCGTGTg
cTTGGATGCGTGGagagaCCTCCCGGCACAc
    4F+[5]+4F
zebrafish, apoeb G C ZP10878 ZP10879 LRGS D+R 2F-MA NHEJ 22543349 R
ZN0199 cATCCAATTCCTTgcttcaGCAGTGGAAGGTa
gTAGGTTAAGGAAcgaagtCGTCACCTTCCAt
    4F+[6]+4F
zebrafish, lepr, exon-intron G C ZP10880 ZP10881 LRGS D+R 2F-MA NHEJ 22543349 R Low
ZN0200 tTACCATGCCCCTctgtatCAGGAAAAGGTAa
aATGGTACGGGGAgacataGTCCTTTTCCATt
    4F+[6]+4F
zebrafish, zgc:56306 G C ZP10882 ZP10883 LRGS D+R 2F-MA NHEJ 22543349 R Low
ZN0204 tATCTTTATGatgtgCTGTGAATGt
aTAGAAATACtacacGACACTTACa
    3F+[5]+3F
mouse, Ush1c [mutated] G ZP10890; ZP10891 ZP10888; ZP10889 LRGS WT MA Cleavage 22661463 R
ZN0205 tGCTGTGAATgtatcACCAGACCAt
aCGACACTTAcatagTGGTCTGGTa
    3F+[5]+3F
mouse, Ush1c [mutated] G ZP10893, ZP10894 ZP10892 LRGS WT MA Cleavage; HR 22661463 R
ZN0218 gACCCTGAGCCCTacggcCGGGTCCGGGACc
cTGGGACTCGGGAtgccgGCCCAGGCCCTGg
    4F+[5]+4F
butterfly, cry2 G ZPU0075 ZPU0076 (N/A) D+R 2F-MA NHEJ 23009861 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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RSearch EENdb with this reference

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School of Life Sciences, Peking University, Beijing, China
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