EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN constructed bys ICA: 0 ZFN, 18 TALEN records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0158A ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    19+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158B ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATcgatggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAgctacccctag
    19+[17]+11
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158C ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCgatggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGctacccctag
    19+[17]+12
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158D ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGatggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCtacccctag
    19+[17]+13
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158E ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGAtggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTacccctag
    19+[17]+14
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158F ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATggggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTAcccctag
    19+[17]+15
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158G ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGggatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCcctag
    19+[17]+17
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158H ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGgatc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCctag
    19+[17]+18
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158I ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGAtc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCTag
    19+[17]+20
eGFP [mutated] G (std.) WT ICA HR 22740649 R
TN0158J ntCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGATc
naGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCTAg
    19+[17]+21
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158K ntccagtagcAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtcatcgTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    11+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158L ntccagtagCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtcatcGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    12+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158M ntccagtaGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtcatCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    13+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158N ntccagtAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtcaTCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    14+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158O ntccaGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggtCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    16+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158P ntccAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
naggTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    17+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158Q ntcCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
nagGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    18+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R
TN0158R nTCCAGTAGCAGACCTACGGCc...gCCGCAGTGGATCGATGGGGatc
nAGGTCATCGTCTGGATGCCGg...cGGCGTCACCTAGCTACCCCtag
    20+[17]+19
eGFP [mutated] G (std.) +63 WT ICA HR 22740649 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
GSearch EENdb with this gene symbol
RSearch EENdb with this reference

©2012-2013  PKU Zebrafish Functional Genomics Group
School of Life Sciences, Peking University, Beijing, China
Reference of EENdb    Contact us