EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN constructed bys V: 0 ZFN, 129 TALEN records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0009 tGCATACCTAATCATTATgctgaggatttggaaAGGGTGTTTATTCCTCATGGa
aCGTATGGATTAGTAATAcgactcctaaaccttTCCCACAAATAAGGAGTACCt
    17+[15]+20
human, HPRT1 G C (std.) +231 WT GG(V&B) 21493687 R
TN0010 tCCATTCCTATGACTGTAGATtttatcagactgaagAGCTATTGTGTGAGTATa
aGGTAAGGATACTGACATCTAaaatagtctgacttcTCGATAACACACTCATAt
    20+[15]+17
human, HPRT1 G C (std.) +231 WT GG(V&B) NHEJ, HR 21493687 R
TN0011 tGGATTATGCCTGGCACCATTAAAGAAAATa...tATAGATACAGAAGCGTCATCAAAGCATGCCa
aCCTAATACGGACCGTGGTAATTTCTTTTAt...aTATCTATGTCTTCGCAGTAGTTTCGTACGGt
    29+[28]+30
human, CFTR G C (others) +231 WT GG(V&B) 21493687 R
TN0012 tGGCACCATTAAAGAAAATATCATc...tAGATACAGAAGCGTCATCAAAGCATGCCa
aCCGTGGTAATTTCTTTTATAGTAg...aTCTATGTCTTCGCAGTAGTTTCGTACGGt
    23+[25]+28
human, CFTR G C (std.) +231 WT GG(V&B) (Weak) 21493687 R Low
TN0013 tATTTCGACGCTCATTCTctggccatggacttcttgAGCATCGGCTTCCGGGa
aTAAAGCTGCGAGTAAGAgaccggtacctgaagaacTCGTAGCCGAAGGCCCt
    17+[18]+16
zebrafish, hey2 G C (std.) +231 WT GG(V&B) 21493687 R
TN0014 tCCTGACCATTCATCAGCCGTCTt...cAAGATCCTTCTGATGGCCGAGGGCa
aGGACTGGTAAGTAGTCGGCAGAa...gTTCTAGGAAGACTACCGGCTCCCGt
    22+[24]+24
fruitfly, w G (others) +231 WT GG(V&B) 21493687 R
TN0015 tCCATGGCCAACACTTGTCACTACTTTc...aAGATACCCAGATCATATGAAGCGGCa
aGGTACCGGTTGTGAACAGTGATGAAAg...tTCTATGGGTCTAGTATACTTCGCCGt
    26+[25]+25
GFP G (std.) +231 WT GG(V&B) 21493687 R
TN0016 tACAACTACAACAGCCACAACGTCTa...aAGAACGGCATCAAGGTGAACTTCAAGa
aTGTTGATGTTGTCGGTGTTGCAGAt...tTCTTGCCGTAGTTCCACTTGAAGTTCt
    24+[21]+27
eGFP G (std.) +231 WT GG(V&B) 21493687 R
TN0017 tCGCAAAGAATTTCTTTAATt...cATCTTCGTTAAGTTTCCCGTGTa
aGCGTTTCTTAAAGAAATTAa...gTAGAAGCAATTCAAAGGGCACAt
    19+[15]+22
Plasmodium, PMV G (std.) +231 WT GG(V&B) 21493687 R
TN0018 tATTATTGGCCTTAACCATACTCTt...tATGTCAGAAAAATGTTTTACATGGa
aTAATAACCGGAATTGGTATGAGAa...aTACAGTCTTTTTACAAAATGTACCt
    23+[15]+24
Plasmodium, PMV G (std.) +231 WT GG(V&B) 21493687 R
TN0019 tCCATCTCCTCAAGGATGTTc...cAAGAACATTGTGAAGAGTCTAGa
aGGTAGAGGAGTTCCTACAAg...gTTCTTGTAACACTTCTCAGATCt
    19+[15]+22
thale cress, TT4 G (std.) +231 WT GG(V&B) 21493687 R
TN0020 tGTCGTCTTCTGCACTacctccggcgtcgacATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAtggaggccgcagctgTACGGACCACGACTGAt
    15+[15]+16
thale cress, TT4 G (std.) +231 WT GG(V&B) 21493687 R
TN0021 tCCGGATGCTCCTCTTgacaaggtctgtattgtcAGTTGTGGTTTGTCTa
aGGCCTACGAGGAGAActgttccagacataacagTCAACACCAAACAGAt
    15+[18]+15
thale cress, ADH1 G (std.) +231 WT GG(V&B) NHEJ, HR 21493687 R
TN0022 tACTGAATAATCAACACTtgggaatggtggttcAATGGGAGGATCGGTTCTATa
aTGACTTATTAGTTGTGAacccttaccaccaagTTACCCTCCTAGCCAAGATAt
    17+[15]+20
tobacco, SuRB G (std.) +231 WT GG(V&B) 21493687 R
TN0023 tACCTCATCAGGAACATGTTctacctatgattcccAGTGGCGGGGCTTTCAAAGa
aTGGAGTAGTCCTTGTACAAgatggatactaagggTCACCGCCCCGAAAGTTTCt
    19+[15]+19
tobacco, SuRB G (std.) +231 WT GG(V&B) 21493687 R
TNA002B tCCCAGAAGTAAAn(x)TTTACTTCTGGGa
aGGGTCTTCATTTn(x)AAATGAAGACCCt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA004B tAGACAAACCACAn(x)TGTGGTTTGTCTa
aTCTGTTTGGTGTn(x)ACACCAAACAGAt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA021 tGGCACTGCTAACCCTn(x)AGGGTTAGCAGTGCCa
aCCGTGACGATTGGGAn(x)TCCCAATCGTCACGGt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA022 tCGGTTTGCTATCGTTn(x)AACGATAGCAAACCGa
aGCCAAACGATAGCAAn(x)TTGCTATCGTTTGGCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) (Inactive) 21493687 R No
TNA023 tCCCAGAAGTAAACATn(x)ATGTTTACTTCTGGGa
aGGGTCTTCATTTGTAn(x)TACAAATGAAGACCCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA003B tCCGGATGCTCCTn(x)AGGAGCATCCGGa
aGGCCTACGAGGAn(x)TCCTCGTAGGCCt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA024 tACCCCTCTCCn(x)GGAGAGGGGTa
aTGGGGAGAGGn(x)CCTCTCCCCAt
    10+[x]+10
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA025 tCTTGTCCGCATGATTn(x)AATCATGCGGACAAGa
aGAACAGGCGTACTAAn(x)TTAGTACGCCTGTTCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA026 tATTCCCCACGAGCTCTn(x)AGAGCTCGTGGGGAATa
aTAAGGGGTGCTCGAGAn(x)TCTCGAGCACCCCTTAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA027 tAGACAGGAAGTGGAGCn(x)GCTCCACTTCCTGTCTa
aTCTGTCCTTCACCTCGn(x)CGAGGTGAAGGACAGAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA001B tATCAAGATTCTCn(x)GAGAATCTTGATa
aTAGTTCTAAGAGn(x)CTCTTAGAACTAt
    12+[x]+12
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA028 tAGACAAACCACAACTn(x)AGTTGTGGTTTGTCTa
aTCTGTTTGGTGTTGAn(x)TCAACACCAAACAGAt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA005B tACCCCTCTCCGCTn(x)AGCGGAGAGGGGTa
aTGGGGAGAGGCGAn(x)TCGCCTCTCCCCAt
    13+[x]+13
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA029 tCCGGATGCTCCTCTTn(x)AAGAGGAGCATCCGGa
aGGCCTACGAGGAGAAn(x)TTCTCCTCGTAGGCCt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TNA030 tACCCCTCTCCGCTTCTn(x)AGAAGCGGAGAGGGGTa
aTGGGGAGAGGCGAAGAn(x)TCTTCGCCTCTCCCCAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 21493687 R
TN0159 tACCAGTTCCAGCTTGCTTTa...gAAGTGACTGGACAACa
aTGGTCAAGGTCGAACGAAAt...cTTCACTGACCTGTTGt
    19+[24]+15
rat, Bmpr2 G (std.) +231 (N/A) GG(V&B) NHEJ 22749015 R
TN0161 tACATGCTGCTGTCCGTctccatcgtgtgtgATGAATATTTTCTGCCa
aTGTACGACGACAGGCAgaggtagcacacacTACTTATAAAAGACGGt
    16+[14]+16
zebrafish, slc24a5 G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0162 tGGACAATTCATCCGGCATccaagcagaggtcagAGGGTGAGAAGGTGCGCa
aCCTGTTAAGTAGGCCGTAggttcgtctccagtcTCCCACTCTTCCACGCGt
    18+[15]+17
zebrafish, ryr3 G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0163 tATTTCAGACGGATGTTtcccagctgcaaagtaACAGTGACAGGACTAAATCCa
aTAAAGTCTGCCTACAAagggtcgacgtttcatTGTCACTGTCCTGATTTAGGt
    16+[16]+20
zebrafish, tbx6l G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0164 tGGACCATTCACCCTGCATccaagcagaggtcagAGGGTGAGAAGGTCAGa
aCCTGGTAAGTGGGACGTAggttcgtctccagtcTCCCACTCTTCCAGTCt
    18+[15]+16
zebrafish, ryr1a G C (std.) +63 D+R GG(V&B) NHEJ 22916025 R
TN0165 tGTGAGCACCCAGCGGACCTCCTCTg...cATCTCTGACTGTTGTTCTGATa
aCACTCGTGGGTCGCCTGGAGGAGAc...gTAGAGACTGACAACAAGACTAt
    24+[18]+21
zebrafish, ponzr1 G C (std.) +63-Goldy, +231 WT GG(V&B) NHEJ, HR 23000899 R
TN0166 tTGCAACACTGAGCTCTGTAAACCTg...aACCTCAGGGTCCAGTCGGCAGCAGa
aACGTTGTGACTCGAGACATTTGGAc...tTGGAGTCCCAGGTCAGCCGTCGTCt
    24+[15]+24
zebrafish, crhr1, upstream G C (std.) +63-Goldy, +231 WT GG(V&B) NHEJ 23000899 R
TN0167 tACCCAGAAGACGTTTctgaggaactgattcAGGAGGCCACTCAAAGa
aTGGGTCTTCTGCAAAgactccttgactaagTCCTCCGGTGAGTTTCt
    15+[15]+16
zebrafish, msna G C (std.) +63-Goldy WT GG(V&B) NHEJ 23000899 R
TN0168 tCCACCAGAGAGTAACTacctatttctgggagACTATGTTGACAGAGGCa
aGGTGGTCTCTCATTGAtggataaagaccctcTGATACAACTGTCTCCGt
    16+[15]+17
zebrafish, ppp1cab G C (std.) +63-Goldy WT GG(V&B) NHEJ 23000899 R
TN0169 tCTCCTCAACATACATACTggagagttagttgacaAAGATGAATCATTTGTa
aGAGGAGTTGTATGTATGAcctctcaatcaactgtTTCTACTTAGTAAACAt
    18+[16]+16
zebrafish, cdh5 G C (std.) +63-Goldy WT GG(V&B) NHEJ 23000899 R
TN0170 tGTCAAATCTGCAGCTCCACGCTTc...cCAGAGTCAGAGGCAGAGGa
aCAGTTTAGACGTCGAGGTGCGAAg...gGTCTCAGTCTCCGTCTCCt
    23+[15]+18
zebrafish, crhr2, intron G C (std.) +63-Goldy WT GG(V&B) HR 23000899 R
TN0171 tATACATTTTACACACATATatatgaaactgacagtATTAATGGCCTGAACCTa
aTATGTAAAATGTGTGTATAtatactttgactgtcaTAATTACCGGACTTGGAt
    19+[16]+17
pig, SRY, 3'-downstream 1.4kb G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0172 tATATGAAACTGACAGTATtaatggcctgaacctAGCCAGAACTCAGATTGGGa
aTATACTTTGACTGTCATAattaccggacttggaTCGGTCTTGAGTCTAACCCt
    18+[15]+19
pig, SRY, 3'-downstream 1.5kb G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0173 tGCCCCCCCACACAGCTgagcccatgctgatggAGTACCCTGAGGCTATa
aCGGGGGGGTGTGTCGActcgggtacgactaccTCATGGGACTCCGATAt
    16+[16]+16
pig, RELA G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0174 tCCCACTCCCCTGGGGGCCTCTg...cCTCTCGGGGGACGAAGa
aGGGTGAGGGGACCCCCGGAGAc...gGAGAGCCCCCTGCTTCt
    21+[18]+16
pig, RELA G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R Low
TN0175 tCACCAACGGTCTCCTCTCGGGggacgaagacttctccTCCATTGCGGACATGGa
aGTGGTTGCCAGAGGAGAGCCCcctgcttctgaagaggAGGTAACGCCTGTACCt
    21+[16]+16
pig, RELA G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R Low
TN0176 tGGAACATGCATTCAACATcgccaagtatcagttAGGCATAGAGAAACTACTGGa
aCCTTGTACGTAAGTTGTAgcggttcatagtcaaTCCGTATCTCTTTGATGACCt
    18+[15]+20
pig, DMD G (std.) +63-Goldy, +231 WT GG(V&B) NHEJ, NHEJ-DisDel, NHEJ-Inv 23027955 R
TN0177 tCTATACCTAGGTCAAAAATg...cATGGCTGGATTGCAACAAACTa
aGATATGGATCCAGTTTTTAc...gTACCGACCTAACGTTGTTTGAt
    19+[15]+21
pig, DMD, intron-exon G (std.) +63-Goldy WT GG(V&B) NHEJ, NHEJ-DisDel, NHEJ-Inv 23027955 R
TN0178 tCGCCAAGTATCAGTTAGGCATa...aAGGTTAGTGCATTTTGGGa
aGCGGTTCATAGTCAATCCGTAt...tTCCAATCACGTAAAACCCt
    21+[21]+18
pig, DMD, exon-intron G (std.) +63-Goldy, +231 WT GG(V&B) 23027955 R No
TN0179 tCCTTCCAAGGCTTCTACATc...cACTTTTGATAAGACATTCTTGGTa
aGGAAGGTTCCGAAGATGTAg...gTGAAAACTATTCTGTAAGAACCAt
    19+[16]+23
pig, AMELY, Intron G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0180 tCTACATCAATCACTTGGCTttcacttttgataagACATTCTTGGTATGGATa
aGATGTAGTTAGTGAACCGAaagtgaaaactattcTGTAAGAACCATACCTAt
    19+[15]+17
pig, AMELY, Intron G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R No
TN0181 tCTGCGCTCCTTCAAAGTgtttaagatcaagcctgAGAAGAGGTGGCAGGACa
aGACGCGAGGAAGTTTCAcaaattctagttcggacTCTTCTCCACCGTCCTGt
    17+[17]+17
cow, GGTA1 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0182 tCTGCAAATACATACTTCATg...cATCTTTTACATCATGGTGGa
aGACGTTTATGTATGAAGTAc...gTAGAAAATGTAGTACCACCt
    19+[16]+19
pig, GGTA1 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0183 tGCGTTCCTTTAAAGTGTttgagatcaagtccgAGAAGAGGTGGCAAGACa
aCGCAAGGAAATTTCACAaactctagttcaggcTCTTCTCCACCGTTCTGt
    17+[15]+17
pig, GGTA1 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0184 tCCTTTCCTCCAGGGATCCCTc...cACTGGAGAAGCAACAGAGGAGCa
aGGAAAGGAGGTCCCTAGGGAg...gTGACCTCTTCGTTGTCTCCTCGt
    20+[15]+22
cow, ACAN G (std.) +63-Goldy, +231 WT GG(V&B) NHEJ 23027955 R
TN0185 tGCAATCCCAGGCTTCACCGTTg...gACGGAATGGGAACTTGCCTACa
aCGTTAGGGTCCGAAGTGGCAAc...cTGCCTTACCCTTGAACGGATGt
    21+[15]+21
cow, ACAN, intron-exon G (std.) +63-Goldy, +231 WT GG(V&B) 23027955 R Low
TN0186 tCCAGTGCAACAGCTCCACCTg...gGGCCTGTGATGGTGATCCTGa
aGGTCACGTTGTCGAGGTGGAc...cCCGGACACTACCACTAGGACt
    20+[16]+20
pig, LDLR G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0187 tCTCCTACAAGTGGATTTgtgatgggaacaccgAGTGCAAGGACGGGTCCGa
aGAGGATGTTCACCTAAAcactacccttgtggcTCACGTTCCTGCCCAGGCt
    17+[15]+18
pig, LDLR G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0188 tCCACTCCAGCTGGCGCTgcgacggagacactgACTGCAAGGACAAGTCTGACGa
aGGTGAGGTCGACCGCGAcgctgcctctgtgacTGACGTTCCTGTTCAGACTGCt
    17+[15]+21
pig, LDLR G (std.) +63-Goldy, +231 WT GG(V&B) 23027955 R No
TN0189 tGCAACAGCTCCACCTGCATCCCTg...gATGGTGATCCTGACTGCGAGGa
aCGTTGTCGAGGTGGACGTAGGGAc...cTACCACTAGGACTGACGCTCCt
    23+[16]+21
pig, LDLR G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0190 tACCTTCCTCCTCTCCGCTacccagccacttgcacATTCAAAAGCAGCTTAGa
aTGGAAGGAGGAGAGGCGAtgggtcggtgaacgtgTAAGTTTTCGTCGAATCt
    18+[16]+17
pig, RAG2 G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0191 tGGTAATAACATGGCCTTAATt...cATTGATGAATTTTGATGGGCa
aCCATTATTGTACCGGAATTAa...gTAACTACTTAAAACTACCCGt
    20+[15]+20
pig, RAG2 G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0192 tCCCAAAGGTTCAGTGTTTtgtgttcaatgttgAGTACATGAATTGCACTTGGa
aGGGTTTCCAAGTCACAAAacacaagttacaacTCATGTACTTAACGTGAACCt
    18+[14]+20
pig, IL2RG G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0193 tCTACCAAACATTTGTTGTccagctccaggacccACGGGAACCCAGGAGGCAGGa
aGATGGTTTGTAAACAACAggtcgaggtcctgggTGCCCTTGGGTCCTCCGTCCt
    18+[15]+20
pig, IL2RG G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R No
TN0194 tCCCCTGCCCGGCTTTCTTCTctcacttcagctctgAGCCAGGTGAGGGGGa
aGGGGACGGGCCGAAAGAAGAgagtgaagtcgagacTCGGTCCACTCCCCCt
    20+[15]+15
pig, GHRHR, intron-exon G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0195 tCCCATCTCGCCGTCCAGGCTg...gATGGGCTGTTGTGCTGGCCGa
aGGGTAGAGCGGCAGGTCCGAc...cTACCCGACAACACGACCGGCt
    20+[15]+20
pig, GHRHR, exon-intron G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0196 tCCCTTTTTAGAAGTCAAGGTa...aAAGATCCAGGAGAGATTTTGGa
aGGGAAAAATCTTCAGTTCCAt...tTTCTAGGTCCTCTCTAAAACCt
    20+[15]+21
pig, MSTN G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0197 tGGATTATTGCACCCAAAAGATa...gCTCTGGAGAGTGTGAATTTGTa
aCCTAATAACGTGGGTTTTCTAt...cGAGACCTCTCACACTTAAACAt
    21+[16]+21
pig, MSTN G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0198 tCTAACTGTGGATTTTGAAGCTtttggatgggactggATTATTGCACCCAAAAGa
aGATTGACACCTAAAACTTCGAaaacctaccctgaccTAATAACGTGGGTTTTCt
    21+[15]+17
pig, MSTN G (std.) +63-Goldy, +231 WT GG(V&B) NHEJ 23027955 R Low
TN0199A tGTGATGAACACTCCACAGAATCTc...cCTCTAACTGTGGATTTTGAa
aCACTACTTGTGAGGTGTCTTAGAg...gGAGATTGACACCTAAAACTt
    23+[16]+19
cow, MSTN G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R
TN0199B tGTGATGAACACTCCACAGAATCTc...aACTGTGGATTTTGAAGCTTTTGGa
aCACTACTTGTGAGGTGTCTTAGAg...tTGACACCTAAAACTTCGAAAACCt
    23+[21]+23
cow, MSTN G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0200 tGCAAGAAGCGACCAAAACCTggaggaggatggaacACTGGGGGGAGCCGATa
aCGTTCTTCGCTGGTTTTGGAcctcctcctaccttgTGACCCCCCTCGGCTAt
    20+[15]+16
cow, PRNP G (std.) +63-Goldy WT GG(V&B) NHEJ 23027955 R Low
TN0201 tCAATGGAACAAACCCAGTAAg...gAAGCATGTGGCAGGAGCTGCTGCa
aGTTACCTTGTTTGGGTCATTc...cTTCGTACACCGTCCTCGACGACGt
    20+[16]+23
cow, PRNP G (std.) +63-Goldy, +231 WT GG(V&B) 23027955 R No
TN0202 tGTTGAGTGACAATTTCTCCCTCCTGCAGGTt...gATGAGGGTTCTGTGGGTTGCTTTGGTGGTa
aCAACTCACTGTTAAAGAGGGAGGACGTCCAa...cTACTCCCAAGACACCCAACGAAACCACCAt
    30+[21]+29
pig, APOE, intron-exon G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0203 tGTTCCCCAGGCCTGGTTCATACTCTt...tACCAGGAGGTGGGAGCCTTGGGTACCa
aCAAGGGGTCCGGACCAAGTATGAGAa...aTGGTCCTCCACCCTCGGAACCCATGGt
    25+[18]+26
pig, HR G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0204 tGGATGAAACCGAAATTagaagtttctttgctagATATGGTTCAGTAAAAGa
aCCTACTTTGGCTTTAAtcttcaaagaaacgatcTATACCAAGTCATTTTCt
    16+[17]+17
pig, DAZL G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TN0205 tCCAAGCACTGTTTAAGTctggatgttggtcaaaACCCAATTTGATGATGTa
aGGTTCGTGACAAATTCAgacctacaaccagtttTGGGTTAAACTACTACAt
    17+[16]+17
cow, OFD1Y G (std.) +63-Goldy WT GG(V&B) 23027955 R No
TNA034 tGTCGTCTTCTGCACTn(x)AGTGCAGAAGACGACa
aCAGCAGAAGACGTGAn(x)TCACGTCTTCTGCTGt
    15+[x]+15
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA034B tGTCGTCTTCTGCACTn(x)AGTGCAGAAGACGACa
aCAGCAGAAGACGTGAn(x)TCACGTCTTCTGCTGt
    15+[x]+15
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA034C tGTCGTCTTCTGCACTn(x)AGTGCAGAAGACGACa
aCAGCAGAAGACGTGAn(x)TCACGTCTTCTGCTGt
    15+[x]+15
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA034D tGTCGTCTTCTGCACTn(x)AGTGCAGAAGACGACa
aCAGCAGAAGACGTGAn(x)TCACGTCTTCTGCTGt
    15+[x]+15
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA035 tAGTCAGCACCAGGCATn(x)ATGCCTGGTGCTGACTa
aTCAGTCGTGGTCCGTAn(x)TACGGACCACGACTGAt
    16+[x]+16
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA035B tAGTCAGCACCAGGCATn(x)ATGCCTGGTGCTGACTa
aTCAGTCGTGGTCCGTAn(x)TACGGACCACGACTGAt
    16+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA035C tAGTCAGCACCAGGCATn(x)ATGCCTGGTGCTGACTa
aTCAGTCGTGGTCCGTAn(x)TACGGACCACGACTGAt
    16+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R Low
TNA035D tAGTCAGCACCAGGCATn(x)ATGCCTGGTGCTGACTa
aTCAGTCGTGGTCCGTAn(x)TACGGACCACGACTGAt
    16+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA036 tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA036-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R No
TNA036B tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R Low
TNA036B-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R
TNA036C tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA036C-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R
TNA036D tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA036D-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R
TNA036E tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA036E-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R
TNA036F tGTCGTCTTCTGCACTn(x)ATGCCTGGTGCTGACTa
aCAGCAGAAGACGTGAn(x)TACGGACCACGACTGAt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA036F-T002 tATCATCTTCTACACTn(x)ATGTTTGGTGTTGATTa
aTAGTAGAAGATGTGAn(x)TACAAACCACAACTAAt
    18+[x]+20
(artificial) +231 WT GG(V&B) 23028976 R Low
TNA037 tCCATTCCTATGACTGTn(x)AGCTATTGTGTGAGTATa
aGGTAAGGATACTGACAn(x)TCGATAACACACTCATAt
    16+[x]+17
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA037B tCCATTCCTATGACTGTn(x)AGCTATTGTGTGAGTATa
aGGTAAGGATACTGACAn(x)TCGATAACACACTCATAt
    16+[x]+17
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA038 tCCCTCGCTTTCTGATn(x)ATCGGGCGCTGTCGGGa
aGGGAGCGAAAGACTAn(x)TAGCCCGCGACAGCCCt
    15+[x]+16
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA038B tCCCTCGCTTTCTGATn(x)ATCGGGCGCTGTCGGGa
aGGGAGCGAAAGACTAn(x)TAGCCCGCGACAGCCCt
    15+[x]+16
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA039 tCCTTTTAAGAAGTTGGCATn(x)ATAATACAATTTAAAACCTGGGTCTCTa
aGGAAAATTCTTCAACCGTAn(x)TATTATGTTAAATTTTGGACCCAGAGAt
    19+[x]+27
(artificial) (std.) +231 WT GG(V&B) 23028976 R Low
TNA039B tCCTTTTAAGAAGTTGGCATn(x)ATAATACAATTTAAAACCTGGGTCTCTa
aGGAAAATTCTTCAACCGTAn(x)TATTATGTTAAATTTTGGACCCAGAGAt
    19+[x]+27
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA040 tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA040-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R No
TNA040B tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA040B-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R No
TNA040C tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA040C-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R
TNA040D tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA040D-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R No
TNA040E tGGAGAATTCCCACTTTn(x)AGTCGCCCAGTTTTTATGAACa
aCCTCTTAAGGGTGAAAn(x)TCAGCGGGTCAAAAATACTTGt
    16+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA040E-T002 tAAAAAATTCCCACTTTn(x)AGTTGTTTAGTTTTTATGAATa
aTTTTTTAAGGGTGAAAn(x)TCAACAAATCAAAAATACTTAt
    19+[x]+26
(artificial) +231 WT GG(V&B) 23028976 R No
TNA041 tGCTGACGACTTTGTCCCTn(x)ACTCGGTGGGCTTGTTGGCGGa
aCGACTGCTGAAACAGGGAn(x)TGAGCCACCCGAACAACCGCCt
    18+[x]+21
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA041B tGCTGACGACTTTGTCCCTn(x)ACTCGGTGGGCTTGTTGGCGGa
aCGACTGCTGAAACAGGGAn(x)TGAGCCACCCGAACAACCGCCt
    18+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA042 tCTTTACAAGGAGCACGTn(x)ATTAAAGGAGTTTAGCGTGACa
aGAAATGTTCCTCGTGCAn(x)TAATTTCCTCAAATCGCACTGt
    17+[x]+21
(artificial) (std.) +231 WT GG(V&B) 23028976 R Low
TNA042B tCTTTACAAGGAGCACGTn(x)ATTAAAGGAGTTTAGCGTGACa
aGAAATGTTCCTCGTGCAn(x)TAATTTCCTCAAATCGCACTGt
    17+[x]+21
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA043 tCTACCTTCATTGATGTCCCTn(x)ATGAAAGGCAAAGCTCCTCTa
aGATGGAAGTAACTACAGGGAn(x)TACTTTCCGTTTCGAGGAGAt
    20+[x]+20
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA043B tCTACCTTCATTGATGTCCCTn(x)ATGAAAGGCAAAGCTCCTCTa
aGATGGAAGTAACTACAGGGAn(x)TACTTTCCGTTTCGAGGAGAt
    20+[x]+20
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA044 tAGCTCAGTCATCAAAGGCAAAGCTn(x)ACTGGCTCTGGAGGTTACa
aTCGAGTCAGTAGTTTCCGTTTCGAn(x)TGACCGAGACCTCCAATGt
    24+[x]+18
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA044B tAGCTCAGTCATCAAAGGCAAAGCTn(x)ACTGGCTCTGGAGGTTACa
aTCGAGTCAGTAGTTTCCGTTTCGAn(x)TGACCGAGACCTCCAATGt
    24+[x]+18
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA045 tATCCAAAAGAGCGGTCTGATn(x)ATCCTCAGGATTGTTGCa
aTAGGTTTTCTCGCCAGACTAn(x)TAGGAGTCCTAACAACGt
    20+[x]+17
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA045B tATCCAAAAGAGCGGTCTGATn(x)ATCCTCAGGATTGTTGCa
aTAGGTTTTCTCGCCAGACTAn(x)TAGGAGTCCTAACAACGt
    20+[x]+17
(artificial) (NK) +231 WT GG(V&B) 23028976 R Low
TNA046 tGGCGAAAGAGTCCACCACCATn(x)AAGTTCAAGCCATGTCGTa
aCCGCTTTCTCAGGTGGTGGTAn(x)TTCAAGTTCGGTACAGCAt
    21+[x]+18
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA046B tGGCGAAAGAGTCCACCACCATn(x)AAGTTCAAGCCATGTCGTa
aCCGCTTTCTCAGGTGGTGGTAn(x)TTCAAGTTCGGTACAGCAt
    21+[x]+18
(artificial) (NK) +231 WT GG(V&B) 23028976 R No
TNA047 tGCTTGCTTACCTGACCTATn(x)ACATATTTTTGTTTTCAGa
aCGAACGAATGGACTGGATAn(x)TGTATAAAAACAAAAGTCt
    19+[x]+18
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA047B tGCTTGCTTACCTGACCTATn(x)ACATATTTTTGTTTTCAGa
aCGAACGAATGGACTGGATAn(x)TGTATAAAAACAAAAGTCt
    19+[x]+18
(artificial) (NK) +231 WT GG(V&B) 23028976 R
TNA048 tCGGCCTGCCGGCCCTGGCCGGTn(x)AGGTGGCGGCCGGGCCCTGAGGCGCGGGa
aGCCGGACGGCCGGGACCGGCCAn(x)TCCACCGCCGGCCCGGGACTCCGCGCCCt
    22+[x]+28
(artificial) (std.) +231 WT GG(V&B) 23028976 R
TNA048B tCGGCCTGCCGGCCCTGGCCGGTn(x)AGGTGGCGGCCGGGCCCTGAGGCGCGGGa
aGCCGGACGGCCGGGACCGGCCAn(x)TCCACCGCCGGCCCGGGACTCCGCGCCCt
    22+[x]+28
(artificial) (NK) +231 WT GG(V&B) 23028976 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
GSearch EENdb with this gene symbol
RSearch EENdb with this reference

©2012-2013  PKU Zebrafish Functional Genomics Group
School of Life Sciences, Peking University, Beijing, China
Reference of EENdb    Contact us