EENdb - TALEN/ZFN
Search TALENs/ZFNs targeting the sequence:
- >=3 nt (recommend >=6 nt), 5'->3', any strand
- use [...] for alternate nucleotides; N = [ACGT]
Search TALENs/ZFNs targeting the gene(s):
- <=10 symbols or IDs (currently Ensembl ID only)
- symbols are variable in publications and databases
Search EENs by first reported reference:
- <=10 PubMed IDs or surnames of 1st authors.

EEN Search Result
EEN constructed bys FLASH: 0 ZFN, 161 TALEN records match.
(Click on ID to see details)


TALEN IDWhole SiteSpecies & LocusRVDsFrameworkFokIConstruc.Modif.Ref.Effic.
TN0041A tCGCCACCATggtgagcaagggcgagGAGCTGTTCa
aGCGGTGGTAccactcgttcccgctcCTCGACAAGt
    9+[16]+9
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041B tGGTGCCCATcctggtcgagctggacGGCGACGTAa
aCCACGGGTAggaccagctcgacctgCCGCTGCATt
    9+[16]+9
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041C tCTGCACCACcggcaagctgcccgtgCCCTGGCCCa
aGACGTGGTGgccgttcgacgggcacGGGACCGGGt
    9+[16]+9
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041D tGGAGTACAActacaacagccacaacGTCTATATCa
aCCTCATGTTgatgttgtcggtgttgCAGATATAGt
    9+[16]+9
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041E tTCAGCGTGTCcggcgagggcgagggcGATGCCACCTa
aAGTCGCACAGgccgctcccgctcccgCTACGGTGGAt
    10+[16]+10
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041F tGCCACCTACGgcaagctgaccctgaaGTTCATCTGCa
aCGGTGGATGCcgttcgactgggacttCAAGTAGACGt
    10+[16]+10
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041G tGGCCCACCCTcgtgaccaccctgaccTACGGCGTGCa
aCCGGGTGGGAgcactggtgggactggATGCCGCACGt
    10+[16]+10
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041H tTCAAGATCCGccacaacatcgaggacGGCAGCGTGCa
aAGTTCTAGGCggtgttgtagctcctgCCGTCGCACGt
    10+[16]+10
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041I tAGAGGATCCACcggtcgccaccatggtGAGCAAGGGCGa
aTCTCCTAGGTGgccagcggtggtaccaCTCGTTCCCGCt
    11+[16]+11
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041J tCCGGCGAGGGCgagggcgatgccacctACGGCAAGCTGa
aGGCCGCTCCCGctcccgctacggtggaTGCCGTTCGACt
    11+[16]+11
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041K tGACCTACGGCGtgcagtgcttcagccgCTACCCCGACCa
aCTGGATGCCGCacgtcacgaagtcggcGATGGGGCTGGt
    11+[16]+11
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041L tCCGCCACAACAtcgaggacggcagcgtGCAGCTCGCCGa
aGGCGGTGTTGTagctcctgccgtcgcaCGTCGAGCGGCt
    11+[16]+11
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041M tCCTGGTCGAGCTggacggcgacgtaaacGGCCACAAGTTCa
aGGACCAGCTCGAcctgccgctgcatttgCCGGTGTTCAAGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041N tCAGCCGCTACCCcgaccacatgaagcagCACGACTTCTTCa
aGTCGGCGATGGGgctggtgtacttcgtcGTGCTGAAGAAGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041O tCTTCAAGTCCGCcatgcccgaaggctacGTCCAGGAGCGCa
aGAAGTTCAGGCGgtacgggcttccgatgCAGGTCCTCGCGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041P tCAAGGAGGACGGcaacatcctggggcacAAGCTGGAGTACa
aGTTCCTCCTGCCgttgtaggaccccgtgTTCGACCTCATGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041Q tCAAGGTGAACTTcaagatccgccacaacATCGAGGACGGCa
aGTTCCACTTGAAgttctaggcggtgttgTAGCTCCTGCCGt
    12+[16]+12
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041R tCCACCGGTCGCCAccatggtgagcaagggCGAGGAGCTGTTCa
aGGTGGCCAGCGGTggtaccactcgttcccGCTCCTCGACAAGt
    13+[16]+13
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041S tTCAGCGTGTCCGGcgagggcgagggcgatGCCACCTACGGCAa
aAGTCGCACAGGCCgctcccgctcccgctaCGGTGGATGCCGTt
    13+[16]+13
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041T tTCAGCCGCTACCCcgaccacatgaagcagCACGACTTCTTCAa
aAGTCGGCGATGGGgctggtgtacttcgtcGTGCTGAAGAAGTt
    13+[16]+13
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041U tACAACAGCCACAAcgtctatatcatggccGACAAGCAGAAGAa
aTGTTGTCGGTGTTgcagatatagtaccggCTGTTCGTCTTCTt
    13+[16]+13
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041V tGGTGCCCATCCTGGtcgagctggacggcgaCGTAAACGGCCACAa
aCCACGGGTAGGACCagctcgacctgccgctGCATTTGCCGGTGTt
    14+[16]+14
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041W tGCCCGAAGGCTACGtccaggagcgcaccatCTTCTTCAAGGACGa
aCGGGCTTCCGATGCaggtcctcgcgtggtaGAAGAAGTTCCTGCt
    14+[16]+14
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041X tGAACCGCATCGAGCtgaagggcatcgacttCAAGGAGGACGGCAa
aCTTGGCGTAGCTCGacttcccgtagctgaaGTTCCTCCTGCCGTt
    14+[16]+14
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041Y tCAAGATCCGCCACAacatcgaggacggcagCGTGCAGCTCGCCGa
aGTTCTAGGCGGTGTtgtagctcctgccgtcGCACGTCGAGCGGCt
    14+[16]+14
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041Z tGTTCACCGGGGTGGTgcccatcctggtcgagCTGGACGGCGACGTAa
aCAAGTGGCCCCACCAcgggtaggaccagctcGACCTGCCGCTGCATt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AA tGCCCATCCTGGTCGAgctggacggcgacgtaAACGGCCACAAGTTCa
aCGGGTAGGACCAGCTcgacctgccgctgcatTTGCCGGTGTTCAAGt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AB tGTCCGGCGAGGGCGAgggcgatgccacctacGGCAAGCTGACCCTGa
aCAGGCCGCTCCCGCTcccgctacggtggatgCCGTTCGACTGGGACt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AC tCATCTGCACCACCGGcaagctgcccgtgcccTGGCCCACCCTCGTGa
aGTAGACGTGGTGGCCgttcgacgggcacgggACCGGGTGGGAGCACt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AD tCTATATCATGGCCGAcaagcagaagaacggcATCAAGGTGAACTTCa
aGATATAGTACCGGCTgttcgtcttcttgccgTAGTTCCACTTGAAGt
    15+[16]+15
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AE tACGGCGTGCAGTGCTTcagccgctaccccgacCACATGAAGCAGCACGa
aTGCCGCACGTCACGAAgtcggcgatggggctgGTGTACTTCGTCGTGCt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AF tACCCCGACCACATGAAgcagcacgacttcttcAAGTCCGCCATGCCCGa
aTGGGGCTGGTGTACTTcgtcgtgctgaagaagTTCAGGCGGTACGGGCt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AG tCCGCCATGCCCGAAGGctacgtccaggagcgcACCATCTTCTTCAAGGa
aGGCGGTACGGGCTTCCgatgcaggtcctcgcgTGGTAGAAGAAGTTCCt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AH tTCAAGGAGGACGGCAAcatcctggggcacaagCTGGAGTACAACTACAa
aAGTTCCTCCTGCCGTTgtaggaccccgtgttcGACCTCATGTTGATGTt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AI tACAACTACAACAGCCAcaacgtctatatcatgGCCGACAAGCAGAAGAa
aTGTTGATGTTGTCGGTgttgcagatatagtacCGGCTGTTCGTCTTCTt
    16+[16]+16
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AJ tCACCGGGGTGGTGCCCAtcctggtcgagctggaCGGCGACGTAAACGGCCa
aGTGGCCCCACCACGGGTaggaccagctcgacctGCCGCTGCATTTGCCGGt
    17+[16]+17
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AK tAAACGGCCACAAGTTCAgcgtgtccggcgagggCGAGGGCGATGCCACCTa
aTTTGCCGGTGTTCAAGTcgcacaggccgctcccGCTCCCGCTACGGTGGAt
    17+[16]+17
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AL tGCAGTGCTTCAGCCGCTaccccgaccacatgaaGCAGCACGACTTCTTCAa
aCGTCACGAAGTCGGCGAtggggctggtgtacttCGTCGTGCTGAAGAAGTt
    17+[16]+17
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AM tCAGCGTGTCCGGCGAGGGcgagggcgatgccaccTACGGCAAGCTGACCCTGa
aGTCGCACAGGCCGCTCCCgctcccgctacggtggATGCCGTTCGACTGGGACt
    18+[16]+18
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AN tGAAGTTCATCTGCACCACcggcaagctgcccgtgCCCTGGCCCACCCTCGTGa
aCTTCAAGTAGACGTGGTGgccgttcgacgggcacGGGACCGGGTGGGAGCACt
    18+[16]+18
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AO tCGTGACCACCCTGACCTAcggcgtgcagtgcttcAGCCGCTACCCCGACCACa
aGCACTGGTGGGACTGGATgccgcacgtcacgaagTCGGCGATGGGGCTGGTGt
    18+[16]+18
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AP tCATGGCCGACAAGCAGAAgaacggcatcaaggtgAACTTCAAGATCCGCCACa
aGTACCGGCTGTTCGTCTTcttgccgtagttccacTTGAAGTTCTAGGCGGTGt
    18+[16]+18
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AQ tTCACCGGGGTGGTGCCCATc...cGGCGACGTAAACGGCCACAa
aAGTGGCCCCACCACGGGTAg...gCCGCTGCATTTGCCGGTGTt
    19+[16]+19
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AR tGCACCACCGGCAAGCTGCCc...cCTCGTGACCACCCTGACCTa
aCGTGGTGGCCGTTCGACGGg...gGAGCACTGGTGGGACTGGAt
    19+[16]+19
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AS tTCAAGTCCGCCATGCCCGAa...gCGCACCATCTTCTTCAAGGa
aAGTTCAGGCGGTACGGGCTt...cGCGTGGTAGAAGAAGTTCCt
    19+[16]+19
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AT tATATCATGGCCGACAAGCAg...gGTGAACTTCAAGATCCGCCa
aTATAGTACCGGCTGTTCGTc...cCACTTGAAGTTCTAGGCGGt
    19+[16]+19
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AU tTCATCTGCACCACCGGCAAGc...cCCACCCTCGTGACCACCCTGa
aAGTAGACGTGGTGGCCGTTCg...gGGTGGGAGCACTGGTGGGACt
    20+[16]+20
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0041AV tGAAGTTCGAGGGCGACACCCt...aGCTGAAGGGCATCGACTTCAa
aCTTCAAGCTCCCGCTGTGGGa...tCGACTTCCCGTAGCTGAAGTt
    20+[16]+20
eGFP G (std.) +63 WT FLASH NHEJ 22484455 R
TN0042 tACCTATTATTACTTTATggggcagcagcctggaaAAGTACTTGGGGACCAa
aTGGATAATAATGAAATAccccgtcgtcggaccttTTCATGAACCCCTGGTt
    17+[17]+16
human, ABL1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0043 tGTGTCTTGGGATGAGTGggtcagtgttctggtgCTCACAGGATGGCTGGCa
aCACAGAACCCTACTCACccagtcacaagaccacGAGTGTCCTACCGACCGt
    17+[16]+17
human, AKT2 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0044 tCCTGTGGCTCCTGCCGCtgctgctttccacggcAGCTGTGGGCTCCGGGa
aGGACACCGAGGACGGCGacgacgaaaggtgccgTCGACACCCGAGGCCCt
    17+[16]+16
human, ALK G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0045 tATGTACGCCTCCCTGGGctcgggtccggtcgccCCTTTGCCCGCTTCTGTa
aTACATGCGGAGGGACCCgagcccaggccagcggGGAAACGGGCGAAGACAt
    17+[16]+17
human, APC, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0046 tGAATTGGGATGCTGTTTttaggtattctattcaaaTTTATTTTACTGTCTTTa
aCTTAACCCTACGACAAAaatccataagataagtttAAATAAAATGACAGAAAt
    17+[18]+17
human, ATM G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0047 tCCCTCACCATGAGTAGCgctatgttggtgacttGCCTCCCGGACCCCAGCa
aGGGAGTGGTACTCATCGcgatacaaccactgaaCGGAGGGCCTGGGGTCGt
    17+[16]+17
human, AXIN2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0048 tGTGCGATCTCCAAGCACtgaggggcagaaactcCCGGATCGGGCGCTGCCa
aCACGCTAGAGGTTCGTGactccccgtctttgagGGCCTAGCCCGCGACGGt
    17+[16]+17
human, BAX G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0049 tTTTCAAGTGAAGACAAAatggcctcgccggctgACAGCTGTATCCAGTTCa
aAAAGTTCACTTCTGTTTtaccggagcggccgacTGTCGACATAGGTCAAGt
    17+[16]+17
human, BCL6, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0050 tACAATTGAACAATGCCTcagctatacatttacatCAGATTATTGGGAGCCTa
aTGTTAACTTGTTACGGAgtcgatatgtaaatgtaGTCTAATAACCCTCGGAt
    17+[17]+17
human, BMPR1A, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0051 tCCGAAGCTGACAGATGGgtattctttgacggggGGTAGGGGCGGAACCTGa
aGGCTTCGACTGTCTACCcataagaaactgccccCCATCCCCGCCTTGGACt
    17+[16]+17
human, BRCA1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0052 tTAGACTTAGGTAAGTAAtgcaatatggtagactGGGGAGAACTACAAACTa
aATCTGAATCCATTCATTacgttataccatctgaCCCCTCTTGATGTTTGAt
    17+[16]+17
human, BRCA2 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0053 tCTGCAATAAAAAATGGCctccaacaaaactacaTTGGTAAGTTAATGAAAa
aGACGTTATTTTTTACCGgaggttgttttgatgtAACCATTCAATTACTTTt
    17+[16]+17
human, CBX3, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0054 tGGAGCTTTCAGCGGTGGgggagcgggtgttcgcgGCCGAAGCCCTCCTGAa
aCCTCGAAAGTCGCCACCccctcgcccacaagcgcCGGCTTCGGGAGGACTt
    17+[17]+16
human, CBX8, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0055 tGGAACACCAGCTCCTGTgctgcgaagtggaaaccatCCGCCGCGCGTACCCCGa
aCCTTGTGGTCGAGGACAcgacgcttcacctttggtaGGCGGCGCGCATGGGGCt
    17+[19]+17
human, CCND1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0056 tGCTTAGCGTCCTGCGACagtacaacatccagaaGAAGGAGATTGTGGTGAa
aCGAATCGCAGGACGCTGtcatgttgtaggtcttCTTCCTCTAACACCACTt
    17+[16]+17
human, CDC73 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0057 tGCTGCAGGTACCCCGGAtcccctgacttgcgagGGACGCATTCGGGCCGCa
aCGACGTCCATGGGGCCTaggggactgaacgctcCCTGCGTAAGCCCGGCGt
    17+[16]+17
human, CDH1 G C (std.) +63 WT FLASH 22484455 R No
TN0058 tCCCTTGATCTGAGAAtggctacctctcgataTGAGCCAGTGGCTGAAa
aGGGAACTAGACTCTTaccgatggagagctatACTCGGTCACCGACTTt
    15+[16]+16
human, CDK4, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0059 tGGCGTCGGGCCTGGGCtccccgtccccctgctcGGCGGGCAGTGAGGAGGa
aCCGCAGCCCGGACCCGaggggcagggggacgagCCGCCCGTCACTCCTCCt
    16+[17]+17
human, CHD4, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0060 tGTGTTGGAAGAAGATGGcagatccaggaatgatGAGTCTTTTTGGCGAGGa
aCACAACCTTCTTCTACCgtctaggtccttactaCTCAGAAAAACCGCTCCt
    17+[16]+17
human, CHD7, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0061 tCCAGCGTGGACAATGGctactcaaggtttgtgTCATTAAATCTTTAGTTa
aGGTCGCACCTGTTACCgatgagttccaaacacAGTAATTTAGAAATCAAt
    16+[16]+17
human, CTNNB1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0062 tAATATCACAATGAGTTCaggcttatggagccaagaAAAAGTCACTTCACCCTa
aTTATAGTGTTACTCAAGtccgaatacctcggttctTTTTCAGTGAAGTGGGAt
    17+[18]+17
human, CYLD, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0063 tCACACGGAGGACGCGatggctcccaagaaacGCCCAGAAACCCAGAAGa
aGTGTGCCTCCTGCGCtaccgagggttctttgCGGGTCTTTGGGTCTTCt
    15+[16]+17
human, DDB2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0064 tCCGGCCGGCGCCATGAagtgagaagggggctgGGGGTCGCGCTCGCTa
aGGCCGGCCGCGGTACTtcactcttcccccgacCCCCAGCGCGAGCGAt
    16+[16]+15
human, ERCC2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0065 tCCGGGATCGCCATGGGAactcaatagaaaatcctcaTCTTCTCACTTTGTTTCa
aGGCCCTAGCGGTACCCTtgagttatcttttaggagtAGAAGAGTGAAACAAAGt
    17+[19]+17
human, ERCC5 G C (std.) +63 WT FLASH 22484455 R No
TN0066 tGGCGTCCACGGGTGAGTatggtggaactgcggtcGCGCCGGCGGTAGCCGGa
aCCGCAGGTGCCCACTCAtaccaccttgacgccagCGCGGCCGCCATCGGCCt
    17+[17]+17
human, EWSR1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0067 tGACCCAGGCAGGACACAtgcaggccaaaaaacgcTATTTCATCCTGCTCTCa
aCTGGGTCCGTCCTGTGTacgtccggttttttgcgATAAAGTAGGACGAGAGt
    17+[17]+17
human, EXT1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0068 tTCCTCCCAGGGGGATGTcctgcgcctcagggtcCGGTGGTGGCCTGCGGCa
aAGGAGGGTCCCCCTACAggacgcggagtcccagGCCACCACCGGACGCCGt
    17+[16]+17
human, EXT2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0069 tGCTTTTAGAATAATCATgggccagactgggaagAAATCTGAGAAGGGACCa
aCGAAAATCTTATTAGTAcccggtctgacccttcTTTAGACTCTTCCCTGGt
    17+[16]+17
human, EZH2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0070 tAGGCGCCAAGGCCATGTccgactcgtgggtcccGAACTCCGCCTCGGGCCa
aTCCGCGGTTCCGGTACAggctgagcacccagggCTTGAGGCGGAGCCCGGt
    17+[16]+17
human, FANCA, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0071 tGAAGGGACATCACCTTTtcgctttttccaagatgGCTCAAGATTCAGTAGa
aCTTCCCTGTAGTGGAAAagcgaaaaaggttctacCGAGTTCTAAGTCATCt
    17+[17]+16
human, FANCC, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0072 tGCCCCGGCATGGCGACAccggacgcggggctcccTGGGGCTGAGGGCGTGGa
aCGGGGCCGTACCGCTGTggcctgcgccccgagggACCCCGACTCCCGCACCt
    17+[17]+17
human, FANCE, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0073 tTCGCGCACCTCATGGaatcccttctgcagcaCCTGGATCGCTTTTCCGa
aAGCGCGTGGAGTACCttagggaagacgtcgtGGACCTAGCGAAAAGGCt
    15+[16]+17
human, FANCF, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0074 tCGGCCACCATGTCCCgccagaccacctctgtGGGCTCCAGCTGCCTGGa
aGCCGGTGGTACAGGGcggtctggtggagacaCCCGAGGTCGACGGACCt
    15+[16]+17
human, FANCG, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0075 tCCCCAGAACAGCACTATgggcttctcttccgagctGTGCAGCCCCCAGGGCCa
aGGGGTCTTGTCGTGATAcccgaagagaaggctcgaCACGTCGGGGGTCCCGGt
    17+[18]+17
human, FES, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0076 tCTGCTCCCCACCGAGGAcctctgcatgcaggcaTGAATCCCAGGAGCCTa
aGACGAGGGGTGGCTCCTggagacgtacgtccgtACTTAGGGTCCTCGGAt
    17+[16]+16
human, FGFR1 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0077 tGTACCGAGCACTTCGGCtcctcgcgcgctcgcgtCCCCTCGTGCGGGCTCCa
aCATGGCTCGTGAAGCCGaggagcgcgcgagcgcaGGGGAGCACGCCCGAGGt
    17+[17]+17
human, FH, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0078 tCTCCAAGGCACCATGAAtgccatcgtggctctctgCCACTTCTGCGAGCTCCa
aGAGGTTCCGTGGTACTTacggtagcaccgagagacGGTGAAGACGCTCGAGGt
    17+[18]+17
human, FLCN, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0079 tCCGGAGGCCATGCCGGCg...aGCTGCCGCTGCTCGGTa
aGGCCTCCGGTACGGCCGc...tCGACGGCGACGAGCCAt
    17+[21]+16
human, FLT3, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0080 tGCAGCGGGGCGCCGCGCtgtgcctgcgactgtggctCTGCCTGGGACTCCTGGa
aCGTCGCCCCGCGGCGCGacacggacgctgacaccgaGACGGACCCTGAGGACCt
    17+[19]+17
human, FLT4, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0081 tCACCATGGCCGAGGCGcctcaggtggtggagaTCGACCCGGACTTCGa
aGTGGTACCGGCTCCGCggagtccaccacctctAGCTGGGCCTGAAGCt
    16+[16]+15
human, FOXO1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0082 tCTCCGCTCGAAGTGGAGctggacccggagttcgagCCCCAGAGCCGTCCGCGa
aGAGGCGAGCTTCACCTCgacctgggcctcaagctcGGGGTCTCGGCAGGCGCt
    17+[18]+17
human, FOXO3 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0083 tCCTCTGAGACGCCATGTtcaactcgatgaccccACCACCAATCAGTAGCTa
aGGAGACTCTGCGGTACAagttgagctactggggTGGTGGTTAGTCATCGAt
    17+[16]+17
human, GLI1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0084 tGGCGCAGACGCAGGGCacccggaggaaagtctgTTACTACTACGACGGTGa
aCCGCGTCTGCGTCCCGtgggcctcctttcagacAATGATGATGCTGCCACt
    16+[17]+17
human, HDAC1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0085 tGCGCTCACCTCCCTGCGgcctcctgaggtggtttgGTGGCCCCCTCCTCGCGa
aCGCGAGTGGAGGGACGCcggaggactccaccaaacCACCGGGGGAGGAGCGCt
    17+[18]+17
human, HDAC2 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0086 tCCTCAACTATGACCTCAaccggccaggattccaCCACAACCAGGCAGCGAa
aGGAGTTGATACTGGAGTtggccggtcctaaggtGGTGTTGGTCCGTCGCTt
    17+[16]+17
human, HDAC6, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0087 tGAGCGCACGCGGTGAGGgcgcggggcagccgtcCACTTCAGCCCAGGGACa
aCTCGCGTGCGCCACTCCcgcgccccgtcggcagGTGAAGTCGGGTCCCTGt
    17+[16]+17
human, HMGA2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0088 tCCGTGCTCCTCCACCCCcgctggatcgagcccacCGTCATGTTTCTCTACGa
aGGCACGAGGAGGTGGGGgcgacctagctcgggtgGCAGTACAAAGAGATGCt
    17+[17]+17
human, HOXA13 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0089 tGGGCACGGTGATGGCcaccactggggccctgGGCAACTACTACGTGGa
aCCCGTGCCACTACCGgtggtgaccccgggacCCGTTGATGATGCACCt
    15+[16]+16
human, HOXA9, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0090 tCCAGCAGATCATGTCATgacgacttcgctgctcctGCATCCACGCTGGCCGGa
aGGTCGTCTAGTACAGTActgctgaagcgacgaggaCGTAGGTGCGACCGGCCt
    17+[18]+17
human, HOXC13, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0091 tTGACGAGTGCGGCCAGagcgcagccagcatgtaCCTGCCGGGCTGCGCCTa
aACTGCTCACGCCGGTCtcgcgtcggtcgtacatGGACGGCCCGACGCGGAt
    16+[17]+17
human, HOXD11 G C (std.) +63 WT FLASH 22484455 R No
TN0092 tGCGGGCAGACGGCGGGGgcgccggtggcgccccgGCCTCTTCCTCCTCCTCa
aCGCCCGTCTGCCGCCCCcgcggccaccgcggggcCGGAGAAGGAGGAGGAGt
    17+[17]+17
human, HOXD13 G C (std.) +63 WT FLASH 22484455 R No
TN0093 tCTGAAAAAGACTCTGCAtgggaatggcctgcctTACGATGACAGAAATGGa
aGACTTTTTCTGAGACGTacccttaccggacggaATGCTACTGTCTTTACCt
    17+[16]+17
human, JAK2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0094 tACCGCGATGAGAGGCGCtcgcggcgcctgggattttCTCTGCGTTCTGCTCCTa
aTGGCGCTACTCTCCGCGagcgccgcggaccctaaaaGAGACGCAAGACGAGGAt
    17+[19]+17
human, KIT, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0095 tGAAAATGACTGAATATAaacttgtggtagttggaGCTGGTGGCGTAGGCAa
aCTTTTACTGACTTATATttgaacaccatcaacctCGACCACCGCATCCGTt
    17+[17]+16
human, KRAS, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0096 tAGGGTCCCCGGCGCCAGgccacccggccgtcagCAGCATGCAGGGTAAGGa
aTCCCAGGGGCCGCGGTCcggtgggccggcagtcGTCGTACGTCCCATTCCt
    17+[16]+17
human, MAP2K4 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0097 tCCAAGCGCGAAAACCCCggatggtgaggagcaggTACTGGCCCGGCAGCGa
aGGTTCGCGCTTTTGGGGcctaccactcctcgtccATGACCGGGCCGTCGCt
    17+[17]+16
human, MDM2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0098 tTATTATTACATGGCTTTgccttactgaggcttcATCTTGTCCTCTGGTCCa
aATAATAATGTACCGAAAcggaatgactccgaagTAGAACAGGAGACCAGGt
    17+[16]+17
human, MET G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0099 tCTGGCGCCAAAATGTCGttcgtggcaggggttaTTCGGCGGCTGGACGAGa
aGACCGCGGTTTTACAGCaagcaccgtccccaatAAGCCGCCGACCTGCTCt
    17+[16]+17
human, MLH1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0100 tGAGGAGGTTTCGACATGgcggtgcagccgaaggAGACGCTGCAGTTGGAGa
aCTCCTCCAAAGCTGTACcgccacgtcggcttccTCTGCGACGTCAACCTCt
    17+[16]+17
human, MSH2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0101 tCACTGTCGGCGGCCATGacaccgctcgtctcccgcCTGAGTCGTCTGTGGGTa
aGTGACAGCCGCCGGTACtgtggcgagcagagggcgGACTCAGCAGACACCCAt
    17+[18]+17
human, MUTYH, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0102 tGCTTAGACGCTGGATTTttttcgggtagtggaaAACCAGGTAAGCACCGAa
aCGAATCTGCGACCTAAAaaaagcccatcaccttTTGGTCCATTCGTGGCTt
    17+[16]+17
human, MYC G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0103 tCCCGCAGGGAGCGGACAtggactacgactcgtaCCAGCACTATTTCTACGa
aGGGCGTCCCTCGCCTGTacctgatgctgagcatGGTCGTGATAAAGATGCt
    17+[16]+17
human, MYCL1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0104 tGCCGAGCTGCTCCACgtccaccatgccgggcATGATCTGCAAGAACCCa
aCGGCTCGACGAGGTGcaggtggtacggcccgTACTAGACGTTCTTGGGt
    15+[16]+17
human, MYCN, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0105 tGAGGAGCCGGACCGAtgtggaaactgctgccCGCCGCGGGCCCGGCa
aCTCCTCGGCCTGGCTacacctttgacgacggGCGGCGCCCGGGCCGt
    15+[16]+15
human, NBN, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0106 tCTTTACTGATAATGTCAagttcaggttatcctcCCAACCAAGGAGCATTCa
aGAAATGACTATTACAGTtcaagtccaataggagGGTTGGTTCCTCGTAAGt
    17+[16]+17
human, NCOR1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0107 tGGAGGGCCACTGAGCcccgctacccgccccaCAGCCTTTCCTACCCa
aCCTCCCGGTGACTCGgggcgatgggcggggtGTCGGAAAGGATGGGt
    15+[16]+15
human, NCOR2 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0108 tCGGCGCATGAAGGAGGTactcctcattttcgttCTCTCTCTCTGTGCCCCa
aGCCGCGTACTTCCTCCAtgaggagtaaaagcaaGAGAGAGAGACACGGGGt
    17+[16]+17
human, NTRK1, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0109 tTGCGCTCGGGGCGGCCAtgtcggccggcgaggtCGAGCGCCTAGTGTCGGa
aACGCGAGCCCCGCCGGTacagccggccgctccaGCTCGCGGATCACAGCCt
    17+[16]+17
human, PDGFRA, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0110 tCTGCAGGACACCATGCGgcttccgggtgcgatgCCAGCTCTGGCCCTCAAa
aGACGTCCTGTGGTACGCcgaaggcccacgctacGGTCGAGACCGGGAGTTt
    17+[16]+17
human, PDGFRB, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0111 tGAGTACTCCGCCTCTACcccggctgaagcccgcCCCCGCCGCCACCTATTa
aCTCATGAGGCGGAGATGgggccgacttcgggcgGGGGCGGCGGTGGATAAt
    17+[16]+17
human, PHF8 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0112 tCGGGTGTTGCATCCATGgagcgagctgagagctcgAGGTGAGCGGGGCTCGCa
aGCCCACAACGTAGGTACctcgctcgactctcgagcTCCACTCGCCCCGAGCGt
    17+[18]+17
human, PMS2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0113 tGGAACTGCTTAATAGaaacaggcttgtaattGTGAGTCCGCGCTGCa
aCCTTGACGAATTATCtttgtccgaacattaaCACTCAGGCGCGACGt
    15+[16]+15
human, PTCH1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0114 tCCCAGACATGACAGCCatcatcaaagagatcgTTAGCAGAAACAAAAGGa
aGGGTCTGTACTGTCGGtagtagtttctctagcAATCGTCTTTGTTTTCCt
    16+[16]+17
human, PTEN, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0115 tGGCATGGCCAGCAACAGcagctcctgcccgacacCTGGGGGCGGGCACCTCa
aCCGTACCGGTCGTTGTCgtcgaggacgggctgtgGACCCCCGCCCGTGGAGt
    17+[17]+17
human, RARA, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0116 tGCTGGGTGAGAAGGGCtgtggctgcgttttagaGAAGCGTTGGGTACTGGa
aCGACCCACTCTTCCCGacaccgacgcaaaatctCTTCGCAACCCATGACCt
    16+[17]+17
human, RBBP5 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0117 tGCGGGACGTGCGGGAGCggctgcaggcgtgggaGCGCGCGTTCCGACGGCa
aCGCCCTGCACGCCCTCGccgacgtccgcaccctCGCGCGCAAGGCTGCCGt
    17+[16]+17
human, RECQL4 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0118 tCAGAATACAGTTATGGCcacccaggtaatggggCAGTCTTCTGGAGGAGGa
aGTCTTATGTCAATACCGgtgggtccattaccccGTCAGAAGACCTCCTCCt
    17+[16]+17
human, REST, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0119 tGAGTTCTGCCGGCCGCCggctcccgcaggggccaGGGCGAAGTTGGCGCCGa
aCTCAAGACGGCCGGCGGccgagggcgtccccggtCCCGCTTCAACCGCGGCt
    17+[17]+17
human, RET G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0120 tTCTTTATTTCCAGCAATgtctcaggctgtgcagACAAACGGAACTCAACCa
aAGAAATAAAGGTCGTTAcagagtccgacacgtcTGTTTGCCTTGAGTTGGt
    17+[16]+17
human, RNF2, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0121 tTCAGGAGGAAGCGATGGcttcagacagcatattTGAGTCATTTCCTTCGTa
aAGTCCTCCTTCGCTACCgaagtctgtcgtataaACTCAGTAAAGGAAGCAt
    17+[16]+17
human, RUNX1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0122 tCTCCTTGAGGCGCCGGTtgccggccacaaccctTGGCGGAGCCTGCCTGCa
aGAGGAACTCCGCGGCCAacggccggtgttgggaACCGCCTCGGACGGACGt
    17+[16]+17
human, SDHB G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0123 tGTTGCTGAGGTGACTTCagtgggactgggagttggtGCCTGCGGCCCTCCGGa
aCAACGACTCCACTGAAGtcaccctgaccctcaaccaCGGACGCCGGGAGGCCt
    17+[19]+16
human, SDHC G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0124 tCAGGAACGAGATGGCGGttctctggaggctgagtGCCGTTTGCGGTGCCCTa
aGTCCTTGCTCTACCGCCaagagacctccgactcaCGGCAAACGCCACGGGAt
    17+[17]+17
human, SDHD, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0125 tGCAGAGGACAAAAGCATgtcttcccttcctgggTGCATTGGTTTGGATGCa
aCGTCTCCTGTTTTCGTAcagaagggaaggacccACGTAACCAAACCTACGt
    17+[16]+17
human, SETDB1, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0126 tTACGCGGCGGGGCTGTCgccgtacgcggacaagggCAAGTGCGGCCTCCCGGa
aATGCGCCGCCCCGACAGcggcatgcgcctgttcccGTTCACGCCGGAGGGCCt
    17+[18]+17
human, SIRT6 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0127 tTTGGTAAGAACATGTCGtccatcttgccattcacGCCGCCAGTTGTGAAGa
aAACCATTCTTGTACAGCaggtagaacggtaagtgCGGCGGTCAACACTTCt
    17+[17]+16
human, SMAD2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0128 tGGTGACGGCGGCAACATgtctgtggctttcgcggCCCCGAGGCAGCGAGGCa
aCCACTGCCGCCGTTGTAcagacaccgaaagcgccGGGGCTCCGTCGCTCCGt
    17+[17]+17
human, SS18, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0129 tGGCGCCTCAGAAGCAcggcggtgggggagggGGCGGCTCGGGGCCCa
aCCGCGGAGTCTTCGTgccgccaccccctcccCCGCCGAGCCCCGGGt
    15+[16]+15
human, SUZ12, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0130 tCATGTCTCATGCGGCCGaaccagctcgggatggCGTAGAGGCCAGCGCGGa
aGTACAGAGTACGCCGGCttggtcgagccctaccGCATCTCCGGTCGCGCCt
    17+[16]+17
human, TFE3, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0131 tCGGGGGCTGCTCAGGGGcctgtggccgctgcacaTCGTCCTGTGGACGCGTa
aGCCCCCGACGAGTCCCCggacaccggcgacgtgtAGCAGGACACCTGCGCAt
    17+[17]+17
human, TGFBR2 G C (std.) +63 WT FLASH 22484455 R No
TN0132 tTCCGCCCGCCCAGGATGgaggcgcccgccagcgcGCAGACCCCGCACCCGCa
aAGGCGGGCGGGTCCTACctccgcgggcggtcgcgCGTCTGGGGCGTGGGCGt
    17+[17]+17
human, TLX3, ATG G C (std.) +63 WT FLASH 22484455 R No
TN0133 tTGCCGTCCCAAGCAATGgatgatttgatgctgtcCCCGGACGATATTGAACa
aACGGCAGGGTTCGTTACctactaaactacgacagGGGCCTGCTATAACTTGt
    17+[17]+17
human, TP53 G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0134 tCCTGGTCCACCATGGCcaaaccaacaagcaaagATTCAGGCTTGAAGGAGa
aGGACCAGGTGGTACCGgtttggttgttcgtttcTAAGTCCGAACTTCCTCt
    16+[17]+17
human, TSC2, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0135 tCTGGATCGCGGAGGGAAtgccccggagggcggaGAACTGGGACGAGGCCGa
aGACCTAGCGCCTCCCTTacggggcctcccgcctCTTGACCCTGCTCCGGCt
    17+[16]+17
human, VHL, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0136 tGGGCCAGAGATGGCGGCggccgacggggctttgCCGGAGGCGGCGGCTTTa
aCCCGGTCTCTACCGCCGccggctgccccgaaacGGCCTCCGCCGCCGAAAt
    17+[16]+17
human, XPA, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0137 tGCCCAGACAAGCAACATggctcggaaacgcgcggccGGCGGGGAGCCGCGGGGa
aCGGGTCTGTTCGTTGTAccgagcctttgcgcgccggCCGCCCCTCGGCGCCCCt
    17+[19]+17
human, XPC, ATG G C (std.) +63 WT FLASH NHEJ 22484455 R
TN0146 tGCTCAAACCCGACGAGCatcaccacatcgaggacgAGCACGTGAGGGCGCCCa
aCGAGTTTGGGCTGCTCGtagtggtgtagctcctgcTCGTGCACTCCCGCGGGt
    17+[18]+17
zebrafish, myod1 G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0147 tATTTCTGCGCAGAAGTGgccccaccaagcctgctGGAGGACATACAGAATTa
aTAAAGACGCGTCTTCACcggggtggttcggacgaCCTCCTGTATGTCTTAAt
    17+[17]+17
zebrafish, jak3 G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0145 tCAGCCTTGGTCACATCTcattcagacagtgaaaACATCGGAGGCTTTTCTa
aGTCGGAACCAGTGTAGAgtaagtctgtcactttTGTAGCCTCCGAAAAGAt
    17+[16]+17
zebrafish, phf6 G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0144 tGGAGTGTATGAAAACTGgtcatgtcaggacagaGAAAAGGAGCTTCAGCa
aCCTCACATACTTTTGACcagtacagtcctgtctCTTTTCCTCGAAGTCGt
    17+[16]+16
zebrafish, phf6, ATG G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0142 tTCTTTTCTATCCCATGAcgatgcatcgcacaacAAGGATCAAGATTACAGa
aAGAAAAGATAGGGTACTgctacgtagcgtgttgTTCCTAGTTCTAATGTCt
    17+[16]+17
zebrafish, bmi1a, ATG G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0143 tACCTGCACAGGATGCAgactgaggaggcacagGAAATGTCCCAGATAACa
aTGGACGTGTCCTACGTctgactcctccgtgtcCTTTACAGGGTCTATTGt
    16+[16]+17
zebrafish, ikzf1, ATG G C (std.) +63 WT FLASH NHEJ 22655075 R
TN0150 tACAATACTCCCACTGAAatgacagatgcagacagACTCATGGACAGTTGGTa
aTGTTATGAGGGTGACTTtactgtctacgtctgtcTGAGTACCTGTCAACCAt
    17+[17]+17
zebrafish, epas1b G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0149 tCAGAGCGGCGTAAGGAGaaatctcgtgatgccgCGCGCTGCAGACGGAGTa
aGTCTCGCCGCATTCCTCtttagagcactacggcGCGCGACGTCTGCCTCAt
    17+[16]+17
zebrafish, epas1b G C (std.) +63 WT; ELD+KKR FLASH (Inactive) 22684503 R No
TN0148 tCCGGCAGACATCGTGAAggtggccatcgagtggCCTGGGGCCTTCCCCAAa
aGGCCGTCTGTAGCACTTccaccggtagctcaccGGACCCCGGAAGGGGTTt
    17+[16]+17
zebrafish, elmo1 G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0150-T002 tACGCTACTCCCACTGAAatgacagatgcagacagACTCATGGACAGTTGGTa
aTGCGATGAGGGTGACTTtactgtctacgtctgtcTGAGTACCTGTCAACCAt
    17+[17]+17
zebrafish, epas1b [mutated] G C +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0151 tTTGATGGATCTCTGAGCggcccgcagatctgacAAACTCGCGCTGAAGCGa
aAACTACCTAGAGACTCGccgggcgtctagactgTTTGAGCGCGACTTCGCt
    17+[16]+17
zebrafish, fh G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0152 tACTGGAGTTGTCACTGAaaagaaaaggtagggaTTCCCGTTTTTGCTTGGa
aTGACCTCAACAGTGACTtttcttttccatccctAAGGGCAAAAACGAACCt
    17+[16]+17
zebrafish, hif1ab, exon-intron G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0153 tCGGAGCGCAGGAAGGAGaagtccagggatgcagCGCGATCTCGCAGGGGAa
aGCCTCGCGTCCTTCCTCttcaggtccctacgtcGCGCTAGAGCGTCCCCTt
    17+[16]+17
zebrafish, hif1ab G C (std.) +63 WT; ELD+KKR FLASH (Inactive) 22684503 R No
TN0154 TTCGTTTATACGAGGAACcatgtcgagtgttttAGCGAGAATTTTATTCTA
AAGCAAATATGCTCCTTGgtacagctcacaaaaTCGCTCTTAAAATAAGAT
    18+[15]+18
zebrafish, ptpmt1 G C (std.) +63 WT FLASH (Inactive) 22684503 R No
TN0155 tGGTCCAAAATGAAAAAGtaagaggagtaattacCATGAATGAAGAATATGa
aCCAGGTTTTACTTTTTCattctcctcattaatgGTACTTACTTCTTATACt
    17+[16]+17
zebrafish, ptpmt1 G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R
TN0156 TTGAGCAGATCAGATTGGacactgtggacctgacAGGTGTTCCCAGTCTTGA
AACTCGTCTAGTCTAACCtgtgacacctggactgTCCACAAGGGTCAGAACT
    18+[16]+18
zebrafish, ptpmt1 G C (std.) +63 WT FLASH NHEJ 22684503 R
TN0157 tAGAAGAGCGGCATCCCGgcgatcaccatgaagaAGAGATACGGCACCAGGa
aTCTTCTCGCCGTAGGGCcgctagtggtacttctTCTCTATGCCGTGGTCCt
    17+[16]+17
zebrafish, slc6a3 G C (std.) +63 WT; ELD+KKR FLASH NHEJ 22684503 R




Legends
Off-target or secondary target
EEN targeted same or overlapped site with another reported EEN
EEN reported with low or no activity
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