EENdb - TALEN/ZFN  >  ZFN detail
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ZN0143
IDZN0143
Type / formZFN, dimer. (Z-D)
More about ZFN forms
Target Full siteaTACGCCtCGCCCGAGCtgtgcgGTAGGCATTGAGGCAGCCa
tATGCGGaGCGGGCTCGacacgcCATCCGTAACTCCGTCGGt
LengthLeft+[Spacer]+Right: (3F+1+2F)+[6]+6F
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusMALAT1 G
Location to genepromoter
ZFNs Left ZFP(s)ZP10764 (F1->Fn): SSGGLNR DTSHLSR RSNDLTR RSDNLSE TSGSLTR
Target (5'->3'): GCTCGGGCGaGGCGTA
Right ZFP(s)ZP10765 (F1->Fn): ERGTLAR QSGDLTR RSDTLSV HSNARKT DRSHLTR QSGSLTR
Target (5'->3'): GTAGGCATTGAGGCAGCC
ZFN framework &
ZFP-FokI linker peptide
Framework: (N/A)
Linker peptide: LRGS

More about ZFN frameworks & linker peptides
FokI variantEL+KK
List EENs with FokI variants EL+KK   More about FokI variants
Construction strategySangamo
List EENs constructed by Sangamo   More about ZFP construction strategies
Effectiveness
(first reported)
Genome modification
method
NHEJ; HR
More about modification methods
EfficiencyNHEJ: 9.1-18.5%, mismatch-detection nuclease assay, in K562 cells. HR: +, direct PCR, in A549, K562 cells.
More about efficiency detection methods
Heritability
Specificity
CommentsFull sequence provided.
Reference (first reported)Gutschner et al., Genome Res., 2011 (21844124 R)




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