EENdb - TALEN/ZFN  >  ZFN detail
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ZN0137
IDZN0137
Type / formZFN, dimer. (Z-D)
More about ZFN forms
Target Full sitetCGGTGGCTCAGCTACaggcctGGTGGTACAAGGCACa
aGCCACCGAGTCGATGtccggaCCACCATGTTCCGTGt
LengthLeft+[Spacer]+Right: 5F+[6]+5F
Speicespig (Sus scrofa)
List EENs targeting genome of pig
Gene / LocusGGTA1 G
Location to geneexon
ZFNs Left ZFP(s)ZP10753 (F1->Fn): RSDTLSE QKATRIT RSHNLAR QSSDLSR QSGSLTR
Target (5'->3'): GTAGCTGAGCCACCG
Right ZFP(s)ZP10754 (F1->Fn): DQSNLRA RSDHLSA QSATRTN TSGHLSR TSGHLSR
Target (5'->3'): GGTGGTACAAGGCAC
ZFN framework &
ZFP-FokI linker peptide
Framework: (N/A)
Linker peptide: LRGS

More about ZFN frameworks & linker peptides
FokI variantEL+KK
List EENs with FokI variants EL+KK   More about FokI variants
Construction strategySangamo
List EENs constructed by Sangamo   More about ZFP construction strategies
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency8%/34% (37C/30C), mismatch-detection nuclease assay, in PK(15), fibroblasts cells.
More about efficiency detection methods
Heritability
Specificity39 portential off-targets tested and none showed cleavage.
Comments
Reference (first reported)Hauschild et al., Proc Natl Acad Sci U S A., 2011 (21730124 R)




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