EENdb - TALEN/ZFN  >  ZFN detail
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ZN0128
IDZN0128
Type / formZFN, dimer. (Z-D)
More about ZFN forms
Target Full sitecAGCCGGACCgtCAGGACcttcccGGTGGTGAAGAGAGGGGAc
gTCGGCCTGGcaGTCCTGgaagggCCACCACTTCTCTCCCCTg
LengthLeft+[Spacer]+Right: (2F+2+3F)+[6]+6F
Speicesrabbit (Oryctolagus cuniculus)
List EENs targeting genome of rabbit
Gene / LocusIGHM (IgM) G
Location to geneexon
ZFNs Left ZFP(s)ZP10730 (F1->Fn): QSSDLSR RSDDRKT TSGHLSR RSDTLSQ DRSARTR
Target (5'->3'): GTCCTGacGGTCCGGCT
Right ZFP(s)ZP10731 (F1->Fn): QSGHLAR RSDHLTQ RSANLAR QSGNLAR TSGHLSR TSGHLSR
Target (5'->3'): GGTGGTGAAGAGAGGGGA
ZFN framework &
ZFP-FokI linker peptide
Framework: (N/A)
Linker peptide: (N/A)

More about ZFN frameworks & linker peptides
FokI variant(N/A)
More about FokI variants
Construction strategySangamo
List EENs constructed by Sangamo   More about ZFP construction strategies
Effectiveness
(first reported)
Genome modification
method
NHEJ; HR
More about modification methods
EfficiencyNHEJ: +, direct sequencing; HR: +, reporter gene addition assay & direct PCR & Southern blotting.
More about efficiency detection methods
Heritability
Specificity
Comments
Reference (first reported)Flisikowska et al., PLoS One., 2011 (21695153 R)




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