EENdb - TALEN/ZFN  >  ZFN detail
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ZN0092
IDZN0092
Type / formZFN, dimer. (Z-D)
More about ZFN forms
Target Full sitetCAAATCTGCCGTGTAtgtgggGACAAGGCCaATGGCTa
aGTTTAGACGGCACATacacccCTGTTCCGGtTACCGAt
LengthLeft+[Spacer]+Right: 5F+[6]+(3F+1+2F)
Speicesrat (Rattus norvegicus)
List EENs targeting genome of rat
Gene / LocusNr1i2 (PXR) G
Location to geneexon
ZFNs Left ZFP(s)ZP10555 (F1->Fn): DNAALTE TSSNLSR QSGDLTR RSDTLSQ DNANRTK
Target (5'->3'): TACACGGCAGATTTG
Right ZFP(s)ZP10556 (F1->Fn): QSSDLSR RSDALTQ DRSDLSR RSDNLSV DRSNLTR
Target (5'->3'): GACAAGGCCaATGGCT
ZFN framework &
ZFP-FokI linker peptide
Framework: (N/A)
Linker peptide: LRGS

More about ZFN frameworks & linker peptides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction strategySangamo
List EENs constructed by Sangamo   More about ZFP construction strategies
Effectiveness
(first reported)
Genome modification
method
NHEJ; HR
More about modification methods
Efficiency+ (see ref.), PCR-RFLP & Southern blot.
More about efficiency detection methods
Heritability
Specificity
CommentsFull sequence provided.
Reference (first reported)Cui et al., Nat Biotechnol., 2011 (21151125 R)




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