EENdb - TALEN/ZFN  >  ZFN detail
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ZN0088
IDZN0088
Type / formZFN, dimer. (Z-D)
More about ZFN forms
Target Full sitegAGTCTCAGCacagaaGACATAGAAa
cTCAGAGTCGtgtcttCTGTATCTTt
LengthLeft+[Spacer]+Right: 3F+[6]+3F
Speicessilk worm (Bombyx mori)
List EENs targeting genome of silk worm
Gene / LocusBmBLOS2 G
Location to geneexon
ZFNs Left ZFP(s)ZP10549 (F1->Fn): THLDLIR RSDNLAR QSSDLTR
Target (5'->3'): GCTGAGACT
Right ZFP(s)ZP10548 (F1->Fn): QSGNLAR QKSSLTA DRSNLTR
Target (5'->3'): GACATAGAA
ZFN framework &
ZFP-FokI linker peptide
Framework: (N/A)
Linker peptide: TGQK

More about ZFN frameworks & linker peptides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction strategyMA
List EENs constructed by MA   More about ZFP construction strategies
Effectiveness
(first reported)
Genome modification
method
NHEJ
More about modification methods
Efficiency+, reporter gene disruption assay & direct sequencing.
More about efficiency detection methods
Heritability
Specificity
CommentsFull sequence provided.
Reference (first reported)Takasu et al., Insect Biochem Mol Biol., 2010 (20692340 R)




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